Reviewed, UniProtKB/Swiss-Prot O70281 (TPST1_MOUSE)

Names and origin

Protein names

Recommended name:
    Protein-tyrosine sulfotransferase 1
    EC=2.8.2.20
Alternative name(s):
    Tyrosylprotein sulfotransferase-1
      Short name=TPST-1

Gene names

Name:

Tpst1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides.
Catalytic activity	3'-phosphoadenylyl sulfate + protein tyrosine = adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the protein sulfotransferase family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-tyrosine sulfotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	Protein-tyrosine sulfotransferase 1		PRO_0000189827
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 25	17	Signal-anchor for type II membrane protein Potential
	Topological domain	26 – 370	345	Lumenal Potential
Amino acid modifications
	Glycosylation	60	1	N-linked (GlcNAc...) Potential
	Glycosylation	262	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O70281-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 4B85624D23C7800D	FASTA	370	42,132
	10 20 30 40 50 60 MVGKLKQNLL LACLVISSVT VFYLGQHAME CHHRIEERSQ PARLENPKAT VRAGLDIKAN 70 80 90 100 110 120 KTFTYHKDMP LIFIGGVPRS GTTLMRAMLD AHPDIRCGEE TRVIPRILAL KQMWSRSSKE 130 140 150 160 170 180 KIRLDEAGVT DEVLDSAMQA FLLEVIVKHG EPAPYLCNKD PFALKSLTYL ARLFPNAKFL 190 200 210 220 230 240 LMVRDGRASV HSMISRKVTI AGFDLNSYRD CLTKWNRAIE TMYNQCMEVG YKKCMLVHYE 250 260 270 280 290 300 QLVLHPERWM RTLLKFLHIP WNHSVLHHEE MIGKAGGVSL SKVERSTDQV IKPVNVGALS 310 320 330 340 350 360 KWVGKIPPDV LQDMAVIAPM LAKLGYDPYA NPPNYGKPDP KILENTRRVY KGEFQLPDFL 370 KEKPQTEQVE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tyrosylprotein sulfotransferase: purification and molecular cloning of an enzyme that catalyzes tyrosine O-sulfation, a common posttranslational modification of eukaryotic proteins." Ouyang Y.-B., Lane W.S., Moore K.L. Proc. Natl. Acad. Sci. U.S.A. 95:2896-2901(1998) [PubMed: 9501187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Mammary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF038008 mRNA. Translation: AAC13551.1. BC012666 mRNA. Translation: AAH12666.1.
IPI	IPI00116552.
RefSeq	NP_001123948.1. NP_038865.1.
UniGene	Mm.332720
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O70281.
Proteomic databases
PRIDE	O70281.
Genome annotation databases
Ensembl	ENSMUST00000040721; ENSMUSP00000035614; ENSMUSG00000034118; Mus musculus. [Genome view] ENSMUST00000118993; ENSMUSP00000112571; ENSMUSG00000034118; Mus musculus. [Genome view]
GeneID	22021.
KEGG	mmu:22021.
UCSC	uc008zuc.1. mouse.
Organism-specific databases
CTD	22021.
MGI	MGI:1298231. Tpst1.
Phylogenomic databases
HOVERGEN	O70281.
OMA	TRRVFKG.
Enzyme and pathway databases
BRENDA	2.8.2.20. 244.
Gene expression databases
ArrayExpress	O70281.
Bgee	O70281.
CleanEx	MM_TPST1.
Genevestigator	O70281.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	301752.
SOURCE	Search...

Entry information

Entry name

TPST1_MOUSE

Accession

Primary (citable) accession number: O70281

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)