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O70201

- BIRC5_MOUSE

UniProt

O70201 - BIRC5_MOUSE

Protein

Baculoviral IAP repeat-containing protein 5

Gene

Birc5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7 By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi57 – 571Zinc 1
    Metal bindingi60 – 601Zinc 1
    Metal bindingi76 – 761Zinc 2
    Metal bindingi77 – 771Zinc 1
    Metal bindingi80 – 801Zinc 2
    Metal bindingi84 – 841Zinc 1

    GO - Molecular functioni

    1. cofactor binding Source: UniProtKB
    2. cysteine-type endopeptidase inhibitor activity involved in apoptotic process Source: UniProtKB
    3. microtubule binding Source: UniProtKB
    4. protein binding Source: MGI
    5. protein homodimerization activity Source: UniProtKB
    6. tubulin binding Source: UniProtKB
    7. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. cell division Source: UniProtKB
    2. chromosome segregation Source: UniProtKB-KW
    3. cytokinesis Source: UniProtKB
    4. establishment of chromosome localization Source: UniProtKB
    5. G2/M transition of mitotic cell cycle Source: UniProtKB
    6. microtubule cytoskeleton organization Source: MGI
    7. mitotic nuclear division Source: UniProtKB-KW
    8. negative regulation of apoptotic process Source: UniProtKB
    9. negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
    10. negative regulation of neuron apoptotic process Source: MGI
    11. negative regulation of transcription, DNA-templated Source: UniProtKB
    12. positive regulation of exit from mitosis Source: UniProtKB
    13. positive regulation of mitotic cell cycle Source: UniProtKB
    14. protein complex localization Source: UniProtKB
    15. protein phosphorylation Source: UniProtKB
    16. regulation of insulin secretion involved in cellular response to glucose stimulus Source: MGI
    17. regulation of mitotic cell cycle Source: MGI
    18. regulation of type B pancreatic cell proliferation Source: MGI
    19. spindle checkpoint Source: UniProtKB
    20. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Protease inhibitor, Repressor, Thiol protease inhibitor

    Keywords - Biological processi

    Apoptosis, Cell cycle, Cell division, Chromosome partition, Mitosis, Transcription, Transcription regulation

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_198961. Resolution of Sister Chromatid Cohesion.
    REACT_207679. Separation of Sister Chromatids.

    Protein family/group databases

    MEROPSiI32.005.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Baculoviral IAP repeat-containing protein 5
    Alternative name(s):
    Apoptosis inhibitor 4
    Apoptosis inhibitor survivin
    TIAP
    Gene namesi
    Name:Birc5
    Synonyms:Api4, Iap4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1203517. Birc5.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity. Chromosome. Chromosomecentromere By similarity. Cytoplasmcytoskeletonspindle By similarity. Chromosomecentromerekinetochore By similarity. Midbody By similarity
    Note: Localizes on chromosome arms and inner centromeres from prophase through metaphase. Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, localizes exclusively to the midbody. Colocalizes with AURKB at mitotic chromosomes. Acetylation at Lys-129 directs its localization to the nucleus by enhancing homodimerization and thereby inhibiting XPO1/CRM1-mediated nuclear export By similarity.By similarity

    GO - Cellular componenti

    1. centriole Source: UniProtKB
    2. chromosome, centromeric region Source: UniProtKB
    3. chromosome passenger complex Source: UniProtKB
    4. condensed chromosome kinetochore Source: UniProtKB
    5. cytoplasm Source: UniProtKB
    6. cytoplasmic microtubule Source: UniProtKB
    7. cytosol Source: UniProtKB
    8. interphase microtubule organizing center Source: UniProtKB
    9. midbody Source: UniProtKB
    10. nucleus Source: UniProtKB
    11. spindle microtubule Source: UniProtKB

    Keywords - Cellular componenti

    Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 140140Baculoviral IAP repeat-containing protein 5PRO_0000122357Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei23 – 231N6-acetyllysineBy similarity
    Modified residuei34 – 341Phosphothreonine; by CDK11 Publication
    Modified residuei48 – 481PhosphothreonineBy similarity
    Modified residuei90 – 901N6-acetyllysineBy similarity
    Modified residuei110 – 1101N6-acetyllysineBy similarity
    Modified residuei112 – 1121N6-acetyllysineBy similarity
    Modified residuei115 – 1151N6-acetyllysineBy similarity
    Modified residuei117 – 1171Phosphothreonine; by AURKBBy similarity
    Modified residuei129 – 1291N6-acetyllysineBy similarity

    Post-translational modificationi

    Ubiquitinated by the Cul9-RING ubiquitin-protein ligase complex, leading to its degradation. Ubiquitination is required for centrosomal targeting By similarity.By similarity
    In vitro phosphorylation at Thr-117 by AURKB prevents interaction with INCENP and localization to mitotic chromosomes.By similarity
    Acetylation at Lys-129 results in its homodimerization, while deacetylation promotes the formation of monomers which heterodimerize with XPO1/CRM1 which facilitates its nuclear export. The acetylated form represses STAT3 transactivation. The dynamic equilibrium between its acetylation and deacetylation at Lys-129 determines its interaction with XPO1/CRM1, its subsequent subcellular localization, and its ability to inhibit STAT3 transactivation By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiO70201.
    PRIDEiO70201.

    PTM databases

    PhosphoSiteiO70201.

    Expressioni

    Gene expression databases

    ArrayExpressiO70201.
    BgeeiO70201.
    CleanExiMM_BIRC5.
    GenevestigatoriO70201.

    Interactioni

    Subunit structurei

    Monomer or homodimer. Exists as a homodimer in the apo state and as a monomer in the CPC-bound state. The monomer protects cells against apoptosis more efficiently than the dimer. Only the dimeric form is capable of enhancing tubulin stability in cells. When phosphorylated, interacts with LAMTOR5/HBXIP; the resulting complex binds pro-CASP9, as well as active CASP9, but much less efficiently. Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB and AURKC. Interacts with JTB. Interacts with CDCA8 and INCENP; interaction is direct. Interacts with EVI5. Interacts with GTP-bound RAN in both the S and M phases of the cell cycle. Interacts with USP9X. Interacts with tubulin. Interacts with BIRC2/c-IAP1. The acetylated form at Lys-129 interacts with STAT3. The monomeric form deacetylated at Lys-129 interacts with XPO1/CRM1. The monomeric form interacts with XIAP/BIRC4. Both the dimeric and monomeric form can interact with DIABLO/SMAC. Interacts with BIRC6/bruce By similarity.By similarity

    Structurei

    Secondary structure

    1
    140
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi11 – 133
    Helixi15 – 206
    Beta strandi29 – 313
    Helixi35 – 406
    Beta strandi43 – 453
    Beta strandi55 – 573
    Turni58 – 603
    Helixi73 – 797
    Helixi85 – 884
    Helixi93 – 953
    Helixi98 – 11619

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1M4MX-ray2.80A1-140[»]
    ProteinModelPortaliO70201.
    SMRiO70201. Positions 7-118.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO70201.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati18 – 8871BIRAdd
    BLAST

    Domaini

    The BIR repeat is necessary and sufficient for LAMTOR5 binding.By similarity

    Sequence similaritiesi

    Belongs to the IAP family.Curated
    Contains 1 BIR repeat.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG271140.
    GeneTreeiENSGT00510000047537.
    HOGENOMiHOG000008032.
    HOVERGENiHBG050690.
    InParanoidiO70201.
    KOiK08731.
    OMAiNSPDIAM.
    OrthoDBiEOG7SFJ01.
    PhylomeDBiO70201.
    TreeFamiTF342652.

    Family and domain databases

    Gene3Di1.10.1170.10. 1 hit.
    InterProiIPR001370. BIR.
    [Graphical view]
    PfamiPF00653. BIR. 1 hit.
    [Graphical view]
    SMARTiSM00238. BIR. 1 hit.
    [Graphical view]
    PROSITEiPS50143. BIR_REPEAT_2. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O70201-1) [UniParc]FASTAAdd to Basket

    Also known as: Survivin 140

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGAPALPQIW QLYLKNYRIA TFKNWPFLED CACTPERMAE AGFIHCPTEN    50
    EPDLAQCFFC FKELEGWEPD DNPIEEHRKH SPGCAFLTVK KQMEELTVSE 100
    FLKLDRQRAK NKIAKETNNK QKEFEETAKT TRQSIEQLAA 140
    Length:140
    Mass (Da):16,298
    Last modified:August 1, 1998 - v1
    Checksum:i26F5ABF501A6D83C
    GO
    Isoform 2 (identifier: O70201-2) [UniParc]FASTAAdd to Basket

    Also known as: Survivin 121

    The sequence of this isoform differs from the canonical sequence as follows:
         114-140: AKETNNKQKEFEETAKTTRQSIEQLAA → VCMIENKD

    Show »
    Length:121
    Mass (Da):14,154
    Checksum:i1E0EC7E01BA65585
    GO
    Isoform 3 (identifier: O70201-3) [UniParc]FASTAAdd to Basket

    Also known as: Survivin 40

    The sequence of this isoform differs from the canonical sequence as follows:
         38-40: MAE → RGA
         41-140: Missing.

    Show »
    Length:40
    Mass (Da):4,670
    Checksum:iFAD3C7E34A20518C
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei38 – 403MAE → RGA in isoform 3. CuratedVSP_002455
    Alternative sequencei41 – 140100Missing in isoform 3. CuratedVSP_002456Add
    BLAST
    Alternative sequencei114 – 14027AKETN…EQLAA → VCMIENKD in isoform 2. 1 PublicationVSP_002457Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF077349 mRNA. Translation: AAD34225.1.
    AB013819 mRNA. Translation: BAA28266.1.
    AF115517 Genomic DNA. Translation: AAD26199.1.
    AF115517 Genomic DNA. Translation: AAD26200.1.
    AF115517 Genomic DNA. Translation: AAD26201.1.
    BC004702 mRNA. Translation: AAH04702.1.
    CCDSiCCDS25694.1. [O70201-1]
    CCDS25695.1. [O70201-2]
    RefSeqiNP_001012273.1. NM_001012273.1. [O70201-2]
    NP_033819.1. NM_009689.2. [O70201-1]
    UniGeneiMm.8552.

    Genome annotation databases

    EnsembliENSMUST00000081387; ENSMUSP00000079124; ENSMUSG00000017716. [O70201-1]
    ENSMUST00000093906; ENSMUSP00000091433; ENSMUSG00000017716. [O70201-2]
    GeneIDi11799.
    KEGGimmu:11799.
    UCSCiuc007mod.1. mouse. [O70201-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF077349 mRNA. Translation: AAD34225.1 .
    AB013819 mRNA. Translation: BAA28266.1 .
    AF115517 Genomic DNA. Translation: AAD26199.1 .
    AF115517 Genomic DNA. Translation: AAD26200.1 .
    AF115517 Genomic DNA. Translation: AAD26201.1 .
    BC004702 mRNA. Translation: AAH04702.1 .
    CCDSi CCDS25694.1. [O70201-1 ]
    CCDS25695.1. [O70201-2 ]
    RefSeqi NP_001012273.1. NM_001012273.1. [O70201-2 ]
    NP_033819.1. NM_009689.2. [O70201-1 ]
    UniGenei Mm.8552.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1M4M X-ray 2.80 A 1-140 [» ]
    ProteinModelPortali O70201.
    SMRi O70201. Positions 7-118.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi I32.005.

    PTM databases

    PhosphoSitei O70201.

    Proteomic databases

    MaxQBi O70201.
    PRIDEi O70201.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000081387 ; ENSMUSP00000079124 ; ENSMUSG00000017716 . [O70201-1 ]
    ENSMUST00000093906 ; ENSMUSP00000091433 ; ENSMUSG00000017716 . [O70201-2 ]
    GeneIDi 11799.
    KEGGi mmu:11799.
    UCSCi uc007mod.1. mouse. [O70201-1 ]

    Organism-specific databases

    CTDi 332.
    MGIi MGI:1203517. Birc5.

    Phylogenomic databases

    eggNOGi NOG271140.
    GeneTreei ENSGT00510000047537.
    HOGENOMi HOG000008032.
    HOVERGENi HBG050690.
    InParanoidi O70201.
    KOi K08731.
    OMAi NSPDIAM.
    OrthoDBi EOG7SFJ01.
    PhylomeDBi O70201.
    TreeFami TF342652.

    Enzyme and pathway databases

    Reactomei REACT_198961. Resolution of Sister Chromatid Cohesion.
    REACT_207679. Separation of Sister Chromatids.

    Miscellaneous databases

    EvolutionaryTracei O70201.
    NextBioi 279641.
    PROi O70201.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O70201.
    Bgeei O70201.
    CleanExi MM_BIRC5.
    Genevestigatori O70201.

    Family and domain databases

    Gene3Di 1.10.1170.10. 1 hit.
    InterProi IPR001370. BIR.
    [Graphical view ]
    Pfami PF00653. BIR. 1 hit.
    [Graphical view ]
    SMARTi SM00238. BIR. 1 hit.
    [Graphical view ]
    PROSITEi PS50143. BIR_REPEAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mammalian inhibitor of apoptosis (IAP) homolog."
      Uren A.G., Vaux D.L.
      Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. Kobayashi K., Otaki M., Ogasawara T., Tokuhisa T.
      Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Embryo.
    3. "Three differentially expressed survivin cDNA variants encode proteins with distinct antiapoptotic functions."
      Conway E.M., Pollefeyt S., Cornelissen J., DeBaere I., Steiner-Mosonyi M., Ong K., Baens M., Collen D., Schuh A.C.
      Blood 95:1435-1442(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1; 2 AND 3).
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    5. "Crystal structure and mutagenic analysis of the inhibitor-of-apoptosis protein survivin."
      Muchmore S.W., Chen J., Jakob C., Zakula D., Matayoshi E.D., Wu W., Zhang H., Li F., Ng S.C., Altieri D.C.
      Mol. Cell 6:173-182(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 7-118, PHOSPHORYLATION AT THR-34.

    Entry informationi

    Entry nameiBIRC5_MOUSE
    AccessioniPrimary (citable) accession number: O70201
    Secondary accession number(s): Q923F7, Q9WU53, Q9WU54
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 139 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3