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O70273

- EHF_MOUSE

UniProt

O70273 - EHF_MOUSE

Protein

ETS homologous factor

Gene

Ehf

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Transcriptional activator that may play a role in regulating epithelial cell differentiation and proliferation. May act as a repressor for a specific subset of ETS/AP-1-responsive genes, and as a modulator of the nuclear response to mitogen-activated protein kinase signaling cascades. Binds to DNA sequences containing the consensus nucleotide core sequence GGAA. Involved in regulation of TNFRSF10B/DR5 expression through Ets-binding sequences on the TNFRSF10B/DR5 promoter By similarity.By similarity1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi207 – 28983ETSPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB
    2. sequence-specific DNA binding Source: InterPro
    3. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: RefGenome
    4. sequence-specific DNA binding transcription factor activity Source: UniProtKB

    GO - Biological processi

    1. cell differentiation Source: RefGenome
    2. epithelial cell differentiation Source: UniProtKB
    3. positive regulation of transcription, DNA-templated Source: UniProtKB
    4. regulation of transcription from RNA polymerase II promoter Source: RefGenome
    5. transcription from RNA polymerase II promoter Source: GOC

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ETS homologous factor
    Alternative name(s):
    ETS domain-containing transcription factor
    Gene namesi
    Name:EhfImported
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:1270840. Ehf.

    Subcellular locationi

    Nucleus By similarityPROSITE-ProRule annotation

    GO - Cellular componenti

    1. nucleus Source: RefGenome

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 300300ETS homologous factorPRO_0000257970Add
    BLAST

    Proteomic databases

    MaxQBiO70273.
    PRIDEiO70273.

    PTM databases

    PhosphoSiteiO70273.

    Expressioni

    Tissue specificityi

    Highly expressed in kidney and lung, weakly in skeletal muscle, heart, and liver, and not detected in brain, spleen or testis.1 Publication

    Gene expression databases

    ArrayExpressiO70273.
    BgeeiO70273.
    CleanExiMM_EHF.
    GenevestigatoriO70273.

    Interactioni

    Protein-protein interaction databases

    BioGridi199409. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliO70273.
    SMRiO70273. Positions 40-111, 206-299.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini29 – 11587PNTPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The PNT domain acts as a transcriptional activator.1 Publication

    Sequence similaritiesi

    Belongs to the ETS family.Sequence Analysis
    Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 PNT (pointed) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG307806.
    GeneTreeiENSGT00750000117299.
    HOGENOMiHOG000232188.
    HOVERGENiHBG074143.
    InParanoidiO70273.
    KOiK17102.
    OMAiVMNLNPG.
    OrthoDBiEOG7M0NRR.
    PhylomeDBiO70273.
    TreeFamiTF318679.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    1.10.150.50. 1 hit.
    InterProiIPR000418. Ets_dom.
    IPR003118. Pointed_dom.
    IPR013761. SAM/pointed.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00178. Ets. 1 hit.
    PF02198. SAM_PNT. 1 hit.
    [Graphical view]
    PRINTSiPR00454. ETSDOMAIN.
    SMARTiSM00413. ETS. 1 hit.
    SM00251. SAM_PNT. 1 hit.
    [Graphical view]
    SUPFAMiSSF47769. SSF47769. 1 hit.
    PROSITEiPS50061. ETS_DOMAIN_3. 1 hit.
    PS51433. PNT. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 11 Publication (identifier: O70273-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MILEGSGVMN LNPANNLLHQ QPAWTDSYPT CNVSSGFFGS QWHEIHPQYW    50
    TKYQVWEWLQ HLLDTNQLDA SCIPFQEFDI SGEHLCSMSL QEFTRAAGSA 100
    GQLLYSNLQH LKWNGQCSSD LFQSAHNVIV KTEQTDPSIM NTWKEENYLY 150
    DPSYGSTVDL LDSKTFCRAQ ISMTTSSHLP VAESPDMKKE QDHPVKSHTK 200
    KHNPRGTHLW EFIRDILLSP DKNPGLIKWE DRSEGIFRFL KSEAVAQLWG 250
    KKKNNSSMTY EKLSRAMRYY YKREILERVD GRRLVYKFGK NARGWRENEN 300
    Length:300
    Mass (Da):34,903
    Last modified:August 1, 1998 - v1
    Checksum:i91D9477EFB874747
    GO
    Isoform 21 Publication (identifier: O70273-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         79-93: Missing.

    Note: No experimental confirmation available.Curated

    Show »
    Length:285
    Mass (Da):33,225
    Checksum:i91398C527C65955F
    GO
    Isoform 31 Publication (identifier: O70273-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         200-211: KKHNPRGTHLWE → YFIIELLLSESR
         212-300: Missing.

    Note: No experimental confirmation available.Curated

    Show »
    Length:211
    Mass (Da):24,139
    Checksum:i54F8846761D533CD
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei79 – 9315Missing in isoform 2. 1 PublicationVSP_052191Add
    BLAST
    Alternative sequencei200 – 21112KKHNP…THLWE → YFIIELLLSESR in isoform 3. 1 PublicationVSP_052192Add
    BLAST
    Alternative sequencei212 – 30089Missing in isoform 3. 1 PublicationVSP_052193Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF035527 mRNA. Translation: AAC40119.1.
    BC005520 mRNA. Translation: AAH05520.1.
    BC006789 mRNA. Translation: AAH06789.1.
    BC008249 mRNA. Translation: AAH08249.1.
    CCDSiCCDS16475.1. [O70273-1]
    PIRiJW0048.
    RefSeqiNP_031940.1. NM_007914.3. [O70273-1]
    XP_006498760.1. XM_006498697.1. [O70273-1]
    XP_006498761.1. XM_006498698.1. [O70273-1]
    XP_006498762.1. XM_006498699.1. [O70273-1]
    XP_006498763.1. XM_006498700.1. [O70273-1]
    XP_006498764.1. XM_006498701.1. [O70273-1]
    XP_006498765.1. XM_006498702.1. [O70273-1]
    XP_006500821.1. XM_006500758.1. [O70273-1]
    XP_006500822.1. XM_006500759.1. [O70273-1]
    XP_006500823.1. XM_006500760.1. [O70273-1]
    XP_006500824.1. XM_006500761.1. [O70273-1]
    XP_006500825.1. XM_006500762.1. [O70273-1]
    XP_006500826.1. XM_006500763.1. [O70273-1]
    UniGeneiMm.10724.

    Genome annotation databases

    EnsembliENSMUST00000090475; ENSMUSP00000087961; ENSMUSG00000012350. [O70273-1]
    ENSMUST00000111174; ENSMUSP00000106805; ENSMUSG00000012350. [O70273-1]
    GeneIDi13661.
    KEGGimmu:13661.
    UCSCiuc008lio.1. mouse. [O70273-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF035527 mRNA. Translation: AAC40119.1 .
    BC005520 mRNA. Translation: AAH05520.1 .
    BC006789 mRNA. Translation: AAH06789.1 .
    BC008249 mRNA. Translation: AAH08249.1 .
    CCDSi CCDS16475.1. [O70273-1 ]
    PIRi JW0048.
    RefSeqi NP_031940.1. NM_007914.3. [O70273-1 ]
    XP_006498760.1. XM_006498697.1. [O70273-1 ]
    XP_006498761.1. XM_006498698.1. [O70273-1 ]
    XP_006498762.1. XM_006498699.1. [O70273-1 ]
    XP_006498763.1. XM_006498700.1. [O70273-1 ]
    XP_006498764.1. XM_006498701.1. [O70273-1 ]
    XP_006498765.1. XM_006498702.1. [O70273-1 ]
    XP_006500821.1. XM_006500758.1. [O70273-1 ]
    XP_006500822.1. XM_006500759.1. [O70273-1 ]
    XP_006500823.1. XM_006500760.1. [O70273-1 ]
    XP_006500824.1. XM_006500761.1. [O70273-1 ]
    XP_006500825.1. XM_006500762.1. [O70273-1 ]
    XP_006500826.1. XM_006500763.1. [O70273-1 ]
    UniGenei Mm.10724.

    3D structure databases

    ProteinModelPortali O70273.
    SMRi O70273. Positions 40-111, 206-299.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199409. 1 interaction.

    PTM databases

    PhosphoSitei O70273.

    Proteomic databases

    MaxQBi O70273.
    PRIDEi O70273.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000090475 ; ENSMUSP00000087961 ; ENSMUSG00000012350 . [O70273-1 ]
    ENSMUST00000111174 ; ENSMUSP00000106805 ; ENSMUSG00000012350 . [O70273-1 ]
    GeneIDi 13661.
    KEGGi mmu:13661.
    UCSCi uc008lio.1. mouse. [O70273-1 ]

    Organism-specific databases

    CTDi 26298.
    MGIi MGI:1270840. Ehf.

    Phylogenomic databases

    eggNOGi NOG307806.
    GeneTreei ENSGT00750000117299.
    HOGENOMi HOG000232188.
    HOVERGENi HBG074143.
    InParanoidi O70273.
    KOi K17102.
    OMAi VMNLNPG.
    OrthoDBi EOG7M0NRR.
    PhylomeDBi O70273.
    TreeFami TF318679.

    Miscellaneous databases

    NextBioi 284404.
    PROi O70273.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O70273.
    Bgeei O70273.
    CleanExi MM_EHF.
    Genevestigatori O70273.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    1.10.150.50. 1 hit.
    InterProi IPR000418. Ets_dom.
    IPR003118. Pointed_dom.
    IPR013761. SAM/pointed.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00178. Ets. 1 hit.
    PF02198. SAM_PNT. 1 hit.
    [Graphical view ]
    PRINTSi PR00454. ETSDOMAIN.
    SMARTi SM00413. ETS. 1 hit.
    SM00251. SAM_PNT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47769. SSF47769. 1 hit.
    PROSITEi PS50061. ETS_DOMAIN_3. 1 hit.
    PS51433. PNT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and expression of Ehf, a new member of the ets transcription factor/oncoprotein gene family."
      Bochert M.A., Kleinbaum L.A., Sun L.-Y., Burton F.H.
      Biochem. Biophys. Res. Commun. 246:176-181(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
      Tissue: PituitaryImported.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 222-300 (ISOFORMS 1/2).
      Strain: Czech IIImported and FVB/NImported.
      Tissue: Mammary glandImported.
    3. "Determination of the consensus DNA-binding sequence and a transcriptional activation domain for ESE-2."
      Choi Y.S., Sinha S.
      Biochem. J. 398:497-507(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: DOMAIN.

    Entry informationi

    Entry nameiEHF_MOUSE
    AccessioniPrimary (citable) accession number: O70273
    Secondary accession number(s): Q922E8, Q922T6, Q99K12
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2006
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3