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O70230

- ZN143_MOUSE

UniProt

O70230 - ZN143_MOUSE

Protein

Zinc finger protein 143

Gene

Znf143

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 2 (05 May 2009)
      Previous versions | rss
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    Functioni

    Transcriptional activator. Activates the gene for selenocysteine tRNA (tRNAsec). Binds to the SPH motif of small nuclear RNA (snRNA) gene promoters. Participates in efficient U6 RNA polymerase III transcription via its interaction with CHD8 By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri237 – 26125C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri267 – 29125C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri297 – 32125C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri327 – 35125C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri357 – 38125C2H2-type 5PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri387 – 41125C2H2-type 6PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri417 – 44024C2H2-type 7PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: IntAct

    GO - Biological processi

    1. regulation of transcription, DNA-templated Source: UniProtKB-KW
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Zinc finger protein 143
    Short name:
    Zfp-143
    Alternative name(s):
    Selenocysteine tRNA gene transcription-activating factor
    Short name:
    mStaf
    Gene namesi
    Name:Znf143
    Synonyms:Staf, Zfp143
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1277969. Zfp143.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 638638Zinc finger protein 143PRO_0000248071Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiO70230.
    PRIDEiO70230.

    PTM databases

    PhosphoSiteiO70230.

    Expressioni

    Gene expression databases

    BgeeiO70230.
    CleanExiMM_ZFP143.
    GenevestigatoriO70230.

    Interactioni

    Subunit structurei

    Interacts with CHD8.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Sall4Q8BX222EBI-5691478,EBI-2312582

    Protein-protein interaction databases

    BioGridi203516. 3 interactions.
    IntActiO70230. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliO70230.
    SMRiO70230. Positions 231-438.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Contains 7 C2H2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri237 – 26125C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri267 – 29125C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri297 – 32125C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri327 – 35125C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri357 – 38125C2H2-type 5PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri387 – 41125C2H2-type 6PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri417 – 44024C2H2-type 7PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5048.
    GeneTreeiENSGT00740000114893.
    HOGENOMiHOG000118073.
    HOVERGENiHBG053078.
    InParanoidiQ8CEI6.
    OMAiKMQIVLQ.
    OrthoDBiEOG7WX080.
    PhylomeDBiO70230.
    TreeFamiTF333498.

    Family and domain databases

    Gene3Di3.30.160.60. 7 hits.
    InterProiIPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    PfamiPF00096. zf-C2H2. 1 hit.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 7 hits.
    [Graphical view]
    PROSITEiPS00028. ZINC_FINGER_C2H2_1. 7 hits.
    PS50157. ZINC_FINGER_C2H2_2. 7 hits.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O70230-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLLAQINRDS QGMTEFPGGG MEAQHVTLCL TEAVTVADGD NLENMEGVSL    50
    QAVTLADGST AYIQHNSKDG RLIDGQVIQL EDGSAAYVQH VPIPKSTGDS 100
    LRLEDGQAVQ LEDGTTAFIH HTSKDSYDQS SLQAVQLEDG TTAYIHHAVQ 150
    VPQSDTILAI QADGTVAGLH TGDATIDPDT ISALEQYAAK VSIDGSDGVT 200
    STGMIGENEQ EKKMQIVLQG HATRVTPKSQ QSGEKAFRCK YDGCGKLYTT 250
    AHHLKVHERS HTGDRPYQCE HSGCGKAFAT GYGLKSHFRT HTGEKPYRCS 300
    EDNCTKSFKT SGDLQKHIRT HTGERPFKCP IEGCGRSFTT SNIRKVHIRT 350
    HTGERPYYCT EPGCGRAFAS ATNYKNHVRI HTGEKPYVCT VPGCDKRFTE 400
    YSSLYKHHVV HTHSKPYNCN HCGKTYKQIS TLAMHKRTAH NDTEPIEEEQ 450
    EAFFEPPPGQ GDDVLKGSQI TYVTGVDGED IVSTQVATVT QSGLSQQVTL 500
    ISQDGTQHVN ISQADMQAIG NTITMVTQDG TPITVPTHDA VISSAGTHSV 550
    AMVTAEGTEG QQVAIVAQDL AAFHTASSEM GHQQHSHHLV TTETRPLTLV 600
    ATSNGTQIAV QLGEQPSLEE AIRIASRIQQ GETPGLDD 638
    Length:638
    Mass (Da):69,040
    Last modified:May 5, 2009 - v2
    Checksum:i9BDA0B6C0EBC0634
    GO
    Isoform 2 (identifier: O70230-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         97-98: TG → R

    Show »
    Length:637
    Mass (Da):69,038
    Checksum:iAF55892CDA7BD887
    GO
    Isoform 3 (identifier: O70230-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         97-125: TGDSLRLEDGQAVQLEDGTTAFIHHTSKD → N

    Show »
    Length:610
    Mass (Da):66,030
    Checksum:i3E608E4FA31A3CDE
    GO

    Sequence cautioni

    The sequence BAC25726.1 differs from that shown. Reason: Frameshift at position 5.
    The sequence AAC16899.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAH37658.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAC26281.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAC17144.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti190 – 1901K → T in BAC25726. (PubMed:16141072)Curated
    Sequence conflicti250 – 2501T → K in BAC25726. (PubMed:16141072)Curated
    Sequence conflicti261 – 2611H → D in BAC25726. (PubMed:16141072)Curated
    Sequence conflicti342 – 3421N → T in BAC25726. (PubMed:16141072)Curated
    Sequence conflicti508 – 5081H → N in BAC25726. (PubMed:16141072)Curated
    Sequence conflicti528 – 5281Q → K in BAC25726. (PubMed:16141072)Curated
    Sequence conflicti618 – 6181L → V in BAC25726. (PubMed:16141072)Curated
    Sequence conflicti634 – 6341P → A in BAC25726. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei97 – 12529TGDSL…HTSKD → N in isoform 3. 1 PublicationVSP_036979Add
    BLAST
    Alternative sequencei97 – 982TG → R in isoform 2. 1 PublicationVSP_036980

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF011758 mRNA. Translation: AAC16899.1. Different initiation.
    AJ278435 Genomic DNA. Translation: CAC17144.1. Different initiation.
    AK028056 mRNA. Translation: BAC25726.1. Frameshift.
    AK029078 mRNA. Translation: BAC26281.1. Different initiation.
    AK029926 mRNA. Translation: BAC26682.1.
    BC037658 mRNA. Translation: AAH37658.1. Different initiation.
    CCDSiCCDS57580.1. [O70230-2]
    RefSeqiNP_033307.2. NM_009281.3. [O70230-2]
    XP_006507595.1. XM_006507532.1. [O70230-1]
    UniGeneiMm.10815.

    Genome annotation databases

    EnsembliENSMUST00000084727; ENSMUSP00000081778; ENSMUSG00000061079. [O70230-2]
    ENSMUST00000169638; ENSMUSP00000126015; ENSMUSG00000061079. [O70230-3]
    GeneIDi20841.
    KEGGimmu:20841.
    UCSCiuc009jew.1. mouse. [O70230-2]
    uc009jex.1. mouse. [O70230-1]
    uc012fse.1. mouse. [O70230-3]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF011758 mRNA. Translation: AAC16899.1 . Different initiation.
    AJ278435 Genomic DNA. Translation: CAC17144.1 . Different initiation.
    AK028056 mRNA. Translation: BAC25726.1 . Frameshift.
    AK029078 mRNA. Translation: BAC26281.1 . Different initiation.
    AK029926 mRNA. Translation: BAC26682.1 .
    BC037658 mRNA. Translation: AAH37658.1 . Different initiation.
    CCDSi CCDS57580.1. [O70230-2 ]
    RefSeqi NP_033307.2. NM_009281.3. [O70230-2 ]
    XP_006507595.1. XM_006507532.1. [O70230-1 ]
    UniGenei Mm.10815.

    3D structure databases

    ProteinModelPortali O70230.
    SMRi O70230. Positions 231-438.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203516. 3 interactions.
    IntActi O70230. 3 interactions.

    PTM databases

    PhosphoSitei O70230.

    Proteomic databases

    PaxDbi O70230.
    PRIDEi O70230.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000084727 ; ENSMUSP00000081778 ; ENSMUSG00000061079 . [O70230-2 ]
    ENSMUST00000169638 ; ENSMUSP00000126015 ; ENSMUSG00000061079 . [O70230-3 ]
    GeneIDi 20841.
    KEGGi mmu:20841.
    UCSCi uc009jew.1. mouse. [O70230-2 ]
    uc009jex.1. mouse. [O70230-1 ]
    uc012fse.1. mouse. [O70230-3 ]

    Organism-specific databases

    CTDi 20841.
    MGIi MGI:1277969. Zfp143.

    Phylogenomic databases

    eggNOGi COG5048.
    GeneTreei ENSGT00740000114893.
    HOGENOMi HOG000118073.
    HOVERGENi HBG053078.
    InParanoidi Q8CEI6.
    OMAi KMQIVLQ.
    OrthoDBi EOG7WX080.
    PhylomeDBi O70230.
    TreeFami TF333498.

    Miscellaneous databases

    NextBioi 299593.
    PROi O70230.
    SOURCEi Search...

    Gene expression databases

    Bgeei O70230.
    CleanExi MM_ZFP143.
    Genevestigatori O70230.

    Family and domain databases

    Gene3Di 3.30.160.60. 7 hits.
    InterProi IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    Pfami PF00096. zf-C2H2. 1 hit.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 7 hits.
    [Graphical view ]
    PROSITEi PS00028. ZINC_FINGER_C2H2_1. 7 hits.
    PS50157. ZINC_FINGER_C2H2_2. 7 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of the murine staf cDNA encoding a transcription activating factor for the selenocysteine tRNA gene in mouse mammary gland."
      Adachi K., Saito H., Tanaka T., Oka T.
      J. Biol. Chem. 273:8598-8606(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
      Strain: 129/SvJ.
      Tissue: Liver.
    2. "Genomic organization, chromosomal mapping and promoter analysis of the mouse selenocysteine tRNA gene transcription-activating factor (mStaf) gene."
      Adachi K., Katsuyama M., Song S., Oka T.
      Biochem. J. 346:45-51(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Comparative architectural aspects of regions of conserved synteny on human chromosome 11p15.3 and mouse chromosome 7 (including genes WEE1 and LMO1)."
      Cichutek A., Brueckmann T., Seipel B., Hauser H., Schlaubitz S., Prawitt D., Hankeln T., Schmidt E.R., Winterpacht A., Zabel B.U.
      Cytogenet. Cell Genet. 93:277-283(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J.
      Tissue: Cerebellum, Skin and Testis.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
      Strain: FVB/N.
      Tissue: Mammary tumor.

    Entry informationi

    Entry nameiZN143_MOUSE
    AccessioniPrimary (citable) accession number: O70230
    Secondary accession number(s): Q8BGB0, Q8CEI6, Q8CI27
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: May 5, 2009
    Last modified: October 1, 2014
    This is version 101 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3