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Protein

NKG2-D type II integral membrane protein

Gene

Klrk1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Function as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8+ T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. Participates in NK cell-mediated bone marrow graft rejection. May play a regulatory role in differentiation and survival of NK cells. Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins.1 Publication

Miscellaneous

Is not capable of signal transduction by itself, but operates through the adapter protein HCST.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionReceptor
Biological processAdaptive immunity, Differentiation, Immunity, Innate immunity
LigandLectin

Enzyme and pathway databases

ReactomeiR-RNO-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
R-RNO-2424491. DAP12 signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
NKG2-D type II integral membrane protein
Alternative name(s):
Killer cell lectin-like receptor subfamily K member 1
NK cell receptor D
NK lectin-like receptor
Short name:
NKLLR
NKG2-D-activating NK receptor
NKR-P2
CD_antigen: CD314
Gene namesi
Name:Klrk1
Synonyms:Nkg2d, Nkrp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi3180. Klrk1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 51CytoplasmicSequence analysisAdd BLAST51
Transmembranei52 – 74Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST23
Topological domaini75 – 215ExtracellularSequence analysisAdd BLAST141

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000466701 – 215NKG2-D type II integral membrane proteinAdd BLAST215

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi95 ↔ 104PROSITE-ProRule annotation
Disulfide bondi98 ↔ 109PROSITE-ProRule annotation
Glycosylationi120N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi126 ↔ 210PROSITE-ProRule annotation
Glycosylationi130N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi162N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi188 ↔ 202PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO70215.

Expressioni

Tissue specificityi

Expressed by natural killer cells and by resting thoracic duct CD4+ and CD8+ T-cells, but not by thymocytes or other hemopoietic cells. Expressed by DC cells.2 Publications

Gene expression databases

BgeeiENSRNOG00000061739.
ExpressionAtlasiO70215. differential.
GenevisibleiO70215. RN.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Heterohexamer composed of two subunits of KLRK1 and four subunits of HCST/DAP10. Interacts (via transmembrane domain) with HCST/DAP10 (via transmembrane domain); the interaction is required for KLRK1 NK cell surface and induces NK cell-mediated cytotoxicity. Can form disulfide-bonded heterodimer with CD94 (By similarity). Interacts with CEACAM1; recruits PTPN6 that dephosphorylates VAV1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012886.

Structurei

3D structure databases

ProteinModelPortaliO70215.
SMRiO70215.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini105 – 211C-type lectinPROSITE-ProRule annotationAdd BLAST107

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4297. Eukaryota.
ENOG410XPJ1. LUCA.
GeneTreeiENSGT00730000110282.
HOGENOMiHOG000220925.
HOVERGENiHBG052629.
InParanoidiO70215.
KOiK06728.
PhylomeDBiO70215.
TreeFamiTF336674.

Family and domain databases

CDDicd03593. CLECT_NK_receptors_like. 1 hit.
Gene3Di3.10.100.10. 1 hit.
InterProiView protein in InterPro
IPR001304. C-type_lectin-like.
IPR016186. C-type_lectin-like/link.
IPR016187. CTDL_fold.
IPR033992. NKR-like_CTLD.
PfamiView protein in Pfam
PF00059. Lectin_C. 1 hit.
SMARTiView protein in SMART
SM00034. CLECT. 1 hit.
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiView protein in PROSITE
PS50041. C_TYPE_LECTIN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

O70215-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKCHNYDLK PAKWDTSQEH QKQRSALPTS RPGENGIIRR RSSIEELKIS
60 70 80 90 100
PLFVVRVLVA AMTIRFTVIT LTWLAVFITL LCNKEVSVSS REGYCGPCPN
110 120 130 140 150
DWICHRNNCY QFFNENKAWN QSQASCLSQN SSLLKIYSKE EQDFLKLVKS
160 170 180 190 200
YHWMGLVQSP ANGSWQWEDG SSLSPNELTL VKTPSGTCAV YGSSFKAYTE
210
DCSNPNTYIC MKRAV
Length:215
Mass (Da):24,438
Last modified:August 1, 1998 - v1
Checksum:iB49C0364613031AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF009511 mRNA. Translation: AAC40092.1.
RefSeqiNP_598196.1. NM_133512.2.
XP_006237139.1. XM_006237077.3.
UniGeneiRn.14544.

Genome annotation databases

EnsembliENSRNOT00000088151; ENSRNOP00000074391; ENSRNOG00000061739.
GeneIDi24934.
KEGGirno:24934.
UCSCiRGD:3180. rat.

Similar proteinsi

Entry informationi

Entry nameiNKG2D_RAT
AccessioniPrimary (citable) accession number: O70215
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: August 1, 1998
Last modified: August 30, 2017
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome