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Reviewed, UniProtKB/Swiss-Prot O70239 (AXN1_RAT)

Last modified January 19, 2010. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Axin-1
Alternative name(s):
    Axis inhibition protein 1
      Short name=rAxin
Gene names
Name: Axin1
Synonyms: Axin
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length827 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Controls dorsoventral patterning via two opposing effects; down-regulates beta-catenin to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of beta-catenin and APC by GSK3B. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7 By similarity.

Subunit structure

Homodimer. Interacts with GSK3B and beta-catenin. The interaction between axin and beta-catenin occurs via the armadillo repeats contained in beta-catenin. Ternary complex. Also binds to plakoglobin (gamma-catenin), APC, DVL and PP2A. Interacts with TP53 and HIPK2. Probably part of a complex consisting of TP53, HIPK2 and AXIN1. Interacts with SMAD6, SMAD7 and RNF111. Interacts with DIXDC1; prevents interaction with MAP3K1. Interacts with MAP3K4, MDFI and MDFIC. Interacts with ANKRD6. Interacts with AIDA By similarity.

Subcellular location

Cytoplasm By similarity.

Tissue specificity

Highly expressed in testis, thymus and lung followed by cerebrum, cerebellum, heart, kidney, skeletal muscle, spleen and liver.

Post-translational modification

Probably phosphorylated by GSK3B and dephosphorylated by PP2A By similarity.

Sequence similarities

Contains 1 DIX domain.

Contains 1 RGS domain.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 827827Axin-1
PRO_0000220890

Regions

Domain88 – 211124RGS
Domain745 – 82783DIX
Region209 – 338130Interaction with TP53 By similarity
Region348 – 43285Interaction with GSK3B
Region433 – 50169Interaction with beta-catenin

Amino acid modifications

Modified residue4921Phosphoserine By similarity

Secondary structure

1 ............. 827
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
O70239-1 [UniParc].

Last modified May 10, 2002. Version 3.
Checksum: BB1EE9CCECF6D487

FASTA82792,285
        10         20         30         40         50         60 
MNVQEQGFPL DLGASFTEDA PRPPVPGEEG ELVSTDSRPV NHSFCSGKGT SIKSETSTAT 

        70         80         90        100        110        120 
PRRSDLDLGY EPEGSASPTP PYLRWAESLH SLLDDQDGIS LFRTFLKQEG CADLLDFWFA 

       130        140        150        160        170        180 
CSGFRKLEPC DSNEEKRLKL ARAIYRKYIL DSNGIVSRQT KPATKSFIKD CVMKQQIDPA 

       190        200        210        220        230        240 
MFDQAQTEIQ STMEENTYPS FLKSDIYLEY TRTGSESPKV CSDQSSGSGT GKGMSGYLPT 

       250        260        270        280        290        300 
LNEDEEWKCD QDADEDDGRD SVPPSRLTQK LLLETAAPRA PSSRRYNEGR ELRYGSWREP 

       310        320        330        340        350        360 
VNPYYVNSGY ALAPATSAND SEQQSLSSDA DTLSLTDSSV DGIPPYRIRK QHRREMQESV 

       370        380        390        400        410        420 
QVNGRVPLPH IPRTYRMPKE IRVEPQKFAE ELIHRLEAVQ RTREAEEKLE ERLKRVRMEE 

       430        440        450        460        470        480 
EGEDGEMPSG PMASHKLPSV PAWHHFPPRY VDMGCSGLRD AHEENPESIL DEHVQRVMRT 

       490        500        510        520        530        540 
PGCQSPGPGH RSPDSGHVAK TAVLGGTASG HGKHAPKLGL KLDSAGLHHH RHVHHHVHHN 

       550        560        570        580        590        600 
SARPKEQMEA EAARRVQSSF SWGPETHGHA KPRSYSESTG TNPSAGDLAF GGKASAPSKR 

       610        620        630        640        650        660 
NTKKAESGKN ASAEVPSTTE DAEKNQKIMQ WIIEGEKEIS RHRKAGHGSS GMRKQQAHES 

       670        680        690        700        710        720 
SRPLSIERPG AVHPWVSAQL RNSVQPSHLF IQDPTMPPNP APNPLTQLEE ARRRLEEEEK 

       730        740        750        760        770        780 
RANKLPSKQR TKSQRKAGGG SAPPCDSIVV AYYFCGEPIP YRTLVRGRAV TLGQFKELLT 

       790        800        810        820 
KKGSYRYYFK KVSDEFDCGV VFEEVREDEA ILPVFEEKII GKVEKVD

« Hide

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References

[1]"Axin, a negative regulator of the Wnt signaling pathway, forms a complex with GSK-3beta and beta-catenin and promotes GSK-3beta-dependent phosphorylation of beta-catenin."
Ikeda S., Kishida S., Yamamoto H., Murai H., Koyama S., Kikuchi A.
EMBO J. 17:1371-1384(1998) [PubMed: 9482734] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF017756 mRNA. Translation: AAC40066.1. Different initiation.
IPIIPI00231576.
PIRT08422.
RefSeqNP_077381.1.
UniGeneRn.31781

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1WSPX-ray2.90A/B/C744-827[»]
2D5GX-ray3.20A/B/C/D/E/F743-827[»]
SMRO70239. Positions 74-220.
ModBaseSearch...

Protein-protein interaction databases

STRINGO70239.

PTM databases

PhosphoSiteO70239.

Genome annotation databases

EnsemblENSRNOT00000027705; ENSRNOP00000027705; ENSRNOG00000020414; Rattus norvegicus. [Genome view]
GeneID79257.
KEGGrno:79257.
UCSCAF017756. rat.

Organism-specific databases

CTD79257.
RGD620859. Axin1.

Phylogenomic databases

eggNOGroNOG11401.
HOVERGENO70239.

Gene expression databases

ArrayExpressO70239.
GenevestigatorO70239.
GermOnlineENSRNOG00000020414. Rattus norvegicus.

Family and domain databases

InterProIPR014936. Axin_b-cat_bd.
IPR001158. DIX.
IPR000342. Regulat_G_prot_signal.
IPR016137. Regulat_G_prot_signal_superfam.
[Graphical view]
PfamPF08833. Axin_b-cat_bind. 1 hit.
PF00778. DIX. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSPR01301. RGSPROTEIN.
SMARTSM00021. DAX. 1 hit.
SM00315. RGS. 1 hit.
[Graphical view]
PROSITEPS50841. DIX. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio614758.

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Entry information

Entry nameAXN1_RAT
AccessionPrimary (citable) accession number: O70239
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 10, 2002
Last modified: January 19, 2010
This is version 89 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents