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Protein

Axin-2

Gene

Axin2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B (By similarity).By similarity

GO - Molecular functioni

  1. armadillo repeat domain binding Source: GO_Central
  2. beta-catenin binding Source: RGD
  3. GTPase activator activity Source: GO_Central
  4. protein kinase binding Source: RGD

GO - Biological processi

  1. cell death Source: RGD
  2. cellular response to organic cyclic compound Source: RGD
  3. intramembranous ossification Source: GO_Central
  4. negative regulation of canonical Wnt signaling pathway Source: GO_Central
  5. negative regulation of cell proliferation Source: RGD
  6. negative regulation of osteoblast differentiation Source: GO_Central
  7. negative regulation of Wnt signaling pathway involved in dorsal/ventral axis specification Source: RGD
  8. positive regulation of GTPase activity Source: GOC
  9. positive regulation of protein kinase activity Source: RGD
  10. regulation of catenin import into nucleus Source: GO_Central
  11. termination of G-protein coupled receptor signaling pathway Source: InterPro
  12. Wnt signaling pathway involved in somitogenesis Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Axin-2
Alternative name(s):
Axin-like protein
Short name:
Axil
Axis inhibition protein 2
Conductin
Gene namesi
Name:Axin2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69259. Axin2.

Subcellular locationi

GO - Cellular componenti

  1. cell cortex Source: GO_Central
  2. cytoplasmic membrane-bounded vesicle Source: GO_Central
  3. cytoplasmic microtubule Source: GO_Central
  4. plasma membrane Source: GO_Central
  5. postsynaptic density Source: GO_Central
  6. protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 838838Axin-2PRO_0000220897Add
BLAST

Post-translational modificationi

Probably phosphorylated by GSK3B and dephosphorylated by PP2A.
ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway (By similarity).By similarity
Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription (By similarity).By similarity

Keywords - PTMi

ADP-ribosylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO70240.
PRIDEiO70240.

Expressioni

Tissue specificityi

Expressed in lung and thymus.1 Publication

Gene expression databases

GenevestigatoriO70240.

Interactioni

Subunit structurei

Interacts with SMAD7 and RNF111. Interacts with ANKRD6 (By similarity). Interacts with glycogen synthase kinase-3 beta (GSK3B) and beta-catenin. The interaction between axin and beta-catenin occurs via the armadillo repeats contained in beta-catenin.By similarity1 Publication

Protein-protein interaction databases

MINTiMINT-1534850.
STRINGi10116.ENSRNOP00000004898.

Structurei

3D structure databases

ProteinModelPortaliO70240.
SMRiO70240. Positions 67-206, 759-838.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini81 – 200120RGSPROSITE-ProRule annotationAdd
BLAST
Domaini756 – 83883DIXPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni327 – 41387Interaction with GSK3BBy similarityAdd
BLAST
Regioni413 – 47664Interaction with beta-cateninBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi21 – 3010Tankyrase-binding motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi469 – 4746Poly-His

Domaini

The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.By similarity

Sequence similaritiesi

Contains 1 DIX domain.PROSITE-ProRule annotation
Contains 1 RGS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG238205.
HOVERGENiHBG004324.
InParanoidiO70240.
KOiK04385.
OrthoDBiEOG79PJQ0.
PhylomeDBiO70240.
TreeFamiTF315454.

Family and domain databases

Gene3Di1.10.196.10. 2 hits.
InterProiIPR014936. Axin_b-cat-bd.
IPR001158. DIX.
IPR016137. RGS.
IPR024066. RGS_subdom1.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF08833. Axin_b-cat_bind. 1 hit.
PF00778. DIX. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00021. DAX. 1 hit.
SM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50841. DIX. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O70240-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSAVLVTLL PDPSSSFRED APRPPVPGEE GETPPCQPSV GKVQSTKPMP
60 70 80 90 100
VSSNARRNED GLGEPEGRAS PDSPLTRWTK SLHSLLGDQD GAYLFRTFLE
110 120 130 140 150
REKCVDTLDF WFACNGFRQM NLKDTKTLRV AKAIYKRYIE NNSVVSKQLK
160 170 180 190 200
PATKTYIRDG IKKQQIGSVM FDQAQTEIQA VMEENAYQVF LTSDIYLEYV
210 220 230 240 250
RSGGENTAYM SNGGLGSLKV LCGYLPTLNE EEEWTCADLK CKLSPTVVGL
260 270 280 290 300
SSKTLRATAS VRSTETAENG FRSFKRSEPV NPYHVGSGYV FAPATSANDS
310 320 330 340 350
ELSSDALTDD SMSMTDSSVD GIPPYRMGSK KQLQREMHRS VKANGQVSLP
360 370 380 390 400
HFPRTHRLPK EMTPVEPAAF AAELISRLEK LKLELESRHS LEERLQQIRE
410 420 430 440 450
DEEKEGSEQA LSSRDGAPVQ HPLALLPSGS YEEDPQTILD DHLSRVLKTP
460 470 480 490 500
GCQSPGVGRY SPRSRSPDHH HHHHQQCHAL LPTGGKLPPE AACPLLGGKS
510 520 530 540 550
FLTKQTTKHV HHHYIHHHAV PKTKEEIEAE ATQRVRCLCP GGTDYYCYSK
560 570 580 590 600
CKSHSKPPEP LPGEQFCGSR GGTLPKRNTK GTEPGLALPA REGGMSSAAG
610 620 630 640 650
APQLPGEEGD RSQDVWQWML ESERQSKSKP HSTQSIRKSY PLESARAPPG
660 670 680 690 700
ERVSRHHLLG ASGHPRSAAR AHPFTQDPAM PPLTPPNTLA QLEEACRRLA
710 720 730 740 750
EVSKPQKQRC CVASQQRDRN HPATGQAGPT SFSNPSLASE DHKEPKRLAS
760 770 780 790 800
VHALQASELI VTYFFCGEEI PYRRMLKAQS LTLGHFKEQL SKKGNYRYYF
810 820 830
KKASDEFACG AVFEEIWDDE TVLPMYEGRI LGKVERID
Length:838
Mass (Da):92,948
Last modified:August 1, 1998 - v1
Checksum:i45B825C13BA07F37
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017757 mRNA. Translation: AAC40089.1.
PIRiT08423.
RefSeqiNP_077331.1. NM_024355.1.
UniGeneiRn.162212.

Genome annotation databases

GeneIDi29134.
KEGGirno:29134.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017757 mRNA. Translation: AAC40089.1.
PIRiT08423.
RefSeqiNP_077331.1. NM_024355.1.
UniGeneiRn.162212.

3D structure databases

ProteinModelPortaliO70240.
SMRiO70240. Positions 67-206, 759-838.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1534850.
STRINGi10116.ENSRNOP00000004898.

Proteomic databases

PaxDbiO70240.
PRIDEiO70240.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29134.
KEGGirno:29134.

Organism-specific databases

CTDi8313.
RGDi69259. Axin2.

Phylogenomic databases

eggNOGiNOG238205.
HOVERGENiHBG004324.
InParanoidiO70240.
KOiK04385.
OrthoDBiEOG79PJQ0.
PhylomeDBiO70240.
TreeFamiTF315454.

Miscellaneous databases

NextBioi608087.
PROiO70240.

Gene expression databases

GenevestigatoriO70240.

Family and domain databases

Gene3Di1.10.196.10. 2 hits.
InterProiIPR014936. Axin_b-cat-bd.
IPR001158. DIX.
IPR016137. RGS.
IPR024066. RGS_subdom1.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF08833. Axin_b-cat_bind. 1 hit.
PF00778. DIX. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00021. DAX. 1 hit.
SM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50841. DIX. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Axil, a member of the Axin family, interacts with both glycogen synthase kinase 3beta and beta-catenin and inhibits axis formation of Xenopus embryos."
    Yamamoto H., Kishida S., Uochi T., Ikeda S., Koyama S., Asashima M., Kikuchi A.
    Mol. Cell. Biol. 18:2867-2875(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CTNNB1 AND GSK3B, TISSUE SPECIFICITY.
    Tissue: Brain.

Entry informationi

Entry nameiAXIN2_RAT
AccessioniPrimary (citable) accession number: O70240
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: February 4, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.