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O70302 (CIDEA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cell death activator CIDE-A
Alternative name(s):
Cell death-inducing DFFA-like effector A
Gene names
Name:Cidea
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length217 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair and occurs at a lower rate than that promoted by CIDEC. Acts as a CEBPB coactivator in mammary epithelial cells to control the expression of a subset of CEBPB downstream target genes, including ID2, IGF1, PRLR, SOCS1, SOCS3, XDH, but not casein. By interacting with CEBPB, strengthens the association of CEBPB with the XDH promoter, increases histone acetylation and dissociates HDAC1 from the promoter. When overexpressed, induces apoptosis. The physiological significance of its role in apoptosis is unclear. Ref.1 Ref.6 Ref.7 Ref.8

Subunit structure

Interacts with CIDEC By similarity. Directly interacts with CEBPB. Ref.8

Subcellular location

Lipid droplet. Nucleus. Note: Enriched at lipid droplet contact sites. Using a GFP-tagged construct, has been shown to localize to mitonchondria, where it could interact with UCP1 and hence inhibit UCP1 uncoupling activity (Ref.5). These data could not be confirmed (Ref.8, Ref.6). Ref.5 Ref.6 Ref.7 Ref.8

Tissue specificity

Highly expressed in brown adipose tissue and, at lower levels, in white adipose tissue (at protein level). Expressed in mammary gland during pregnancy and lactation, in epithelial cells, but not in the surrounding adipose tissue. Secreted into milk via milk fat globules. Undetectable in undifferentiated preadipocytes. Ref.5 Ref.6 Ref.8

Developmental stage

Expressed at 15 dpc in the interscapular region of the embryo, that could correspond to the developing brown adipose tissue. Expression continues in the interscapular region at 18 dpc and postnatally. In mammary glands, begins to be highly expressed at day 14.5 of pregnancy. Expression is maintained at high levels throughout lactation and declines during post-lactational involution. Ref.8

Induction

Up-regulated under conditions that enhance triacylglycerol deposition, including rosiglitazone treatment and high-fat diet. This up-regulation is mediated by PPARG. Ref.6

Disruption phenotype

Mutant animals appear normal and fertile and produce the expected Mendelian ratio of heterozygous and homozygous descendents. They are lean and resistant to diet-induced obesity and diabetes. They exhibit higher metabolic rate, lipolysis in brown adipose tissue and core body temperature when subjected to cold treatment. Mutant females are unable to properly feed their pups who die within 3 days postpartum due to severely reduced milk lipids. Ref.5 Ref.8

Sequence similarities

Contains 1 CIDE-N domain.

Ontologies

Keywords
   Biological processApoptosis
Transcription
Transcription regulation
   Cellular componentLipid droplet
Nucleus
   Molecular functionActivator
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processapoptotic process

Inferred from electronic annotation. Source: UniProtKB-KW

cell death

Inferred from direct assay PubMed 17080483. Source: BHF-UCL

lipid metabolic process

Inferred from mutant phenotype Ref.5. Source: MGI

lipid storage

Inferred from mutant phenotype Ref.5. Source: BHF-UCL

negative regulation of cytokine secretion

Inferred from electronic annotation. Source: Ensembl

negative regulation of execution phase of apoptosis

Inferred from direct assay PubMed 17080483. Source: BHF-UCL

negative regulation of lipid catabolic process

Inferred from mutant phenotype Ref.5. Source: BHF-UCL

negative regulation of transforming growth factor beta receptor signaling pathway

Inferred from direct assay PubMed 17080483. Source: BHF-UCL

negative regulation of tumor necrosis factor production

Inferred from electronic annotation. Source: Ensembl

positive regulation of sequestering of triglyceride

Inferred from direct assay Ref.6. Source: BHF-UCL

regulation of apoptotic DNA fragmentation

Inferred from direct assay Ref.1. Source: BHF-UCL

regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

response to stilbenoid

Inferred from expression pattern PubMed 17086191. Source: UniProtKB

temperature homeostasis

Inferred from mutant phenotype Ref.5. Source: BHF-UCL

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 17080483. Source: BHF-UCL

lipid particle

Inferred from electronic annotation. Source: UniProtKB-SubCell

mitochondrial envelope

Inferred from direct assay Ref.5. Source: MGI

mitochondrion

Inferred from direct assay Ref.5. Source: MGI

nucleus

Inferred from direct assay PubMed 17080483. Source: BHF-UCL

   Molecular_functionprotein homodimerization activity

Inferred from direct assay PubMed 17080483. Source: BHF-UCL

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PRKAB1Q9Y4784EBI-7927848,EBI-719769From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 217217Cell death activator CIDE-A
PRO_0000144719

Regions

Domain33 – 11078CIDE-N

Experimental info

Mutagenesis231K → A: Sustantial reduction in nuclear localization and loss of XDH induction; when associated with A-24. Ref.8
Mutagenesis231K → R: Increased nuclear localization. Ref.8
Mutagenesis241K → A: Sustantial reduction in nuclear localization and loss of XDH induction; when associated with A-23. Ref.8
Sequence conflict1651S → R in AAC34985. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O70302 [UniParc].

Last modified October 3, 2012. Version 2.
Checksum: C9E406895CFD4CBA

FASTA21724,669
        10         20         30         40         50         60 
METARDYAGA LIRPLTFMGL QTKKVLLTPL IHPARPFRVS NHDRSSRRGV MASSLQELIS 

        70         80         90        100        110        120 
KTLDVLVITT GLVTLVLEED GTVVDTEEFF QTLRDNTHFM ILEKGQKWTP GSKYVPVCKQ 

       130        140        150        160        170        180 
PKKSGIARVT FDLYRLNPKD FLGCLNVKAT MYEMYSVSYD IRCTSFKAVL RNLLRFMSYA 

       190        200        210 
AQMTGQFLVY AGTYMLRVLG DTEEQPSPKP STKGWFM 

« Hide

References

« Hide 'large scale' references
[1]"CIDE, a novel family of cell death activators with homology to the 45 kDa subunit of the DNA fragmentation factor."
Inohara N., Koseki T., Chen S., Wu X., Nunez G.
EMBO J. 17:2526-2533(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION IN APOPTOSIS.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Mammary gland.
[5]"Cidea-deficient mice have lean phenotype and are resistant to obesity."
Zhou Z., Yon Toh S., Chen Z., Guo K., Ng C.P., Ponniah S., Lin S.C., Hong W., Li P.
Nat. Genet. 35:49-56(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[6]"Cidea is associated with lipid droplets and insulin sensitivity in humans."
Puri V., Ranjit S., Konda S., Nicoloro S.M., Straubhaar J., Chawla A., Chouinard M., Lin C., Burkart A., Corvera S., Perugini R.A., Czech M.P.
Proc. Natl. Acad. Sci. U.S.A. 105:7833-7838(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[7]"Fsp27 promotes lipid droplet growth by lipid exchange and transfer at lipid droplet contact sites."
Gong J., Sun Z., Wu L., Xu W., Schieber N., Xu D., Shui G., Yang H., Parton R.G., Li P.
J. Cell Biol. 195:953-963(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]"Cidea is an essential transcriptional coactivator regulating mammary gland secretion of milk lipids."
Wang W., Lv N., Zhang S., Shui G., Qian H., Zhang J., Chen Y., Ye J., Xie Y., Shen Y., Wenk M.R., Li P.
Nat. Med. 18:235-243(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A CEBPB COACTIVATOR, INTERACTION WITH CEBPB, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, MUTAGENESIS OF LYS-23 AND LYS-24.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF041376 mRNA. Translation: AAC34985.1.
AC154125 Genomic DNA. No translation available.
CH466528 Genomic DNA. Translation: EDL09649.1.
CH466528 Genomic DNA. Translation: EDL09650.1.
BC096649 mRNA. Translation: AAH96649.1.
RefSeqNP_031728.2. NM_007702.2.
UniGeneMm.449.

3D structure databases

ProteinModelPortalO70302.
SMRO70302. Positions 33-110.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO70302. 7 interactions.
STRING10090.ENSMUSP00000025404.

PTM databases

PhosphoSiteO70302.

Proteomic databases

PaxDbO70302.
PRIDEO70302.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000025404; ENSMUSP00000025404; ENSMUSG00000024526.
GeneID12683.
KEGGmmu:12683.
UCSCuc012beh.1. mouse.

Organism-specific databases

CTD1149.
MGIMGI:1270845. Cidea.

Phylogenomic databases

eggNOGNOG43753.
GeneTreeENSGT00390000018596.
HOGENOMHOG000029211.
HOVERGENHBG050961.
InParanoidO70302.
OMAYRLNPKD.
OrthoDBEOG7K3TN6.
TreeFamTF334321.

Gene expression databases

GenevestigatorO70302.

Family and domain databases

InterProIPR003508. CIDE-N_dom.
[Graphical view]
PfamPF02017. CIDE-N. 1 hit.
[Graphical view]
SMARTSM00266. CAD. 1 hit.
[Graphical view]
PROSITEPS51135. CIDE_N. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio281928.
PROO70302.
SOURCESearch...

Entry information

Entry nameCIDEA_MOUSE
AccessionPrimary (citable) accession number: O70302
Secondary accession number(s): Q4V9X2
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: October 3, 2012
Last modified: April 16, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot