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O70291

- GRK4_MOUSE

UniProt

O70291 - GRK4_MOUSE

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Protein

G protein-coupled receptor kinase 4

Gene
Grk4, Gprk2l
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Specifically phosphorylates the activated forms of G protein-coupled receptors.

Catalytic activityi

ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.

Enzyme regulationi

Inhibited by heparin By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei215 – 2151ATP By similarity
Active sitei311 – 3111Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi192 – 2009ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. G-protein coupled receptor kinase activity Source: UniProtKB-EC
  3. rhodopsin kinase activity Source: Ensembl

GO - Biological processi

  1. G-protein coupled receptor internalization Source: Ensembl
  2. signal transduction Source: InterPro
  3. termination of G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
G protein-coupled receptor kinase 4 (EC:2.7.11.16)
Alternative name(s):
G protein-coupled receptor kinase GRK4
Gene namesi
Name:Grk4
Synonyms:Gprk2l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:95801. Grk4.

Subcellular locationi

Cytoplasm. Cytoplasmcell cortex By similarity

GO - Cellular componenti

  1. cell cortex Source: UniProtKB-SubCell
  2. cytosol Source: Ensembl
  3. dendrite Source: Ensembl
  4. neuronal cell body Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 574574G protein-coupled receptor kinase 4PRO_0000085968Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity

Post-translational modificationi

Palmitoylated By similarity.

Keywords - PTMi

Acetylation, Lipoprotein, Palmitate

Proteomic databases

PRIDEiO70291.

PTM databases

PhosphoSiteiO70291.

Expressioni

Gene expression databases

ArrayExpressiO70291.
BgeeiO70291.
CleanExiMM_GRK4.
GenevestigatoriO70291.

Interactioni

Subunit structurei

Interacts with DRD3 By similarity.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000001112.

Structurei

3D structure databases

ProteinModelPortaliO70291.
SMRiO70291. Positions 2-525.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini51 – 171121RGSAdd
BLAST
Domaini186 – 448263Protein kinaseAdd
BLAST
Domaini449 – 51466AGC-kinase C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 153153N-terminalAdd
BLAST

Sequence similaritiesi

Contains 1 RGS domain.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00730000110512.
HOGENOMiHOG000006742.
HOVERGENiHBG004532.
InParanoidiQ3V151.
KOiK08291.
OMAiHSIEKDY.
OrthoDBiEOG7V1FQK.
TreeFamiTF313940.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR00717. GPCRKINASE.
SMARTiSM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O70291-1 [UniParc]FASTAAdd to Basket

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MELENFVANN LLLKARLGFN KQTGRSKKWR ELLKFPPVSM CTELRWSIEK    50
DFSSLCDKQP IGRLLFRQFC DTKPDLKRCI EFLDAVAEYE VTIEEEQREF 100
GLAIFSRFFK EKSEVPLPEI PPDIVKECKW NLKQNSPSQN VFEECAGIVC 150
KYLSETPFEE YQESTYFNRF LQWKWLERRP VTKNTFRQYR VLGKGGFGEV 200
CACQVRATGK MYACKKLEKK RIKKRKGEAM ALNEKRILEK LHSRFVVSLA 250
YTYETKDALC LVLTIMNGGD LKYHIYNLGD PGFEEPRAVF YAAELCCGLE 300
DLQRKRIVYR DLKPENILLD DHGHIRISDL GLAMEVPEGE MVRGRVGTVG 350
YMAPEIINHE KYTFSPDWWG LGCLIYEMIA GHSPFRKYKE KVNREELERR 400
VKNETEEYSE RFSEDAKSIC SMLLIKDPSK RLGCQRDGVS AVKQHPIFKD 450
INFSRLEANM LDPPFIPDPQ AIYCRNILDI GQFSVVKGVN LDTNDEIFYA 500
EFATGSVTIP WQNEMIESGC FKDLNENEDD LSSLEKYKMC SSILRPKRNF 550
FRRLFRRTGC LNIALSEERE PTEH 574
Length:574
Mass (Da):66,928
Last modified:July 27, 2011 - v2
Checksum:i7D7D6F46C9C044B6
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 61F → M in AAC09266. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF040745 mRNA. Translation: AAC09266.1.
AK132685 mRNA. Translation: BAE21301.1.
CH466524 Genomic DNA. Translation: EDL37466.1.
BC150691 mRNA. Translation: AAI50692.1.
CCDSiCCDS19219.1.
RefSeqiNP_001074212.1. NM_001080743.1.
NP_062370.2. NM_019497.2.
UniGeneiMm.117076.

Genome annotation databases

EnsembliENSMUST00000001112; ENSMUSP00000001112; ENSMUSG00000052783.
GeneIDi14772.
KEGGimmu:14772.
UCSCiuc008xcz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF040745 mRNA. Translation: AAC09266.1 .
AK132685 mRNA. Translation: BAE21301.1 .
CH466524 Genomic DNA. Translation: EDL37466.1 .
BC150691 mRNA. Translation: AAI50692.1 .
CCDSi CCDS19219.1.
RefSeqi NP_001074212.1. NM_001080743.1.
NP_062370.2. NM_019497.2.
UniGenei Mm.117076.

3D structure databases

ProteinModelPortali O70291.
SMRi O70291. Positions 2-525.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000001112.

PTM databases

PhosphoSitei O70291.

Proteomic databases

PRIDEi O70291.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000001112 ; ENSMUSP00000001112 ; ENSMUSG00000052783 .
GeneIDi 14772.
KEGGi mmu:14772.
UCSCi uc008xcz.1. mouse.

Organism-specific databases

CTDi 2868.
MGIi MGI:95801. Grk4.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00730000110512.
HOGENOMi HOG000006742.
HOVERGENi HBG004532.
InParanoidi Q3V151.
KOi K08291.
OMAi HSIEKDY.
OrthoDBi EOG7V1FQK.
TreeFami TF313940.

Miscellaneous databases

NextBioi 286867.
PROi O70291.
SOURCEi Search...

Gene expression databases

ArrayExpressi O70291.
Bgeei O70291.
CleanExi MM_GRK4.
Genevestigatori O70291.

Family and domain databases

InterProi IPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view ]
PRINTSi PR00717. GPCRKINASE.
SMARTi SM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The GRK4 subfamily of G protein-coupled receptor kinases. Alternative splicing, gene organization, and sequence conservation."
    Premont R.T., Macrae A.D., Aparicio S.A., Kendall H.E., Welch J.E., Lefkowitz R.J.
    J. Biol. Chem. 274:29381-29389(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Testis.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiGRK4_MOUSE
AccessioniPrimary (citable) accession number: O70291
Secondary accession number(s): Q3V151
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3