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O70230 (ZN143_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger protein 143

Short name=Zfp-143
Alternative name(s):
Selenocysteine tRNA gene transcription-activating factor
Short name=mStaf
Gene names
Name:Znf143
Synonyms:Staf, Zfp143
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length638 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional activator. Activates the gene for selenocysteine tRNA (tRNAsec). Binds to the SPH motif of small nuclear RNA (snRNA) gene promoters. Participates in efficient U6 RNA polymerase III transcription via its interaction with CHD8 By similarity. Ref.1

Subunit structure

Interacts with CHD8 By similarity.

Subcellular location

Nucleus Potential.

Sequence similarities

Belongs to the GLI C2H2-type zinc-finger protein family.

Contains 7 C2H2-type zinc fingers.

Sequence caution

The sequence AAC16899.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAH37658.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAC25726.1 differs from that shown. Reason: Frameshift at position 5.

The sequence BAC26281.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAC17144.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionActivator
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processregulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from direct assay PubMed 14667815. Source: MGI

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction PubMed 20362541. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sall4Q8BX222EBI-5691478,EBI-2312582

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O70230-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O70230-2)

The sequence of this isoform differs from the canonical sequence as follows:
     97-98: TG → R
Isoform 3 (identifier: O70230-3)

The sequence of this isoform differs from the canonical sequence as follows:
     97-125: TGDSLRLEDGQAVQLEDGTTAFIHHTSKD → N

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 638638Zinc finger protein 143
PRO_0000248071

Regions

Zinc finger237 – 26125C2H2-type 1
Zinc finger267 – 29125C2H2-type 2
Zinc finger297 – 32125C2H2-type 3
Zinc finger327 – 35125C2H2-type 4
Zinc finger357 – 38125C2H2-type 5
Zinc finger387 – 41125C2H2-type 6
Zinc finger417 – 44024C2H2-type 7

Amino acid modifications

Modified residue11N-acetylmethionine By similarity

Natural variations

Alternative sequence97 – 12529TGDSL…HTSKD → N in isoform 3.
VSP_036979
Alternative sequence97 – 982TG → R in isoform 2.
VSP_036980

Experimental info

Sequence conflict1901K → T in BAC25726. Ref.4
Sequence conflict2501T → K in BAC25726. Ref.4
Sequence conflict2611H → D in BAC25726. Ref.4
Sequence conflict3421N → T in BAC25726. Ref.4
Sequence conflict5081H → N in BAC25726. Ref.4
Sequence conflict5281Q → K in BAC25726. Ref.4
Sequence conflict6181L → V in BAC25726. Ref.4
Sequence conflict6341P → A in BAC25726. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 5, 2009. Version 2.
Checksum: 9BDA0B6C0EBC0634

FASTA63869,040
        10         20         30         40         50         60 
MLLAQINRDS QGMTEFPGGG MEAQHVTLCL TEAVTVADGD NLENMEGVSL QAVTLADGST 

        70         80         90        100        110        120 
AYIQHNSKDG RLIDGQVIQL EDGSAAYVQH VPIPKSTGDS LRLEDGQAVQ LEDGTTAFIH 

       130        140        150        160        170        180 
HTSKDSYDQS SLQAVQLEDG TTAYIHHAVQ VPQSDTILAI QADGTVAGLH TGDATIDPDT 

       190        200        210        220        230        240 
ISALEQYAAK VSIDGSDGVT STGMIGENEQ EKKMQIVLQG HATRVTPKSQ QSGEKAFRCK 

       250        260        270        280        290        300 
YDGCGKLYTT AHHLKVHERS HTGDRPYQCE HSGCGKAFAT GYGLKSHFRT HTGEKPYRCS 

       310        320        330        340        350        360 
EDNCTKSFKT SGDLQKHIRT HTGERPFKCP IEGCGRSFTT SNIRKVHIRT HTGERPYYCT 

       370        380        390        400        410        420 
EPGCGRAFAS ATNYKNHVRI HTGEKPYVCT VPGCDKRFTE YSSLYKHHVV HTHSKPYNCN 

       430        440        450        460        470        480 
HCGKTYKQIS TLAMHKRTAH NDTEPIEEEQ EAFFEPPPGQ GDDVLKGSQI TYVTGVDGED 

       490        500        510        520        530        540 
IVSTQVATVT QSGLSQQVTL ISQDGTQHVN ISQADMQAIG NTITMVTQDG TPITVPTHDA 

       550        560        570        580        590        600 
VISSAGTHSV AMVTAEGTEG QQVAIVAQDL AAFHTASSEM GHQQHSHHLV TTETRPLTLV 

       610        620        630 
ATSNGTQIAV QLGEQPSLEE AIRIASRIQQ GETPGLDD 

« Hide

Isoform 2 [UniParc].

Checksum: AF55892CDA7BD887
Show »

FASTA63769,038
Isoform 3 [UniParc].

Checksum: 3E608E4FA31A3CDE
Show »

FASTA61066,030

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of the murine staf cDNA encoding a transcription activating factor for the selenocysteine tRNA gene in mouse mammary gland."
Adachi K., Saito H., Tanaka T., Oka T.
J. Biol. Chem. 273:8598-8606(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
Strain: 129/SvJ.
Tissue: Liver.
[2]"Genomic organization, chromosomal mapping and promoter analysis of the mouse selenocysteine tRNA gene transcription-activating factor (mStaf) gene."
Adachi K., Katsuyama M., Song S., Oka T.
Biochem. J. 346:45-51(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Comparative architectural aspects of regions of conserved synteny on human chromosome 11p15.3 and mouse chromosome 7 (including genes WEE1 and LMO1)."
Cichutek A., Brueckmann T., Seipel B., Hauser H., Schlaubitz S., Prawitt D., Hankeln T., Schmidt E.R., Winterpacht A., Zabel B.U.
Cytogenet. Cell Genet. 93:277-283(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Cerebellum, Skin and Testis.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Strain: FVB/N.
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF011758 mRNA. Translation: AAC16899.1. Different initiation.
AJ278435 Genomic DNA. Translation: CAC17144.1. Different initiation.
AK028056 mRNA. Translation: BAC25726.1. Frameshift.
AK029078 mRNA. Translation: BAC26281.1. Different initiation.
AK029926 mRNA. Translation: BAC26682.1.
BC037658 mRNA. Translation: AAH37658.1. Different initiation.
CCDSCCDS57580.1. [O70230-2]
RefSeqNP_033307.2. NM_009281.3. [O70230-2]
XP_006507595.1. XM_006507532.1. [O70230-1]
UniGeneMm.10815.

3D structure databases

ProteinModelPortalO70230.
SMRO70230. Positions 231-438.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203516. 3 interactions.
IntActO70230. 3 interactions.

PTM databases

PhosphoSiteO70230.

Proteomic databases

PaxDbO70230.
PRIDEO70230.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000084727; ENSMUSP00000081778; ENSMUSG00000061079. [O70230-2]
ENSMUST00000169638; ENSMUSP00000126015; ENSMUSG00000061079. [O70230-3]
GeneID20841.
KEGGmmu:20841.
UCSCuc009jew.1. mouse. [O70230-2]
uc009jex.1. mouse. [O70230-1]
uc012fse.1. mouse. [O70230-3]

Organism-specific databases

CTD20841.
MGIMGI:1277969. Zfp143.

Phylogenomic databases

eggNOGCOG5048.
GeneTreeENSGT00740000114893.
HOGENOMHOG000118073.
HOVERGENHBG053078.
InParanoidQ8CEI6.
OMAKMQIVLQ.
OrthoDBEOG7WX080.
PhylomeDBO70230.
TreeFamTF333498.

Gene expression databases

BgeeO70230.
CleanExMM_ZFP143.
GenevestigatorO70230.

Family and domain databases

Gene3D3.30.160.60. 7 hits.
InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 7 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 7 hits.
PS50157. ZINC_FINGER_C2H2_2. 7 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio299593.
PROO70230.
SOURCESearch...

Entry information

Entry nameZN143_MOUSE
AccessionPrimary (citable) accession number: O70230
Secondary accession number(s): Q8BGB0, Q8CEI6, Q8CI27
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: May 5, 2009
Last modified: July 9, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot