O70283 (WNT2B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Wnt-2b

Alternative name(s):
Protein Wnt-13

Gene names

Name:	Wnt2b
Synonyms:	Wnt13

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	389 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters By similarity.
Subcellular location	Secreted › extracellular space › extracellular matrix.
Post-translational modification	Palmitoylation at Ser-241 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-105. Palmitoylation is necessary for proper trafficking to cell surface By similarity.
Sequence similarities	Belongs to the Wnt family.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Extracellular matrix Secreted
Domain	Signal
Molecular function	Developmental protein
PTM	Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway, calcium modulating pathway Inferred from Biological aspect of Ancestor. Source: RefGenome canonical Wnt receptor signaling pathway Inferred from genetic interaction PubMed 19686689. Source: MGI chondrocyte differentiation Inferred from electronic annotation. Source: Compara dorsal/ventral axis specification Inferred from Biological aspect of Ancestor. Source: RefGenome forebrain regionalization Inferred from expression pattern PubMed 15164427. Source: UniProtKB hematopoietic stem cell proliferation Inferred from electronic annotation. Source: Compara lung induction Inferred from genetic interaction PubMed 19686689. Source: MGI male gonad development Inferred from electronic annotation. Source: Compara mesenchymal-epithelial cell signaling Inferred from direct assay PubMed 11507767. Source: MGI negative regulation of transcription from RNA polymerase II promoter Inferred from Biological aspect of Ancestor. Source: RefGenome organ morphogenesis Traceable author statement PubMed 9889131. Source: MGI positive regulation of branching involved in ureteric bud morphogenesis Inferred from direct assay PubMed 11507767. Source: MGI positive regulation of canonical Wnt receptor signaling pathway Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular space Inferred from Biological aspect of Ancestor. Source: RefGenome plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	frizzled-2 binding Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – ?		Potential
	Chain	? – 389		Protein Wnt-2b		PRO_0000041414
Amino acid modifications
	Lipidation	105	1	S-palmitoyl cysteine By similarity
	Lipidation	241	1	O-palmitoyl serine; by PORCN By similarity
	Glycosylation	115	1	N-linked (GlcNAc...) Potential
	Glycosylation	281	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	313	1	A → S in AAC25397. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O70283 [UniParc]. Last modified July 27, 2011. Version 3. Checksum: 64F956BB9A00CEEB	FASTA	389	43,753
	10 20 30 40 50 60 MLKLQGEDEA AQLAPRRARV PVPRPTAPDV SPSSARLGLA CLLLLLLLTL PARVDTSWWY 70 80 90 100 110 120 IGALGARVIC DNIPGLVSRQ RQLCQRYPDI MRSVGEGARE WIRECQHQFR HHRWNCTTLD 130 140 150 160 170 180 RDHTVFGRAM LRSSREAAFV YAISSAGVVH AITRACSQGE LSVCSCDPYT RGRHHDQRGD 190 200 210 220 230 240 FDWGGCSDNI HYGVRFAKAF VDAKEKRLKD ARALMNLHNN RCGRTAVRRF LKLECKCHGV 250 260 270 280 290 300 SGSCTLRTCW RALSDFRRTG DYLRRRYDGA VQVTATQDGA NFTAARQGYR HATRTDLVYF 310 320 330 340 350 360 DNSPDYCVLD KAAGSLGTAG RVCSKTSKGT DGCEIMCCGR GYDTTRVTRV TQCECKFHWC 370 380 CAVRCKECRN TVDVHTCKAP KKAEWLDQT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and expression of Wnt13, a novel mouse Wnt2 related gene." Zakin L.D.J., Mazan S., Maury M., Martin N., Guenet J.-L., Brulet P. Mech. Dev. 73:107-116(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Urinary bladder.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"The hem of the embryonic cerebral cortex is defined by the expression of multiple Wnt genes and is compromised in Gli3-deficient mice." Grove E.A., Tole S., Limon J., Yip L., Ragsdale C.W. Development 125:2315-2325(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 154-389. Strain: NIH Swiss.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF070988 mRNA. Translation: AAC25397.1. AK035653 mRNA. Translation: BAC29139.1. CH466608 Genomic DNA. Translation: EDL07557.1. BC119276 mRNA. Translation: AAI19277.1. BC119278 mRNA. Translation: AAI19279.1. AF038384 mRNA. Translation: AAC40123.1.
IPI	IPI00116556.
RefSeq	NP_033546.2. NM_009520.3.
UniGene	Mm.10740.
3D structure databases
ProteinModelPortal	O70283.
ModBase	Search...
PTM databases
PhosphoSite	O70283.
Proteomic databases
PRIDE	O70283.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000029429; ENSMUSP00000029429; ENSMUSG00000027840.
GeneID	22414.
KEGG	mmu:22414.
Organism-specific databases
CTD	7482.
MGI	MGI:1261834. Wnt2b.
Phylogenomic databases
eggNOG	NOG284879.
GeneTree	ENSGT00700000104304.
HOGENOM	HOG000039529.
HOVERGEN	HBG001595.
InParanoid	Q544L3.
KO	K00182.
OMA	PDIMQAI.
OrthoDB	EOG4255SV.
Gene expression databases
Bgee	O70283.
CleanEx	MM_WNT2B.
Genevestigator	O70283.
GermOnline	ENSMUSG00000027840. Mus musculus.
Family and domain databases
InterPro	IPR005817. Wnt. IPR009140. Wnt2. IPR018161. Wnt_CS. [Graphical view]
PANTHER	PTHR12027. PTHR12027. 1 hit. PTHR12027:SF21. PTHR12027:SF21. 1 hit.
Pfam	PF00110. wnt. 1 hit. [Graphical view]
PRINTS	PR01842. WNT2PROTEIN. PR01349. WNTPROTEIN.
SMART	SM00097. WNT1. 1 hit. [Graphical view]
PROSITE	PS00246. WNT1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	302825.
SOURCE	Search...

Entry information

Entry name

WNT2B_MOUSE

Accession

Primary (citable) accession number: O70283
Secondary accession number(s): O88530, Q544L3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 27, 2011
Last modified:	April 3, 2013
This is version 97 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program