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O70240

- AXIN2_RAT

UniProt

O70240 - AXIN2_RAT

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Protein
Axin-2
Gene
Axin2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B By similarity.

GO - Molecular functioni

  1. GTPase activator activity Source: RefGenome
  2. armadillo repeat domain binding Source: RefGenome
  3. beta-catenin binding Source: RGD
  4. protein kinase binding Source: RGD
  5. signal transducer activity Source: InterPro

GO - Biological processi

  1. Wnt signaling pathway involved in somitogenesis Source: RefGenome
  2. cell death Source: RGD
  3. cellular response to organic cyclic compound Source: RGD
  4. intramembranous ossification Source: RefGenome
  5. negative regulation of Wnt signaling pathway involved in dorsal/ventral axis specification Source: RGD
  6. negative regulation of canonical Wnt signaling pathway Source: RefGenome
  7. negative regulation of cell proliferation Source: RGD
  8. negative regulation of osteoblast differentiation Source: RefGenome
  9. positive regulation of GTPase activity Source: GOC
  10. positive regulation of protein kinase activity Source: RGD
  11. regulation of catenin import into nucleus Source: RefGenome
  12. termination of G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Wnt signaling pathway

Enzyme and pathway databases

ReactomeiREACT_212065. TCF dependent signaling in response to WNT.
REACT_227706. degradation of AXIN.

Names & Taxonomyi

Protein namesi
Recommended name:
Axin-2
Alternative name(s):
Axin-like protein
Short name:
Axil
Axis inhibition protein 2
Conductin
Gene namesi
Name:Axin2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi69259. Axin2.

Subcellular locationi

GO - Cellular componenti

  1. cell cortex Source: RefGenome
  2. cytoplasmic membrane-bounded vesicle Source: RefGenome
  3. cytoplasmic microtubule Source: RefGenome
  4. plasma membrane Source: RefGenome
  5. postsynaptic density Source: RefGenome
  6. protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 838838Axin-2
PRO_0000220897Add
BLAST

Post-translational modificationi

Probably phosphorylated by GSK3B and dephosphorylated by PP2A.
ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway By similarity.
Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription By similarity.

Keywords - PTMi

ADP-ribosylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO70240.
PRIDEiO70240.

Expressioni

Tissue specificityi

Expressed in lung and thymus.1 Publication

Gene expression databases

GenevestigatoriO70240.

Interactioni

Subunit structurei

Interacts with SMAD7 and RNF111. Interacts with ANKRD6 By similarity. Interacts with glycogen synthase kinase-3 beta (GSK3B) and beta-catenin. The interaction between axin and beta-catenin occurs via the armadillo repeats contained in beta-catenin.1 Publication

Protein-protein interaction databases

MINTiMINT-1534850.
STRINGi10116.ENSRNOP00000004898.

Structurei

3D structure databases

ProteinModelPortaliO70240.
SMRiO70240. Positions 67-206, 759-838.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini81 – 200120RGS
Add
BLAST
Domaini756 – 83883DIX
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni327 – 41387Interaction with GSK3B By similarity
Add
BLAST
Regioni413 – 47664Interaction with beta-catenin By similarity
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi21 – 3010Tankyrase-binding motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi469 – 4746Poly-His

Domaini

The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2 By similarity.

Sequence similaritiesi

Contains 1 DIX domain.
Contains 1 RGS domain.

Phylogenomic databases

eggNOGiNOG238205.
HOVERGENiHBG004324.
InParanoidiO70240.
KOiK04385.
OrthoDBiEOG79PJQ0.
PhylomeDBiO70240.
TreeFamiTF315454.

Family and domain databases

Gene3Di1.10.196.10. 2 hits.
InterProiIPR014936. Axin_b-cat-bd.
IPR001158. DIX.
IPR024066. Regulat_G_prot_signal_dom1.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF08833. Axin_b-cat_bind. 1 hit.
PF00778. DIX. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00021. DAX. 1 hit.
SM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50841. DIX. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O70240-1 [UniParc]FASTAAdd to Basket

« Hide

MSSAVLVTLL PDPSSSFRED APRPPVPGEE GETPPCQPSV GKVQSTKPMP    50
VSSNARRNED GLGEPEGRAS PDSPLTRWTK SLHSLLGDQD GAYLFRTFLE 100
REKCVDTLDF WFACNGFRQM NLKDTKTLRV AKAIYKRYIE NNSVVSKQLK 150
PATKTYIRDG IKKQQIGSVM FDQAQTEIQA VMEENAYQVF LTSDIYLEYV 200
RSGGENTAYM SNGGLGSLKV LCGYLPTLNE EEEWTCADLK CKLSPTVVGL 250
SSKTLRATAS VRSTETAENG FRSFKRSEPV NPYHVGSGYV FAPATSANDS 300
ELSSDALTDD SMSMTDSSVD GIPPYRMGSK KQLQREMHRS VKANGQVSLP 350
HFPRTHRLPK EMTPVEPAAF AAELISRLEK LKLELESRHS LEERLQQIRE 400
DEEKEGSEQA LSSRDGAPVQ HPLALLPSGS YEEDPQTILD DHLSRVLKTP 450
GCQSPGVGRY SPRSRSPDHH HHHHQQCHAL LPTGGKLPPE AACPLLGGKS 500
FLTKQTTKHV HHHYIHHHAV PKTKEEIEAE ATQRVRCLCP GGTDYYCYSK 550
CKSHSKPPEP LPGEQFCGSR GGTLPKRNTK GTEPGLALPA REGGMSSAAG 600
APQLPGEEGD RSQDVWQWML ESERQSKSKP HSTQSIRKSY PLESARAPPG 650
ERVSRHHLLG ASGHPRSAAR AHPFTQDPAM PPLTPPNTLA QLEEACRRLA 700
EVSKPQKQRC CVASQQRDRN HPATGQAGPT SFSNPSLASE DHKEPKRLAS 750
VHALQASELI VTYFFCGEEI PYRRMLKAQS LTLGHFKEQL SKKGNYRYYF 800
KKASDEFACG AVFEEIWDDE TVLPMYEGRI LGKVERID 838
Length:838
Mass (Da):92,948
Last modified:August 1, 1998 - v1
Checksum:i45B825C13BA07F37
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF017757 mRNA. Translation: AAC40089.1.
PIRiT08423.
RefSeqiNP_077331.1. NM_024355.1.
UniGeneiRn.162212.

Genome annotation databases

GeneIDi29134.
KEGGirno:29134.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF017757 mRNA. Translation: AAC40089.1 .
PIRi T08423.
RefSeqi NP_077331.1. NM_024355.1.
UniGenei Rn.162212.

3D structure databases

ProteinModelPortali O70240.
SMRi O70240. Positions 67-206, 759-838.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-1534850.
STRINGi 10116.ENSRNOP00000004898.

Proteomic databases

PaxDbi O70240.
PRIDEi O70240.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 29134.
KEGGi rno:29134.

Organism-specific databases

CTDi 8313.
RGDi 69259. Axin2.

Phylogenomic databases

eggNOGi NOG238205.
HOVERGENi HBG004324.
InParanoidi O70240.
KOi K04385.
OrthoDBi EOG79PJQ0.
PhylomeDBi O70240.
TreeFami TF315454.

Enzyme and pathway databases

Reactomei REACT_212065. TCF dependent signaling in response to WNT.
REACT_227706. degradation of AXIN.

Miscellaneous databases

NextBioi 608087.
PROi O70240.

Gene expression databases

Genevestigatori O70240.

Family and domain databases

Gene3Di 1.10.196.10. 2 hits.
InterProi IPR014936. Axin_b-cat-bd.
IPR001158. DIX.
IPR024066. Regulat_G_prot_signal_dom1.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view ]
Pfami PF08833. Axin_b-cat_bind. 1 hit.
PF00778. DIX. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view ]
PRINTSi PR01301. RGSPROTEIN.
SMARTi SM00021. DAX. 1 hit.
SM00315. RGS. 1 hit.
[Graphical view ]
SUPFAMi SSF48097. SSF48097. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEi PS50841. DIX. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Axil, a member of the Axin family, interacts with both glycogen synthase kinase 3beta and beta-catenin and inhibits axis formation of Xenopus embryos."
    Yamamoto H., Kishida S., Uochi T., Ikeda S., Koyama S., Asashima M., Kikuchi A.
    Mol. Cell. Biol. 18:2867-2875(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CTNNB1 AND GSK3B, TISSUE SPECIFICITY.
    Tissue: Brain.

Entry informationi

Entry nameiAXIN2_RAT
AccessioniPrimary (citable) accession number: O70240
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: September 3, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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