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Protein

G protein-coupled receptor kinase 4

Gene

Grk4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Specifically phosphorylates the activated forms of G protein-coupled receptors.

Catalytic activityi

ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.

Enzyme regulationi

Inhibited by heparin.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei215ATPPROSITE-ProRule annotation1
Active sitei311Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi192 – 200ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2514859. Inactivation, recovery and regulation of the phototransduction cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
G protein-coupled receptor kinase 4 (EC:2.7.11.16)
Alternative name(s):
G protein-coupled receptor kinase GRK4
Gene namesi
Name:Grk4
Synonyms:Gprk2l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:95801. Grk4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000859681 – 574G protein-coupled receptor kinase 4Add BLAST574

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei484PhosphoserineBy similarity1

Post-translational modificationi

Palmitoylated.By similarity

Keywords - PTMi

Acetylation, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiO70291.
PaxDbiO70291.
PeptideAtlasiO70291.
PRIDEiO70291.

PTM databases

PhosphoSitePlusiO70291.

Expressioni

Gene expression databases

BgeeiENSMUSG00000052783.
CleanExiMM_GRK4.
ExpressionAtlasiO70291. baseline and differential.
GenevisibleiO70291. MM.

Interactioni

Subunit structurei

Interacts with DRD3.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000001112.

Structurei

3D structure databases

ProteinModelPortaliO70291.
SMRiO70291.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini51 – 171RGSPROSITE-ProRule annotationAdd BLAST121
Domaini186 – 448Protein kinasePROSITE-ProRule annotationAdd BLAST263
Domaini449 – 514AGC-kinase C-terminalAdd BLAST66

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 153N-terminalAdd BLAST153

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 RGS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0986. Eukaryota.
ENOG410YRQZ. LUCA.
GeneTreeiENSGT00860000133699.
HOGENOMiHOG000006742.
HOVERGENiHBG004532.
InParanoidiO70291.
KOiK08291.
OMAiHSIEKDY.
OrthoDBiEOG091G062G.
TreeFamiTF313940.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. RGS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR00717. GPCRKINASE.
SMARTiSM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O70291-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELENFVANN LLLKARLGFN KQTGRSKKWR ELLKFPPVSM CTELRWSIEK
60 70 80 90 100
DFSSLCDKQP IGRLLFRQFC DTKPDLKRCI EFLDAVAEYE VTIEEEQREF
110 120 130 140 150
GLAIFSRFFK EKSEVPLPEI PPDIVKECKW NLKQNSPSQN VFEECAGIVC
160 170 180 190 200
KYLSETPFEE YQESTYFNRF LQWKWLERRP VTKNTFRQYR VLGKGGFGEV
210 220 230 240 250
CACQVRATGK MYACKKLEKK RIKKRKGEAM ALNEKRILEK LHSRFVVSLA
260 270 280 290 300
YTYETKDALC LVLTIMNGGD LKYHIYNLGD PGFEEPRAVF YAAELCCGLE
310 320 330 340 350
DLQRKRIVYR DLKPENILLD DHGHIRISDL GLAMEVPEGE MVRGRVGTVG
360 370 380 390 400
YMAPEIINHE KYTFSPDWWG LGCLIYEMIA GHSPFRKYKE KVNREELERR
410 420 430 440 450
VKNETEEYSE RFSEDAKSIC SMLLIKDPSK RLGCQRDGVS AVKQHPIFKD
460 470 480 490 500
INFSRLEANM LDPPFIPDPQ AIYCRNILDI GQFSVVKGVN LDTNDEIFYA
510 520 530 540 550
EFATGSVTIP WQNEMIESGC FKDLNENEDD LSSLEKYKMC SSILRPKRNF
560 570
FRRLFRRTGC LNIALSEERE PTEH
Length:574
Mass (Da):66,928
Last modified:July 27, 2011 - v2
Checksum:i7D7D6F46C9C044B6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6F → M in AAC09266 (PubMed:10506199).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF040745 mRNA. Translation: AAC09266.1.
AK132685 mRNA. Translation: BAE21301.1.
CH466524 Genomic DNA. Translation: EDL37466.1.
BC150691 mRNA. Translation: AAI50692.1.
CCDSiCCDS19219.1.
RefSeqiNP_001074212.1. NM_001080743.1.
NP_062370.2. NM_019497.2.
UniGeneiMm.117076.

Genome annotation databases

EnsembliENSMUST00000001112; ENSMUSP00000001112; ENSMUSG00000052783.
GeneIDi14772.
KEGGimmu:14772.
UCSCiuc008xcz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF040745 mRNA. Translation: AAC09266.1.
AK132685 mRNA. Translation: BAE21301.1.
CH466524 Genomic DNA. Translation: EDL37466.1.
BC150691 mRNA. Translation: AAI50692.1.
CCDSiCCDS19219.1.
RefSeqiNP_001074212.1. NM_001080743.1.
NP_062370.2. NM_019497.2.
UniGeneiMm.117076.

3D structure databases

ProteinModelPortaliO70291.
SMRiO70291.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000001112.

PTM databases

PhosphoSitePlusiO70291.

Proteomic databases

MaxQBiO70291.
PaxDbiO70291.
PeptideAtlasiO70291.
PRIDEiO70291.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000001112; ENSMUSP00000001112; ENSMUSG00000052783.
GeneIDi14772.
KEGGimmu:14772.
UCSCiuc008xcz.1. mouse.

Organism-specific databases

CTDi2868.
MGIiMGI:95801. Grk4.

Phylogenomic databases

eggNOGiKOG0986. Eukaryota.
ENOG410YRQZ. LUCA.
GeneTreeiENSGT00860000133699.
HOGENOMiHOG000006742.
HOVERGENiHBG004532.
InParanoidiO70291.
KOiK08291.
OMAiHSIEKDY.
OrthoDBiEOG091G062G.
TreeFamiTF313940.

Enzyme and pathway databases

ReactomeiR-MMU-2514859. Inactivation, recovery and regulation of the phototransduction cascade.

Miscellaneous databases

PROiO70291.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000052783.
CleanExiMM_GRK4.
ExpressionAtlasiO70291. baseline and differential.
GenevisibleiO70291. MM.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. RGS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR00717. GPCRKINASE.
SMARTiSM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGRK4_MOUSE
AccessioniPrimary (citable) accession number: O70291
Secondary accession number(s): Q3V151
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.