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Protein

Allograft inflammatory factor 1

Gene

Aif1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.5 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi58 – 691PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi94 – 1052Add BLAST12

GO - Molecular functioni

  • actin filament binding Source: UniProtKB
  • calcium ion binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Actin-binding, Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Allograft inflammatory factor 1
Short name:
AIF-1
Alternative name(s):
Ionized calcium-binding adapter molecule 1
Gene namesi
Name:Aif1
Synonyms:Iba1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1343098. Aif1.

Subcellular locationi

  • Cytoplasmcytoskeleton 1 Publication
  • Cell projectionruffle membrane 2 Publications; Peripheral membrane protein; Cytoplasmic side
  • Cell projectionphagocytic cup 2 Publications

  • Note: Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.1 Publication

GO - Cellular componenti

  • actin filament Source: MGI
  • cytoplasm Source: UniProtKB
  • cytosol Source: UniProtKB
  • lamellipodium Source: UniProtKB
  • nucleus Source: UniProtKB
  • perikaryon Source: Ensembl
  • perinuclear region of cytoplasm Source: MGI
  • phagocytic cup Source: UniProtKB
  • ruffle Source: MGI
  • ruffle membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000738672 – 147Allograft inflammatory factor 1Add BLAST146

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei11N6-acetyllysineBy similarity1

Post-translational modificationi

Phosphorylated on serine residues.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiO70200.
PRIDEiO70200.

PTM databases

iPTMnetiO70200.
PhosphoSitePlusiO70200.

Expressioni

Tissue specificityi

Abundantly expressed in the testis, moderately in the spleen and lymph nodes and at low levels in the liver and thymus. Detected in macrophages.1 Publication

Gene expression databases

BgeeiENSMUSG00000024397.
CleanExiMM_AIF1.
ExpressionAtlasiO70200. baseline and differential.
GenevisibleiO70200. MM.

Interactioni

Subunit structurei

Homodimer (Potential). Monomer. Interacts with LCP1.Curated2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198041. 2 interactors.
STRINGi10090.ENSMUSP00000025257.

Structurei

Secondary structure

1147
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi18 – 31Combined sources14
Helixi35 – 38Combined sources4
Helixi43 – 54Combined sources12
Beta strandi63 – 66Combined sources4
Helixi67 – 76Combined sources10
Helixi83 – 93Combined sources11
Helixi103 – 110Combined sources8
Helixi114 – 123Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WY9X-ray2.10A1-147[»]
ProteinModelPortaliO70200.
SMRiO70200.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO70200.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 80EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini81 – 115EF-hand 2; degeneratePROSITE-ProRule annotationAdd BLAST35

Sequence similaritiesi

Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410KCUI. Eukaryota.
ENOG411206J. LUCA.
GeneTreeiENSGT00390000013846.
HOGENOMiHOG000231928.
HOVERGENiHBG004002.
InParanoidiO70200.
KOiK18617.
OMAiAFKKKYM.
OrthoDBiEOG091G0ZI9.
PhylomeDBiO70200.
TreeFamiTF320736.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50222. EF_HAND_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O70200-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQSRDLQGG KAFGLLKAQQ EERLEGINKQ FLDDPKYSND EDLPSKLEAF
60 70 80 90 100
KVKYMEFDLN GNGDIDIMSL KRMLEKLGVP KTHLELKRLI REVSSGSEET
110 120 130 140
FSYSDFLRMM LGKRSAILRM ILMYEEKNKE HKRPTGPPAK KAISELP
Length:147
Mass (Da):16,911
Last modified:August 1, 1998 - v1
Checksum:iD8974825C153D3CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB013745 mRNA. Translation: BAA28216.1.
AB036423 Genomic DNA. Translation: BAB20758.1.
D86382 mRNA. Translation: BAA86387.1.
AF074959 mRNA. Translation: AAC25604.1.
U82792 Genomic DNA. Translation: AAC24189.1.
AK006184 mRNA. Translation: BAB24445.1.
AK006562 mRNA. Translation: BAB24654.1.
AF109719 Genomic DNA. Translation: AAC82481.1.
BC021539 mRNA. Translation: AAH21539.1.
CCDSiCCDS28689.1.
RefSeqiNP_062340.1. NM_019467.2.
XP_006523566.1. XM_006523503.3.
XP_006523567.1. XM_006523504.3.
UniGeneiMm.10747.

Genome annotation databases

EnsembliENSMUST00000025257; ENSMUSP00000025257; ENSMUSG00000024397.
ENSMUST00000172693; ENSMUSP00000134214; ENSMUSG00000024397.
ENSMUST00000173324; ENSMUSP00000133709; ENSMUSG00000024397.
GeneIDi11629.
KEGGimmu:11629.
UCSCiuc008cgl.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB013745 mRNA. Translation: BAA28216.1.
AB036423 Genomic DNA. Translation: BAB20758.1.
D86382 mRNA. Translation: BAA86387.1.
AF074959 mRNA. Translation: AAC25604.1.
U82792 Genomic DNA. Translation: AAC24189.1.
AK006184 mRNA. Translation: BAB24445.1.
AK006562 mRNA. Translation: BAB24654.1.
AF109719 Genomic DNA. Translation: AAC82481.1.
BC021539 mRNA. Translation: AAH21539.1.
CCDSiCCDS28689.1.
RefSeqiNP_062340.1. NM_019467.2.
XP_006523566.1. XM_006523503.3.
XP_006523567.1. XM_006523504.3.
UniGeneiMm.10747.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WY9X-ray2.10A1-147[»]
ProteinModelPortaliO70200.
SMRiO70200.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198041. 2 interactors.
STRINGi10090.ENSMUSP00000025257.

PTM databases

iPTMnetiO70200.
PhosphoSitePlusiO70200.

Proteomic databases

PaxDbiO70200.
PRIDEiO70200.

Protocols and materials databases

DNASUi11629.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025257; ENSMUSP00000025257; ENSMUSG00000024397.
ENSMUST00000172693; ENSMUSP00000134214; ENSMUSG00000024397.
ENSMUST00000173324; ENSMUSP00000133709; ENSMUSG00000024397.
GeneIDi11629.
KEGGimmu:11629.
UCSCiuc008cgl.1. mouse.

Organism-specific databases

CTDi199.
MGIiMGI:1343098. Aif1.

Phylogenomic databases

eggNOGiENOG410KCUI. Eukaryota.
ENOG411206J. LUCA.
GeneTreeiENSGT00390000013846.
HOGENOMiHOG000231928.
HOVERGENiHBG004002.
InParanoidiO70200.
KOiK18617.
OMAiAFKKKYM.
OrthoDBiEOG091G0ZI9.
PhylomeDBiO70200.
TreeFamiTF320736.

Miscellaneous databases

EvolutionaryTraceiO70200.
PROiO70200.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024397.
CleanExiMM_AIF1.
ExpressionAtlasiO70200. baseline and differential.
GenevisibleiO70200. MM.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50222. EF_HAND_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAIF1_MOUSE
AccessioniPrimary (citable) accession number: O70200
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.