Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Amyloid beta A4 precursor protein-binding family A member 3

Gene

Apba3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May modulate processing of the beta-amyloid precursor protein (APP) and hence formation of beta-APP. May enhance the activity of HIF1A in macrophages by inhibiting the activity of HIF1AN (By similarity).By similarity

GO - Molecular functioni

  • beta-amyloid binding Source: RGD
  • enzyme inhibitor activity Source: Ensembl

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Amyloid beta A4 precursor protein-binding family A member 3
Alternative name(s):
Adapter protein X11gamma
Neuron-specific X11L2 protein
Neuronal Munc18-1-interacting protein 3
Short name:
Mint-3
Gene namesi
Name:Apba3
Synonyms:Mint3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi620846. Apba3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 569569Amyloid beta A4 precursor protein-binding family A member 3PRO_0000064622Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei166 – 1661PhosphoserineCombined sources
Modified residuei367 – 3671PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiO70248.
PRIDEiO70248.

PTM databases

PhosphoSiteiO70248.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

GenevisibleiO70248. RN.

Interactioni

Subunit structurei

Binds to the cytoplasmic domain of amyloid protein (APP). Interacts with HIF1AN (via N-terminus) (By similarity).By similarity

Protein-protein interaction databases

BioGridi249776. 1 interaction.
MINTiMINT-245193.
STRINGi10116.ENSRNOP00000027784.

Structurei

3D structure databases

ProteinModelPortaliO70248.
SMRiO70248. Positions 388-569.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini212 – 376165PIDPROSITE-ProRule annotationAdd
BLAST
Domaini389 – 47587PDZ 1PROSITE-ProRule annotationAdd
BLAST
Domaini480 – 55475PDZ 2PROSITE-ProRule annotationAdd
BLAST

Domaini

Composed of an N-terminal domain, a middle phosphotyrosine-binding domain (PID/PTB) that mediates binding with the cytoplasmic domain of the beta-amyloid precursor protein, and two C-terminal PDZ domains thought to attach proteins to the plasma membrane.

Sequence similaritiesi

Contains 2 PDZ (DHR) domains.PROSITE-ProRule annotation
Contains 1 PID domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3605. Eukaryota.
ENOG410XSBD. LUCA.
GeneTreeiENSGT00390000013578.
HOGENOMiHOG000033981.
HOVERGENiHBG050523.
InParanoidiO70248.
OMAiPGPCDHE.
OrthoDBiEOG78PV8H.
TreeFamiTF315245.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
2.30.42.10. 2 hits.
InterProiIPR030531. Apba3.
IPR001478. PDZ.
IPR011993. PH_dom-like.
IPR006020. PTB/PI_dom.
[Graphical view]
PANTHERiPTHR12345:SF9. PTHR12345:SF9. 1 hit.
PfamiPF00595. PDZ. 2 hits.
PF00640. PID. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 2 hits.
SM00462. PTB. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50106. PDZ. 2 hits.
PS01179. PID. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O70248-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFLPEPQHP PGPPTMDLEE PKGPEVPSED HPSNTQWALG PRGPDTLSEM
60 70 80 90 100
ELDTSSVRAL VQQLEALPSD LGGQFPDGAP CPLHIATGQG LATQENLDAG
110 120 130 140 150
GLLSAEAGGD NLLGLLRCEA SLPAQSVPPD PAQAAPRLLQ PPEDPGGDPG
160 170 180 190 200
WMEGTEPADN RSSSSSPELW LETAPLVTHR DPPVGTQSQE TLASCPAVSE
210 220 230 240 250
VPGPCGQEEL MDGVLFGAKY LGSTQLLSER NPPPSTRMGQ AQEAMDRVKA
260 270 280 290 300
PEGETQPMTE VDIFISTKRV KVLAADSQDA LMDHALQTIS YIADIGPVLV
310 320 330 340 350
LMARRRLAKR TTSQDRQRQL YKMLCHVFHS EDAQLIAQAI GQAFSIAYSQ
360 370 380 390 400
FLQENRIDPS QVGMQPSASA SHPHNGDLDH FCNSQNCREV CIQKRPGEGL
410 420 430 440 450
GVALVESGWG SLLPTAVIAN LLHGGPAERC GALSIGDRVT AINGTSLVGL
460 470 480 490 500
SLAACQAAVR EVRRHSSVTL SIIHCPPVTT AVIHRPHVRE QLGFCVENGI
510 520 530 540 550
ICSLLRGSAA ERGGVRVGHR IIEVNGQSVV AMPHARIIQL LTETREIHIK
560
TMPAATYRLL TGQEQPVYL
Length:569
Mass (Da):60,882
Last modified:April 18, 2012 - v2
Checksum:iA3DA45F4B2BBB338
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti37 – 371W → G in AAC17978 (PubMed:9860131).Curated
Sequence conflicti255 – 2551T → S in AAC17978 (PubMed:9860131).Curated
Sequence conflicti356 – 3561R → G in AAC17978 (PubMed:9860131).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF029109 mRNA. Translation: AAC17978.1.
CH474029 Genomic DNA. Translation: EDL89170.1.
BC161842 mRNA. Translation: AAI61842.1.
RefSeqiNP_113969.1. NM_031781.1.
XP_006241082.1. XM_006241020.2.
UniGeneiRn.6924.

Genome annotation databases

EnsembliENSRNOT00000027784; ENSRNOP00000027784; ENSRNOG00000020466.
GeneIDi83611.
KEGGirno:83611.
UCSCiRGD:620846. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF029109 mRNA. Translation: AAC17978.1.
CH474029 Genomic DNA. Translation: EDL89170.1.
BC161842 mRNA. Translation: AAI61842.1.
RefSeqiNP_113969.1. NM_031781.1.
XP_006241082.1. XM_006241020.2.
UniGeneiRn.6924.

3D structure databases

ProteinModelPortaliO70248.
SMRiO70248. Positions 388-569.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249776. 1 interaction.
MINTiMINT-245193.
STRINGi10116.ENSRNOP00000027784.

PTM databases

PhosphoSiteiO70248.

Proteomic databases

PaxDbiO70248.
PRIDEiO70248.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000027784; ENSRNOP00000027784; ENSRNOG00000020466.
GeneIDi83611.
KEGGirno:83611.
UCSCiRGD:620846. rat.

Organism-specific databases

CTDi9546.
RGDi620846. Apba3.

Phylogenomic databases

eggNOGiKOG3605. Eukaryota.
ENOG410XSBD. LUCA.
GeneTreeiENSGT00390000013578.
HOGENOMiHOG000033981.
HOVERGENiHBG050523.
InParanoidiO70248.
OMAiPGPCDHE.
OrthoDBiEOG78PV8H.
TreeFamiTF315245.

Miscellaneous databases

NextBioi616147.
PROiO70248.

Gene expression databases

GenevisibleiO70248. RN.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
2.30.42.10. 2 hits.
InterProiIPR030531. Apba3.
IPR001478. PDZ.
IPR011993. PH_dom-like.
IPR006020. PTB/PI_dom.
[Graphical view]
PANTHERiPTHR12345:SF9. PTHR12345:SF9. 1 hit.
PfamiPF00595. PDZ. 2 hits.
PF00640. PID. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 2 hits.
SM00462. PTB. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50106. PDZ. 2 hits.
PS01179. PID. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mint 3: a ubiquitous mint isoform that does not bind to munc18-1 or -2."
    Okamoto M., Suedhof T.C.
    Eur. J. Cell Biol. 77:161-165(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Brown Norway/Mcwi.
    Tissue: Embryonic spleen.
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-166, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAPBA3_RAT
AccessioniPrimary (citable) accession number: O70248
Secondary accession number(s): B1WBQ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: April 18, 2012
Last modified: May 11, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.