Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O70302

- CIDEA_MOUSE

UniProt

O70302 - CIDEA_MOUSE

Protein

Cell death activator CIDE-A

Gene

Cidea

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 2 (03 Oct 2012)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair and occurs at a lower rate than that promoted by CIDEC. Acts as a CEBPB coactivator in mammary epithelial cells to control the expression of a subset of CEBPB downstream target genes, including ID2, IGF1, PRLR, SOCS1, SOCS3, XDH, but not casein. By interacting with CEBPB, strengthens the association of CEBPB with the XDH promoter, increases histone acetylation and dissociates HDAC1 from the promoter. When overexpressed, induces apoptosis. The physiological significance of its role in apoptosis is unclear.4 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein homodimerization activity Source: BHF-UCL

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW
    2. cell death Source: BHF-UCL
    3. lipid metabolic process Source: MGI
    4. lipid storage Source: BHF-UCL
    5. negative regulation of cytokine secretion Source: Ensembl
    6. negative regulation of execution phase of apoptosis Source: BHF-UCL
    7. negative regulation of lipid catabolic process Source: BHF-UCL
    8. negative regulation of transforming growth factor beta receptor signaling pathway Source: BHF-UCL
    9. negative regulation of tumor necrosis factor production Source: Ensembl
    10. positive regulation of sequestering of triglyceride Source: BHF-UCL
    11. regulation of apoptotic DNA fragmentation Source: BHF-UCL
    12. regulation of transcription, DNA-templated Source: UniProtKB-KW
    13. response to stilbenoid Source: UniProtKB
    14. temperature homeostasis Source: BHF-UCL
    15. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Apoptosis, Transcription, Transcription regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cell death activator CIDE-A
    Alternative name(s):
    Cell death-inducing DFFA-like effector A
    Gene namesi
    Name:Cidea
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 18

    Organism-specific databases

    MGIiMGI:1270845. Cidea.

    Subcellular locationi

    Lipid droplet. Nucleus
    Note: Enriched at lipid droplet contact sites. Using a GFP-tagged construct, has been shown to localize to mitonchondria, where it could interact with UCP1 and hence inhibit UCP1 uncoupling activity (PubMed:12910269). These data could not be confirmed (PubMed:22245780, PubMed:18509062).3 Publications

    GO - Cellular componenti

    1. cytoplasm Source: BHF-UCL
    2. lipid particle Source: UniProtKB-SubCell
    3. mitochondrial envelope Source: MGI
    4. mitochondrion Source: MGI
    5. nucleus Source: BHF-UCL

    Keywords - Cellular componenti

    Lipid droplet, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Mutant animals appear normal and fertile and produce the expected Mendelian ratio of heterozygous and homozygous descendents. They are lean and resistant to diet-induced obesity and diabetes. They exhibit higher metabolic rate, lipolysis in brown adipose tissue and core body temperature when subjected to cold treatment. Mutant females are unable to properly feed their pups who die within 3 days postpartum due to severely reduced milk lipids.2 Publications

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi23 – 231K → A: Sustantial reduction in nuclear localization and loss of XDH induction; when associated with A-24. 1 Publication
    Mutagenesisi23 – 231K → R: Increased nuclear localization. 1 Publication
    Mutagenesisi24 – 241K → A: Sustantial reduction in nuclear localization and loss of XDH induction; when associated with A-23. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 217217Cell death activator CIDE-APRO_0000144719Add
    BLAST

    Proteomic databases

    PaxDbiO70302.
    PRIDEiO70302.

    PTM databases

    PhosphoSiteiO70302.

    Expressioni

    Tissue specificityi

    Highly expressed in brown adipose tissue and, at lower levels, in white adipose tissue (at protein level). Expressed in mammary gland during pregnancy and lactation, in epithelial cells, but not in the surrounding adipose tissue. Secreted into milk via milk fat globules. Undetectable in undifferentiated preadipocytes.3 Publications

    Developmental stagei

    Expressed at 15 dpc in the interscapular region of the embryo, that could correspond to the developing brown adipose tissue. Expression continues in the interscapular region at 18 dpc and postnatally. In mammary glands, begins to be highly expressed at day 14.5 of pregnancy. Expression is maintained at high levels throughout lactation and declines during post-lactational involution.1 Publication

    Inductioni

    Up-regulated under conditions that enhance triacylglycerol deposition, including rosiglitazone treatment and high-fat diet. This up-regulation is mediated by PPARG.1 Publication

    Gene expression databases

    GenevestigatoriO70302.

    Interactioni

    Subunit structurei

    Interacts with CIDEC By similarity. Directly interacts with CEBPB.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PRKAB1Q9Y4784EBI-7927848,EBI-719769From a different organism.

    Protein-protein interaction databases

    IntActiO70302. 7 interactions.
    STRINGi10090.ENSMUSP00000025404.

    Structurei

    3D structure databases

    ProteinModelPortaliO70302.
    SMRiO70302. Positions 33-110.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini33 – 11078CIDE-NPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 CIDE-N domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG43753.
    GeneTreeiENSGT00390000018596.
    HOGENOMiHOG000029211.
    HOVERGENiHBG050961.
    InParanoidiO70302.
    OMAiAQITGQF.
    OrthoDBiEOG7K3TN6.
    TreeFamiTF334321.

    Family and domain databases

    InterProiIPR003508. CIDE-N_dom.
    [Graphical view]
    PfamiPF02017. CIDE-N. 1 hit.
    [Graphical view]
    SMARTiSM00266. CAD. 1 hit.
    [Graphical view]
    PROSITEiPS51135. CIDE_N. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O70302-1 [UniParc]FASTAAdd to Basket

    « Hide

    METARDYAGA LIRPLTFMGL QTKKVLLTPL IHPARPFRVS NHDRSSRRGV    50
    MASSLQELIS KTLDVLVITT GLVTLVLEED GTVVDTEEFF QTLRDNTHFM 100
    ILEKGQKWTP GSKYVPVCKQ PKKSGIARVT FDLYRLNPKD FLGCLNVKAT 150
    MYEMYSVSYD IRCTSFKAVL RNLLRFMSYA AQMTGQFLVY AGTYMLRVLG 200
    DTEEQPSPKP STKGWFM 217
    Length:217
    Mass (Da):24,669
    Last modified:October 3, 2012 - v2
    Checksum:iC9E406895CFD4CBA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti165 – 1651S → R in AAC34985. (PubMed:9564035)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF041376 mRNA. Translation: AAC34985.1.
    AC154125 Genomic DNA. No translation available.
    CH466528 Genomic DNA. Translation: EDL09649.1.
    CH466528 Genomic DNA. Translation: EDL09650.1.
    BC096649 mRNA. Translation: AAH96649.1.
    CCDSiCCDS37846.1.
    RefSeqiNP_031728.2. NM_007702.2.
    UniGeneiMm.449.

    Genome annotation databases

    EnsembliENSMUST00000025404; ENSMUSP00000025404; ENSMUSG00000024526.
    GeneIDi12683.
    KEGGimmu:12683.
    UCSCiuc012beh.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF041376 mRNA. Translation: AAC34985.1 .
    AC154125 Genomic DNA. No translation available.
    CH466528 Genomic DNA. Translation: EDL09649.1 .
    CH466528 Genomic DNA. Translation: EDL09650.1 .
    BC096649 mRNA. Translation: AAH96649.1 .
    CCDSi CCDS37846.1.
    RefSeqi NP_031728.2. NM_007702.2.
    UniGenei Mm.449.

    3D structure databases

    ProteinModelPortali O70302.
    SMRi O70302. Positions 33-110.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O70302. 7 interactions.
    STRINGi 10090.ENSMUSP00000025404.

    PTM databases

    PhosphoSitei O70302.

    Proteomic databases

    PaxDbi O70302.
    PRIDEi O70302.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000025404 ; ENSMUSP00000025404 ; ENSMUSG00000024526 .
    GeneIDi 12683.
    KEGGi mmu:12683.
    UCSCi uc012beh.1. mouse.

    Organism-specific databases

    CTDi 1149.
    MGIi MGI:1270845. Cidea.

    Phylogenomic databases

    eggNOGi NOG43753.
    GeneTreei ENSGT00390000018596.
    HOGENOMi HOG000029211.
    HOVERGENi HBG050961.
    InParanoidi O70302.
    OMAi AQITGQF.
    OrthoDBi EOG7K3TN6.
    TreeFami TF334321.

    Miscellaneous databases

    NextBioi 281928.
    PROi O70302.
    SOURCEi Search...

    Gene expression databases

    Genevestigatori O70302.

    Family and domain databases

    InterProi IPR003508. CIDE-N_dom.
    [Graphical view ]
    Pfami PF02017. CIDE-N. 1 hit.
    [Graphical view ]
    SMARTi SM00266. CAD. 1 hit.
    [Graphical view ]
    PROSITEi PS51135. CIDE_N. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "CIDE, a novel family of cell death activators with homology to the 45 kDa subunit of the DNA fragmentation factor."
      Inohara N., Koseki T., Chen S., Wu X., Nunez G.
      EMBO J. 17:2526-2533(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION IN APOPTOSIS.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Mammary gland.
    5. "Cidea-deficient mice have lean phenotype and are resistant to obesity."
      Zhou Z., Yon Toh S., Chen Z., Guo K., Ng C.P., Ponniah S., Lin S.C., Hong W., Li P.
      Nat. Genet. 35:49-56(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    6. Cited for: FUNCTION, INDUCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    7. "Fsp27 promotes lipid droplet growth by lipid exchange and transfer at lipid droplet contact sites."
      Gong J., Sun Z., Wu L., Xu W., Schieber N., Xu D., Shui G., Yang H., Parton R.G., Li P.
      J. Cell Biol. 195:953-963(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    8. "Cidea is an essential transcriptional coactivator regulating mammary gland secretion of milk lipids."
      Wang W., Lv N., Zhang S., Shui G., Qian H., Zhang J., Chen Y., Ye J., Xie Y., Shen Y., Wenk M.R., Li P.
      Nat. Med. 18:235-243(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A CEBPB COACTIVATOR, INTERACTION WITH CEBPB, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, MUTAGENESIS OF LYS-23 AND LYS-24.

    Entry informationi

    Entry nameiCIDEA_MOUSE
    AccessioniPrimary (citable) accession number: O70302
    Secondary accession number(s): Q4V9X2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 90 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3