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Protein

ETS homologous factor

Gene

Ehf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Transcriptional activator that may play a role in regulating epithelial cell differentiation and proliferation. May act as a repressor for a specific subset of ETS/AP-1-responsive genes, and as a modulator of the nuclear response to mitogen-activated protein kinase signaling cascades. Binds to DNA sequences containing the consensus nucleotide core sequence GGAA. Involved in regulation of TNFRSF10B/DR5 expression through Ets-binding sequences on the TNFRSF10B/DR5 promoter (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi207 – 28983ETSPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: MGI
  3. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: MGI
  4. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: GO_Central
  5. sequence-specific DNA binding transcription factor activity Source: UniProtKB

GO - Biological processi

  1. cell differentiation Source: GO_Central
  2. epithelial cell differentiation Source: UniProtKB
  3. positive regulation of transcription, DNA-templated Source: UniProtKB
  4. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  5. regulation of transcription from RNA polymerase II promoter Source: GO_Central
  6. transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ETS homologous factor
Alternative name(s):
ETS domain-containing transcription factor
Gene namesi
Name:EhfImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:1270840. Ehf.

Subcellular locationi

Nucleus PROSITE-ProRule annotationBy similarity

GO - Cellular componenti

  1. Golgi apparatus Source: MGI
  2. nucleoplasm Source: MGI
  3. nucleus Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 300300ETS homologous factorPRO_0000257970Add
BLAST

Proteomic databases

MaxQBiO70273.
PRIDEiO70273.

PTM databases

PhosphoSiteiO70273.

Expressioni

Tissue specificityi

Highly expressed in kidney and lung, weakly in skeletal muscle, heart, and liver, and not detected in brain, spleen or testis.1 Publication

Gene expression databases

BgeeiO70273.
CleanExiMM_EHF.
ExpressionAtlasiO70273. baseline and differential.
GenevestigatoriO70273.

Interactioni

Protein-protein interaction databases

BioGridi199409. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliO70273.
SMRiO70273. Positions 40-111, 206-299.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 11587PNTPROSITE-ProRule annotationAdd
BLAST

Domaini

The PNT domain acts as a transcriptional activator.1 Publication

Sequence similaritiesi

Belongs to the ETS family.Sequence Analysis
Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation
Contains 1 PNT (pointed) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG307806.
GeneTreeiENSGT00760000118996.
HOGENOMiHOG000232188.
HOVERGENiHBG074143.
InParanoidiO70273.
KOiK17102.
OMAiDENYLYD.
OrthoDBiEOG7M0NRR.
PhylomeDBiO70273.
TreeFamiTF318679.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.50. 1 hit.
InterProiIPR000418. Ets_dom.
IPR003118. Pointed_dom.
IPR013761. SAM/pointed.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: O70273-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MILEGSGVMN LNPANNLLHQ QPAWTDSYPT CNVSSGFFGS QWHEIHPQYW
60 70 80 90 100
TKYQVWEWLQ HLLDTNQLDA SCIPFQEFDI SGEHLCSMSL QEFTRAAGSA
110 120 130 140 150
GQLLYSNLQH LKWNGQCSSD LFQSAHNVIV KTEQTDPSIM NTWKEENYLY
160 170 180 190 200
DPSYGSTVDL LDSKTFCRAQ ISMTTSSHLP VAESPDMKKE QDHPVKSHTK
210 220 230 240 250
KHNPRGTHLW EFIRDILLSP DKNPGLIKWE DRSEGIFRFL KSEAVAQLWG
260 270 280 290 300
KKKNNSSMTY EKLSRAMRYY YKREILERVD GRRLVYKFGK NARGWRENEN
Length:300
Mass (Da):34,903
Last modified:August 1, 1998 - v1
Checksum:i91D9477EFB874747
GO
Isoform 21 Publication (identifier: O70273-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     79-93: Missing.

Note: No experimental confirmation available.Curated

Show »
Length:285
Mass (Da):33,225
Checksum:i91398C527C65955F
GO
Isoform 31 Publication (identifier: O70273-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     200-211: KKHNPRGTHLWE → YFIIELLLSESR
     212-300: Missing.

Note: No experimental confirmation available.Curated

Show »
Length:211
Mass (Da):24,139
Checksum:i54F8846761D533CD
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei79 – 9315Missing in isoform 2. 1 PublicationVSP_052191Add
BLAST
Alternative sequencei200 – 21112KKHNP…THLWE → YFIIELLLSESR in isoform 3. 1 PublicationVSP_052192Add
BLAST
Alternative sequencei212 – 30089Missing in isoform 3. 1 PublicationVSP_052193Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF035527 mRNA. Translation: AAC40119.1.
BC005520 mRNA. Translation: AAH05520.1.
BC006789 mRNA. Translation: AAH06789.1.
BC008249 mRNA. Translation: AAH08249.1.
CCDSiCCDS16475.1. [O70273-1]
PIRiJW0048.
RefSeqiNP_031940.1. NM_007914.3. [O70273-1]
XP_006498760.1. XM_006498697.1. [O70273-1]
XP_006498761.1. XM_006498698.1. [O70273-1]
XP_006498762.1. XM_006498699.1. [O70273-1]
XP_006498763.1. XM_006498700.1. [O70273-1]
XP_006498764.1. XM_006498701.1. [O70273-1]
XP_006498765.1. XM_006498702.1. [O70273-1]
XP_006500821.1. XM_006500758.1. [O70273-1]
XP_006500822.1. XM_006500759.1. [O70273-1]
XP_006500823.1. XM_006500760.1. [O70273-1]
XP_006500824.1. XM_006500761.1. [O70273-1]
XP_006500825.1. XM_006500762.1. [O70273-1]
XP_006500826.1. XM_006500763.1. [O70273-1]
UniGeneiMm.10724.

Genome annotation databases

EnsembliENSMUST00000090475; ENSMUSP00000087961; ENSMUSG00000012350. [O70273-1]
ENSMUST00000111174; ENSMUSP00000106805; ENSMUSG00000012350. [O70273-1]
GeneIDi13661.
KEGGimmu:13661.
UCSCiuc008lio.1. mouse. [O70273-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF035527 mRNA. Translation: AAC40119.1.
BC005520 mRNA. Translation: AAH05520.1.
BC006789 mRNA. Translation: AAH06789.1.
BC008249 mRNA. Translation: AAH08249.1.
CCDSiCCDS16475.1. [O70273-1]
PIRiJW0048.
RefSeqiNP_031940.1. NM_007914.3. [O70273-1]
XP_006498760.1. XM_006498697.1. [O70273-1]
XP_006498761.1. XM_006498698.1. [O70273-1]
XP_006498762.1. XM_006498699.1. [O70273-1]
XP_006498763.1. XM_006498700.1. [O70273-1]
XP_006498764.1. XM_006498701.1. [O70273-1]
XP_006498765.1. XM_006498702.1. [O70273-1]
XP_006500821.1. XM_006500758.1. [O70273-1]
XP_006500822.1. XM_006500759.1. [O70273-1]
XP_006500823.1. XM_006500760.1. [O70273-1]
XP_006500824.1. XM_006500761.1. [O70273-1]
XP_006500825.1. XM_006500762.1. [O70273-1]
XP_006500826.1. XM_006500763.1. [O70273-1]
UniGeneiMm.10724.

3D structure databases

ProteinModelPortaliO70273.
SMRiO70273. Positions 40-111, 206-299.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199409. 1 interaction.

PTM databases

PhosphoSiteiO70273.

Proteomic databases

MaxQBiO70273.
PRIDEiO70273.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000090475; ENSMUSP00000087961; ENSMUSG00000012350. [O70273-1]
ENSMUST00000111174; ENSMUSP00000106805; ENSMUSG00000012350. [O70273-1]
GeneIDi13661.
KEGGimmu:13661.
UCSCiuc008lio.1. mouse. [O70273-1]

Organism-specific databases

CTDi26298.
MGIiMGI:1270840. Ehf.

Phylogenomic databases

eggNOGiNOG307806.
GeneTreeiENSGT00760000118996.
HOGENOMiHOG000232188.
HOVERGENiHBG074143.
InParanoidiO70273.
KOiK17102.
OMAiDENYLYD.
OrthoDBiEOG7M0NRR.
PhylomeDBiO70273.
TreeFamiTF318679.

Miscellaneous databases

NextBioi284404.
PROiO70273.
SOURCEiSearch...

Gene expression databases

BgeeiO70273.
CleanExiMM_EHF.
ExpressionAtlasiO70273. baseline and differential.
GenevestigatoriO70273.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.50. 1 hit.
InterProiIPR000418. Ets_dom.
IPR003118. Pointed_dom.
IPR013761. SAM/pointed.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and expression of Ehf, a new member of the ets transcription factor/oncoprotein gene family."
    Bochert M.A., Kleinbaum L.A., Sun L.-Y., Burton F.H.
    Biochem. Biophys. Res. Commun. 246:176-181(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Tissue: PituitaryImported.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 222-300 (ISOFORMS 1/2).
    Strain: Czech IIImported and FVB/NImported.
    Tissue: Mammary glandImported.
  3. "Determination of the consensus DNA-binding sequence and a transcriptional activation domain for ESE-2."
    Choi Y.S., Sinha S.
    Biochem. J. 398:497-507(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN.

Entry informationi

Entry nameiEHF_MOUSE
AccessioniPrimary (citable) accession number: O70273
Secondary accession number(s): Q922E8, Q922T6, Q99K12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: August 1, 1998
Last modified: March 4, 2015
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.