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Protein

Integrin beta-5

Gene

Itgb5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Integrin alpha-V/beta-5 (ITGAV:ITGB5) is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand.

GO - Molecular functioni

  • integrin binding Source: MGI
  • receptor activity Source: InterPro

GO - Biological processi

  • cell-matrix adhesion Source: MGI
  • epithelial cell-cell adhesion Source: MGI
  • integrin-mediated signaling pathway Source: UniProtKB
  • stress fiber assembly Source: UniProtKB
  • transforming growth factor beta receptor signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Integrin, Receptor

Keywords - Biological processi

Cell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin beta-5
Gene namesi
Name:Itgb5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:96614. Itgb5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 719696ExtracellularSequence analysisAdd
BLAST
Transmembranei720 – 74223HelicalSequence analysisAdd
BLAST
Topological domaini743 – 79856CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell leading edge Source: MGI
  • cell surface Source: MGI
  • extracellular exosome Source: MGI
  • focal adhesion Source: MGI
  • integrin alphav-beta5 complex Source: MGI
  • plasma membrane Source: Reactome
  • receptor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 798775Integrin beta-5PRO_0000016349Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi28 ↔ 463By similarity
Disulfide bondi36 ↔ 46By similarity
Disulfide bondi39 ↔ 75By similarity
Disulfide bondi49 ↔ 64By similarity
Disulfide bondi202 ↔ 211By similarity
Disulfide bondi259 ↔ 300By similarity
Glycosylationi347 – 3471N-linked (GlcNAc...)Sequence analysis
Disulfide bondi401 ↔ 413By similarity
Disulfide bondi433 ↔ 682By similarity
Glycosylationi460 – 4601N-linked (GlcNAc...)Sequence analysis
Disulfide bondi461 ↔ 465By similarity
Disulfide bondi476 ↔ 487By similarity
Glycosylationi479 – 4791N-linked (GlcNAc...)Sequence analysis
Disulfide bondi484 ↔ 522By similarity
Disulfide bondi489 ↔ 498By similarity
Disulfide bondi500 ↔ 513By similarity
Glycosylationi505 – 5051N-linked (GlcNAc...)Sequence analysis
Disulfide bondi528 ↔ 533By similarity
Disulfide bondi530 ↔ 563By similarity
Disulfide bondi535 ↔ 548By similarity
Disulfide bondi550 ↔ 555By similarity
Disulfide bondi569 ↔ 574By similarity
Disulfide bondi571 ↔ 602By similarity
Disulfide bondi576 ↔ 585By similarity
Glycosylationi586 – 5861N-linked (GlcNAc...)Sequence analysis
Disulfide bondi587 ↔ 594By similarity
Disulfide bondi608 ↔ 613By similarity
Disulfide bondi610 ↔ 657By similarity
Disulfide bondi615 ↔ 625By similarity
Disulfide bondi628 ↔ 631By similarity
Disulfide bondi635 ↔ 644By similarity
Disulfide bondi641 ↔ 714By similarity
Glycosylationi654 – 6541N-linked (GlcNAc...)Sequence analysis
Disulfide bondi661 ↔ 690By similarity
Glycosylationi705 – 7051N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiO70309.
MaxQBiO70309.
PaxDbiO70309.
PRIDEiO70309.

PTM databases

iPTMnetiO70309.
PhosphoSiteiO70309.

Expressioni

Gene expression databases

CleanExiMM_ITGB5.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. Beta-5 (ITGB5) associates with alpha-V (ITGAV) (Probable). Interacts with MYO10 (By similarity). Interacts with DAB2. Integrin ITGAV:ITGB5 interacts with FBLN5 (via N-terminus) (PubMed:11805835). ITGAV:ITGB5 interacts with NOV (By similarity).By similarity1 Publication2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Myo7aP974793EBI-8401821,EBI-1149557

GO - Molecular functioni

  • integrin binding Source: MGI

Protein-protein interaction databases

BioGridi200832. 2 interactions.
IntActiO70309. 2 interactions.
MINTiMINT-4099048.
STRINGi10090.ENSMUSP00000069416.

Structurei

3D structure databases

ProteinModelPortaliO70309.
SMRiO70309. Positions 24-797.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 7650PSIAdd
BLAST
Domaini136 – 378243VWFAAdd
BLAST
Repeati465 – 51248IAdd
BLAST
Repeati513 – 55442IIAdd
BLAST
Repeati555 – 59339IIIAdd
BLAST
Repeati594 – 63037IVAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni465 – 630166Cysteine-rich tandem repeatsAdd
BLAST

Sequence similaritiesi

Belongs to the integrin beta chain family.Curated
Contains 1 PSI domain.Curated
Contains 1 VWFA domain.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1226. Eukaryota.
ENOG410XP60. LUCA.
HOGENOMiHOG000252936.
HOVERGENiHBG006190.
InParanoidiO70309.
KOiK06588.

Family and domain databases

Gene3Di1.20.5.630. 1 hit.
3.40.50.410. 1 hit.
InterProiIPR027067. Integrin_beta-5.
IPR015812. Integrin_bsu.
IPR014836. Integrin_bsu_cyt_dom.
IPR002369. Integrin_bsu_N.
IPR012896. Integrin_bsu_tail.
IPR032695. Integrin_dom.
IPR016201. Plexin-like_fold.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR10082. PTHR10082. 1 hit.
PTHR10082:SF26. PTHR10082:SF26. 1 hit.
PfamiPF08725. Integrin_b_cyt. 1 hit.
PF07965. Integrin_B_tail. 1 hit.
PF00362. Integrin_beta. 1 hit.
[Graphical view]
PIRSFiPIRSF002512. Integrin_B. 1 hit.
PRINTSiPR01186. INTEGRINB.
SMARTiSM00187. INB. 1 hit.
SM01241. Integrin_b_cyt. 1 hit.
SM01242. Integrin_B_tail. 1 hit.
SM00423. PSI. 1 hit.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF103575. SSF103575. 1 hit.
SSF53300. SSF53300. 1 hit.
SSF69179. SSF69179. 1 hit.
SSF69687. SSF69687. 1 hit.
PROSITEiPS00022. EGF_1. 2 hits.
PS01186. EGF_2. 2 hits.
PS00243. INTEGRIN_BETA. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Beta-5A (identifier: O70309-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPRVPATLYA CLLGLCALVP RLAGLNICTS GSATSCEECL LIHPKCAWCS
60 70 80 90 100
KEYFGNPRSI TSRCDLKANL IRNGCEGEIE SPASSTHVLR NLPLSSKGSS
110 120 130 140 150
ATGSDVIQMT PQEIAVSLRP GEQTTFQLQV RQVEDYPVDL YYLMDLSLSM
160 170 180 190 200
KDDLENIRSL GTKLAEEMRK LTSNFRLGFG SFVDKDISPF SYTAPRYQTN
210 220 230 240 250
PCIGYKLFPN CVPSFGFRHL LPLTDRVDSF NEEVRKQRVS RNRDAPEGGF
260 270 280 290 300
DAVLQAAVCK EKIGWRKDAL HLLVFTTDDV PHIALDGKLG GLVQPHDGQC
310 320 330 340 350
HLNEANEYTA SNQMDYPSLA LLGEKLAENN INLIFAVTKN HYMLYKNFTA
360 370 380 390 400
LIPGTTVEIL HGDSKNIIQL IINAYSSIRA KVELSVWDQP EDLNLFFTAT
410 420 430 440 450
CQDGISYPGQ RKCEGLKIGD TASFEVSVEA RSCPGRQAAQ SFTLRPVGFR
460 470 480 490 500
DSLQVEVAYN CTCGCSTGLE PNSARCSGNG TYTCGLCECD PGYLGTRCEC
510 520 530 540 550
QEGENQSGYQ NLCREAEGKP LCSGRGECSC NQCSCFESEF GRIYGPFCEC
560 570 580 590 600
DSFSCARNKG VLCSGHGECH CGECKCHAGY IGDNCNCSTD VSTCKAKDGQ
610 620 630 640 650
ICSDRGRCVC GQCQCTEPGA FGETCEKCPT CPDACSSKRD CVECLLLHQG
660 670 680 690 700
KPDNQTCHHQ CKDEVITWVD TIVKDDQEAV LCFYKTAKDC VMMFSYTELP
710 720 730 740 750
NGRSNLTVLR EPECGSAPNA MTILLAVVGS ILLIGMALLA IWKLLVTIHD
760 770 780 790
RREFAKFQSE RSRARYEMAS NPLYRKPIST HTVDFAFNKF NKSYNGSV
Length:798
Mass (Da):87,909
Last modified:April 27, 2001 - v2
Checksum:i34B9D8B07E2688B0
GO
Isoform Beta-5B (identifier: O70309-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     760-798: ERSRARYEMA...KFNKSYNGSV → LKPPVQKAHL...PWTSWTICSR

Show »
Length:816
Mass (Da):90,035
Checksum:i8ED736C097EBA855
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti96 – 961S → C in AAD08782 (PubMed:9880508).Curated
Sequence conflicti259 – 2591C → Y in AAD08782 (PubMed:9880508).Curated
Sequence conflicti595 – 5951K → R in AAD08782 (PubMed:9880508).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei760 – 79839ERSRA…YNGSV → LKPPVQKAHLHTHCRFRLQQ VQQILQWLSGLRLLDGWRGT KDEDSGVPWTSWTICSR in isoform Beta-5B. 1 PublicationVSP_002752Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043257 mRNA. Translation: AAC40110.1.
AF043256 mRNA. Translation: AAC40109.1.
AF022110 mRNA. Translation: AAD08782.1.
CCDSiCCDS28135.1. [O70309-2]
RefSeqiNP_001139356.1. NM_001145884.1.
NP_034710.2. NM_010580.2.
UniGeneiMm.6424.

Genome annotation databases

GeneIDi16419.
KEGGimmu:16419.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043257 mRNA. Translation: AAC40110.1.
AF043256 mRNA. Translation: AAC40109.1.
AF022110 mRNA. Translation: AAD08782.1.
CCDSiCCDS28135.1. [O70309-2]
RefSeqiNP_001139356.1. NM_001145884.1.
NP_034710.2. NM_010580.2.
UniGeneiMm.6424.

3D structure databases

ProteinModelPortaliO70309.
SMRiO70309. Positions 24-797.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200832. 2 interactions.
IntActiO70309. 2 interactions.
MINTiMINT-4099048.
STRINGi10090.ENSMUSP00000069416.

PTM databases

iPTMnetiO70309.
PhosphoSiteiO70309.

Proteomic databases

EPDiO70309.
MaxQBiO70309.
PaxDbiO70309.
PRIDEiO70309.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi16419.
KEGGimmu:16419.

Organism-specific databases

CTDi3693.
MGIiMGI:96614. Itgb5.

Phylogenomic databases

eggNOGiKOG1226. Eukaryota.
ENOG410XP60. LUCA.
HOGENOMiHOG000252936.
HOVERGENiHBG006190.
InParanoidiO70309.
KOiK06588.

Miscellaneous databases

ChiTaRSiItgb5. mouse.
PROiO70309.
SOURCEiSearch...

Gene expression databases

CleanExiMM_ITGB5.

Family and domain databases

Gene3Di1.20.5.630. 1 hit.
3.40.50.410. 1 hit.
InterProiIPR027067. Integrin_beta-5.
IPR015812. Integrin_bsu.
IPR014836. Integrin_bsu_cyt_dom.
IPR002369. Integrin_bsu_N.
IPR012896. Integrin_bsu_tail.
IPR032695. Integrin_dom.
IPR016201. Plexin-like_fold.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR10082. PTHR10082. 1 hit.
PTHR10082:SF26. PTHR10082:SF26. 1 hit.
PfamiPF08725. Integrin_b_cyt. 1 hit.
PF07965. Integrin_B_tail. 1 hit.
PF00362. Integrin_beta. 1 hit.
[Graphical view]
PIRSFiPIRSF002512. Integrin_B. 1 hit.
PRINTSiPR01186. INTEGRINB.
SMARTiSM00187. INB. 1 hit.
SM01241. Integrin_b_cyt. 1 hit.
SM01242. Integrin_B_tail. 1 hit.
SM00423. PSI. 1 hit.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF103575. SSF103575. 1 hit.
SSF53300. SSF53300. 1 hit.
SSF69179. SSF69179. 1 hit.
SSF69687. SSF69687. 1 hit.
PROSITEiPS00022. EGF_1. 2 hits.
PS01186. EGF_2. 2 hits.
PS00243. INTEGRIN_BETA. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning reveals two mouse beta5 integrin transcripts distinct in cytoplasmic domains as a result of alternative splicing."
    Zhang H., Tan S.M., Lu J.
    Biochem. J. 331:631-637(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS BETA-5A AND BETA-5B).
    Tissue: Liver.
  2. "Cloning of the murine beta5 integrin subunit promoter. Identification of a novel sequence mediating granulocyte-macrophage colony-stimulating factor-dependent repression of beta5 integrin gene transcription."
    Feng X., Teitelbaum S.L., Quiroz M.E., Towler D.A., Ross F.P.
    J. Biol. Chem. 274:1366-1374(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA-5A).
    Tissue: Brain.
  3. Cited for: INTERACTION WITH FBLN5.
  4. "Integrin beta cytoplasmic domain interactions with phosphotyrosine-binding domains: a structural prototype for diversity in integrin signaling."
    Calderwood D.A., Fujioka Y., de Pereda J.M., Garcia-Alvarez B., Nakamoto T., Margolis B., McGlade C.J., Liddington R.C., Ginsberg M.H.
    Proc. Natl. Acad. Sci. U.S.A. 100:2272-2277(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DAB2.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney, Lung and Testis.

Entry informationi

Entry nameiITB5_MOUSE
AccessioniPrimary (citable) accession number: O70309
Secondary accession number(s): O70308, O88347
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: June 8, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.