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Protein

Glycylpeptide N-tetradecanoyltransferase 1

Gene

Nmt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins. Required for normal embryogenesis.1 Publication

Catalytic activityi

Tetradecanoyl-CoA + glycylpeptide = CoA + N-tetradecanoylglycylpeptide.1 Publication

GO - Molecular functioni

GO - Biological processi

  • in utero embryonic development Source: MGI
  • N-terminal peptidyl-glycine N-myristoylation Source: UniProtKB
  • N-terminal protein myristoylation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.3.1.97. 3474.
ReactomeiR-MMU-2514859. Inactivation, recovery and regulation of the phototransduction cascade.
R-MMU-75108. Activation, myristolyation of BID and translocation to mitochondria.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycylpeptide N-tetradecanoyltransferase 1 (EC:2.3.1.971 Publication)
Alternative name(s):
Myristoyl-CoA:protein N-myristoyltransferase 1
Short name:
NMT 1
Short name:
Type I N-myristoyltransferase
Peptide N-myristoyltransferase 1
Gene namesi
Name:Nmt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:102579. Nmt1.

Subcellular locationi

  • Cytoplasm By similarity
  • Cytoplasmcytosol By similarity
  • Membrane By similarity; Peripheral membrane protein By similarity

  • Note: Copurifies with ribosomes.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Complete embryonic lethality. Embryos die between 3.5 and 7.5 dpc.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 496496Glycylpeptide N-tetradecanoyltransferase 1PRO_0000064222Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei31 – 311PhosphoserineCombined sources
Modified residuei47 – 471PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO70310.
PaxDbiO70310.
PRIDEiO70310.

PTM databases

iPTMnetiO70310.
PhosphoSiteiO70310.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiO70310.
CleanExiMM_NMT1.
ExpressionAtlasiO70310. baseline and differential.
GenevisibleiO70310. MM.

Interactioni

Protein-protein interaction databases

BioGridi201793. 1 interaction.
IntActiO70310. 2 interactions.
MINTiMINT-1847729.
STRINGi10090.ENSMUSP00000021314.

Structurei

3D structure databases

ProteinModelPortaliO70310.
SMRiO70310. Positions 115-496.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni118 – 1203Myristoyl-CoA bindingBy similarity
Regioni248 – 2503Myristoyl-CoA bindingBy similarity
Regioni256 – 2605Myristoyl-CoA bindingBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi55 – 6713Poly-LysAdd
BLAST

Sequence similaritiesi

Belongs to the NMT family.Curated

Phylogenomic databases

eggNOGiKOG2779. Eukaryota.
COG5092. LUCA.
HOGENOMiHOG000189123.
HOVERGENiHBG003404.
InParanoidiO70310.
KOiK00671.
OMAiTIMNHPV.
OrthoDBiEOG76DTS3.
PhylomeDBiO70310.
TreeFamiTF300701.

Family and domain databases

Gene3Di3.40.630.30. 2 hits.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000903. MyristoylCoA_TrFase.
IPR022677. MyristoylCoA_TrFase_C.
IPR022678. MyristoylCoA_TrFase_CS.
IPR022676. MyristoylCoA_TrFase_N.
[Graphical view]
PANTHERiPTHR11377. PTHR11377. 1 hit.
PfamiPF01233. NMT. 1 hit.
PF02799. NMT_C. 1 hit.
[Graphical view]
PIRSFiPIRSF015892. N-myristl_transf. 1 hit.
SUPFAMiSSF55729. SSF55729. 2 hits.
PROSITEiPS00975. NMT_1. 1 hit.
PS00976. NMT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O70310-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADESETAVK LPAPSLPLMM EGNGNGHEHC SDCENEEDNS HNRSGLSPAN
60 70 80 90 100
DTGAKKKKKK QKKKKEKGSD MESTQDQPVK MTSLPAERIQ EIQKAIELFS
110 120 130 140 150
VGQGPAKTME EASKRSYQFW DTQPVPKLGE VVNTHGPVEP DKDNIRQEPY
160 170 180 190 200
TLPQGFTWDA LDLGDRGVLK ELYTLLNENY VEDDDNMFRF DYSPEFLLWA
210 220 230 240 250
LRPPGWLPQW HCGVRVVSSR KLVGFISAIP ANIHIYDTEK KMVEINFLCV
260 270 280 290 300
HKKLRSKRVA PVLIREITRR VHLEGIFQAV YTAGVVLPKP VGTCRYWHRS
310 320 330 340 350
LNPRKLIEVK FSHLSRNMTM QRTMKLYRLP ETPKTAGLRP MEKKDIPVVH
360 370 380 390 400
QLLSRYLKQF HLTPVMNQEE VEHWFYPQEN IIDTFVVENA NGEVTDFLSF
410 420 430 440 450
YTLPSTIMNH PTHKSLKAAY SFYNVHTQTP LLDLMSDALV LAKMKGFDVF
460 470 480 490
NALDLMENKT FLEKLKFGIG DGNLQYYLYN WKCPSMGAEK VGLVLQ
Length:496
Mass (Da):56,888
Last modified:August 1, 1998 - v1
Checksum:i61FA7B854A5CF3BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043326 mRNA. Translation: AAC09296.1.
BC016526 mRNA. Translation: AAH16526.1.
BC021635 mRNA. Translation: AAH21635.1.
CCDSiCCDS25511.1.
RefSeqiNP_032733.1. NM_008707.3.
XP_006532508.1. XM_006532445.2.
UniGeneiMm.10265.

Genome annotation databases

EnsembliENSMUST00000021314; ENSMUSP00000021314; ENSMUSG00000020936.
GeneIDi18107.
KEGGimmu:18107.
UCSCiuc007ltd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043326 mRNA. Translation: AAC09296.1.
BC016526 mRNA. Translation: AAH16526.1.
BC021635 mRNA. Translation: AAH21635.1.
CCDSiCCDS25511.1.
RefSeqiNP_032733.1. NM_008707.3.
XP_006532508.1. XM_006532445.2.
UniGeneiMm.10265.

3D structure databases

ProteinModelPortaliO70310.
SMRiO70310. Positions 115-496.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201793. 1 interaction.
IntActiO70310. 2 interactions.
MINTiMINT-1847729.
STRINGi10090.ENSMUSP00000021314.

PTM databases

iPTMnetiO70310.
PhosphoSiteiO70310.

Proteomic databases

EPDiO70310.
PaxDbiO70310.
PRIDEiO70310.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021314; ENSMUSP00000021314; ENSMUSG00000020936.
GeneIDi18107.
KEGGimmu:18107.
UCSCiuc007ltd.1. mouse.

Organism-specific databases

CTDi4836.
MGIiMGI:102579. Nmt1.

Phylogenomic databases

eggNOGiKOG2779. Eukaryota.
COG5092. LUCA.
HOGENOMiHOG000189123.
HOVERGENiHBG003404.
InParanoidiO70310.
KOiK00671.
OMAiTIMNHPV.
OrthoDBiEOG76DTS3.
PhylomeDBiO70310.
TreeFamiTF300701.

Enzyme and pathway databases

BRENDAi2.3.1.97. 3474.
ReactomeiR-MMU-2514859. Inactivation, recovery and regulation of the phototransduction cascade.
R-MMU-75108. Activation, myristolyation of BID and translocation to mitochondria.

Miscellaneous databases

ChiTaRSiNmt1. mouse.
PROiO70310.
SOURCEiSearch...

Gene expression databases

BgeeiO70310.
CleanExiMM_NMT1.
ExpressionAtlasiO70310. baseline and differential.
GenevisibleiO70310. MM.

Family and domain databases

Gene3Di3.40.630.30. 2 hits.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000903. MyristoylCoA_TrFase.
IPR022677. MyristoylCoA_TrFase_C.
IPR022678. MyristoylCoA_TrFase_CS.
IPR022676. MyristoylCoA_TrFase_N.
[Graphical view]
PANTHERiPTHR11377. PTHR11377. 1 hit.
PfamiPF01233. NMT. 1 hit.
PF02799. NMT_C. 1 hit.
[Graphical view]
PIRSFiPIRSF015892. N-myristl_transf. 1 hit.
SUPFAMiSSF55729. SSF55729. 2 hits.
PROSITEiPS00975. NMT_1. 1 hit.
PS00976. NMT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A second mammalian N-myristoyltransferase."
    Giang D.K., Cravatt B.F.
    J. Biol. Chem. 273:6595-6598(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary gland.
  3. Cited for: DISRUPTION PHENOTYPE, FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31 AND SER-47, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiNMT1_MOUSE
AccessioniPrimary (citable) accession number: O70310
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: June 8, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.