Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Prolyl endopeptidase

Gene

Prep

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Has high activity on the succinyl- (suc-) peptide-4-methylcoumaryl-7-amide (MCA) substrates suc-Gly-Pro-Leu-Gly-Pro-MCA, suc-Gly-Pro-MCA and suc-Ala-Ala-Ala-MCA.1 Publication

Catalytic activityi

Hydrolysis of Pro-|-Xaa >> Ala-|-Xaa in oligopeptides.1 Publication

Enzyme regulationi

Inhibited by DFP, Z-Pro-prolinal and poststatin, but not by PMSF, SBTI, EDTA, leupeptin, E-64 and pepstatin.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei554Charge relay systemPROSITE-ProRule annotationBy similarity1
Active sitei641Charge relay systemPROSITE-ProRule annotationBy similarity1
Active sitei680Charge relay systemPROSITE-ProRule annotationBy similarity1

GO - Molecular functioni

  • endopeptidase activity Source: RGD
  • peptide binding Source: RGD
  • serine-type endopeptidase activity Source: InterPro
  • serine-type exopeptidase activity Source: InterPro

GO - Biological processi

  • proteolysis Source: RGD

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.26 5301

Protein family/group databases

ESTHERiratno-RPOP S9N_PPCE_Peptidase_S9
MEROPSiS09.001

Names & Taxonomyi

Protein namesi
Recommended name:
Prolyl endopeptidaseBy similarity (EC:3.4.21.26)
Short name:
PEBy similarity
Alternative name(s):
Post-proline cleaving enzymeBy similarity
rPop
Gene namesi
Name:PrepImported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620841 Prep

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4035

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003656371 – 710Prolyl endopeptidaseAdd BLAST710

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei157N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiO70196

PTM databases

iPTMnetiO70196
PhosphoSitePlusiO70196

Expressioni

Tissue specificityi

Expressed in all tissues tested: uterus, kidney, heart, lung, small intestine, smooth muscle, liver, spleen, thymus, adrenal, pituitary and whole brain.1 Publication

Developmental stagei

In the estrous cycle, expression and activity are highest in the luteal phase.1 Publication

Interactioni

Chemistry databases

BindingDBiO70196

Structurei

3D structure databases

ProteinModelPortaliO70196
SMRiO70196
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S9A family.Sequence analysis

Phylogenomic databases

HOGENOMiHOG000238967
HOVERGENiHBG007251
InParanoidiO70196
KOiK01322
PhylomeDBiO70196

Family and domain databases

Gene3Di3.40.50.1820, 2 hits
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR002471 Pept_S9_AS
IPR023302 Pept_S9A_N
IPR001375 Peptidase_S9
IPR002470 Peptidase_S9A
PfamiView protein in Pfam
PF00326 Peptidase_S9, 1 hit
PF02897 Peptidase_S9_N, 1 hit
PRINTSiPR00862 PROLIGOPTASE
SUPFAMiSSF53474 SSF53474, 1 hit
PROSITEiView protein in PROSITE
PS00708 PRO_ENDOPEP_SER, 1 hit

Sequencei

Sequence statusi: Complete.

O70196-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSFQYPDVY RDETSVQDYH GHKICDPYAW LEDPDSEQTK AFVEAQNKIT
60 70 80 90 100
VPFLEQCPIR GLYKERMTEL YDYPKYSCHF KKGKRYFYFY NTGLQNQRVL
110 120 130 140 150
YVQDSLEGEA RVFLDPNTLS DDGTVALRGY AFSEDGEYFA YGLSASGSDW
160 170 180 190 200
VTIKFMKVDG AKELPDVLER VKFTCMAWTH DGKGMFYNSY PQQDGKSDGT
210 220 230 240 250
ETSTNLHQKL CYHVLGTDQS EDVLCAEFPD EPKWMGGAEL SDDGRYVLLS
260 270 280 290 300
IWEGCDPVNR LWYCDLQQGS NGINGILKWV KLIDNFEGEY DYITNEGTVF
310 320 330 340 350
TFKTNRNSPN YRLINIDFTD PDESKWKVLV PEHEKDVLEW VACVRSNFLV
360 370 380 390 400
LCYLRNVKNI LQLHDLTTGA LLKTFPLDVG SVVGYSGRKK DSEIFYQFTS
410 420 430 440 450
FLSPGVIYHC DLTREELEPR VFREVTVKGI DASDYQTIQV FYPSKDGTKI
460 470 480 490 500
PMFIVHKKGI KLDGSHPAFL YGYGGFNISI TPNYSVSRLI FVRHMGGVLA
510 520 530 540 550
VANIRGGGEY GETWHKGGIL ANKQNCFDDF QCAAEYLIKE GYTTSKRLTI
560 570 580 590 600
NGGSNGGLLV AACANQRPDL FGCVIAQVGV MDMLKFHKFT IGHAWTTDYG
610 620 630 640 650
CSDSKQHFEW LLKYSPLHNV KLPEADDIQY PSMLLLTADH DDRVVPLHSL
660 670 680 690 700
KFIATLQYIV GRSRKQSNPL LIHVDTKAGH GPGKPTAKVI EEVSDMFAFI
710
ARCLNIEWIQ
Length:710
Mass (Da):80,742
Last modified:August 1, 1998 - v1
Checksum:iEE6A6AD78D79174C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012759 mRNA Translation: BAA25544.1
RefSeqiNP_112614.1, NM_031324.1
UniGeneiRn.11058

Genome annotation databases

GeneIDi83471
KEGGirno:83471
UCSCiRGD:620841 rat

Similar proteinsi

Entry informationi

Entry nameiPPCE_RAT
AccessioniPrimary (citable) accession number: O70196
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: August 1, 1998
Last modified: May 23, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health