O70291 (GRK4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
G protein-coupled receptor kinase 4
EC=2.7.11.16

Alternative name(s):
G protein-coupled receptor kinase GRK4

Gene names

Name:	Grk4
Synonyms:	Gprk2l

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	574 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Specifically phosphorylates the activated forms of G protein-coupled receptors.
Catalytic activity	ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.
Enzyme regulation	Inhibited by heparin By similarity.
Subunit structure	Interacts with DRD3 By similarity.
Subcellular location	Cytoplasm. Cytoplasm › cell cortex By similarity.
Post-translational modification	Palmitoylated By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain. Contains 1 RGS domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor internalization Inferred from electronic annotation. Source: Compara signal transduction Inferred from electronic annotation. Source: InterPro termination of G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular_component	cell cortex Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Inferred from electronic annotation. Source: Compara dendrite Inferred from electronic annotation. Source: Compara neuronal cell body Inferred from electronic annotation. Source: Compara
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW G-protein coupled receptor kinase activity Inferred from electronic annotation. Source: EC rhodopsin kinase activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 574	574	G protein-coupled receptor kinase 4		PRO_0000085968
Regions
	Domain	51 – 171	121	RGS
	Domain	186 – 448	263	Protein kinase
	Domain	449 – 514	66	AGC-kinase C-terminal
	Nucleotide binding	192 – 200	9	ATP By similarity
	Region	1 – 153	153	N-terminal
Sites
	Active site	311	1	Proton acceptor By similarity
	Binding site	215	1	ATP By similarity
Experimental info
	Sequence conflict	6	1	F → M in AAC09266. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O70291 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 7D7D6F46C9C044B6	FASTA	574	66,928
	10 20 30 40 50 60 MELENFVANN LLLKARLGFN KQTGRSKKWR ELLKFPPVSM CTELRWSIEK DFSSLCDKQP 70 80 90 100 110 120 IGRLLFRQFC DTKPDLKRCI EFLDAVAEYE VTIEEEQREF GLAIFSRFFK EKSEVPLPEI 130 140 150 160 170 180 PPDIVKECKW NLKQNSPSQN VFEECAGIVC KYLSETPFEE YQESTYFNRF LQWKWLERRP 190 200 210 220 230 240 VTKNTFRQYR VLGKGGFGEV CACQVRATGK MYACKKLEKK RIKKRKGEAM ALNEKRILEK 250 260 270 280 290 300 LHSRFVVSLA YTYETKDALC LVLTIMNGGD LKYHIYNLGD PGFEEPRAVF YAAELCCGLE 310 320 330 340 350 360 DLQRKRIVYR DLKPENILLD DHGHIRISDL GLAMEVPEGE MVRGRVGTVG YMAPEIINHE 370 380 390 400 410 420 KYTFSPDWWG LGCLIYEMIA GHSPFRKYKE KVNREELERR VKNETEEYSE RFSEDAKSIC 430 440 450 460 470 480 SMLLIKDPSK RLGCQRDGVS AVKQHPIFKD INFSRLEANM LDPPFIPDPQ AIYCRNILDI 490 500 510 520 530 540 GQFSVVKGVN LDTNDEIFYA EFATGSVTIP WQNEMIESGC FKDLNENEDD LSSLEKYKMC 550 560 570 SSILRPKRNF FRRLFRRTGC LNIALSEERE PTEH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The GRK4 subfamily of G protein-coupled receptor kinases. Alternative splicing, gene organization, and sequence conservation." Premont R.T., Macrae A.D., Aparicio S.A., Kendall H.E., Welch J.E., Lefkowitz R.J. J. Biol. Chem. 274:29381-29389(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Testis.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF040745 mRNA. Translation: AAC09266.1. AK132685 mRNA. Translation: BAE21301.1. CH466524 Genomic DNA. Translation: EDL37466.1. BC150691 mRNA. Translation: AAI50692.1.
IPI	IPI00116586.
RefSeq	NP_001074212.1. NM_001080743.1. NP_062370.2. NM_019497.2.
UniGene	Mm.117076.
3D structure databases
ProteinModelPortal	O70291.
SMR	O70291. Positions 2-525.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000001112.
PTM databases
PhosphoSite	O70291.
Proteomic databases
PRIDE	O70291.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000001112; ENSMUSP00000001112; ENSMUSG00000052783.
GeneID	14772.
KEGG	mmu:14772.
Organism-specific databases
CTD	2868.
MGI	MGI:95801. Grk4.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00550000074318.
HOGENOM	HOG000006742.
HOVERGEN	HBG004532.
InParanoid	Q3V151.
KO	K08291.
OMA	HSIEKDY.
OrthoDB	EOG4HHP24.
Gene expression databases
ArrayExpress	O70291.
Bgee	O70291.
CleanEx	MM_GRK4.
Genevestigator	O70291.
GermOnline	ENSMUSG00000052783. Mus musculus.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR000239. GPCR_kinase. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR000342. Regulat_G_prot_signal. IPR016137. Regulat_G_prot_signal_superfam. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. PF00615. RGS. 1 hit. [Graphical view]
PRINTS	PR00717. GPCRKINASE.
SMART	SM00315. RGS. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit. SSF48097. Regulat_G_prot_signal_superfam. 1 hit.
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS50132. RGS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	286867.
SOURCE	Search...

Entry information

Entry name

GRK4_MOUSE

Accession

Primary (citable) accession number: O70291
Secondary accession number(s): Q3V151

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	July 27, 2011
Last modified:	April 3, 2013
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program