Reviewed, UniProtKB/Swiss-Prot O70310 (NMT1_MOUSE)

Names and origin

Protein names

Recommended name:
    Glycylpeptide N-tetradecanoyltransferase 1
    EC=2.3.1.97
Alternative name(s):
    Peptide N-myristoyltransferase 1
    Myristoyl-CoA:protein N-myristoyltransferase 1
      Short name=NMT 1
      Short name=Type I N-myristoyltransferase

Gene names

Name:

Nmt1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins.
Catalytic activity	Tetradecanoyl-CoA + glycylpeptide = CoA + N-tetradecanoylglycylpeptide.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the NMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	N-terminal protein myristoylation Inferred from mutant phenotype. Source: MGI in utero embryonic development Inferred from mutant phenotype. Source: MGI
Cellular component	cytoplasm Ref.1 Traceable author statement. Source: MGI
Molecular function	glycylpeptide N-tetradecanoyltransferase activity Inferred from mutant phenotype. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 496	496	Glycylpeptide N-tetradecanoyltransferase 1		PRO_0000064222
Regions
	Compositional bias	55 – 67	13	Poly-Lys
Amino acid modifications
	Modified residue	31	1	Phosphoserine Ref.3
	Modified residue	47	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O70310-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 61FA7B854A5CF3BC	FASTA	496	56,888
	10 20 30 40 50 60 MADESETAVK LPAPSLPLMM EGNGNGHEHC SDCENEEDNS HNRSGLSPAN DTGAKKKKKK 70 80 90 100 110 120 QKKKKEKGSD MESTQDQPVK MTSLPAERIQ EIQKAIELFS VGQGPAKTME EASKRSYQFW 130 140 150 160 170 180 DTQPVPKLGE VVNTHGPVEP DKDNIRQEPY TLPQGFTWDA LDLGDRGVLK ELYTLLNENY 190 200 210 220 230 240 VEDDDNMFRF DYSPEFLLWA LRPPGWLPQW HCGVRVVSSR KLVGFISAIP ANIHIYDTEK 250 260 270 280 290 300 KMVEINFLCV HKKLRSKRVA PVLIREITRR VHLEGIFQAV YTAGVVLPKP VGTCRYWHRS 310 320 330 340 350 360 LNPRKLIEVK FSHLSRNMTM QRTMKLYRLP ETPKTAGLRP MEKKDIPVVH QLLSRYLKQF 370 380 390 400 410 420 HLTPVMNQEE VEHWFYPQEN IIDTFVVENA NGEVTDFLSF YTLPSTIMNH PTHKSLKAAY 430 440 450 460 470 480 SFYNVHTQTP LLDLMSDALV LAKMKGFDVF NALDLMENKT FLEKLKFGIG DGNLQYYLYN 490 WKCPSMGAEK VGLVLQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A second mammalian N-myristoyltransferase." Giang D.K., Cravatt B.F. J. Biol. Chem. 273:6595-6598(1998) [PubMed: 9506952] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II. Tissue: Mammary gland.
[3]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF043326 mRNA. Translation: AAC09296.1. BC016526 mRNA. Translation: AAH16526.1. BC021635 mRNA. Translation: AAH21635.1.
IPI	IPI00224128.
RefSeq	NP_032733.1.
UniGene	Mm.10265
3D structure databases
HSSP	HSSP built from PDB template 1IYL based on UniProtKB P30418.
ModBase	Search...
Protein-protein interaction databases
STRING	O70310.
PTM databases
PhosphoSite	O70310.
Proteomic databases
PRIDE	O70310.
Genome annotation databases
Ensembl	ENSMUST00000021314; ENSMUSP00000021314; ENSMUSG00000020936; Mus musculus. [Genome view]
GeneID	18107.
KEGG	mmu:18107.
UCSC	uc007ltd.1. mouse.
Organism-specific databases
CTD	18107.
MGI	MGI:102579. Nmt1.
Phylogenomic databases
HOGENOM	O70310.
HOVERGEN	O70310.
OMA	YRLPETP.
Enzyme and pathway databases
BRENDA	2.3.1.97. 244.
Gene expression databases
ArrayExpress	O70310.
Bgee	O70310.
CleanEx	MM_NMT1.
Genevestigator	O70310.
GermOnline	ENSMUSG00000020936. Mus musculus.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000903. Myristoyl_trans. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 2 hits.
PANTHER	PTHR11377. Myristoyl_trans. 1 hit.
Pfam	PF01233. NMT. 1 hit. PF02799. NMT_C. 1 hit. [Graphical view]
PIRSF	PIRSF015892. N-myristl_transf. 1 hit.
PROSITE	PS00975. NMT_1. 1 hit. PS00976. NMT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	293293.
SOURCE	Search...

Entry information

Entry name

NMT1_MOUSE

Accession

Primary (citable) accession number: O70310

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)