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Protein

Tripartite motif-containing protein 3

Gene

Trim3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri22 – 6342RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri110 – 15142B box-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Tripartite motif-containing protein 3
Alternative name(s):
Brain-expressed RING finger protein
RING finger protein 22
Gene namesi
Name:Trim3
Synonyms:Berp, Rnf22
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi70074. Trim3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • early endosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 744743Tripartite motif-containing protein 3PRO_0000056199Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei7 – 71PhosphoserineCombined sources
Modified residuei427 – 4271PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiO70277.
PRIDEiO70277.

PTM databases

PhosphoSiteiO70277.
SwissPalmiO70277.

Expressioni

Tissue specificityi

Highly expressed in the brain, moderate levels in the lung, very low levels in the liver, kidney and heart. In the brain, expression was highest in the cerebellum.

Interactioni

Subunit structurei

Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3. Interacts with ZFYVE28/LST2 (By similarity). Associates with myosin-Vb (MYO5B) and alpha-actinin-4 (ACTN4).By similarity2 Publications

Protein-protein interaction databases

IntActiO70277. 1 interaction.
STRINGi10116.ENSRNOP00000024850.

Structurei

3D structure databases

ProteinModelPortaliO70277.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati317 – 418102FilaminAdd
BLAST
Repeati473 – 51644NHL 1Add
BLAST
Repeati520 – 56344NHL 2Add
BLAST
Repeati564 – 60542NHL 3Add
BLAST
Repeati609 – 65244NHL 4Add
BLAST
Repeati656 – 69944NHL 5Add
BLAST
Repeati700 – 74344NHL 6Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili153 – 22472Sequence analysisAdd
BLAST

Domaini

The interaction with MYO5B is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades.

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated
Contains 1 B box-type zinc finger.PROSITE-ProRule annotation
Contains 1 filamin repeat.PROSITE-ProRule annotation
Contains 6 NHL repeats.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri22 – 6342RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri110 – 15142B box-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410ITF9. Eukaryota.
ENOG410ZAM4. LUCA.
HOGENOMiHOG000220817.
HOVERGENiHBG054843.
InParanoidiO70277.
KOiK11997.
PhylomeDBiO70277.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
2.60.40.10. 1 hit.
3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR003649. Bbox_C.
IPR017868. Filamin/ABP280_repeat-like.
IPR001298. Filamin/ABP280_rpt.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR001258. NHL_repeat.
IPR013017. NHL_repeat_subgr.
IPR027370. Znf-RING_LisH.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00630. Filamin. 1 hit.
PF01436. NHL. 6 hits.
PF00643. zf-B_box. 1 hit.
PF13445. zf-RING_UBOX. 1 hit.
[Graphical view]
SMARTiSM00502. BBC. 1 hit.
SM00336. BBOX. 1 hit.
SM00557. IG_FLMN. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.
PROSITEiPS50194. FILAMIN_REPEAT. 1 hit.
PS51125. NHL. 6 hits.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O70277-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKREDSPGP EVQPMDKQFL VCSICLDRYR CPKVLPCLHT FCERCLQNYI
60 70 80 90 100
PPQSLTLSCP VCRQTSILPE QGVSALQNNF FISSLMEAMQ QAPDGAHDPE
110 120 130 140 150
DPHPLSAVAG RPLSCPNHEG KTMEFYCEAC ETAMCGECRA GEHREHGTVL
160 170 180 190 200
LRDVVEQHKA ALQRQLEAVR GRLPQLSAAI ALVGGISQQL QERKAEALAQ
210 220 230 240 250
ISAAFEDLEQ ALQQRKQALV SDLESICGAK QKVLQTQLDT LRQGQEHIGS
260 270 280 290 300
SCSFAEQALR LGSAPEVLLV RKHMRERLAA LAAQAFPERP HENAQLELVL
310 320 330 340 350
EVDGLRRSVL NLGALLTTSA AAHETVATGE GLRQALVGQP ASLTVTTKDK
360 370 380 390 400
DGRLVRTGSA ELCAEITGPD GMRLAVPVVD HKNGTYELVY TARTEGDLLL
410 420 430 440 450
SVLLYGQPVR GSPFRVRALR PGDLPPSPDD VKRRVKSPGG PGSHVRQKAV
460 470 480 490 500
RRPSSMYSTG GKRKDNPIVD ELVFRVGSRG REKGEFTNLH PLSAASSGRI
510 520 530 540 550
VVADSNNQCI QVFSNEGQFK FRFGVRGRSP GQLQRPTGVA VDTNGDIIVA
560 570 580 590 600
DYDNRWVSIF SPEGKFKTKI GAGRLMGPKG VAVDRNGHII VVDNKSCCVF
610 620 630 640 650
TFQPNGKLVG RFGGRGATDR HFAGPHFVAV NNKNEIVVTD FHNHSVKVYS
660 670 680 690 700
ADGEFLFKFG SHGEGNGQFN APTGVAVDSN GNIIVADWGN SRIQVFDSSG
710 720 730 740
SFLSYINTSA EPLYGPQGLA LTSDGHVVVA DAGNHCFKAY RYLQ
Length:744
Mass (Da):80,796
Last modified:August 1, 1998 - v1
Checksum:i4BFF839B7A4809EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036255 mRNA. Translation: AAC17997.1.
RefSeqiNP_113974.1. NM_031786.1.
UniGeneiRn.203982.

Genome annotation databases

GeneIDi83616.
KEGGirno:83616.
UCSCiRGD:70074. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036255 mRNA. Translation: AAC17997.1.
RefSeqiNP_113974.1. NM_031786.1.
UniGeneiRn.203982.

3D structure databases

ProteinModelPortaliO70277.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO70277. 1 interaction.
STRINGi10116.ENSRNOP00000024850.

PTM databases

PhosphoSiteiO70277.
SwissPalmiO70277.

Proteomic databases

PaxDbiO70277.
PRIDEiO70277.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi83616.
KEGGirno:83616.
UCSCiRGD:70074. rat.

Organism-specific databases

CTDi10612.
RGDi70074. Trim3.

Phylogenomic databases

eggNOGiENOG410ITF9. Eukaryota.
ENOG410ZAM4. LUCA.
HOGENOMiHOG000220817.
HOVERGENiHBG054843.
InParanoidiO70277.
KOiK11997.
PhylomeDBiO70277.

Miscellaneous databases

NextBioi616169.
PROiO70277.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
2.60.40.10. 1 hit.
3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR003649. Bbox_C.
IPR017868. Filamin/ABP280_repeat-like.
IPR001298. Filamin/ABP280_rpt.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR001258. NHL_repeat.
IPR013017. NHL_repeat_subgr.
IPR027370. Znf-RING_LisH.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00630. Filamin. 1 hit.
PF01436. NHL. 6 hits.
PF00643. zf-B_box. 1 hit.
PF13445. zf-RING_UBOX. 1 hit.
[Graphical view]
SMARTiSM00502. BBC. 1 hit.
SM00336. BBOX. 1 hit.
SM00557. IG_FLMN. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.
PROSITEiPS50194. FILAMIN_REPEAT. 1 hit.
PS51125. NHL. 6 hits.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a novel RING finger protein that interacts with class V myosins."
    El-Husseini A.E.-D., Vincent S.R.
    J. Biol. Chem. 274:19771-19777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH MYO5B.
    Tissue: Brain.
  2. Cited for: INTERACTION WITH ACTN4.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7 AND SER-427, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTRIM3_RAT
AccessioniPrimary (citable) accession number: O70277
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: August 1, 1998
Last modified: May 11, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.