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Protein

Prolyl endopeptidase

Gene

Prep

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Has high activity on the succinyl- (suc-) peptide-4-methylcoumaryl-7-amide (MCA) substrates suc-Gly-Pro-Leu-Gly-Pro-MCA, suc-Gly-Pro-MCA and suc-Ala-Ala-Ala-MCA.1 Publication

Catalytic activityi

Hydrolysis of Pro-|-Xaa >> Ala-|-Xaa in oligopeptides.1 Publication

Enzyme regulationi

Inhibited by DFP, Z-Pro-prolinal and poststatin, but not by PMSF, SBTI, EDTA, leupeptin, E-64 and pepstatin.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei554 – 5541Charge relay systemBy similarityPROSITE-ProRule annotation
Active sitei641 – 6411Charge relay systemBy similarityPROSITE-ProRule annotation
Active sitei680 – 6801Charge relay systemBy similarityPROSITE-ProRule annotation

GO - Molecular functioni

  1. endopeptidase activity Source: RGD
  2. peptide binding Source: RGD
  3. serine-type endopeptidase activity Source: InterPro
  4. serine-type exopeptidase activity Source: InterPro

GO - Biological processi

  1. proteolysis Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS09.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Prolyl endopeptidaseBy similarity (EC:3.4.21.26)
Short name:
PEBy similarity
Alternative name(s):
Post-proline cleaving enzymeBy similarity
rPop
Gene namesi
Name:PrepImported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620841. Prep.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytosol Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 710710Prolyl endopeptidasePRO_0000365637Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei157 – 1571N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO70196.
PRIDEiO70196.

PTM databases

PhosphoSiteiO70196.

Expressioni

Tissue specificityi

Expressed in all tissues tested: uterus, kidney, heart, lung, small intestine, smooth muscle, liver, spleen, thymus, adrenal, pituitary and whole brain.1 Publication

Developmental stagei

In the estrous cycle, expression and activity are highest in the luteal phase.1 Publication

Gene expression databases

GenevestigatoriO70196.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000360.

Structurei

3D structure databases

ProteinModelPortaliO70196.
SMRiO70196. Positions 1-709.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S9A family.Sequence Analysis

Phylogenomic databases

eggNOGiCOG1505.
HOGENOMiHOG000238967.
HOVERGENiHBG007251.
InParanoidiO70196.
KOiK01322.
PhylomeDBiO70196.

Family and domain databases

Gene3Di2.130.10.120. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002471. Pept_S9_AS.
IPR023302. Pept_S9A_N.
IPR001375. Peptidase_S9.
IPR002470. Peptidase_S9A.
[Graphical view]
PANTHERiPTHR11757. PTHR11757. 1 hit.
PfamiPF00326. Peptidase_S9. 1 hit.
PF02897. Peptidase_S9_N. 1 hit.
[Graphical view]
PRINTSiPR00862. PROLIGOPTASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00708. PRO_ENDOPEP_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O70196-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLSFQYPDVY RDETSVQDYH GHKICDPYAW LEDPDSEQTK AFVEAQNKIT
60 70 80 90 100
VPFLEQCPIR GLYKERMTEL YDYPKYSCHF KKGKRYFYFY NTGLQNQRVL
110 120 130 140 150
YVQDSLEGEA RVFLDPNTLS DDGTVALRGY AFSEDGEYFA YGLSASGSDW
160 170 180 190 200
VTIKFMKVDG AKELPDVLER VKFTCMAWTH DGKGMFYNSY PQQDGKSDGT
210 220 230 240 250
ETSTNLHQKL CYHVLGTDQS EDVLCAEFPD EPKWMGGAEL SDDGRYVLLS
260 270 280 290 300
IWEGCDPVNR LWYCDLQQGS NGINGILKWV KLIDNFEGEY DYITNEGTVF
310 320 330 340 350
TFKTNRNSPN YRLINIDFTD PDESKWKVLV PEHEKDVLEW VACVRSNFLV
360 370 380 390 400
LCYLRNVKNI LQLHDLTTGA LLKTFPLDVG SVVGYSGRKK DSEIFYQFTS
410 420 430 440 450
FLSPGVIYHC DLTREELEPR VFREVTVKGI DASDYQTIQV FYPSKDGTKI
460 470 480 490 500
PMFIVHKKGI KLDGSHPAFL YGYGGFNISI TPNYSVSRLI FVRHMGGVLA
510 520 530 540 550
VANIRGGGEY GETWHKGGIL ANKQNCFDDF QCAAEYLIKE GYTTSKRLTI
560 570 580 590 600
NGGSNGGLLV AACANQRPDL FGCVIAQVGV MDMLKFHKFT IGHAWTTDYG
610 620 630 640 650
CSDSKQHFEW LLKYSPLHNV KLPEADDIQY PSMLLLTADH DDRVVPLHSL
660 670 680 690 700
KFIATLQYIV GRSRKQSNPL LIHVDTKAGH GPGKPTAKVI EEVSDMFAFI
710
ARCLNIEWIQ
Length:710
Mass (Da):80,742
Last modified:August 1, 1998 - v1
Checksum:iEE6A6AD78D79174C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012759 mRNA. Translation: BAA25544.1.
RefSeqiNP_112614.1. NM_031324.1.
UniGeneiRn.11058.

Genome annotation databases

GeneIDi83471.
KEGGirno:83471.
UCSCiRGD:620841. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012759 mRNA. Translation: BAA25544.1.
RefSeqiNP_112614.1. NM_031324.1.
UniGeneiRn.11058.

3D structure databases

ProteinModelPortaliO70196.
SMRiO70196. Positions 1-709.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000360.

Chemistry

BindingDBiO70196.
ChEMBLiCHEMBL4035.

Protein family/group databases

MEROPSiS09.001.

PTM databases

PhosphoSiteiO70196.

Proteomic databases

PaxDbiO70196.
PRIDEiO70196.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi83471.
KEGGirno:83471.
UCSCiRGD:620841. rat.

Organism-specific databases

CTDi5550.
RGDi620841. Prep.

Phylogenomic databases

eggNOGiCOG1505.
HOGENOMiHOG000238967.
HOVERGENiHBG007251.
InParanoidiO70196.
KOiK01322.
PhylomeDBiO70196.

Miscellaneous databases

NextBioi615881.
PROiO70196.

Gene expression databases

GenevestigatoriO70196.

Family and domain databases

Gene3Di2.130.10.120. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002471. Pept_S9_AS.
IPR023302. Pept_S9A_N.
IPR001375. Peptidase_S9.
IPR002470. Peptidase_S9A.
[Graphical view]
PANTHERiPTHR11757. PTHR11757. 1 hit.
PfamiPF00326. Peptidase_S9. 1 hit.
PF02897. Peptidase_S9_N. 1 hit.
[Graphical view]
PRINTSiPR00862. PROLIGOPTASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00708. PRO_ENDOPEP_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "cDNA cloning of rat prolyl oligopeptidase and its expression in the ovary during the estrous cycle."
    Kimura A., Takahashi T.
    J. Exp. Zool. 286:656-665(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: Wistar1 Publication.
    Tissue: Liver1 Publication.
  2. Maurya D.K., Bhargava P.
    Submitted (JAN-2009) to UniProtKB
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiPPCE_RAT
AccessioniPrimary (citable) accession number: O70196
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: August 1, 1998
Last modified: January 7, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.