O70249 (OGG1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-glycosylase/DNA lyase

Including the following 2 domains:

1. 8-oxoguanine DNA glycosylase
   EC=3.2.2.-
2. DNA-(apurinic or apyrimidinic site) lyase
   Short name=AP lyase
   EC=4.2.99.18

Gene names

Name:	Ogg1
Synonyms:	Mmh, Ogh1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.
Catalytic activity	The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
Subcellular location	Nucleus › nucleoplasm By similarity. Nucleus speckle By similarity. Nucleus matrix By similarity. Note: Together with APEX1 is recruited to nuclear speckles in UVA-irradiated cells By similarity.
Sequence similarities	Belongs to the type-1 OGG1 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Molecular function	Glycosidase Hydrolase Lyase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	acute inflammatory response Inferred from expression pattern PubMed 12853071. Source: RGD base-excision repair Inferred from direct assay Ref.1. Source: RGD cellular response to cadmium ion Inferred from expression pattern PubMed 12499121. Source: RGD nucleotide-excision repair Inferred from electronic annotation. Source: InterPro regulation of protein import into nucleus, translocation Inferred from sequence or structural similarity. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB response to drug Inferred from expression pattern PubMed 11872643PubMed 15031674. Source: RGD response to estradiol stimulus Inferred from direct assay PubMed 16182450. Source: RGD response to ethanol Inferred from expression pattern PubMed 11524300. Source: RGD response to folic acid Inferred from expression pattern PubMed 20051376. Source: RGD response to oxidative stress Inferred from sequence or structural similarity. Source: UniProtKB response to radiation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	mitochondrion Inferred from direct assay PubMed 12060578. Source: RGD nuclear matrix Inferred from sequence or structural similarity. Source: UniProtKB nuclear speck Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	8-oxo-7,8-dihydroguanine DNA N-glycosylase activity Inferred from electronic annotation. Source: Compara damaged DNA binding Inferred from direct assay Ref.1. Source: RGD oxidized purine nucleobase lesion DNA N-glycosylase activity Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	N-glycosylase/DNA lyase		PRO_0000058593
Sites
	Active site	249	1	Schiff-base intermediate with DNA By similarity
	Binding site	149	1	DNA By similarity
	Binding site	154	1	DNA By similarity
	Binding site	204	1	DNA By similarity
	Binding site	266	1	8-oxoguanine; via carbonyl oxygen By similarity
	Binding site	268	1	8-oxoguanine By similarity
	Binding site	270	1	DNA By similarity
	Binding site	287	1	DNA By similarity
	Binding site	315	1	8-oxoguanine By similarity
	Binding site	319	1	8-oxoguanine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O70249 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: B7FDF8C782644C41	FASTA	345	38,711
	10 20 30 40 50 60 MLFSSSLSSS MRHRTLTSSP ALWASIPCPR SELRLDLVLA SGQSFRWREQ SPAHWSGVLA 70 80 90 100 110 120 DQVWTLTQTE DQLYCTVYRG DKGQVGRPTL EELETLHKYF QLDVSLTQLY SHWASVDSHF 130 140 150 160 170 180 QSVAQKFQGV RLLRQDPTEC LFSFICSSNN NIARITGMVE RLCQAFGPRL VQLDDVTYHG 190 200 210 220 230 240 FPNLHALAGP EVETHLRKLG LGYRARYVCA SAKAILEEQG GPAWLQQLRV ASYEEAHKAL 250 260 270 280 290 300 CTLPGVGTKV ADCICLMALD KPQAVPVDIH VWQIAHRDYG WQPKTSQTKG PSPLANKELG 310 320 330 340 NFFRNLWGPY AGWAQAVLFS ADLRQQNLSR EPPAKRKKGS KKTEG

References

[1]	"Rat 7,8-dihydro-8-oxoguanine DNA glycosylase: substrate specificity, kinetics and cleavagemechanism at an apurinic site." Prieto Alamo M.J., Jurado J., Francastel E., Laval F. Nucleic Acids Res. 26:5199-5202(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Hepatoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF029690 mRNA. Translation: AAC77525.1.
IPI	IPI00196624.
RefSeq	NP_110497.1. NM_030870.1.
UniGene	Rn.22623.
3D structure databases
ProteinModelPortal	O70249.
SMR	O70249. Positions 13-323.
ModBase	Search...
PTM databases
PhosphoSite	O70249.
Proteomic databases
PRIDE	O70249.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	81528.
KEGG	rno:81528.
Organism-specific databases
CTD	4968.
RGD	621168. Ogg1.
Phylogenomic databases
eggNOG	COG0122.
HOGENOM	HOG000180756.
HOVERGEN	HBG001047.
InParanoid	O70249.
KO	K03660.
OrthoDB	EOG4P8FJN.
Gene expression databases
ArrayExpress	O70249.
Genevestigator	O70249.
Family and domain databases
Gene3D	1.10.1670.10. 1 hit. 1.10.340.30. 1 hit.
InterPro	IPR011257. DNA_glycosylase. IPR003265. HhH-GPD_domain. IPR023170. HTH_base_excis_C. IPR004577. Ogg. IPR012904. OGG_N. [Graphical view]
Pfam	PF00730. HhH-GPD. 1 hit. PF07934. OGG_N. 1 hit. [Graphical view]
SMART	SM00478. ENDO3c. 1 hit. [Graphical view]
SUPFAM	SSF48150. DNA_glycsylse. 1 hit.
TIGRFAMs	TIGR00588. ogg. 1 hit.
ProtoNet	Search...
Other
NextBio	615069.

Entry information

Entry name

OGG1_RAT

Accession

Primary (citable) accession number: O70249

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	August 1, 1998
Last modified:	April 3, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program