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Protein

Prolyl endopeptidase

Gene

Prep

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Has high activity on the succinyl- (suc-) peptide-4-methylcoumaryl-7-amide (MCA) substrates suc-Gly-Pro-Leu-Gly-Pro-MCA, suc-Gly-Pro-MCA and suc-Ala-Ala-Ala-MCA.1 Publication

Catalytic activityi

Hydrolysis of Pro-|-Xaa >> Ala-|-Xaa in oligopeptides.1 Publication

Enzyme regulationi

Inhibited by DFP, Z-Pro-prolinal and poststatin, but not by PMSF, SBTI, EDTA, leupeptin, E-64 and pepstatin.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei554Charge relay systemPROSITE-ProRule annotationBy similarity1
Active sitei641Charge relay systemPROSITE-ProRule annotationBy similarity1
Active sitei680Charge relay systemPROSITE-ProRule annotationBy similarity1

GO - Molecular functioni

  • endopeptidase activity Source: RGD
  • peptide binding Source: RGD
  • serine-type endopeptidase activity Source: GO_Central
  • serine-type exopeptidase activity Source: InterPro

GO - Biological processi

  • proteolysis Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.26. 5301.

Protein family/group databases

ESTHERiratno-RPOP. S9N_PPCE_Peptidase_S9.
MEROPSiS09.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Prolyl endopeptidaseBy similarity (EC:3.4.21.26)
Short name:
PEBy similarity
Alternative name(s):
Post-proline cleaving enzymeBy similarity
rPop
Gene namesi
Name:PrepImported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620841. Prep.

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

  • cytosol Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4035.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003656371 – 710Prolyl endopeptidaseAdd BLAST710

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei157N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiO70196.

PTM databases

iPTMnetiO70196.
PhosphoSitePlusiO70196.

Expressioni

Tissue specificityi

Expressed in all tissues tested: uterus, kidney, heart, lung, small intestine, smooth muscle, liver, spleen, thymus, adrenal, pituitary and whole brain.1 Publication

Developmental stagei

In the estrous cycle, expression and activity are highest in the luteal phase.1 Publication

Interactioni

Chemistry databases

BindingDBiO70196.

Structurei

3D structure databases

ProteinModelPortaliO70196.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S9A family.Sequence analysis

Phylogenomic databases

HOGENOMiHOG000238967.
HOVERGENiHBG007251.
InParanoidiO70196.
KOiK01322.
PhylomeDBiO70196.

Family and domain databases

Gene3Di2.130.10.120. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002471. Pept_S9_AS.
IPR023302. Pept_S9A_N.
IPR001375. Peptidase_S9.
IPR002470. Peptidase_S9A.
[Graphical view]
PANTHERiPTHR11757. PTHR11757. 1 hit.
PfamiPF00326. Peptidase_S9. 1 hit.
PF02897. Peptidase_S9_N. 1 hit.
[Graphical view]
PRINTSiPR00862. PROLIGOPTASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00708. PRO_ENDOPEP_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O70196-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSFQYPDVY RDETSVQDYH GHKICDPYAW LEDPDSEQTK AFVEAQNKIT
60 70 80 90 100
VPFLEQCPIR GLYKERMTEL YDYPKYSCHF KKGKRYFYFY NTGLQNQRVL
110 120 130 140 150
YVQDSLEGEA RVFLDPNTLS DDGTVALRGY AFSEDGEYFA YGLSASGSDW
160 170 180 190 200
VTIKFMKVDG AKELPDVLER VKFTCMAWTH DGKGMFYNSY PQQDGKSDGT
210 220 230 240 250
ETSTNLHQKL CYHVLGTDQS EDVLCAEFPD EPKWMGGAEL SDDGRYVLLS
260 270 280 290 300
IWEGCDPVNR LWYCDLQQGS NGINGILKWV KLIDNFEGEY DYITNEGTVF
310 320 330 340 350
TFKTNRNSPN YRLINIDFTD PDESKWKVLV PEHEKDVLEW VACVRSNFLV
360 370 380 390 400
LCYLRNVKNI LQLHDLTTGA LLKTFPLDVG SVVGYSGRKK DSEIFYQFTS
410 420 430 440 450
FLSPGVIYHC DLTREELEPR VFREVTVKGI DASDYQTIQV FYPSKDGTKI
460 470 480 490 500
PMFIVHKKGI KLDGSHPAFL YGYGGFNISI TPNYSVSRLI FVRHMGGVLA
510 520 530 540 550
VANIRGGGEY GETWHKGGIL ANKQNCFDDF QCAAEYLIKE GYTTSKRLTI
560 570 580 590 600
NGGSNGGLLV AACANQRPDL FGCVIAQVGV MDMLKFHKFT IGHAWTTDYG
610 620 630 640 650
CSDSKQHFEW LLKYSPLHNV KLPEADDIQY PSMLLLTADH DDRVVPLHSL
660 670 680 690 700
KFIATLQYIV GRSRKQSNPL LIHVDTKAGH GPGKPTAKVI EEVSDMFAFI
710
ARCLNIEWIQ
Length:710
Mass (Da):80,742
Last modified:August 1, 1998 - v1
Checksum:iEE6A6AD78D79174C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012759 mRNA. Translation: BAA25544.1.
RefSeqiNP_112614.1. NM_031324.1.
UniGeneiRn.11058.

Genome annotation databases

GeneIDi83471.
KEGGirno:83471.
UCSCiRGD:620841. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012759 mRNA. Translation: BAA25544.1.
RefSeqiNP_112614.1. NM_031324.1.
UniGeneiRn.11058.

3D structure databases

ProteinModelPortaliO70196.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiO70196.
ChEMBLiCHEMBL4035.

Protein family/group databases

ESTHERiratno-RPOP. S9N_PPCE_Peptidase_S9.
MEROPSiS09.001.

PTM databases

iPTMnetiO70196.
PhosphoSitePlusiO70196.

Proteomic databases

PRIDEiO70196.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi83471.
KEGGirno:83471.
UCSCiRGD:620841. rat.

Organism-specific databases

CTDi5550.
RGDi620841. Prep.

Phylogenomic databases

HOGENOMiHOG000238967.
HOVERGENiHBG007251.
InParanoidiO70196.
KOiK01322.
PhylomeDBiO70196.

Enzyme and pathway databases

BRENDAi3.4.21.26. 5301.

Miscellaneous databases

PROiO70196.

Family and domain databases

Gene3Di2.130.10.120. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002471. Pept_S9_AS.
IPR023302. Pept_S9A_N.
IPR001375. Peptidase_S9.
IPR002470. Peptidase_S9A.
[Graphical view]
PANTHERiPTHR11757. PTHR11757. 1 hit.
PfamiPF00326. Peptidase_S9. 1 hit.
PF02897. Peptidase_S9_N. 1 hit.
[Graphical view]
PRINTSiPR00862. PROLIGOPTASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00708. PRO_ENDOPEP_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPCE_RAT
AccessioniPrimary (citable) accession number: O70196
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.