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Protein

Probable phospholipid-transporting ATPase IIA

Gene

Atp9a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + H2O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei391 – 39114-aspartylphosphate intermediateBy similarity
Metal bindingi785 – 7851MagnesiumBy similarity
Metal bindingi789 – 7891MagnesiumBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable phospholipid-transporting ATPase IIA (EC:3.6.3.1)
Alternative name(s):
ATPase class II type 9A
Gene namesi
Name:Atp9a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1330826. Atp9a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 6968CytoplasmicSequence analysisAdd
BLAST
Transmembranei70 – 9122HelicalSequence analysisAdd
BLAST
Topological domaini92 – 965ExtracellularSequence analysis
Transmembranei97 – 11923HelicalSequence analysisAdd
BLAST
Topological domaini120 – 303184CytoplasmicSequence analysisAdd
BLAST
Transmembranei304 – 32522HelicalSequence analysisAdd
BLAST
Topological domaini326 – 3327ExtracellularSequence analysis
Transmembranei333 – 35422HelicalSequence analysisAdd
BLAST
Topological domaini355 – 841487CytoplasmicSequence analysisAdd
BLAST
Transmembranei842 – 86221HelicalSequence analysisAdd
BLAST
Topological domaini863 – 87412ExtracellularSequence analysisAdd
BLAST
Transmembranei875 – 89319HelicalSequence analysisAdd
BLAST
Topological domaini894 – 92330CytoplasmicSequence analysisAdd
BLAST
Transmembranei924 – 94219HelicalSequence analysisAdd
BLAST
Topological domaini943 – 9497ExtracellularSequence analysis
Transmembranei950 – 97223HelicalSequence analysisAdd
BLAST
Topological domaini973 – 9786CytoplasmicSequence analysis
Transmembranei979 – 99921HelicalSequence analysisAdd
BLAST
Topological domaini1000 – 10067ExtracellularSequence analysis
Transmembranei1007 – 103024HelicalSequence analysisAdd
BLAST
Topological domaini1031 – 104717CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 10471046Probable phospholipid-transporting ATPase IIAPRO_0000046376Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO70228.
MaxQBiO70228.
PaxDbiO70228.
PRIDEiO70228.

PTM databases

iPTMnetiO70228.
PhosphoSiteiO70228.
SwissPalmiO70228.

Expressioni

Tissue specificityi

Found in most tissues except spleen. Most abundant in brain. Also detected in fetal tissues.

Gene expression databases

BgeeiO70228.
CleanExiMM_ATP9A.
ExpressionAtlasiO70228. baseline and differential.
GenevisibleiO70228. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029060.

Structurei

3D structure databases

ProteinModelPortaliO70228.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0210. Eukaryota.
ENOG410XPYK. LUCA.
GeneTreeiENSGT00550000074723.
HOGENOMiHOG000201571.
HOVERGENiHBG050617.
InParanoidiO70228.
KOiK01530.
PhylomeDBiO70228.

Family and domain databases

Gene3Di2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR030356. ATP9A.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 1 hit.
PTHR24092:SF49. PTHR24092:SF49. 1 hit.
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 2 hits.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O70228-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDSIPLQPV RHKKRVDSRP RAGCCEWLRC CGGGEPRPRT VWLGHPEKRD
60 70 80 90 100
QRYPRNVINN QKYNFFTFLP GVLFSQFRYF FNFYFLLLAC SQFVPEMRLG
110 120 130 140 150
ALYTYWVPLG FVLAVTIIRE AVEEIRCYVR DKEMNSQVYS RLTSRGTVKV
160 170 180 190 200
KSSNIQVGDL ILVEKNQRVP ADMIFLRTSE KNGSCFLRTD QLDGETDWKL
210 220 230 240 250
RLPVACTQRL PTAADLLQIR SYVYAEEPNI DIHNFLGTFT REDSDPPISE
260 270 280 290 300
SLSIENTLWA GTVIASGTVV GVVLYTGREL RSVMNTSDPR SKIGLFDLEV
310 320 330 340 350
NCLTKILFGA LVVVSLVMVA LQHFAGRWYL QIIRFLLLFS NIIPISLRVN
360 370 380 390 400
LDMGKIVYSW VIRRDSKIPG TVVRSSTIPE QLGRISYLLT DKTGTLTQNE
410 420 430 440 450
MVFKRLHLGT VAYGLDSMDE VQSHIFSIYT QQSQDPPAQK GPTVTTKVRR
460 470 480 490 500
TMSSRVHEAV KAIALCHNVT PVYESNGVTD QAEAEKQFED SCRVYQASSP
510 520 530 540 550
DEVALVQWTE SVGLTLVGRD QSSMQLRTPG DQVLNLTILQ VFPFTYESKR
560 570 580 590 600
MGIIVRDEST GEITFYMKGA DVVMAGIVQY NDWLEEECGN MAREGLRVLV
610 620 630 640 650
VAKKSLTEEQ YQDFEARYVQ AKLSVHDRSL KVATVIESLE MEMELLCLTG
660 670 680 690 700
VEDQLQADVR PTLETLRNAG IKVWMLTGDK LETATCTAKN AHLVTRNQDI
710 720 730 740 750
HVFRLVTNRG EAHLELNAFR RKHDCALVIS GDSLEVCLKY YEYEFMELAC
760 770 780 790 800
QCPAVVCCRC APTQKAQIVR LLQERTGKLT CAVGDGGNDV SMIQESDCGV
810 820 830 840 850
GVEGKEGKQA SLAADFSITQ FKHLGRLLMV HGRNSYKRSA ALSQFVIHRS
860 870 880 890 900
LCISTMQAVF SSVFYFASVP LYQGFLIIGY STIYTMFPVF SLVLDKDVKS
910 920 930 940 950
EVAMLYPELY KDLLKGRPLS YKTFLIWVLI SIYQGSTIMY GALLLFESEF
960 970 980 990 1000
VHIVAISFTS LILTELLMVA LTIQTWHWLM TVAELLSLAC YIASLVFLHE
1010 1020 1030 1040
FIDVYFIATL SFLWKVSVIT LVSCLPLYVL KYLRRRFSPP SYSKLTS
Length:1,047
Mass (Da):118,609
Last modified:May 5, 2009 - v3
Checksum:i5CCFAB1A83A7B966
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti29 – 291R → K in AAC05245 (PubMed:9548971).Curated
Sequence conflicti129 – 1291V → I in AAC05245 (PubMed:9548971).Curated
Sequence conflicti138 – 1381V → I in AAC05245 (PubMed:9548971).Curated
Sequence conflicti227 – 2271E → K in AAF08396 (PubMed:11015572).Curated
Sequence conflicti227 – 2271E → K in AAC05245 (PubMed:9548971).Curated
Sequence conflicti243 – 2431D → N in AAF08396 (PubMed:11015572).Curated
Sequence conflicti243 – 2431D → N in AAC05245 (PubMed:9548971).Curated
Sequence conflicti278 – 2781R → K in AAC05245 (PubMed:9548971).Curated
Sequence conflicti279 – 2791E → K in AAF08396 (PubMed:11015572).Curated
Sequence conflicti279 – 2791E → K in AAC05245 (PubMed:9548971).Curated
Sequence conflicti432 – 4321Q → P in AAC05245 (PubMed:9548971).Curated
Sequence conflicti475 – 4751S → C in AAC05245 (PubMed:9548971).Curated
Sequence conflicti504 – 5107ALVQWTE → TLAGKEG in AAH21814 (PubMed:15489334).Curated
Sequence conflicti533 – 5331V → I in AAC05245 (PubMed:9548971).Curated
Sequence conflicti613 – 6131D → H in AAF08396 (PubMed:11015572).Curated
Sequence conflicti742 – 7421E → D in AAC05245 (PubMed:9548971).Curated
Sequence conflicti784 – 7841G → W in AAF08396 (PubMed:11015572).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF152243 mRNA. Translation: AAF08396.1.
BC021814 mRNA. Translation: AAH21814.1.
BC006949 mRNA. Translation: AAH06949.1.
AF011336 mRNA. Translation: AAC05245.1.
CCDSiCCDS38345.1.
PIRiT42229.
RefSeqiNP_056546.2. NM_015731.3.
UniGeneiMm.330819.

Genome annotation databases

EnsembliENSMUST00000029060; ENSMUSP00000029060; ENSMUSG00000027546.
ENSMUST00000178504; ENSMUSP00000136793; ENSMUSG00000027546.
GeneIDi11981.
KEGGimmu:11981.
UCSCiuc008obc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF152243 mRNA. Translation: AAF08396.1.
BC021814 mRNA. Translation: AAH21814.1.
BC006949 mRNA. Translation: AAH06949.1.
AF011336 mRNA. Translation: AAC05245.1.
CCDSiCCDS38345.1.
PIRiT42229.
RefSeqiNP_056546.2. NM_015731.3.
UniGeneiMm.330819.

3D structure databases

ProteinModelPortaliO70228.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029060.

PTM databases

iPTMnetiO70228.
PhosphoSiteiO70228.
SwissPalmiO70228.

Proteomic databases

EPDiO70228.
MaxQBiO70228.
PaxDbiO70228.
PRIDEiO70228.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029060; ENSMUSP00000029060; ENSMUSG00000027546.
ENSMUST00000178504; ENSMUSP00000136793; ENSMUSG00000027546.
GeneIDi11981.
KEGGimmu:11981.
UCSCiuc008obc.1. mouse.

Organism-specific databases

CTDi10079.
MGIiMGI:1330826. Atp9a.

Phylogenomic databases

eggNOGiKOG0210. Eukaryota.
ENOG410XPYK. LUCA.
GeneTreeiENSGT00550000074723.
HOGENOMiHOG000201571.
HOVERGENiHBG050617.
InParanoidiO70228.
KOiK01530.
PhylomeDBiO70228.

Miscellaneous databases

ChiTaRSiAtp9a. mouse.
NextBioi280125.
PROiO70228.
SOURCEiSearch...

Gene expression databases

BgeeiO70228.
CleanExiMM_ATP9A.
ExpressionAtlasiO70228. baseline and differential.
GenevisibleiO70228. MM.

Family and domain databases

Gene3Di2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR030356. ATP9A.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 1 hit.
PTHR24092:SF49. PTHR24092:SF49. 1 hit.
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 2 hits.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Differential expression of putative transbilayer amphipath transporters."
    Halleck M.S., Lawler J.F. Jr., Blackshaw S., Gao L., Nagarajan P., Hacker C., Pyle S., Newman J.T., Nakanishi Y., Ando H., Weinstock D., Williamson P.L., Schlegel R.A.
    Physiol. Genomics 1:139-150(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "Multiple members of a third subfamily of P-type ATPases identified by genomic sequences and ESTs."
    Halleck M.S., Pradhan D., Blackman C.F., Berkes C., Williamson P.L., Schlegel R.A.
    Genome Res. 8:354-361(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 28-1047.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Heart, Kidney and Lung.

Entry informationi

Entry nameiATP9A_MOUSE
AccessioniPrimary (citable) accession number: O70228
Secondary accession number(s): Q8VDI5, Q922L9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 5, 2009
Last modified: May 11, 2016
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.