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O70240 (AXIN2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Axin-2
Alternative name(s):
Axin-like protein
Short name=Axil
Axis inhibition protein 2
Conductin
Gene names
Name:Axin2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length838 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B By similarity.

Subunit structure

Interacts with SMAD7 and RNF111. Interacts with ANKRD6 By similarity. Interacts with glycogen synthase kinase-3 beta (GSK3B) and beta-catenin. The interaction between axin and beta-catenin occurs via the armadillo repeats contained in beta-catenin. Ref.1

Subcellular location

Cytoplasm.

Tissue specificity

Expressed in lung and thymus. Ref.1

Domain

The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2 By similarity.

Post-translational modification

Probably phosphorylated by GSK3B and dephosphorylated by PP2A.

ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway By similarity.

Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription By similarity.

Sequence similarities

Contains 1 DIX domain.

Contains 1 RGS domain.

Ontologies

Keywords
   Biological processWnt signaling pathway
   Cellular componentCytoplasm
   Molecular functionDevelopmental protein
   PTMADP-ribosylation
Phosphoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processWnt signaling pathway involved in somitogenesis

Inferred from Biological aspect of Ancestor. Source: RefGenome

cell death

Inferred from direct assay PubMed 15572025. Source: RGD

cellular response to organic cyclic compound

Inferred from expression pattern PubMed 19464575. Source: RGD

intramembranous ossification

Inferred from Biological aspect of Ancestor. Source: RefGenome

negative regulation of Wnt signaling pathway involved in dorsal/ventral axis specification

Inferred from direct assay Ref.1. Source: RGD

negative regulation of canonical Wnt signaling pathway

Inferred from Biological aspect of Ancestor. Source: RefGenome

negative regulation of cell proliferation

Inferred from direct assay PubMed 15572025. Source: RGD

negative regulation of osteoblast differentiation

Inferred from Biological aspect of Ancestor. Source: RefGenome

positive regulation of GTPase activity

Inferred from Biological aspect of Ancestor. Source: GOC

positive regulation of protein kinase activity

Inferred from direct assay Ref.1. Source: RGD

regulation of catenin import into nucleus

Inferred from Biological aspect of Ancestor. Source: RefGenome

termination of G-protein coupled receptor signaling pathway

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcell cortex

Inferred from Biological aspect of Ancestor. Source: RefGenome

cytoplasmic membrane-bounded vesicle

Inferred from Biological aspect of Ancestor. Source: RefGenome

cytoplasmic microtubule

Inferred from Biological aspect of Ancestor. Source: RefGenome

plasma membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

postsynaptic density

Inferred from Biological aspect of Ancestor. Source: RefGenome

protein complex

Inferred from direct assay Ref.1. Source: RGD

   Molecular_functionGTPase activator activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

armadillo repeat domain binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

beta-catenin binding

Inferred from direct assay Ref.1. Source: RGD

protein kinase binding

Inferred from physical interaction Ref.1. Source: RGD

signal transducer activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 838838Axin-2
PRO_0000220897

Regions

Domain81 – 200120RGS
Domain756 – 83883DIX
Region327 – 41387Interaction with GSK3B By similarity
Region413 – 47664Interaction with beta-catenin By similarity
Motif21 – 3010Tankyrase-binding motif
Compositional bias469 – 4746Poly-His

Sequences

Sequence LengthMass (Da)Tools
O70240 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 45B825C13BA07F37

FASTA83892,948
        10         20         30         40         50         60 
MSSAVLVTLL PDPSSSFRED APRPPVPGEE GETPPCQPSV GKVQSTKPMP VSSNARRNED 

        70         80         90        100        110        120 
GLGEPEGRAS PDSPLTRWTK SLHSLLGDQD GAYLFRTFLE REKCVDTLDF WFACNGFRQM 

       130        140        150        160        170        180 
NLKDTKTLRV AKAIYKRYIE NNSVVSKQLK PATKTYIRDG IKKQQIGSVM FDQAQTEIQA 

       190        200        210        220        230        240 
VMEENAYQVF LTSDIYLEYV RSGGENTAYM SNGGLGSLKV LCGYLPTLNE EEEWTCADLK 

       250        260        270        280        290        300 
CKLSPTVVGL SSKTLRATAS VRSTETAENG FRSFKRSEPV NPYHVGSGYV FAPATSANDS 

       310        320        330        340        350        360 
ELSSDALTDD SMSMTDSSVD GIPPYRMGSK KQLQREMHRS VKANGQVSLP HFPRTHRLPK 

       370        380        390        400        410        420 
EMTPVEPAAF AAELISRLEK LKLELESRHS LEERLQQIRE DEEKEGSEQA LSSRDGAPVQ 

       430        440        450        460        470        480 
HPLALLPSGS YEEDPQTILD DHLSRVLKTP GCQSPGVGRY SPRSRSPDHH HHHHQQCHAL 

       490        500        510        520        530        540 
LPTGGKLPPE AACPLLGGKS FLTKQTTKHV HHHYIHHHAV PKTKEEIEAE ATQRVRCLCP 

       550        560        570        580        590        600 
GGTDYYCYSK CKSHSKPPEP LPGEQFCGSR GGTLPKRNTK GTEPGLALPA REGGMSSAAG 

       610        620        630        640        650        660 
APQLPGEEGD RSQDVWQWML ESERQSKSKP HSTQSIRKSY PLESARAPPG ERVSRHHLLG 

       670        680        690        700        710        720 
ASGHPRSAAR AHPFTQDPAM PPLTPPNTLA QLEEACRRLA EVSKPQKQRC CVASQQRDRN 

       730        740        750        760        770        780 
HPATGQAGPT SFSNPSLASE DHKEPKRLAS VHALQASELI VTYFFCGEEI PYRRMLKAQS 

       790        800        810        820        830 
LTLGHFKEQL SKKGNYRYYF KKASDEFACG AVFEEIWDDE TVLPMYEGRI LGKVERID 

« Hide

References

[1]"Axil, a member of the Axin family, interacts with both glycogen synthase kinase 3beta and beta-catenin and inhibits axis formation of Xenopus embryos."
Yamamoto H., Kishida S., Uochi T., Ikeda S., Koyama S., Asashima M., Kikuchi A.
Mol. Cell. Biol. 18:2867-2875(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CTNNB1 AND GSK3B, TISSUE SPECIFICITY.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF017757 mRNA. Translation: AAC40089.1.
PIRT08423.
RefSeqNP_077331.1. NM_024355.1.
UniGeneRn.162212.

3D structure databases

ProteinModelPortalO70240.
SMRO70240. Positions 67-206, 759-838.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-1534850.
STRING10116.ENSRNOP00000004898.

Proteomic databases

PaxDbO70240.
PRIDEO70240.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID29134.
KEGGrno:29134.

Organism-specific databases

CTD8313.
RGD69259. Axin2.

Phylogenomic databases

eggNOGNOG238205.
HOVERGENHBG004324.
InParanoidO70240.
KOK04385.
OrthoDBEOG79PJQ0.
PhylomeDBO70240.
TreeFamTF315454.

Gene expression databases

GenevestigatorO70240.

Family and domain databases

Gene3D1.10.196.10. 2 hits.
InterProIPR014936. Axin_b-cat-bd.
IPR001158. DIX.
IPR024066. Regulat_G_prot_signal_dom1.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamPF08833. Axin_b-cat_bind. 1 hit.
PF00778. DIX. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSPR01301. RGSPROTEIN.
SMARTSM00021. DAX. 1 hit.
SM00315. RGS. 1 hit.
[Graphical view]
SUPFAMSSF48097. SSF48097. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEPS50841. DIX. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio608087.
PROO70240.

Entry information

Entry nameAXIN2_RAT
AccessionPrimary (citable) accession number: O70240
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: June 11, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families