Reviewed, UniProtKB/Swiss-Prot O70257 (STX7_RAT)

Names and origin

Protein names

Recommended name:
    Syntaxin-7

Gene names

Name:

Stx7

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be involved in protein trafficking from the plasma membrane to the early endosome (EE) as well as in homotypic fusion of endocytic organelles. Mediates the endocytic trafficking from early endosomes to late endosomes and lysosomes By similarity.
Subunit structure	Interacts with STX8. Forms a SNARE complex with VTI1B, STX8 and VAMP8 which functions in the homotypic fusion of late endosomes. Component of the SNARE complex composed of STX7, STX8, VAMP7 and VTI1B that is required for heterotypic fusion of late endosomes with lysosomes in liver cells. Interacts with VPS11, VPS16 and VPS18. Found in a complex with VAMP8 and VTI1B in the liver. Part of the SNARE core complex containing VAMP8, STX8 and VTI1B By similarity. Interacts with VPS33A.
Subcellular location	Early endosome membrane; Single-pass type IV membrane protein By similarity.
Tissue specificity	Detected in all tissues tested. Highest expression is found in kidney followed by lung, spleen, heart and brain. Lower expression, in skeletal muscle, liver and testis.
Sequence similarities	Belongs to the syntaxin family. Contains 1 t-SNARE coiled-coil homology domain.

Ontologies

Keywords
Cellular component	Endosome Membrane
Domain	Coiled coil Transmembrane
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	intracellular protein transport Inferred from electronic annotation. Source: InterPro synaptic vesicle exocytosis Traceable author statement. Source: RGD
Cellular component	SNARE complex Ref.4 Inferred from direct assay. Source: UniProtKB endosome Inferred from direct assay. Source: RGD integral to membrane Inferred from direct assay. Source: RGD
Molecular function	SNAP receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 261	260	Syntaxin-7		PRO_0000210216
Regions
	Topological domain	2 – 238	237	Cytoplasmic Potential
	Transmembrane	239 – 259	21	Anchor for type IV membrane protein Potential
	Topological domain	260 – 261	2	Vesicular Potential
	Domain	165 – 227	63	t-SNARE coiled-coil homology
	Coiled coil	47 – 68	22	Potential
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	79	1	Phosphothreonine By similarity
	Modified residue	125	1	Phosphoserine By similarity
	Modified residue	126	1	Phosphoserine By similarity
	Modified residue	129	1	Phosphoserine By similarity
	Modified residue	205	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	139	1	E → A in AAC17131. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O70257-1 [UniParc]. Last modified May 1, 2007. Version 4. Checksum: 42D23BA7EC5F5F09	FASTA	261	29,851
	10 20 30 40 50 60 MSYTPGIGGD PAQLAQRISS NIQKITQCSA EIQRTLNQLG TPQDTPELRQ QLQQEQQYTN 70 80 90 100 110 120 QLAKETDKYI KEFGFLPTTP SEQRQRKIQK DRLVAEFTTA LTNFQKVQRQ AAEREKEFVA 130 140 150 160 170 180 RVRASSRVSG GFPEDSSKEK NFVSWESQTQ PQVQVQDEEI TEDDLRLIHE RESSIRQLEA 190 200 210 220 230 240 DIMDINEIFK DLGMMIHEQG DVIDSIEANV ESAEVHVQQA NQQLSRAANY QRKSRKTLCI 250 260 IILILVVGIV IIFFIVWGLK G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Syntaxin 7, a novel syntaxin member associated with the early endosomal compartment." Wong S.H., Xu Y., Zhang T., Hong W. J. Biol. Chem. 273:375-380(1998) [PubMed: 9417091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. Tissue: Brain.
[2]	"A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function." Antonin W., Holroyd C., Fasshauer D., Pabst S., Fischer von Mollard G., Jahn R. EMBO J. 19:6453-6464(2000) [PubMed: 11101518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SNARE COMPLEX CHARACTERIZATION.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.
[4]	"Combinatorial SNARE complexes with VAMP7 or VAMP8 define different late endocytic fusion events." Pryor P.R., Mullock B.M., Bright N.A., Lindsay M.R., Gray S.R., Richardson S.C.W., Stewart A., James D.E., Piper R.C., Luzio J.P. EMBO Rep. 5:590-595(2004) [PubMed: 15133481] [Abstract] Cited for: SNARE COMPLEX CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF031430 mRNA. Translation: AAC17131.1. BC085737 mRNA. Translation: AAH85737.1.
IPI	IPI00196648.
RefSeq	NP_068641.2.
UniGene	Rn.1993
3D structure databases
HSSP	HSSP built from PDB template 1JTH based on UniProtKB P32851.
SMR	O70257. Positions 169-227.
ModBase	Search...
Protein-protein interaction databases
STRING	O70257.
PTM databases
PhosphoSite	O70257.
Genome annotation databases
Ensembl	ENSRNOT00000021318; ENSRNOP00000021318; ENSRNOG00000015670; Rattus norvegicus. [Genome view]
GeneID	60466.
KEGG	rno:60466.
NMPDR	fig|10116.3.peg.252.
UCSC	NM_021869. rat.
Organism-specific databases
CTD	60466.
RGD	619747. Stx7.
Phylogenomic databases
HOVERGEN	O70257.
OMA	HEQGDMI.
Gene expression databases
ArrayExpress	O70257.
Genevestigator	O70257.
GermOnline	ENSRNOG00000015670. Rattus norvegicus.
Family and domain databases
InterPro	IPR006012. Syntaxin/epimorphin_CS. IPR006011. Syntaxin_N. IPR000727. T_SNARE. [Graphical view]
Pfam	PF05739. SNARE. 1 hit. PF00804. Syntaxin. 1 hit. [Graphical view]
SMART	SM00503. SynN. 1 hit. SM00397. t_SNARE. 1 hit. [Graphical view]
PROSITE	PS00914. SYNTAXIN. 1 hit. PS50192. T_SNARE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	612234.

Entry information

Entry name

STX7_RAT

Accession

Primary (citable) accession number: O70257
Secondary accession number(s): Q5U337

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2007
Last modified:	November 3, 2009
This is version 78 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)