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O70291

- GRK4_MOUSE

UniProt

O70291 - GRK4_MOUSE

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Protein

G protein-coupled receptor kinase 4

Gene

Grk4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Specifically phosphorylates the activated forms of G protein-coupled receptors.

Catalytic activityi

ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.

Enzyme regulationi

Inhibited by heparin.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei215 – 2151ATPPROSITE-ProRule annotation
Active sitei311 – 3111Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi192 – 2009ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. G-protein coupled receptor kinase activity Source: UniProtKB-EC
  3. rhodopsin kinase activity Source: Ensembl

GO - Biological processi

  1. G-protein coupled receptor internalization Source: Ensembl
  2. signal transduction Source: InterPro
  3. termination of G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
G protein-coupled receptor kinase 4 (EC:2.7.11.16)
Alternative name(s):
G protein-coupled receptor kinase GRK4
Gene namesi
Name:Grk4
Synonyms:Gprk2l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:95801. Grk4.

Subcellular locationi

Cytoplasm. Cytoplasmcell cortex By similarity

GO - Cellular componenti

  1. cytosol Source: Ensembl
  2. dendrite Source: Ensembl
  3. neuronal cell body Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 574574G protein-coupled receptor kinase 4PRO_0000085968Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Post-translational modificationi

Palmitoylated.By similarity

Keywords - PTMi

Acetylation, Lipoprotein, Palmitate

Proteomic databases

PRIDEiO70291.

PTM databases

PhosphoSiteiO70291.

Expressioni

Gene expression databases

BgeeiO70291.
CleanExiMM_GRK4.
ExpressionAtlasiO70291. baseline and differential.
GenevestigatoriO70291.

Interactioni

Subunit structurei

Interacts with DRD3.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000001112.

Structurei

3D structure databases

ProteinModelPortaliO70291.
SMRiO70291. Positions 2-525.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini51 – 171121RGSPROSITE-ProRule annotationAdd
BLAST
Domaini186 – 448263Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini449 – 51466AGC-kinase C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 153153N-terminalAdd
BLAST

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 RGS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00770000120493.
HOGENOMiHOG000006742.
HOVERGENiHBG004532.
InParanoidiO70291.
KOiK08291.
OMAiHSIEKDY.
OrthoDBiEOG7V1FQK.
TreeFamiTF313940.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR00717. GPCRKINASE.
SMARTiSM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O70291-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MELENFVANN LLLKARLGFN KQTGRSKKWR ELLKFPPVSM CTELRWSIEK
60 70 80 90 100
DFSSLCDKQP IGRLLFRQFC DTKPDLKRCI EFLDAVAEYE VTIEEEQREF
110 120 130 140 150
GLAIFSRFFK EKSEVPLPEI PPDIVKECKW NLKQNSPSQN VFEECAGIVC
160 170 180 190 200
KYLSETPFEE YQESTYFNRF LQWKWLERRP VTKNTFRQYR VLGKGGFGEV
210 220 230 240 250
CACQVRATGK MYACKKLEKK RIKKRKGEAM ALNEKRILEK LHSRFVVSLA
260 270 280 290 300
YTYETKDALC LVLTIMNGGD LKYHIYNLGD PGFEEPRAVF YAAELCCGLE
310 320 330 340 350
DLQRKRIVYR DLKPENILLD DHGHIRISDL GLAMEVPEGE MVRGRVGTVG
360 370 380 390 400
YMAPEIINHE KYTFSPDWWG LGCLIYEMIA GHSPFRKYKE KVNREELERR
410 420 430 440 450
VKNETEEYSE RFSEDAKSIC SMLLIKDPSK RLGCQRDGVS AVKQHPIFKD
460 470 480 490 500
INFSRLEANM LDPPFIPDPQ AIYCRNILDI GQFSVVKGVN LDTNDEIFYA
510 520 530 540 550
EFATGSVTIP WQNEMIESGC FKDLNENEDD LSSLEKYKMC SSILRPKRNF
560 570
FRRLFRRTGC LNIALSEERE PTEH
Length:574
Mass (Da):66,928
Last modified:July 27, 2011 - v2
Checksum:i7D7D6F46C9C044B6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 61F → M in AAC09266. (PubMed:10506199)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF040745 mRNA. Translation: AAC09266.1.
AK132685 mRNA. Translation: BAE21301.1.
CH466524 Genomic DNA. Translation: EDL37466.1.
BC150691 mRNA. Translation: AAI50692.1.
CCDSiCCDS19219.1.
RefSeqiNP_001074212.1. NM_001080743.1.
NP_062370.2. NM_019497.2.
UniGeneiMm.117076.

Genome annotation databases

EnsembliENSMUST00000001112; ENSMUSP00000001112; ENSMUSG00000052783.
GeneIDi14772.
KEGGimmu:14772.
UCSCiuc008xcz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF040745 mRNA. Translation: AAC09266.1 .
AK132685 mRNA. Translation: BAE21301.1 .
CH466524 Genomic DNA. Translation: EDL37466.1 .
BC150691 mRNA. Translation: AAI50692.1 .
CCDSi CCDS19219.1.
RefSeqi NP_001074212.1. NM_001080743.1.
NP_062370.2. NM_019497.2.
UniGenei Mm.117076.

3D structure databases

ProteinModelPortali O70291.
SMRi O70291. Positions 2-525.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000001112.

PTM databases

PhosphoSitei O70291.

Proteomic databases

PRIDEi O70291.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000001112 ; ENSMUSP00000001112 ; ENSMUSG00000052783 .
GeneIDi 14772.
KEGGi mmu:14772.
UCSCi uc008xcz.1. mouse.

Organism-specific databases

CTDi 2868.
MGIi MGI:95801. Grk4.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00770000120493.
HOGENOMi HOG000006742.
HOVERGENi HBG004532.
InParanoidi O70291.
KOi K08291.
OMAi HSIEKDY.
OrthoDBi EOG7V1FQK.
TreeFami TF313940.

Miscellaneous databases

NextBioi 286867.
PROi O70291.
SOURCEi Search...

Gene expression databases

Bgeei O70291.
CleanExi MM_GRK4.
ExpressionAtlasi O70291. baseline and differential.
Genevestigatori O70291.

Family and domain databases

InterProi IPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view ]
PRINTSi PR00717. GPCRKINASE.
SMARTi SM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The GRK4 subfamily of G protein-coupled receptor kinases. Alternative splicing, gene organization, and sequence conservation."
    Premont R.T., Macrae A.D., Aparicio S.A., Kendall H.E., Welch J.E., Lefkowitz R.J.
    J. Biol. Chem. 274:29381-29389(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Testis.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiGRK4_MOUSE
AccessioniPrimary (citable) accession number: O70291
Secondary accession number(s): Q3V151
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3