O70230 (ZN143_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger protein 143
Short name=Zfp-143

Alternative name(s):
Selenocysteine tRNA gene transcription-activating factor
Short name=mStaf

Gene names

Name:	Znf143
Synonyms:	Staf, Zfp143

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	638 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional activator. Activates the gene for selenocysteine tRNA (tRNAsec). Binds to the SPH motif of small nuclear RNA (snRNA) gene promoters. Participates in efficient U6 RNA polymerase III transcription via its interaction with CHD8 By similarity. Ref.1
Subunit structure	Interacts with CHD8 By similarity.
Subcellular location	Nucleus Potential.
Sequence similarities	Belongs to the GLI C2H2-type zinc-finger protein family. Contains 7 C2H2-type zinc fingers.
Sequence caution	The sequence AAC16899.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH37658.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC25726.1 differs from that shown. Reason: Frameshift at position 5. The sequence BAC26281.1 differs from that shown. Reason: Erroneous initiation. The sequence CAC17144.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from direct assay PubMed 14667815. Source: MGI zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Sall4	Q8BX22	2	EBI-5691478,EBI-2312582

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O70230-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O70230-2)

The sequence of this isoform differs from the canonical sequence as follows:
     97-98: TG → R

Isoform 3 (identifier: O70230-3)

The sequence of this isoform differs from the canonical sequence as follows:
     97-125: TGDSLRLEDGQAVQLEDGTTAFIHHTSKD → N

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 638	638	Zinc finger protein 143		PRO_0000248071
Regions
	Zinc finger	237 – 261	25	C2H2-type 1
	Zinc finger	267 – 291	25	C2H2-type 2
	Zinc finger	297 – 321	25	C2H2-type 3
	Zinc finger	327 – 351	25	C2H2-type 4
	Zinc finger	357 – 381	25	C2H2-type 5
	Zinc finger	387 – 411	25	C2H2-type 6
	Zinc finger	417 – 440	24	C2H2-type 7
Natural variations
	Alternative sequence	97 – 125	29	TGDSL…HTSKD → N in isoform 3.		VSP_036979
	Alternative sequence	97 – 98	2	TG → R in isoform 2.		VSP_036980
Experimental info
	Sequence conflict	190	1	K → T in BAC25726. Ref.4
	Sequence conflict	250	1	T → K in BAC25726. Ref.4
	Sequence conflict	261	1	H → D in BAC25726. Ref.4
	Sequence conflict	342	1	N → T in BAC25726. Ref.4
	Sequence conflict	508	1	H → N in BAC25726. Ref.4
	Sequence conflict	528	1	Q → K in BAC25726. Ref.4
	Sequence conflict	618	1	L → V in BAC25726. Ref.4
	Sequence conflict	634	1	P → A in BAC25726. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 5, 2009. Version 2. Checksum: 9BDA0B6C0EBC0634	FASTA	638	69,040
	10 20 30 40 50 60 MLLAQINRDS QGMTEFPGGG MEAQHVTLCL TEAVTVADGD NLENMEGVSL QAVTLADGST 70 80 90 100 110 120 AYIQHNSKDG RLIDGQVIQL EDGSAAYVQH VPIPKSTGDS LRLEDGQAVQ LEDGTTAFIH 130 140 150 160 170 180 HTSKDSYDQS SLQAVQLEDG TTAYIHHAVQ VPQSDTILAI QADGTVAGLH TGDATIDPDT 190 200 210 220 230 240 ISALEQYAAK VSIDGSDGVT STGMIGENEQ EKKMQIVLQG HATRVTPKSQ QSGEKAFRCK 250 260 270 280 290 300 YDGCGKLYTT AHHLKVHERS HTGDRPYQCE HSGCGKAFAT GYGLKSHFRT HTGEKPYRCS 310 320 330 340 350 360 EDNCTKSFKT SGDLQKHIRT HTGERPFKCP IEGCGRSFTT SNIRKVHIRT HTGERPYYCT 370 380 390 400 410 420 EPGCGRAFAS ATNYKNHVRI HTGEKPYVCT VPGCDKRFTE YSSLYKHHVV HTHSKPYNCN 430 440 450 460 470 480 HCGKTYKQIS TLAMHKRTAH NDTEPIEEEQ EAFFEPPPGQ GDDVLKGSQI TYVTGVDGED 490 500 510 520 530 540 IVSTQVATVT QSGLSQQVTL ISQDGTQHVN ISQADMQAIG NTITMVTQDG TPITVPTHDA 550 560 570 580 590 600 VISSAGTHSV AMVTAEGTEG QQVAIVAQDL AAFHTASSEM GHQQHSHHLV TTETRPLTLV 610 620 630 ATSNGTQIAV QLGEQPSLEE AIRIASRIQQ GETPGLDD
	Isoform 2 [UniParc]. Checksum: AF55892CDA7BD887	FASTA	637	69,038
	10 20 30 40 50 60 MLLAQINRDS QGMTEFPGGG MEAQHVTLCL TEAVTVADGD NLENMEGVSL QAVTLADGST 70 80 90 100 110 120 AYIQHNSKDG RLIDGQVIQL EDGSAAYVQH VPIPKSRDSL RLEDGQAVQL EDGTTAFIHH 130 140 150 160 170 180 TSKDSYDQSS LQAVQLEDGT TAYIHHAVQV PQSDTILAIQ ADGTVAGLHT GDATIDPDTI 190 200 210 220 230 240 SALEQYAAKV SIDGSDGVTS TGMIGENEQE KKMQIVLQGH ATRVTPKSQQ SGEKAFRCKY 250 260 270 280 290 300 DGCGKLYTTA HHLKVHERSH TGDRPYQCEH SGCGKAFATG YGLKSHFRTH TGEKPYRCSE 310 320 330 340 350 360 DNCTKSFKTS GDLQKHIRTH TGERPFKCPI EGCGRSFTTS NIRKVHIRTH TGERPYYCTE 370 380 390 400 410 420 PGCGRAFASA TNYKNHVRIH TGEKPYVCTV PGCDKRFTEY SSLYKHHVVH THSKPYNCNH 430 440 450 460 470 480 CGKTYKQIST LAMHKRTAHN DTEPIEEEQE AFFEPPPGQG DDVLKGSQIT YVTGVDGEDI 490 500 510 520 530 540 VSTQVATVTQ SGLSQQVTLI SQDGTQHVNI SQADMQAIGN TITMVTQDGT PITVPTHDAV 550 560 570 580 590 600 ISSAGTHSVA MVTAEGTEGQ QVAIVAQDLA AFHTASSEMG HQQHSHHLVT TETRPLTLVA 610 620 630 TSNGTQIAVQ LGEQPSLEEA IRIASRIQQG ETPGLDD
	Isoform 3 [UniParc]. Checksum: 3E608E4FA31A3CDE	FASTA	610	66,030
	10 20 30 40 50 60 MLLAQINRDS QGMTEFPGGG MEAQHVTLCL TEAVTVADGD NLENMEGVSL QAVTLADGST 70 80 90 100 110 120 AYIQHNSKDG RLIDGQVIQL EDGSAAYVQH VPIPKSNSYD QSSLQAVQLE DGTTAYIHHA 130 140 150 160 170 180 VQVPQSDTIL AIQADGTVAG LHTGDATIDP DTISALEQYA AKVSIDGSDG VTSTGMIGEN 190 200 210 220 230 240 EQEKKMQIVL QGHATRVTPK SQQSGEKAFR CKYDGCGKLY TTAHHLKVHE RSHTGDRPYQ 250 260 270 280 290 300 CEHSGCGKAF ATGYGLKSHF RTHTGEKPYR CSEDNCTKSF KTSGDLQKHI RTHTGERPFK 310 320 330 340 350 360 CPIEGCGRSF TTSNIRKVHI RTHTGERPYY CTEPGCGRAF ASATNYKNHV RIHTGEKPYV 370 380 390 400 410 420 CTVPGCDKRF TEYSSLYKHH VVHTHSKPYN CNHCGKTYKQ ISTLAMHKRT AHNDTEPIEE 430 440 450 460 470 480 EQEAFFEPPP GQGDDVLKGS QITYVTGVDG EDIVSTQVAT VTQSGLSQQV TLISQDGTQH 490 500 510 520 530 540 VNISQADMQA IGNTITMVTQ DGTPITVPTH DAVISSAGTH SVAMVTAEGT EGQQVAIVAQ 550 560 570 580 590 600 DLAAFHTASS EMGHQQHSHH LVTTETRPLT LVATSNGTQI AVQLGEQPSL EEAIRIASRI 610 QQGETPGLDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of the murine staf cDNA encoding a transcription activating factor for the selenocysteine tRNA gene in mouse mammary gland." Adachi K., Saito H., Tanaka T., Oka T. J. Biol. Chem. 273:8598-8606(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION. Strain: 129/SvJ. Tissue: Liver.
[2]	"Genomic organization, chromosomal mapping and promoter analysis of the mouse selenocysteine tRNA gene transcription-activating factor (mStaf) gene." Adachi K., Katsuyama M., Song S., Oka T. Biochem. J. 346:45-51(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Comparative architectural aspects of regions of conserved synteny on human chromosome 11p15.3 and mouse chromosome 7 (including genes WEE1 and LMO1)." Cichutek A., Brueckmann T., Seipel B., Hauser H., Schlaubitz S., Prawitt D., Hankeln T., Schmidt E.R., Winterpacht A., Zabel B.U. Cytogenet. Cell Genet. 93:277-283(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Cerebellum, Skin and Testis.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Strain: FVB/N. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF011758 mRNA. Translation: AAC16899.1. Different initiation. AJ278435 Genomic DNA. Translation: CAC17144.1. Different initiation. AK028056 mRNA. Translation: BAC25726.1. Frameshift. AK029078 mRNA. Translation: BAC26281.1. Different initiation. AK029926 mRNA. Translation: BAC26682.1. BC037658 mRNA. Translation: AAH37658.1. Different initiation.
IPI	IPI00221558. IPI00929799. IPI00929875.
RefSeq	NP_033307.2. NM_009281.3.
UniGene	Mm.10815.
3D structure databases
ProteinModelPortal	O70230.
SMR	O70230. Positions 231-438.
ModBase	Search...
Protein-protein interaction databases
IntAct	O70230. 3 interactions.
PTM databases
PhosphoSite	O70230.
Proteomic databases
PaxDb	O70230.
PRIDE	O70230.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000084727; ENSMUSP00000081778; ENSMUSG00000061079. ENSMUST00000169638; ENSMUSP00000126015; ENSMUSG00000061079.
GeneID	20841.
KEGG	mmu:20841.
UCSC	uc009jew.1. mouse. uc009jex.1. mouse. uc012fse.1. mouse.
Organism-specific databases
CTD	20841.
MGI	MGI:1277969. Zfp143.
Phylogenomic databases
eggNOG	COG5048.
GeneTree	ENSGT00660000095182.
HOGENOM	HOG000118073.
HOVERGEN	HBG053078.
InParanoid	Q8CEI6.
OMA	QEKKMQI.
OrthoDB	EOG44J2HJ.
Gene expression databases
Bgee	O70230.
CleanEx	MM_ZFP143.
Genevestigator	O70230.
GermOnline	ENSMUSG00000061079. Mus musculus.
Family and domain databases
Gene3D	3.30.160.60. 7 hits.
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Pfam	PF00096. zf-C2H2. 1 hit. [Graphical view]
SMART	SM00355. ZnF_C2H2. 7 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 7 hits. PS50157. ZINC_FINGER_C2H2_2. 7 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	299593.
SOURCE	Search...

Entry information

Entry name

ZN143_MOUSE

Accession

Primary (citable) accession number: O70230
Secondary accession number(s): Q8BGB0, Q8CEI6, Q8CI27

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	May 5, 2009
Last modified:	May 29, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program