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O70230

- ZN143_MOUSE

UniProt

O70230 - ZN143_MOUSE

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Protein

Zinc finger protein 143

Gene

Znf143

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Transcriptional activator. Activates the gene for selenocysteine tRNA (tRNAsec). Binds to the SPH motif of small nuclear RNA (snRNA) gene promoters. Participates in efficient U6 RNA polymerase III transcription via its interaction with CHD8 (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri237 – 26125C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri267 – 29125C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri297 – 32125C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri327 – 35125C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri357 – 38125C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri387 – 41125C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri417 – 44024C2H2-type 7PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 143
Short name:
Zfp-143
Alternative name(s):
Selenocysteine tRNA gene transcription-activating factor
Short name:
mStaf
Gene namesi
Name:Znf143
Synonyms:Staf, Zfp143
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1277969. Zfp143.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 638638Zinc finger protein 143PRO_0000248071Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiO70230.
PaxDbiO70230.
PRIDEiO70230.

PTM databases

PhosphoSiteiO70230.

Expressioni

Gene expression databases

BgeeiO70230.
CleanExiMM_ZFP143.
GenevestigatoriO70230.

Interactioni

Subunit structurei

Interacts with CHD8.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Sall4Q8BX222EBI-5691478,EBI-2312582

Protein-protein interaction databases

BioGridi203516. 3 interactions.
IntActiO70230. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliO70230.
SMRiO70230. Positions 231-438.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 7 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri237 – 26125C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri267 – 29125C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri297 – 32125C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri327 – 35125C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri357 – 38125C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri387 – 41125C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri417 – 44024C2H2-type 7PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00760000118771.
HOGENOMiHOG000118073.
HOVERGENiHBG053078.
InParanoidiO70230.
OMAiKMQIVLQ.
OrthoDBiEOG7WX080.
PhylomeDBiO70230.
TreeFamiTF333498.

Family and domain databases

Gene3Di3.30.160.60. 7 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 7 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 7 hits.
PS50157. ZINC_FINGER_C2H2_2. 7 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O70230-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLAQINRDS QGMTEFPGGG MEAQHVTLCL TEAVTVADGD NLENMEGVSL
60 70 80 90 100
QAVTLADGST AYIQHNSKDG RLIDGQVIQL EDGSAAYVQH VPIPKSTGDS
110 120 130 140 150
LRLEDGQAVQ LEDGTTAFIH HTSKDSYDQS SLQAVQLEDG TTAYIHHAVQ
160 170 180 190 200
VPQSDTILAI QADGTVAGLH TGDATIDPDT ISALEQYAAK VSIDGSDGVT
210 220 230 240 250
STGMIGENEQ EKKMQIVLQG HATRVTPKSQ QSGEKAFRCK YDGCGKLYTT
260 270 280 290 300
AHHLKVHERS HTGDRPYQCE HSGCGKAFAT GYGLKSHFRT HTGEKPYRCS
310 320 330 340 350
EDNCTKSFKT SGDLQKHIRT HTGERPFKCP IEGCGRSFTT SNIRKVHIRT
360 370 380 390 400
HTGERPYYCT EPGCGRAFAS ATNYKNHVRI HTGEKPYVCT VPGCDKRFTE
410 420 430 440 450
YSSLYKHHVV HTHSKPYNCN HCGKTYKQIS TLAMHKRTAH NDTEPIEEEQ
460 470 480 490 500
EAFFEPPPGQ GDDVLKGSQI TYVTGVDGED IVSTQVATVT QSGLSQQVTL
510 520 530 540 550
ISQDGTQHVN ISQADMQAIG NTITMVTQDG TPITVPTHDA VISSAGTHSV
560 570 580 590 600
AMVTAEGTEG QQVAIVAQDL AAFHTASSEM GHQQHSHHLV TTETRPLTLV
610 620 630
ATSNGTQIAV QLGEQPSLEE AIRIASRIQQ GETPGLDD
Length:638
Mass (Da):69,040
Last modified:May 5, 2009 - v2
Checksum:i9BDA0B6C0EBC0634
GO
Isoform 2 (identifier: O70230-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     97-98: TG → R

Show »
Length:637
Mass (Da):69,038
Checksum:iAF55892CDA7BD887
GO
Isoform 3 (identifier: O70230-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     97-125: TGDSLRLEDGQAVQLEDGTTAFIHHTSKD → N

Show »
Length:610
Mass (Da):66,030
Checksum:i3E608E4FA31A3CDE
GO

Sequence cautioni

The sequence BAC25726.1 differs from that shown. Reason: Frameshift at position 5.
The sequence AAC16899.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAH37658.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAC26281.1 differs from that shown. Reason: Erroneous initiation.
The sequence CAC17144.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti190 – 1901K → T in BAC25726. (PubMed:16141072)Curated
Sequence conflicti250 – 2501T → K in BAC25726. (PubMed:16141072)Curated
Sequence conflicti261 – 2611H → D in BAC25726. (PubMed:16141072)Curated
Sequence conflicti342 – 3421N → T in BAC25726. (PubMed:16141072)Curated
Sequence conflicti508 – 5081H → N in BAC25726. (PubMed:16141072)Curated
Sequence conflicti528 – 5281Q → K in BAC25726. (PubMed:16141072)Curated
Sequence conflicti618 – 6181L → V in BAC25726. (PubMed:16141072)Curated
Sequence conflicti634 – 6341P → A in BAC25726. (PubMed:16141072)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei97 – 12529TGDSL…HTSKD → N in isoform 3. 1 PublicationVSP_036979Add
BLAST
Alternative sequencei97 – 982TG → R in isoform 2. 1 PublicationVSP_036980

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF011758 mRNA. Translation: AAC16899.1. Different initiation.
AJ278435 Genomic DNA. Translation: CAC17144.1. Different initiation.
AK028056 mRNA. Translation: BAC25726.1. Frameshift.
AK029078 mRNA. Translation: BAC26281.1. Different initiation.
AK029926 mRNA. Translation: BAC26682.1.
BC037658 mRNA. Translation: AAH37658.1. Different initiation.
CCDSiCCDS57580.1. [O70230-2]
RefSeqiNP_033307.2. NM_009281.3. [O70230-2]
XP_006507595.1. XM_006507532.1. [O70230-1]
UniGeneiMm.10815.

Genome annotation databases

EnsembliENSMUST00000084727; ENSMUSP00000081778; ENSMUSG00000061079. [O70230-2]
ENSMUST00000169638; ENSMUSP00000126015; ENSMUSG00000061079. [O70230-3]
GeneIDi20841.
KEGGimmu:20841.
UCSCiuc009jew.1. mouse. [O70230-2]
uc009jex.1. mouse. [O70230-1]
uc012fse.1. mouse. [O70230-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF011758 mRNA. Translation: AAC16899.1 . Different initiation.
AJ278435 Genomic DNA. Translation: CAC17144.1 . Different initiation.
AK028056 mRNA. Translation: BAC25726.1 . Frameshift.
AK029078 mRNA. Translation: BAC26281.1 . Different initiation.
AK029926 mRNA. Translation: BAC26682.1 .
BC037658 mRNA. Translation: AAH37658.1 . Different initiation.
CCDSi CCDS57580.1. [O70230-2 ]
RefSeqi NP_033307.2. NM_009281.3. [O70230-2 ]
XP_006507595.1. XM_006507532.1. [O70230-1 ]
UniGenei Mm.10815.

3D structure databases

ProteinModelPortali O70230.
SMRi O70230. Positions 231-438.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 203516. 3 interactions.
IntActi O70230. 3 interactions.

PTM databases

PhosphoSitei O70230.

Proteomic databases

MaxQBi O70230.
PaxDbi O70230.
PRIDEi O70230.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000084727 ; ENSMUSP00000081778 ; ENSMUSG00000061079 . [O70230-2 ]
ENSMUST00000169638 ; ENSMUSP00000126015 ; ENSMUSG00000061079 . [O70230-3 ]
GeneIDi 20841.
KEGGi mmu:20841.
UCSCi uc009jew.1. mouse. [O70230-2 ]
uc009jex.1. mouse. [O70230-1 ]
uc012fse.1. mouse. [O70230-3 ]

Organism-specific databases

CTDi 20841.
MGIi MGI:1277969. Zfp143.

Phylogenomic databases

eggNOGi COG5048.
GeneTreei ENSGT00760000118771.
HOGENOMi HOG000118073.
HOVERGENi HBG053078.
InParanoidi O70230.
OMAi KMQIVLQ.
OrthoDBi EOG7WX080.
PhylomeDBi O70230.
TreeFami TF333498.

Miscellaneous databases

NextBioi 299593.
PROi O70230.
SOURCEi Search...

Gene expression databases

Bgeei O70230.
CleanExi MM_ZFP143.
Genevestigatori O70230.

Family and domain databases

Gene3Di 3.30.160.60. 7 hits.
InterProi IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view ]
Pfami PF00096. zf-C2H2. 1 hit.
[Graphical view ]
SMARTi SM00355. ZnF_C2H2. 7 hits.
[Graphical view ]
PROSITEi PS00028. ZINC_FINGER_C2H2_1. 7 hits.
PS50157. ZINC_FINGER_C2H2_2. 7 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of the murine staf cDNA encoding a transcription activating factor for the selenocysteine tRNA gene in mouse mammary gland."
    Adachi K., Saito H., Tanaka T., Oka T.
    J. Biol. Chem. 273:8598-8606(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
    Strain: 129/SvJ.
    Tissue: Liver.
  2. "Genomic organization, chromosomal mapping and promoter analysis of the mouse selenocysteine tRNA gene transcription-activating factor (mStaf) gene."
    Adachi K., Katsuyama M., Song S., Oka T.
    Biochem. J. 346:45-51(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Comparative architectural aspects of regions of conserved synteny on human chromosome 11p15.3 and mouse chromosome 7 (including genes WEE1 and LMO1)."
    Cichutek A., Brueckmann T., Seipel B., Hauser H., Schlaubitz S., Prawitt D., Hankeln T., Schmidt E.R., Winterpacht A., Zabel B.U.
    Cytogenet. Cell Genet. 93:277-283(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Cerebellum, Skin and Testis.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: FVB/N.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiZN143_MOUSE
AccessioniPrimary (citable) accession number: O70230
Secondary accession number(s): Q8BGB0, Q8CEI6, Q8CI27
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: May 5, 2009
Last modified: October 29, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3