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O70240

- AXIN2_RAT

UniProt

O70240 - AXIN2_RAT

Protein

Axin-2

Gene

Axin2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B By similarity.By similarity

    GO - Molecular functioni

    1. armadillo repeat domain binding Source: RefGenome
    2. beta-catenin binding Source: RGD
    3. GTPase activator activity Source: RefGenome
    4. protein kinase binding Source: RGD
    5. signal transducer activity Source: InterPro

    GO - Biological processi

    1. cell death Source: RGD
    2. cellular response to organic cyclic compound Source: RGD
    3. intramembranous ossification Source: RefGenome
    4. negative regulation of canonical Wnt signaling pathway Source: RefGenome
    5. negative regulation of cell proliferation Source: RGD
    6. negative regulation of osteoblast differentiation Source: RefGenome
    7. negative regulation of Wnt signaling pathway involved in dorsal/ventral axis specification Source: RGD
    8. positive regulation of GTPase activity Source: GOC
    9. positive regulation of protein kinase activity Source: RGD
    10. regulation of catenin import into nucleus Source: RefGenome
    11. termination of G-protein coupled receptor signaling pathway Source: InterPro
    12. Wnt signaling pathway involved in somitogenesis Source: RefGenome

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Wnt signaling pathway

    Enzyme and pathway databases

    ReactomeiREACT_212065. TCF dependent signaling in response to WNT.
    REACT_227706. degradation of AXIN.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Axin-2
    Alternative name(s):
    Axin-like protein
    Short name:
    Axil
    Axis inhibition protein 2
    Conductin
    Gene namesi
    Name:Axin2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi69259. Axin2.

    Subcellular locationi

    GO - Cellular componenti

    1. cell cortex Source: RefGenome
    2. cytoplasmic membrane-bounded vesicle Source: RefGenome
    3. cytoplasmic microtubule Source: RefGenome
    4. plasma membrane Source: RefGenome
    5. postsynaptic density Source: RefGenome
    6. protein complex Source: RGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 838838Axin-2PRO_0000220897Add
    BLAST

    Post-translational modificationi

    Probably phosphorylated by GSK3B and dephosphorylated by PP2A.
    ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway By similarity.By similarity
    Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription By similarity.By similarity

    Keywords - PTMi

    ADP-ribosylation, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiO70240.
    PRIDEiO70240.

    Expressioni

    Tissue specificityi

    Expressed in lung and thymus.1 Publication

    Gene expression databases

    GenevestigatoriO70240.

    Interactioni

    Subunit structurei

    Interacts with SMAD7 and RNF111. Interacts with ANKRD6 By similarity. Interacts with glycogen synthase kinase-3 beta (GSK3B) and beta-catenin. The interaction between axin and beta-catenin occurs via the armadillo repeats contained in beta-catenin.By similarity1 Publication

    Protein-protein interaction databases

    MINTiMINT-1534850.
    STRINGi10116.ENSRNOP00000004898.

    Structurei

    3D structure databases

    ProteinModelPortaliO70240.
    SMRiO70240. Positions 67-206, 759-838.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini81 – 200120RGSPROSITE-ProRule annotationAdd
    BLAST
    Domaini756 – 83883DIXPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni327 – 41387Interaction with GSK3BBy similarityAdd
    BLAST
    Regioni413 – 47664Interaction with beta-cateninBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi21 – 3010Tankyrase-binding motif

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi469 – 4746Poly-His

    Domaini

    The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.By similarity

    Sequence similaritiesi

    Contains 1 DIX domain.PROSITE-ProRule annotation
    Contains 1 RGS domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG238205.
    HOVERGENiHBG004324.
    InParanoidiO70240.
    KOiK04385.
    OrthoDBiEOG79PJQ0.
    PhylomeDBiO70240.
    TreeFamiTF315454.

    Family and domain databases

    Gene3Di1.10.196.10. 2 hits.
    InterProiIPR014936. Axin_b-cat-bd.
    IPR001158. DIX.
    IPR024066. Regulat_G_prot_signal_dom1.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    PfamiPF08833. Axin_b-cat_bind. 1 hit.
    PF00778. DIX. 1 hit.
    PF00615. RGS. 1 hit.
    [Graphical view]
    PRINTSiPR01301. RGSPROTEIN.
    SMARTiSM00021. DAX. 1 hit.
    SM00315. RGS. 1 hit.
    [Graphical view]
    SUPFAMiSSF48097. SSF48097. 1 hit.
    SSF54236. SSF54236. 1 hit.
    PROSITEiPS50841. DIX. 1 hit.
    PS50132. RGS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O70240-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSAVLVTLL PDPSSSFRED APRPPVPGEE GETPPCQPSV GKVQSTKPMP    50
    VSSNARRNED GLGEPEGRAS PDSPLTRWTK SLHSLLGDQD GAYLFRTFLE 100
    REKCVDTLDF WFACNGFRQM NLKDTKTLRV AKAIYKRYIE NNSVVSKQLK 150
    PATKTYIRDG IKKQQIGSVM FDQAQTEIQA VMEENAYQVF LTSDIYLEYV 200
    RSGGENTAYM SNGGLGSLKV LCGYLPTLNE EEEWTCADLK CKLSPTVVGL 250
    SSKTLRATAS VRSTETAENG FRSFKRSEPV NPYHVGSGYV FAPATSANDS 300
    ELSSDALTDD SMSMTDSSVD GIPPYRMGSK KQLQREMHRS VKANGQVSLP 350
    HFPRTHRLPK EMTPVEPAAF AAELISRLEK LKLELESRHS LEERLQQIRE 400
    DEEKEGSEQA LSSRDGAPVQ HPLALLPSGS YEEDPQTILD DHLSRVLKTP 450
    GCQSPGVGRY SPRSRSPDHH HHHHQQCHAL LPTGGKLPPE AACPLLGGKS 500
    FLTKQTTKHV HHHYIHHHAV PKTKEEIEAE ATQRVRCLCP GGTDYYCYSK 550
    CKSHSKPPEP LPGEQFCGSR GGTLPKRNTK GTEPGLALPA REGGMSSAAG 600
    APQLPGEEGD RSQDVWQWML ESERQSKSKP HSTQSIRKSY PLESARAPPG 650
    ERVSRHHLLG ASGHPRSAAR AHPFTQDPAM PPLTPPNTLA QLEEACRRLA 700
    EVSKPQKQRC CVASQQRDRN HPATGQAGPT SFSNPSLASE DHKEPKRLAS 750
    VHALQASELI VTYFFCGEEI PYRRMLKAQS LTLGHFKEQL SKKGNYRYYF 800
    KKASDEFACG AVFEEIWDDE TVLPMYEGRI LGKVERID 838
    Length:838
    Mass (Da):92,948
    Last modified:August 1, 1998 - v1
    Checksum:i45B825C13BA07F37
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF017757 mRNA. Translation: AAC40089.1.
    PIRiT08423.
    RefSeqiNP_077331.1. NM_024355.1.
    UniGeneiRn.162212.

    Genome annotation databases

    GeneIDi29134.
    KEGGirno:29134.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF017757 mRNA. Translation: AAC40089.1 .
    PIRi T08423.
    RefSeqi NP_077331.1. NM_024355.1.
    UniGenei Rn.162212.

    3D structure databases

    ProteinModelPortali O70240.
    SMRi O70240. Positions 67-206, 759-838.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-1534850.
    STRINGi 10116.ENSRNOP00000004898.

    Proteomic databases

    PaxDbi O70240.
    PRIDEi O70240.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 29134.
    KEGGi rno:29134.

    Organism-specific databases

    CTDi 8313.
    RGDi 69259. Axin2.

    Phylogenomic databases

    eggNOGi NOG238205.
    HOVERGENi HBG004324.
    InParanoidi O70240.
    KOi K04385.
    OrthoDBi EOG79PJQ0.
    PhylomeDBi O70240.
    TreeFami TF315454.

    Enzyme and pathway databases

    Reactomei REACT_212065. TCF dependent signaling in response to WNT.
    REACT_227706. degradation of AXIN.

    Miscellaneous databases

    NextBioi 608087.
    PROi O70240.

    Gene expression databases

    Genevestigatori O70240.

    Family and domain databases

    Gene3Di 1.10.196.10. 2 hits.
    InterProi IPR014936. Axin_b-cat-bd.
    IPR001158. DIX.
    IPR024066. Regulat_G_prot_signal_dom1.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    Pfami PF08833. Axin_b-cat_bind. 1 hit.
    PF00778. DIX. 1 hit.
    PF00615. RGS. 1 hit.
    [Graphical view ]
    PRINTSi PR01301. RGSPROTEIN.
    SMARTi SM00021. DAX. 1 hit.
    SM00315. RGS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48097. SSF48097. 1 hit.
    SSF54236. SSF54236. 1 hit.
    PROSITEi PS50841. DIX. 1 hit.
    PS50132. RGS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Axil, a member of the Axin family, interacts with both glycogen synthase kinase 3beta and beta-catenin and inhibits axis formation of Xenopus embryos."
      Yamamoto H., Kishida S., Uochi T., Ikeda S., Koyama S., Asashima M., Kikuchi A.
      Mol. Cell. Biol. 18:2867-2875(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CTNNB1 AND GSK3B, TISSUE SPECIFICITY.
      Tissue: Brain.

    Entry informationi

    Entry nameiAXIN2_RAT
    AccessioniPrimary (citable) accession number: O70240
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3