O70240 (AXIN2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Axin-2

Alternative name(s):
Axin-like protein
Short name=Axil
Axis inhibition protein 2
Conductin

Gene names

Name:

Axin2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	838 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B By similarity.
Subunit structure	Interacts with SMAD7 and RNF111. Interacts with ANKRD6 By similarity. Interacts with glycogen synthase kinase-3 beta (GSK3B) and beta-catenin. The interaction between axin and beta-catenin occurs via the armadillo repeats contained in beta-catenin. Ref.1
Subcellular location	Cytoplasm.
Tissue specificity	Expressed in lung and thymus. Ref.1
Domain	The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2 By similarity.
Post-translational modification	Probably phosphorylated by GSK3B and dephosphorylated by PP2A. ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway By similarity. Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription By similarity.
Sequence similarities	Contains 1 DIX domain. Contains 1 RGS domain.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Cytoplasm
Molecular function	Developmental protein
PTM	ADP-ribosylation Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway involved in somitogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome bone mineralization Inferred from electronic annotation. Source: Compara cell death Inferred from direct assay PubMed 15572025. Source: RGD cell proliferation Inferred from electronic annotation. Source: Compara cellular protein localization Inferred from electronic annotation. Source: Compara cellular response to organic cyclic compound Inferred from expression pattern PubMed 19464575. Source: RGD chondrocyte differentiation involved in endochondral bone morphogenesis Inferred from electronic annotation. Source: Compara intramembranous ossification Inferred from Biological aspect of Ancestor. Source: RefGenome mRNA stabilization Inferred from electronic annotation. Source: Compara maintenance of DNA repeat elements Inferred from electronic annotation. Source: Compara negative regulation of Wnt receptor signaling pathway involved in dorsal/ventral axis specification Inferred from direct assay Ref.1. Source: RGD negative regulation of canonical Wnt receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of catenin import into nucleus Inferred from electronic annotation. Source: Compara negative regulation of cell proliferation Inferred from direct assay PubMed 15572025. Source: RGD negative regulation of osteoblast differentiation Inferred from Biological aspect of Ancestor. Source: RefGenome odontogenesis Inferred from electronic annotation. Source: Compara positive regulation of cell death Inferred from electronic annotation. Source: Compara positive regulation of epithelial to mesenchymal transition Inferred from electronic annotation. Source: Compara positive regulation of protein kinase activity Inferred from direct assay Ref.1. Source: RGD regulation of catenin import into nucleus Inferred from Biological aspect of Ancestor. Source: RefGenome regulation of centromeric sister chromatid cohesion Inferred from electronic annotation. Source: Compara regulation of chondrocyte development Inferred from electronic annotation. Source: Compara regulation of mismatch repair Inferred from electronic annotation. Source: Compara termination of G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular_component	cell cortex Inferred from Biological aspect of Ancestor. Source: RefGenome centrosome Inferred from electronic annotation. Source: Compara cytoplasmic membrane-bounded vesicle Inferred from Biological aspect of Ancestor. Source: RefGenome cytoplasmic microtubule Inferred from Biological aspect of Ancestor. Source: RefGenome nucleus Inferred from electronic annotation. Source: Compara plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome postsynaptic density Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	GTPase activator activity Inferred from Biological aspect of Ancestor. Source: RefGenome armadillo repeat domain binding Inferred from Biological aspect of Ancestor. Source: RefGenome beta-catenin binding Inferred from direct assay Ref.1. Source: RGD signal transducer activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 838	838	Axin-2		PRO_0000220897
Regions
	Domain	81 – 200	120	RGS
	Domain	756 – 838	83	DIX
	Region	327 – 413	87	Interaction with GSK3B By similarity
	Region	413 – 476	64	Interaction with beta-catenin By similarity
	Motif	21 – 30	10	Tankyrase-binding motif
	Compositional bias	469 – 474	6	Poly-His
Amino acid modifications
	Modified residue	460	1	Phosphotyrosine By similarity
	Modified residue	464	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O70240 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 45B825C13BA07F37	FASTA	838	92,948
	10 20 30 40 50 60 MSSAVLVTLL PDPSSSFRED APRPPVPGEE GETPPCQPSV GKVQSTKPMP VSSNARRNED 70 80 90 100 110 120 GLGEPEGRAS PDSPLTRWTK SLHSLLGDQD GAYLFRTFLE REKCVDTLDF WFACNGFRQM 130 140 150 160 170 180 NLKDTKTLRV AKAIYKRYIE NNSVVSKQLK PATKTYIRDG IKKQQIGSVM FDQAQTEIQA 190 200 210 220 230 240 VMEENAYQVF LTSDIYLEYV RSGGENTAYM SNGGLGSLKV LCGYLPTLNE EEEWTCADLK 250 260 270 280 290 300 CKLSPTVVGL SSKTLRATAS VRSTETAENG FRSFKRSEPV NPYHVGSGYV FAPATSANDS 310 320 330 340 350 360 ELSSDALTDD SMSMTDSSVD GIPPYRMGSK KQLQREMHRS VKANGQVSLP HFPRTHRLPK 370 380 390 400 410 420 EMTPVEPAAF AAELISRLEK LKLELESRHS LEERLQQIRE DEEKEGSEQA LSSRDGAPVQ 430 440 450 460 470 480 HPLALLPSGS YEEDPQTILD DHLSRVLKTP GCQSPGVGRY SPRSRSPDHH HHHHQQCHAL 490 500 510 520 530 540 LPTGGKLPPE AACPLLGGKS FLTKQTTKHV HHHYIHHHAV PKTKEEIEAE ATQRVRCLCP 550 560 570 580 590 600 GGTDYYCYSK CKSHSKPPEP LPGEQFCGSR GGTLPKRNTK GTEPGLALPA REGGMSSAAG 610 620 630 640 650 660 APQLPGEEGD RSQDVWQWML ESERQSKSKP HSTQSIRKSY PLESARAPPG ERVSRHHLLG 670 680 690 700 710 720 ASGHPRSAAR AHPFTQDPAM PPLTPPNTLA QLEEACRRLA EVSKPQKQRC CVASQQRDRN 730 740 750 760 770 780 HPATGQAGPT SFSNPSLASE DHKEPKRLAS VHALQASELI VTYFFCGEEI PYRRMLKAQS 790 800 810 820 830 LTLGHFKEQL SKKGNYRYYF KKASDEFACG AVFEEIWDDE TVLPMYEGRI LGKVERID

References

[1]

"Axil, a member of the Axin family, interacts with both glycogen synthase kinase 3beta and beta-catenin and inhibits axis formation of Xenopus embryos." Yamamoto H., Kishida S., Uochi T., Ikeda S., Koyama S., Asashima M., Kikuchi A. Mol. Cell. Biol. 18:2867-2875(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CTNNB1 AND GSK3B, TISSUE SPECIFICITY. Tissue: Brain.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF017757 mRNA. Translation: AAC40089.1.
IPI	IPI00196619.
PIR	T08423.
RefSeq	NP_077331.1. NM_024355.1.
UniGene	Rn.162212.
3D structure databases
ProteinModelPortal	O70240.
SMR	O70240. Positions 67-206, 759-838.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-1534850.
STRING	10116.ENSRNOP00000004898.
Proteomic databases
PaxDb	O70240.
PRIDE	O70240.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	29134.
KEGG	rno:29134.
Organism-specific databases
CTD	8313.
RGD	69259. Axin2.
Phylogenomic databases
eggNOG	NOG238205.
HOVERGEN	HBG004324.
InParanoid	O70240.
KO	K04385.
OMA	NGYRSFK.
OrthoDB	EOG4X3H11.
Gene expression databases
ArrayExpress	O70240.
Genevestigator	O70240.
GermOnline	ENSRNOG00000003612. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.196.10. 2 hits.
InterPro	IPR014936. Axin_b-cat-bd. IPR001158. DIX. IPR000342. Regulat_G_prot_signal. IPR024066. Regulat_G_prot_signal_dom1. IPR016137. Regulat_G_prot_signal_superfam. [Graphical view]
Pfam	PF08833. Axin_b-cat_bind. 1 hit. PF00778. DIX. 1 hit. PF00615. RGS. 1 hit. [Graphical view]
PRINTS	PR01301. RGSPROTEIN.
SMART	SM00021. DAX. 1 hit. SM00315. RGS. 1 hit. [Graphical view]
SUPFAM	SSF48097. Regulat_G_prot_signal_superfam. 1 hit.
PROSITE	PS50841. DIX. 1 hit. PS50132. RGS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	608087.

Entry information

Entry name

AXIN2_RAT

Accession

Primary (citable) accession number: O70240

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	August 1, 1998
Last modified:	May 29, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program