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Protein

NKG2-D type II integral membrane protein

Gene

Klrk1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Function as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8+ T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. Participates in NK cell-mediated bone marrow graft rejection. May play a regulatory role in differentiation and survival of NK cells. Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Adaptive immunity, Differentiation, Immunity, Innate immunity

Keywords - Ligandi

Lectin

Enzyme and pathway databases

ReactomeiR-RNO-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
R-RNO-2424491. DAP12 signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
NKG2-D type II integral membrane protein
Alternative name(s):
Killer cell lectin-like receptor subfamily K member 1
NK cell receptor D
NK lectin-like receptor
Short name:
NKLLR
NKG2-D-activating NK receptor
NKR-P2
CD_antigen: CD314
Gene namesi
Name:Klrk1
Synonyms:Nkg2d, Nkrp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi3180. Klrk1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5151CytoplasmicSequence analysisAdd
BLAST
Transmembranei52 – 7423Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini75 – 215141ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 215215NKG2-D type II integral membrane proteinPRO_0000046670Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi95 ↔ 104PROSITE-ProRule annotation
Disulfide bondi98 ↔ 109PROSITE-ProRule annotation
Glycosylationi120 – 1201N-linked (GlcNAc...)Sequence analysis
Disulfide bondi126 ↔ 210PROSITE-ProRule annotation
Glycosylationi130 – 1301N-linked (GlcNAc...)Sequence analysis
Glycosylationi162 – 1621N-linked (GlcNAc...)Sequence analysis
Disulfide bondi188 ↔ 202PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO70215.
PRIDEiO70215.

Expressioni

Tissue specificityi

Expressed by natural killer cells and by resting thoracic duct CD4+ and CD8+ T-cells, but not by thymocytes or other hemopoietic cells. Expressed by DC cells.2 Publications

Gene expression databases

ExpressionAtlasiO70215. differential.
GenevisibleiO70215. RN.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Heterohexamer composed of two subunits of KLRK1 and four subunits of HCST/DAP10. Interacts (via transmembrane domain) with HCST/DAP10 (via transmembrane domain); the interaction is required for KLRK1 NK cell surface and induces NK cell-mediated cytotoxicity. Can form disulfide-bonded heterodimer with CD94 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012886.

Structurei

3D structure databases

ProteinModelPortaliO70215.
SMRiO70215. Positions 93-215.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini105 – 211107C-type lectinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 C-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4297. Eukaryota.
ENOG410XPJ1. LUCA.
GeneTreeiENSGT00730000110282.
HOGENOMiHOG000220925.
HOVERGENiHBG052629.
InParanoidiO70215.
KOiK06728.
OMAiNDWICHR.
OrthoDBiEOG7NW6B5.
PhylomeDBiO70215.
TreeFamiTF336674.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR016187. C-type_lectin_fold.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O70215-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKCHNYDLK PAKWDTSQEH QKQRSALPTS RPGENGIIRR RSSIEELKIS
60 70 80 90 100
PLFVVRVLVA AMTIRFTVIT LTWLAVFITL LCNKEVSVSS REGYCGPCPN
110 120 130 140 150
DWICHRNNCY QFFNENKAWN QSQASCLSQN SSLLKIYSKE EQDFLKLVKS
160 170 180 190 200
YHWMGLVQSP ANGSWQWEDG SSLSPNELTL VKTPSGTCAV YGSSFKAYTE
210
DCSNPNTYIC MKRAV
Length:215
Mass (Da):24,438
Last modified:August 1, 1998 - v1
Checksum:iB49C0364613031AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF009511 mRNA. Translation: AAC40092.1.
RefSeqiNP_598196.1. NM_133512.2.
XP_006237139.1. XM_006237077.2.
UniGeneiRn.14544.

Genome annotation databases

EnsembliENSRNOT00000088151; ENSRNOP00000074391; ENSRNOG00000061739.
GeneIDi24934.
KEGGirno:24934.
UCSCiRGD:3180. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF009511 mRNA. Translation: AAC40092.1.
RefSeqiNP_598196.1. NM_133512.2.
XP_006237139.1. XM_006237077.2.
UniGeneiRn.14544.

3D structure databases

ProteinModelPortaliO70215.
SMRiO70215. Positions 93-215.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012886.

Proteomic databases

PaxDbiO70215.
PRIDEiO70215.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000088151; ENSRNOP00000074391; ENSRNOG00000061739.
GeneIDi24934.
KEGGirno:24934.
UCSCiRGD:3180. rat.

Organism-specific databases

CTDi22914.
RGDi3180. Klrk1.

Phylogenomic databases

eggNOGiKOG4297. Eukaryota.
ENOG410XPJ1. LUCA.
GeneTreeiENSGT00730000110282.
HOGENOMiHOG000220925.
HOVERGENiHBG052629.
InParanoidiO70215.
KOiK06728.
OMAiNDWICHR.
OrthoDBiEOG7NW6B5.
PhylomeDBiO70215.
TreeFamiTF336674.

Enzyme and pathway databases

ReactomeiR-RNO-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
R-RNO-2424491. DAP12 signaling.

Miscellaneous databases

NextBioi604911.
PROiO70215.

Gene expression databases

ExpressionAtlasiO70215. differential.
GenevisibleiO70215. RN.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR016187. C-type_lectin_fold.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular characterization of rat NKR-P2, a lectin-like receptor expressed by NK cells and resting T cells."
    Berg S.F., Dissen E., Westgaard I.H., Fossum S.
    Int. Immunol. 10:379-385(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Fischer 344.
    Tissue: Natural killer cell.
  2. "Involvement of NKR-P2/NKG2D in DC-mediated killing of tumor targets: indicative of a common, innate, target-recognition paradigm?"
    Alli R., Savithri B., Das S., Varalakshmi C., Rangaraj N., Khar A.
    Eur. J. Immunol. 34:1119-1126(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiNKG2D_RAT
AccessioniPrimary (citable) accession number: O70215
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: August 1, 1998
Last modified: February 17, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Is not capable of signal transduction by itself, but operates through the adapter protein HCST.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.