Reviewed, UniProtKB/Swiss-Prot O70199 (UGDH_RAT)

Names and origin

Protein names

Recommended name:
    UDP-glucose 6-dehydrogenase
      Short name=UDP-Glc dehydrogenase
      Short name=UDP-GlcDH
      Short name=UDPGDH
    EC=1.1.1.22

Gene names

Name:

Ugdh

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate.
Catalytic activity	UDP-glucose + 2 NAD(+) + H(2)O = UDP-glucuronate + 2 NADH.
Pathway	Nucleotide-sugar biosynthesis; UDP-glucuronate biosynthesis; UDP-glucuronate from UDP-glucose: step 1/1.
Subunit structure	Homohexamer By similarity.
Sequence similarities	Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro UDP-glucose 6-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 493	493	UDP-glucose 6-dehydrogenase		PRO_0000074062
Regions
	Nucleotide binding	6 – 23	18	NAD By similarity
	Region	162 – 165	4	Substrate binding By similarity
Sites
	Active site	276	1	Nucleophile By similarity
	Binding site	36	1	NAD By similarity
	Binding site	41	1	NAD By similarity
	Binding site	93	1	NAD By similarity
	Binding site	131	1	NAD; via amide nitrogen By similarity
	Binding site	165	1	NAD By similarity
	Binding site	220	1	Substrate By similarity
	Binding site	273	1	Substrate; via amide nitrogen By similarity
	Binding site	279	1	NAD By similarity
	Binding site	339	1	Substrate By similarity
	Binding site	346	1	NAD By similarity
Amino acid modifications
	Modified residue	473	1	Phosphotyrosine By similarity
	Modified residue	474	1	Phosphothreonine

Sequences

	Sequence		Length	Mass (Da)
	O70199-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: F435D1F9AC8307EF	FASTA	493	54,892
	10 20 30 40 50 60 MVEIKKICCI GAGYVGGPTC SVIARMCPEI RVTVVDVNEA RINAWNSPTL PIYEPGLKEV 70 80 90 100 110 120 VESCRGKNLF FSTNIDDAIR EADLVFISVN TPTKTYGMGK GRAADLKYIE ACARRIVQNS 130 140 150 160 170 180 NGYKIVTEKS TVPVRAAESI RRIFDANTKP NLNLQVLSNP EFLAEGTAIK DLKNPDRVLI 190 200 210 220 230 240 GGDETPEGQR AVQALCAVYE HWVPKEKILT TNTWSSELSK LAANAFLAQR ISSINSISAL 250 260 270 280 290 300 CESTGADVEE VATAIGMDQR IGNKFLKASV GFGGGCFQKD VLNLVYLCEA LNLPEVARYW 310 320 330 340 350 360 QQVIDMNDYQ RRRFASRIID SLFNTVTDKK IAILGFAFKK DTGDTRESSS IYISKYLMDE 370 380 390 400 410 420 GAHLHIYDPK VPREQIVVDL SHPGVSADDQ VSRLVTISKD PYEACDGAHA LVICTEWDMF 430 440 450 460 470 480 KELDYERIHK RMLKPAFIFD GRRVLDGLHN ELQTIGFQIE TIGKKVSSKR IPYTPGEIPK 490 FSLQDPPNKK PKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning of rat UDP-glucose dehydrogenase." Kobayashi T., Yokota H., Yuasa A. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Liver.
[2]	"Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS." Moser K., White F.M. J. Proteome Res. 5:98-104(2006) [PubMed: 16396499] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-474, MASS SPECTROMETRY. Tissue: Liver.
[3]	"Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites." Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A. Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed: 16641100] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-474, MASS SPECTROMETRY. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB013732 mRNA. Translation: BAA28215.1.
RefSeq	NP_112615.1.
UniGene	Rn.3967
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	O70199.
Genome annotation databases
Ensembl	ENSRNOG00000002643. Rattus norvegicus. [Contig view]
GeneID	83472.
KEGG	rno:83472.
Organism-specific databases
RGD	621879. Ugdh.
Phylogenomic databases
HOVERGEN	O70199.
Gene expression databases
ArrayExpress	O70199.
GermOnline	ENSRNOG00000002643. Rattus norvegicus.
Family and domain databases
InterPro	IPR016040. NAD(P)-bd. IPR017476. Nucleotide_sugar_DH. IPR014027. UDP-Glc/GDP-Man_DHase_C. IPR014026. UDP-Glc/GDP-Man_DHase_dimer. IPR014028. UDP-Glc/GDP-Man_DHase_dimer-bd. IPR001732. UDP-Glc/GDP-Man_DHase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:3.40.50.1870. UDP-Glc/GDP-Man_DH_C. 1 hit.
PANTHER	PTHR11374. UDPG_MGDP_DH_Creg. 1 hit.
Pfam	PF00984. UDPG_MGDP_dh. 1 hit. PF03720. UDPG_MGDP_dh_C. 1 hit. PF03721. UDPG_MGDP_dh_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	615885.

Entry information

Entry name

UGDH_RAT

Accession

Primary (citable) accession number: O70199

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	November 25, 2008
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)