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O70201

- BIRC5_MOUSE

UniProt

O70201 - BIRC5_MOUSE

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Protein
Baculoviral IAP repeat-containing protein 5
Gene
Birc5, Api4, Iap4
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7 By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi57 – 571Zinc 1
Metal bindingi60 – 601Zinc 1
Metal bindingi76 – 761Zinc 2
Metal bindingi77 – 771Zinc 1
Metal bindingi80 – 801Zinc 2
Metal bindingi84 – 841Zinc 1

GO - Molecular functioni

  1. cofactor binding Source: UniProtKB
  2. cysteine-type endopeptidase inhibitor activity involved in apoptotic process Source: UniProtKB
  3. microtubule binding Source: UniProtKB
  4. protein binding Source: MGI
  5. protein homodimerization activity Source: UniProtKB
  6. tubulin binding Source: UniProtKB
  7. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. G2/M transition of mitotic cell cycle Source: UniProtKB
  2. cell division Source: UniProtKB
  3. chromosome segregation Source: UniProtKB-KW
  4. cytokinesis Source: UniProtKB
  5. establishment of chromosome localization Source: UniProtKB
  6. microtubule cytoskeleton organization Source: MGI
  7. mitotic nuclear division Source: UniProtKB-KW
  8. negative regulation of apoptotic process Source: UniProtKB
  9. negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
  10. negative regulation of neuron apoptotic process Source: MGI
  11. negative regulation of transcription, DNA-templated Source: UniProtKB
  12. positive regulation of exit from mitosis Source: UniProtKB
  13. positive regulation of mitotic cell cycle Source: UniProtKB
  14. protein complex localization Source: UniProtKB
  15. protein phosphorylation Source: UniProtKB
  16. regulation of insulin secretion involved in cellular response to glucose stimulus Source: MGI
  17. regulation of mitotic cell cycle Source: MGI
  18. regulation of type B pancreatic cell proliferation Source: MGI
  19. spindle checkpoint Source: UniProtKB
  20. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Repressor, Thiol protease inhibitor

Keywords - Biological processi

Apoptosis, Cell cycle, Cell division, Chromosome partition, Mitosis, Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_198961. Resolution of Sister Chromatid Cohesion.
REACT_207679. Separation of Sister Chromatids.

Protein family/group databases

MEROPSiI32.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Baculoviral IAP repeat-containing protein 5
Alternative name(s):
Apoptosis inhibitor 4
Apoptosis inhibitor survivin
TIAP
Gene namesi
Name:Birc5
Synonyms:Api4, Iap4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:1203517. Birc5.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity. Chromosome. Chromosomecentromere By similarity. Cytoplasmcytoskeletonspindle By similarity. Chromosomecentromerekinetochore By similarity. Midbody By similarity
Note: Localizes on chromosome arms and inner centromeres from prophase through metaphase. Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, localizes exclusively to the midbody. Colocalizes with AURKB at mitotic chromosomes. Acetylation at Lys-129 directs its localization to the nucleus by enhancing homodimerization and thereby inhibiting XPO1/CRM1-mediated nuclear export By similarity.

GO - Cellular componenti

  1. centriole Source: UniProtKB
  2. chromosome passenger complex Source: UniProtKB
  3. chromosome, centromeric region Source: UniProtKB
  4. condensed chromosome kinetochore Source: UniProtKB
  5. cytoplasm Source: UniProtKB
  6. cytoplasmic microtubule Source: UniProtKB
  7. cytosol Source: UniProtKB
  8. interphase microtubule organizing center Source: UniProtKB
  9. midbody Source: UniProtKB
  10. nucleus Source: UniProtKB
  11. spindle microtubule Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 140140Baculoviral IAP repeat-containing protein 5
PRO_0000122357Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei23 – 231N6-acetyllysine By similarity
Modified residuei34 – 341Phosphothreonine; by CDK11 Publication
Modified residuei48 – 481Phosphothreonine By similarity
Modified residuei90 – 901N6-acetyllysine By similarity
Modified residuei110 – 1101N6-acetyllysine By similarity
Modified residuei112 – 1121N6-acetyllysine By similarity
Modified residuei115 – 1151N6-acetyllysine By similarity
Modified residuei117 – 1171Phosphothreonine; by AURKB By similarity
Modified residuei129 – 1291N6-acetyllysine By similarity

Post-translational modificationi

Ubiquitinated by the Cul9-RING ubiquitin-protein ligase complex, leading to its degradation. Ubiquitination is required for centrosomal targeting By similarity.
In vitro phosphorylation at Thr-117 by AURKB prevents interaction with INCENP and localization to mitotic chromosomes By similarity.
Acetylation at Lys-129 results in its homodimerization, while deacetylation promotes the formation of monomers which heterodimerize with XPO1/CRM1 which facilitates its nuclear export. The acetylated form represses STAT3 transactivation. The dynamic equilibrium between its acetylation and deacetylation at Lys-129 determines its interaction with XPO1/CRM1, its subsequent subcellular localization, and its ability to inhibit STAT3 transactivation By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiO70201.
PRIDEiO70201.

PTM databases

PhosphoSiteiO70201.

Expressioni

Gene expression databases

ArrayExpressiO70201.
BgeeiO70201.
CleanExiMM_BIRC5.
GenevestigatoriO70201.

Interactioni

Subunit structurei

Monomer or homodimer. Exists as a homodimer in the apo state and as a monomer in the CPC-bound state. The monomer protects cells against apoptosis more efficiently than the dimer. Only the dimeric form is capable of enhancing tubulin stability in cells. When phosphorylated, interacts with LAMTOR5/HBXIP; the resulting complex binds pro-CASP9, as well as active CASP9, but much less efficiently. Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB and AURKC. Interacts with JTB. Interacts with CDCA8 and INCENP; interaction is direct. Interacts with EVI5. Interacts with GTP-bound RAN in both the S and M phases of the cell cycle. Interacts with USP9X. Interacts with tubulin. Interacts with BIRC2/c-IAP1. The acetylated form at Lys-129 interacts with STAT3. The monomeric form deacetylated at Lys-129 interacts with XPO1/CRM1. The monomeric form interacts with XIAP/BIRC4. Both the dimeric and monomeric form can interact with DIABLO/SMAC. Interacts with BIRC6/bruce By similarity.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi11 – 133
Helixi15 – 206
Beta strandi29 – 313
Helixi35 – 406
Beta strandi43 – 453
Beta strandi55 – 573
Turni58 – 603
Helixi73 – 797
Helixi85 – 884
Helixi93 – 953
Helixi98 – 11619

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1M4MX-ray2.80A1-140[»]
ProteinModelPortaliO70201.
SMRiO70201. Positions 7-118.

Miscellaneous databases

EvolutionaryTraceiO70201.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati18 – 8871BIR
Add
BLAST

Domaini

The BIR repeat is necessary and sufficient for LAMTOR5 binding By similarity.

Sequence similaritiesi

Belongs to the IAP family.
Contains 1 BIR repeat.

Phylogenomic databases

eggNOGiNOG271140.
GeneTreeiENSGT00510000047537.
HOGENOMiHOG000008032.
HOVERGENiHBG050690.
InParanoidiO70201.
KOiK08731.
OMAiNSPDIAM.
OrthoDBiEOG7SFJ01.
PhylomeDBiO70201.
TreeFamiTF342652.

Family and domain databases

Gene3Di1.10.1170.10. 1 hit.
InterProiIPR001370. BIR.
[Graphical view]
PfamiPF00653. BIR. 1 hit.
[Graphical view]
SMARTiSM00238. BIR. 1 hit.
[Graphical view]
PROSITEiPS50143. BIR_REPEAT_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O70201-1) [UniParc]FASTAAdd to Basket

Also known as: Survivin 140

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGAPALPQIW QLYLKNYRIA TFKNWPFLED CACTPERMAE AGFIHCPTEN    50
EPDLAQCFFC FKELEGWEPD DNPIEEHRKH SPGCAFLTVK KQMEELTVSE 100
FLKLDRQRAK NKIAKETNNK QKEFEETAKT TRQSIEQLAA 140
Length:140
Mass (Da):16,298
Last modified:August 1, 1998 - v1
Checksum:i26F5ABF501A6D83C
GO
Isoform 2 (identifier: O70201-2) [UniParc]FASTAAdd to Basket

Also known as: Survivin 121

The sequence of this isoform differs from the canonical sequence as follows:
     114-140: AKETNNKQKEFEETAKTTRQSIEQLAA → VCMIENKD

Show »
Length:121
Mass (Da):14,154
Checksum:i1E0EC7E01BA65585
GO
Isoform 3 (identifier: O70201-3) [UniParc]FASTAAdd to Basket

Also known as: Survivin 40

The sequence of this isoform differs from the canonical sequence as follows:
     38-40: MAE → RGA
     41-140: Missing.

Show »
Length:40
Mass (Da):4,670
Checksum:iFAD3C7E34A20518C
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei38 – 403MAE → RGA in isoform 3.
VSP_002455
Alternative sequencei41 – 140100Missing in isoform 3.
VSP_002456Add
BLAST
Alternative sequencei114 – 14027AKETN…EQLAA → VCMIENKD in isoform 2.
VSP_002457Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF077349 mRNA. Translation: AAD34225.1.
AB013819 mRNA. Translation: BAA28266.1.
AF115517 Genomic DNA. Translation: AAD26199.1.
AF115517 Genomic DNA. Translation: AAD26200.1.
AF115517 Genomic DNA. Translation: AAD26201.1.
BC004702 mRNA. Translation: AAH04702.1.
CCDSiCCDS25694.1. [O70201-1]
CCDS25695.1. [O70201-2]
RefSeqiNP_001012273.1. NM_001012273.1. [O70201-2]
NP_033819.1. NM_009689.2. [O70201-1]
UniGeneiMm.8552.

Genome annotation databases

EnsembliENSMUST00000081387; ENSMUSP00000079124; ENSMUSG00000017716. [O70201-1]
ENSMUST00000093906; ENSMUSP00000091433; ENSMUSG00000017716. [O70201-2]
GeneIDi11799.
KEGGimmu:11799.
UCSCiuc007mod.1. mouse. [O70201-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF077349 mRNA. Translation: AAD34225.1 .
AB013819 mRNA. Translation: BAA28266.1 .
AF115517 Genomic DNA. Translation: AAD26199.1 .
AF115517 Genomic DNA. Translation: AAD26200.1 .
AF115517 Genomic DNA. Translation: AAD26201.1 .
BC004702 mRNA. Translation: AAH04702.1 .
CCDSi CCDS25694.1. [O70201-1 ]
CCDS25695.1. [O70201-2 ]
RefSeqi NP_001012273.1. NM_001012273.1. [O70201-2 ]
NP_033819.1. NM_009689.2. [O70201-1 ]
UniGenei Mm.8552.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1M4M X-ray 2.80 A 1-140 [» ]
ProteinModelPortali O70201.
SMRi O70201. Positions 7-118.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi I32.005.

PTM databases

PhosphoSitei O70201.

Proteomic databases

MaxQBi O70201.
PRIDEi O70201.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000081387 ; ENSMUSP00000079124 ; ENSMUSG00000017716 . [O70201-1 ]
ENSMUST00000093906 ; ENSMUSP00000091433 ; ENSMUSG00000017716 . [O70201-2 ]
GeneIDi 11799.
KEGGi mmu:11799.
UCSCi uc007mod.1. mouse. [O70201-1 ]

Organism-specific databases

CTDi 332.
MGIi MGI:1203517. Birc5.

Phylogenomic databases

eggNOGi NOG271140.
GeneTreei ENSGT00510000047537.
HOGENOMi HOG000008032.
HOVERGENi HBG050690.
InParanoidi O70201.
KOi K08731.
OMAi NSPDIAM.
OrthoDBi EOG7SFJ01.
PhylomeDBi O70201.
TreeFami TF342652.

Enzyme and pathway databases

Reactomei REACT_198961. Resolution of Sister Chromatid Cohesion.
REACT_207679. Separation of Sister Chromatids.

Miscellaneous databases

EvolutionaryTracei O70201.
NextBioi 279641.
PROi O70201.
SOURCEi Search...

Gene expression databases

ArrayExpressi O70201.
Bgeei O70201.
CleanExi MM_BIRC5.
Genevestigatori O70201.

Family and domain databases

Gene3Di 1.10.1170.10. 1 hit.
InterProi IPR001370. BIR.
[Graphical view ]
Pfami PF00653. BIR. 1 hit.
[Graphical view ]
SMARTi SM00238. BIR. 1 hit.
[Graphical view ]
PROSITEi PS50143. BIR_REPEAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mammalian inhibitor of apoptosis (IAP) homolog."
    Uren A.G., Vaux D.L.
    Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. Kobayashi K., Otaki M., Ogasawara T., Tokuhisa T.
    Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Embryo.
  3. "Three differentially expressed survivin cDNA variants encode proteins with distinct antiapoptotic functions."
    Conway E.M., Pollefeyt S., Cornelissen J., DeBaere I., Steiner-Mosonyi M., Ong K., Baens M., Collen D., Schuh A.C.
    Blood 95:1435-1442(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1; 2 AND 3).
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  5. "Crystal structure and mutagenic analysis of the inhibitor-of-apoptosis protein survivin."
    Muchmore S.W., Chen J., Jakob C., Zakula D., Matayoshi E.D., Wu W., Zhang H., Li F., Ng S.C., Altieri D.C.
    Mol. Cell 6:173-182(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 7-118, PHOSPHORYLATION AT THR-34.

Entry informationi

Entry nameiBIRC5_MOUSE
AccessioniPrimary (citable) accession number: O70201
Secondary accession number(s): Q923F7, Q9WU53, Q9WU54
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: September 3, 2014
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi