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Protein

Zinc finger protein 143

Gene

Znf143

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator. Activates the gene for selenocysteine tRNA (tRNAsec). Binds to the SPH motif of small nuclear RNA (snRNA) gene promoters. Participates in efficient U6 RNA polymerase III transcription via its interaction with CHD8 (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri237 – 261C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri267 – 291C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri297 – 321C2H2-type 3PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri327 – 351C2H2-type 4PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri357 – 381C2H2-type 5PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri387 – 411C2H2-type 6PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri417 – 440C2H2-type 7PROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

  • DNA binding Source: MGI
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-6807505. RNA polymerase II transcribes snRNA genes.
R-MMU-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 143
Short name:
Zfp-143
Alternative name(s):
Selenocysteine tRNA gene transcription-activating factor
Short name:
mStaf
Gene namesi
Name:Znf143
Synonyms:Staf, Zfp143
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1277969. Zfp143.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002480711 – 638Zinc finger protein 143Add BLAST638

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei352PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO70230.
MaxQBiO70230.
PaxDbiO70230.
PeptideAtlasiO70230.
PRIDEiO70230.

PTM databases

iPTMnetiO70230.
PhosphoSitePlusiO70230.

Expressioni

Gene expression databases

BgeeiENSMUSG00000061079.
CleanExiMM_ZFP143.
GenevisibleiO70230. MM.

Interactioni

Subunit structurei

Interacts with CHD8.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Sall4Q8BX222EBI-5691478,EBI-2312582

Protein-protein interaction databases

BioGridi203516. 4 interactors.
IntActiO70230. 3 interactors.
STRINGi10090.ENSMUSP00000081778.

Structurei

3D structure databases

ProteinModelPortaliO70230.
SMRiO70230.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 7 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri237 – 261C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri267 – 291C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri297 – 321C2H2-type 3PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri327 – 351C2H2-type 4PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri357 – 381C2H2-type 5PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri387 – 411C2H2-type 6PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri417 – 440C2H2-type 7PROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00760000118771.
HOGENOMiHOG000118073.
HOVERGENiHBG053078.
InParanoidiO70230.
KOiK20828.
OMAiKMQIVLQ.
OrthoDBiEOG091G0N38.
PhylomeDBiO70230.
TreeFamiTF333498.

Family and domain databases

Gene3Di3.30.160.60. 7 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 7 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 7 hits.
PS50157. ZINC_FINGER_C2H2_2. 7 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O70230-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLAQINRDS QGMTEFPGGG MEAQHVTLCL TEAVTVADGD NLENMEGVSL
60 70 80 90 100
QAVTLADGST AYIQHNSKDG RLIDGQVIQL EDGSAAYVQH VPIPKSTGDS
110 120 130 140 150
LRLEDGQAVQ LEDGTTAFIH HTSKDSYDQS SLQAVQLEDG TTAYIHHAVQ
160 170 180 190 200
VPQSDTILAI QADGTVAGLH TGDATIDPDT ISALEQYAAK VSIDGSDGVT
210 220 230 240 250
STGMIGENEQ EKKMQIVLQG HATRVTPKSQ QSGEKAFRCK YDGCGKLYTT
260 270 280 290 300
AHHLKVHERS HTGDRPYQCE HSGCGKAFAT GYGLKSHFRT HTGEKPYRCS
310 320 330 340 350
EDNCTKSFKT SGDLQKHIRT HTGERPFKCP IEGCGRSFTT SNIRKVHIRT
360 370 380 390 400
HTGERPYYCT EPGCGRAFAS ATNYKNHVRI HTGEKPYVCT VPGCDKRFTE
410 420 430 440 450
YSSLYKHHVV HTHSKPYNCN HCGKTYKQIS TLAMHKRTAH NDTEPIEEEQ
460 470 480 490 500
EAFFEPPPGQ GDDVLKGSQI TYVTGVDGED IVSTQVATVT QSGLSQQVTL
510 520 530 540 550
ISQDGTQHVN ISQADMQAIG NTITMVTQDG TPITVPTHDA VISSAGTHSV
560 570 580 590 600
AMVTAEGTEG QQVAIVAQDL AAFHTASSEM GHQQHSHHLV TTETRPLTLV
610 620 630
ATSNGTQIAV QLGEQPSLEE AIRIASRIQQ GETPGLDD
Length:638
Mass (Da):69,040
Last modified:May 5, 2009 - v2
Checksum:i9BDA0B6C0EBC0634
GO
Isoform 2 (identifier: O70230-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     97-98: TG → R

Show »
Length:637
Mass (Da):69,038
Checksum:iAF55892CDA7BD887
GO
Isoform 3 (identifier: O70230-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     97-125: TGDSLRLEDGQAVQLEDGTTAFIHHTSKD → N

Show »
Length:610
Mass (Da):66,030
Checksum:i3E608E4FA31A3CDE
GO

Sequence cautioni

The sequence AAC16899 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH37658 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC25726 differs from that shown. Reason: Frameshift at position 5.Curated
The sequence BAC26281 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAC17144 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti190K → T in BAC25726 (PubMed:16141072).Curated1
Sequence conflicti250T → K in BAC25726 (PubMed:16141072).Curated1
Sequence conflicti261H → D in BAC25726 (PubMed:16141072).Curated1
Sequence conflicti342N → T in BAC25726 (PubMed:16141072).Curated1
Sequence conflicti508H → N in BAC25726 (PubMed:16141072).Curated1
Sequence conflicti528Q → K in BAC25726 (PubMed:16141072).Curated1
Sequence conflicti618L → V in BAC25726 (PubMed:16141072).Curated1
Sequence conflicti634P → A in BAC25726 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03697997 – 125TGDSL…HTSKD → N in isoform 3. 1 PublicationAdd BLAST29
Alternative sequenceiVSP_03698097 – 98TG → R in isoform 2. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF011758 mRNA. Translation: AAC16899.1. Different initiation.
AJ278435 Genomic DNA. Translation: CAC17144.1. Different initiation.
AK028056 mRNA. Translation: BAC25726.1. Frameshift.
AK029078 mRNA. Translation: BAC26281.1. Different initiation.
AK029926 mRNA. Translation: BAC26682.1.
BC037658 mRNA. Translation: AAH37658.1. Different initiation.
CCDSiCCDS57580.1. [O70230-2]
RefSeqiNP_033307.2. NM_009281.3. [O70230-2]
XP_006507595.1. XM_006507532.2. [O70230-1]
UniGeneiMm.10815.

Genome annotation databases

EnsembliENSMUST00000084727; ENSMUSP00000081778; ENSMUSG00000061079. [O70230-1]
ENSMUST00000169638; ENSMUSP00000126015; ENSMUSG00000061079. [O70230-3]
ENSMUST00000209505; ENSMUSP00000147673; ENSMUSG00000061079. [O70230-2]
ENSMUST00000211798; ENSMUSP00000148235; ENSMUSG00000061079. [O70230-2]
GeneIDi20841.
KEGGimmu:20841.
UCSCiuc009jew.1. mouse. [O70230-2]
uc009jex.1. mouse. [O70230-1]
uc012fse.1. mouse. [O70230-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF011758 mRNA. Translation: AAC16899.1. Different initiation.
AJ278435 Genomic DNA. Translation: CAC17144.1. Different initiation.
AK028056 mRNA. Translation: BAC25726.1. Frameshift.
AK029078 mRNA. Translation: BAC26281.1. Different initiation.
AK029926 mRNA. Translation: BAC26682.1.
BC037658 mRNA. Translation: AAH37658.1. Different initiation.
CCDSiCCDS57580.1. [O70230-2]
RefSeqiNP_033307.2. NM_009281.3. [O70230-2]
XP_006507595.1. XM_006507532.2. [O70230-1]
UniGeneiMm.10815.

3D structure databases

ProteinModelPortaliO70230.
SMRiO70230.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203516. 4 interactors.
IntActiO70230. 3 interactors.
STRINGi10090.ENSMUSP00000081778.

PTM databases

iPTMnetiO70230.
PhosphoSitePlusiO70230.

Proteomic databases

EPDiO70230.
MaxQBiO70230.
PaxDbiO70230.
PeptideAtlasiO70230.
PRIDEiO70230.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000084727; ENSMUSP00000081778; ENSMUSG00000061079. [O70230-1]
ENSMUST00000169638; ENSMUSP00000126015; ENSMUSG00000061079. [O70230-3]
ENSMUST00000209505; ENSMUSP00000147673; ENSMUSG00000061079. [O70230-2]
ENSMUST00000211798; ENSMUSP00000148235; ENSMUSG00000061079. [O70230-2]
GeneIDi20841.
KEGGimmu:20841.
UCSCiuc009jew.1. mouse. [O70230-2]
uc009jex.1. mouse. [O70230-1]
uc012fse.1. mouse. [O70230-3]

Organism-specific databases

CTDi20841.
MGIiMGI:1277969. Zfp143.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00760000118771.
HOGENOMiHOG000118073.
HOVERGENiHBG053078.
InParanoidiO70230.
KOiK20828.
OMAiKMQIVLQ.
OrthoDBiEOG091G0N38.
PhylomeDBiO70230.
TreeFamiTF333498.

Enzyme and pathway databases

ReactomeiR-MMU-6807505. RNA polymerase II transcribes snRNA genes.
R-MMU-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Miscellaneous databases

PROiO70230.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000061079.
CleanExiMM_ZFP143.
GenevisibleiO70230. MM.

Family and domain databases

Gene3Di3.30.160.60. 7 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 7 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 7 hits.
PS50157. ZINC_FINGER_C2H2_2. 7 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZN143_MOUSE
AccessioniPrimary (citable) accession number: O70230
Secondary accession number(s): Q8BGB0, Q8CEI6, Q8CI27
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: May 5, 2009
Last modified: November 30, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.