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O70273 (EHF_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ETS homologous factor
Alternative name(s):
ETS domain-containing transcription factor
Gene names
Name:Ehf
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length300 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcriptional activator that may play a role in regulating epithelial cell differentiation and proliferation. May act as a repressor for a specific subset of ETS/AP-1-responsive genes, and as a modulator of the nuclear response to mitogen-activated protein kinase signaling cascades. Binds to DNA sequences containing the consensus nucleotide core sequence GGAA. Involved in regulation of TNFRSF10B/DR5 expression through Ets-binding sequences on the TNFRSF10B/DR5 promoter By similarity. Ref.3 UniProtKB Q9NZC4

Subcellular location

Nucleus By similarity UniProtKB Q9NZC4.

Tissue specificity

Highly expressed in kidney and lung, weakly in skeletal muscle, heart, and liver, and not detected in brain, spleen or testis. Ref.1

Domain

The PNT domain acts as a transcriptional activator. Ref.3

Sequence similarities

Belongs to the ETS family.

Contains 1 ETS DNA-binding domain.

Contains 1 PNT (pointed) domain.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.1 (identifier: O70273-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 Ref.2 (identifier: O70273-2)

The sequence of this isoform differs from the canonical sequence as follows:
     79-93: Missing.
Note: No experimental confirmation available.
Isoform 3 Ref.2 (identifier: O70273-3)

The sequence of this isoform differs from the canonical sequence as follows:
     200-211: KKHNPRGTHLWE → YFIIELLLSESR
     212-300: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 300300ETS homologous factor
PRO_0000257970

Regions

Domain29 – 11587PNT
DNA binding207 – 28983ETS

Natural variations

Alternative sequence79 – 9315Missing in isoform 2. Ref.2
VSP_052191
Alternative sequence200 – 21112KKHNP…THLWE → YFIIELLLSESR in isoform 3. Ref.2
VSP_052192
Alternative sequence212 – 30089Missing in isoform 3. Ref.2
VSP_052193

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 91D9477EFB874747

FASTA30034,903
        10         20         30         40         50         60 
MILEGSGVMN LNPANNLLHQ QPAWTDSYPT CNVSSGFFGS QWHEIHPQYW TKYQVWEWLQ 

        70         80         90        100        110        120 
HLLDTNQLDA SCIPFQEFDI SGEHLCSMSL QEFTRAAGSA GQLLYSNLQH LKWNGQCSSD 

       130        140        150        160        170        180 
LFQSAHNVIV KTEQTDPSIM NTWKEENYLY DPSYGSTVDL LDSKTFCRAQ ISMTTSSHLP 

       190        200        210        220        230        240 
VAESPDMKKE QDHPVKSHTK KHNPRGTHLW EFIRDILLSP DKNPGLIKWE DRSEGIFRFL 

       250        260        270        280        290        300 
KSEAVAQLWG KKKNNSSMTY EKLSRAMRYY YKREILERVD GRRLVYKFGK NARGWRENEN 

« Hide

Isoform 2 [UniParc].

Checksum: 91398C527C65955F
Show »

FASTA28533,225
Isoform 3 [UniParc].

Checksum: 54F8846761D533CD
Show »

FASTA21124,139

References

« Hide 'large scale' references
[1]"Molecular cloning and expression of Ehf, a new member of the ets transcription factor/oncoprotein gene family."
Bochert M.A., Kleinbaum L.A., Sun L.-Y., Burton F.H.
Biochem. Biophys. Res. Commun. 246:176-181(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
Tissue: Pituitary.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 222-300 (ISOFORMS 1/2).
Strain: Czech II and FVB/N.
Tissue: Mammary gland.
[3]"Determination of the consensus DNA-binding sequence and a transcriptional activation domain for ESE-2."
Choi Y.S., Sinha S.
Biochem. J. 398:497-507(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: DOMAIN.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF035527 mRNA. Translation: AAC40119.1.
BC005520 mRNA. Translation: AAH05520.1.
BC006789 mRNA. Translation: AAH06789.1.
BC008249 mRNA. Translation: AAH08249.1.
CCDSCCDS16475.1. [O70273-1]
PIRJW0048.
RefSeqNP_031940.1. NM_007914.3. [O70273-1]
XP_006498760.1. XM_006498697.1. [O70273-1]
XP_006498761.1. XM_006498698.1. [O70273-1]
XP_006498762.1. XM_006498699.1. [O70273-1]
XP_006498763.1. XM_006498700.1. [O70273-1]
XP_006498764.1. XM_006498701.1. [O70273-1]
XP_006498765.1. XM_006498702.1. [O70273-1]
XP_006500821.1. XM_006500758.1. [O70273-1]
XP_006500822.1. XM_006500759.1. [O70273-1]
XP_006500823.1. XM_006500760.1. [O70273-1]
XP_006500824.1. XM_006500761.1. [O70273-1]
XP_006500825.1. XM_006500762.1. [O70273-1]
XP_006500826.1. XM_006500763.1. [O70273-1]
UniGeneMm.10724.

3D structure databases

ProteinModelPortalO70273.
SMRO70273. Positions 40-111, 206-299.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199409. 1 interaction.

PTM databases

PhosphoSiteO70273.

Proteomic databases

MaxQBO70273.
PRIDEO70273.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000090475; ENSMUSP00000087961; ENSMUSG00000012350. [O70273-1]
ENSMUST00000111174; ENSMUSP00000106805; ENSMUSG00000012350. [O70273-1]
GeneID13661.
KEGGmmu:13661.
UCSCuc008lio.1. mouse. [O70273-1]

Organism-specific databases

CTD26298.
MGIMGI:1270840. Ehf.

Phylogenomic databases

eggNOGNOG307806.
GeneTreeENSGT00750000117299.
HOGENOMHOG000232188.
HOVERGENHBG074143.
InParanoidO70273.
KOK17102.
OMAVMNLNPG.
OrthoDBEOG7M0NRR.
PhylomeDBO70273.
TreeFamTF318679.

Gene expression databases

ArrayExpressO70273.
BgeeO70273.
CleanExMM_EHF.
GenevestigatorO70273.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
1.10.150.50. 1 hit.
InterProIPR000418. Ets_dom.
IPR003118. Pointed_dom.
IPR013761. SAM/pointed.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00178. Ets. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]
PRINTSPR00454. ETSDOMAIN.
SMARTSM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]
SUPFAMSSF47769. SSF47769. 1 hit.
PROSITEPS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio284404.
PROO70273.
SOURCESearch...

Entry information

Entry nameEHF_MOUSE
AccessionPrimary (citable) accession number: O70273
Secondary accession number(s): Q922E8, Q922T6, Q99K12
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: August 1, 1998
Last modified: July 9, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot