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Reviewed, UniProtKB/Swiss-Prot O70199 (UGDH_RAT)

Last modified February 9, 2010. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    UDP-glucose 6-dehydrogenase
      Short name=UDP-Glc dehydrogenase
      Short name=UDP-GlcDH
      Short name=UDPGDH
    EC=1.1.1.22
Gene names
Name: Ugdh
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length493 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate.

Catalytic activity

UDP-glucose + 2 NAD+ + H2O = UDP-glucuronate + 2 NADH.

Pathway

Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1.

Subunit structure

Homohexamer By similarity.

Sequence similarities

Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.

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Ontologies

Keywords
   LigandNAD
   Molecular functionOxidoreductase
   PTMAcetylation
Phosphoprotein
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytosol

Inferred from direct assay. Source: RGD

   Molecular functionNAD or NADH binding

Inferred from electronic annotation. Source: InterPro

UDP-glucose 6-dehydrogenase activity

Inferred from direct assay. Source: RGD

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 493493UDP-glucose 6-dehydrogenase
PRO_0000074062

Regions

Nucleotide binding11 – 166NAD By similarity
Nucleotide binding89 – 935NAD By similarity
Nucleotide binding130 – 1312NAD By similarity
Nucleotide binding276 – 2794NAD By similarity
Region161 – 1655Substrate binding By similarity
Region220 – 2278Substrate binding By similarity
Region260 – 27314Substrate binding By similarity
Region338 – 3392Substrate binding By similarity

Sites

Active site2761Nucleophile By similarity
Binding site361NAD By similarity
Binding site411NAD By similarity
Binding site1651NAD By similarity
Binding site3461NAD By similarity
Binding site4421Substrate By similarity

Amino acid modifications

Modified residue1071N6-acetyllysine By similarity
Modified residue1731N6-acetyllysine By similarity
Modified residue4341N6-acetyllysine By similarity
Modified residue4731Phosphotyrosine By similarity
Modified residue4741Phosphothreonine Ref.2 Ref.3

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Sequences

Sequence LengthMass (Da)Tools
O70199-1 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: F435D1F9AC8307EF

FASTA49354,892
        10         20         30         40         50         60 
MVEIKKICCI GAGYVGGPTC SVIARMCPEI RVTVVDVNEA RINAWNSPTL PIYEPGLKEV 

        70         80         90        100        110        120 
VESCRGKNLF FSTNIDDAIR EADLVFISVN TPTKTYGMGK GRAADLKYIE ACARRIVQNS 

       130        140        150        160        170        180 
NGYKIVTEKS TVPVRAAESI RRIFDANTKP NLNLQVLSNP EFLAEGTAIK DLKNPDRVLI 

       190        200        210        220        230        240 
GGDETPEGQR AVQALCAVYE HWVPKEKILT TNTWSSELSK LAANAFLAQR ISSINSISAL 

       250        260        270        280        290        300 
CESTGADVEE VATAIGMDQR IGNKFLKASV GFGGGCFQKD VLNLVYLCEA LNLPEVARYW 

       310        320        330        340        350        360 
QQVIDMNDYQ RRRFASRIID SLFNTVTDKK IAILGFAFKK DTGDTRESSS IYISKYLMDE 

       370        380        390        400        410        420 
GAHLHIYDPK VPREQIVVDL SHPGVSADDQ VSRLVTISKD PYEACDGAHA LVICTEWDMF 

       430        440        450        460        470        480 
KELDYERIHK RMLKPAFIFD GRRVLDGLHN ELQTIGFQIE TIGKKVSSKR IPYTPGEIPK 

       490 
FSLQDPPNKK PKV

« Hide

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References

« Hide 'large scale' references
[1]"cDNA cloning of rat UDP-glucose dehydrogenase."
Kobayashi T., Yokota H., Yuasa A.
Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Wistar.
Tissue: Liver.
[2]"Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS."
Moser K., White F.M.
J. Proteome Res. 5:98-104(2006) [PubMed: 16396499] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-474, MASS SPECTROMETRY.
Tissue: Liver.
[3]"Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed: 16641100] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-474, MASS SPECTROMETRY.
Tissue: Kidney.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB013732 mRNA. Translation: BAA28215.1.
IPIIPI00195803.
RefSeqNP_112615.1.
UniGeneRn.3967

3D structure databases

SMRO70199. Positions 1-466.
ModBaseSearch...

Protein-protein interaction databases

STRINGO70199.

PTM databases

PhosphoSiteO70199.

Proteomic databases

PRIDEO70199.

Genome annotation databases

EnsemblENSRNOT00000003691; ENSRNOP00000003691; ENSRNOG00000002643; Rattus norvegicus. [Genome view]
GeneID83472.
KEGGrno:83472.
UCSCNM_031325. rat.

Organism-specific databases

CTD83472.
RGD621879. Ugdh.

Phylogenomic databases

eggNOGroNOG08820.
HOVERGENO70199.
InParanoidO70199.

Enzyme and pathway databases

BRENDA1.1.1.22. 248.

Gene expression databases

ArrayExpressO70199.
GenevestigatorO70199.
GermOnlineENSRNOG00000002643. Rattus norvegicus.

Family and domain databases

InterProIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR017476. Nucleotide_sugar_DH.
IPR014027. UDP-Glc/GDP-Man_DH_C.
IPR014026. UDP-Glc/GDP-Man_DH_dimer.
IPR014028. UDP-Glc/GDP-Man_DH_dimer-bd.
IPR001732. UDP-Glc/GDP-Man_DH_N.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
G3DSA:3.40.50.1870. UDP-Glc/GDP-Man_DH_C. 1 hit.
PANTHERPTHR11374. UDPG_MGDP_DH_Creg. 1 hit.
PfamPF00984. UDPG_MGDP_dh. 1 hit.
PF03720. UDPG_MGDP_dh_C. 1 hit.
PF03721. UDPG_MGDP_dh_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR03026. NDP-sugDHase. 1 hit.
ProtoNetSearch...

Other Resources

NextBio615885.

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Entry information

Entry nameUGDH_RAT
AccessionPrimary (citable) accession number: O70199
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: February 9, 2010
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents