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Protein

5-hydroxytryptamine receptor 3A

Gene

HTR3A

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.1 Publication

GO - Molecular functioni

  • extracellular ligand-gated ion channel activity Source: InterPro
  • GABA-A receptor activity Source: UniProtKB

GO - Biological processi

  • cation transport Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 3A
Short name:
5-HT3-A
Short name:
5-HT3A
Alternative name(s):
5-hydroxytryptamine receptor 3
Short name:
5-HT-3
Short name:
5-HT3R
Serotonin receptor 3A
Serotonin-gated ion channel receptor
Gene namesi
Name:HTR3A
Synonyms:5HT3R, HTR3
OrganismiCavia porcellus (Guinea pig)Imported
Taxonomic identifieri10141 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
Proteomesi
  • UP000005447 Componenti: Unassembled WGS sequence

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 249230ExtracellularSequence analysisAdd
BLAST
Transmembranei250 – 27021Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini271 – 28515CytoplasmicSequence analysisAdd
BLAST
Transmembranei286 – 30621Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini307 – 3126ExtracellularSequence analysis
Transmembranei313 – 33321Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini334 – 467134CytoplasmicSequence analysisAdd
BLAST
Transmembranei468 – 48821Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini489 – 4902ExtracellularSequence analysis

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • integral component of synaptic vesicle membrane Source: UniProtKB
  • postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3666.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Chaini20 – 4904715-hydroxytryptamine receptor 3APRO_0000000407Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi33 – 331N-linked (GlcNAc...)Sequence analysis
Glycosylationi109 – 1091N-linked (GlcNAc...)Sequence analysis
Disulfide bondi162 ↔ 176By similarity
Glycosylationi175 – 1751N-linked (GlcNAc...)Sequence analysis
Glycosylationi191 – 1911N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

PTM databases

iPTMnetiO70212.

Expressioni

Tissue specificityi

Expressed in cortex, intestine and liver. Not expressed in muscle or spleen.1 Publication

Interactioni

Subunit structurei

Forms pentahomomeric complex as well as pentaheteromeric complex with HTR3B or HTR3C or HTR3D or HTR3E; homomeric complex is functional but exhibits low conductance with modified voltage dependence, and decreased agonist and antagonist affinity. Interacts with RIC3 (By similarity).By similarity

Chemistry

BindingDBiO70212.

Structurei

3D structure databases

ProteinModelPortaliO70212.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni426 – 46237HA-stretchAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000241519.
HOVERGENiHBG106638.
InParanoidiO70212.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR008132. 5HT3_rcpt.
IPR008133. 5HT3_rcpt_A.
IPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01709. 5HT3ARECEPTR.
PR01708. 5HT3RECEPTOR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O70212-1) [UniParc]FASTAAdd to basket
Also known as: 5-HT3R-L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVLWLQLALL ALLLPTSLAQ GEVRGKGTAQ AHNSTRPALQ RLSDHLLADY
60 70 80 90 100
RKSVRPVRDW RKPTTVSIDA IVYAILSVDE KNQVLTTYIW YRQFWTDEFL
110 120 130 140 150
QWNPEDFDNI TKLSIPTDSI WVPDILINEF VDVGKSPNIP YVYVRHQGEV
160 170 180 190 200
QNYKPLQVVT ACSLDIYNFP FDVQNCSLTF TSWLHTIQDI NISLWRLPEK
210 220 230 240 250
VKSDKSVFMN QGEWELLGVL TEFLEFSDRE SRGSFAEMKF YVVIRRRPLF
260 270 280 290 300
YAVTLLLPSI FLMIVDIVGF YLPPDSGERV SFKITLLLGY SVFLIIVSDT
310 320 330 340 350
LPATAIGTPL ISVYFVVCMA LLVISLAETI LIVRLVHKQD LQQPVPLWLR
360 370 380 390 400
HLVLERIAGL LCLGEQLTSH RGPATLQATK TDDFSGSTLL PAMGNHCGPL
410 420 430 440 450
GGPQDLEKTS RGRGSPPPPP REASLAMCGL LQELASIRHF LEKREETREV
460 470 480 490
ARDWLRVGSV LDKLLFRVYL LAVLAYSITL VTLWSVWHYA
Length:490
Mass (Da):55,656
Last modified:August 1, 1998 - v1
Checksum:iEDB32F8597494F61
GO
Isoform 2 (identifier: O70212-2) [UniParc]FASTAAdd to basket
Also known as: 5-HT3R-S

The sequence of this isoform differs from the canonical sequence as follows:
     386-391: Missing.

Show »
Length:484
Mass (Da):55,087
Checksum:iF7CF69D582ADBC82
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei386 – 3916Missing in isoform 2. 1 PublicationVSP_000077

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF006461 mRNA. Translation: AAC06136.1.
AF006462 mRNA. Translation: AAC06137.1.
RefSeqiNP_001166178.1. NM_001172707.1. [O70212-1]

Genome annotation databases

GeneIDi100135547.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF006461 mRNA. Translation: AAC06136.1.
AF006462 mRNA. Translation: AAC06137.1.
RefSeqiNP_001166178.1. NM_001172707.1. [O70212-1]

3D structure databases

ProteinModelPortaliO70212.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiO70212.
ChEMBLiCHEMBL3666.

PTM databases

iPTMnetiO70212.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100135547.

Organism-specific databases

CTDi3359.

Phylogenomic databases

HOGENOMiHOG000241519.
HOVERGENiHBG106638.
InParanoidiO70212.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR008132. 5HT3_rcpt.
IPR008133. 5HT3_rcpt_A.
IPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01709. 5HT3ARECEPTR.
PR01708. 5HT3RECEPTOR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei5HT3A_CAVPO
AccessioniPrimary (citable) accession number: O70212
Secondary accession number(s): O70213
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: August 1, 1998
Last modified: July 6, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.