O70176 (PACA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pituitary adenylate cyclase-activating polypeptide
Short name=PACAP

Cleaved into the following 3 chains:

1. PACAP-related peptide
   Alternative name(s):
   PRP-48
2. Pituitary adenylate cyclase-activating polypeptide 27
   Short name=PACAP-27
   Short name=PACAP27
3. Pituitary adenylate cyclase-activating polypeptide 38
   Short name=PACAP-38
   Short name=PACAP38

Gene names

Name:	Adcyap1
Synonyms:	Pacap

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	175 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells By similarity.
Subunit structure	Interacts with ADCYAP1R1 (via N-terminal extracellular domain) By similarity.
Subcellular location	Secreted.
Sequence similarities	Belongs to the glucagon family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hormone
PTM	Amidation Cleavage on pair of basic residues
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ATP metabolic process Inferred from electronic annotation. Source: Compara activation of adenylate cyclase activity Traceable author statement PubMed 9729410. Source: MGI behavioral fear response Inferred from electronic annotation. Source: Compara cellular response to glucocorticoid stimulus Inferred from electronic annotation. Source: Compara elevation of cytosolic calcium ion concentration Inferred from electronic annotation. Source: Compara histamine secretion Inferred from electronic annotation. Source: Compara negative regulation of Rho GTPase activity Inferred from electronic annotation. Source: Compara negative regulation of acute inflammatory response to antigenic stimulus Inferred from electronic annotation. Source: Compara negative regulation of acute inflammatory response to non-antigenic stimulus Inferred from electronic annotation. Source: Compara negative regulation of cell cycle Inferred from direct assay PubMed 12417650. Source: MGI negative regulation of glial cell proliferation Inferred from electronic annotation. Source: Compara negative regulation of potassium ion transport Inferred from electronic annotation. Source: Compara neuropeptide signaling pathway Traceable author statement PubMed 9729410. Source: MGI ovarian follicle development Inferred from electronic annotation. Source: Compara pituitary gland development Inferred from electronic annotation. Source: Compara positive regulation of adenylate cyclase activity involved in G-protein coupled receptor signaling pathway Inferred from direct assay PubMed 9603988. Source: BHF-UCL positive regulation of cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of growth hormone secretion Inferred from electronic annotation. Source: Compara positive regulation of interleukin-6 production Inferred from electronic annotation. Source: Compara positive regulation of neuron projection development Inferred from electronic annotation. Source: Compara positive regulation of somatostatin secretion Inferred from electronic annotation. Source: Compara positive regulation of synaptic transmission, glutamatergic Inferred from electronic annotation. Source: Compara positive regulation of vasodilation Inferred from electronic annotation. Source: Compara regulation of G-protein coupled receptor protein signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB regulation of oligodendrocyte progenitor proliferation Inferred from electronic annotation. Source: Compara regulation of postsynaptic membrane potential Inferred from electronic annotation. Source: Compara regulation of protein localization Inferred from direct assay PubMed 9603988. Source: BHF-UCL response to ethanol Inferred from electronic annotation. Source: Compara response to starvation Inferred from electronic annotation. Source: Compara sensory perception of pain Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular space Inferred from electronic annotation. Source: Compara terminal bouton Inferred from electronic annotation. Source: Compara
Molecular_function	neuropeptide hormone activity Traceable author statement PubMed 9729410. Source: MGI peptide hormone receptor binding Inferred from sequence or structural similarity. Source: UniProtKB pituitary adenylate cyclase activating polypeptide activity Inferred from direct assay PubMed 9603988. Source: BHF-UCL receptor signaling protein activity Inferred from direct assay PubMed 12473665. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Propeptide	25 – 78	54			PRO_0000011491
	Peptide	81 – 128	48	PACAP-related peptide		PRO_0000011492
	Peptide	131 – 168	38	Pituitary adenylate cyclase-activating polypeptide 38		PRO_0000011493
	Peptide	131 – 157	27	Pituitary adenylate cyclase-activating polypeptide 27		PRO_0000011494
	Propeptide	172 – 175	4			PRO_0000011495
Regions
	Region	149 – 157	9	Important for receptor binding By similarity
Amino acid modifications
	Modified residue	157	1	Leucine amide By similarity
	Modified residue	168	1	Lysine amide By similarity

Sequences

	Sequence		Length	Mass (Da)
	O70176 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: D0E2007DB0C6E8C2	FASTA	175	19,381
	10 20 30 40 50 60 MTMCSGARLA LLVYGIIMHS SVSCSPAAGL SFPGIRPEDE AYDQDGNPLQ DFYDWDPPGV 70 80 90 100 110 120 GSPASALRDA YALYYPADRR DVAHEILNEA YRKVLDQLSA RKYLQSVVAR GAGENLGGSA 130 140 150 160 170 VDDPAPLTKR HSDGIFTDSY SRYRKQMAVK KYLAAVLGKR YKQRVKNKGR RIAYL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the mouse pituitary adenylate cyclase-activating polypeptide (PACAP) gene." Yamamoto K., Hashimoto H., Hagihara N., Nishino A., Fujita T., Matsuda T., Baba A. Gene 211:63-69(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB010149 Genomic DNA. Translation: BAA28355.1. BC057344 mRNA. Translation: AAH57344.1.
IPI	IPI00115715.
RefSeq	NP_033755.1. NM_009625.2.
UniGene	Mm.3407.
3D structure databases
ProteinModelPortal	O70176.
SMR	O70176. Positions 131-168.
ModBase	Search...
PTM databases
PhosphoSite	O70176.
Proteomic databases
PRIDE	O70176.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000064775; ENSMUSP00000067057; ENSMUSG00000024256.
GeneID	11516.
KEGG	mmu:11516.
Organism-specific databases
CTD	116.
MGI	MGI:105094. Adcyap1.
Phylogenomic databases
eggNOG	NOG39235.
HOGENOM	HOG000253943.
HOVERGEN	HBG018069.
InParanoid	O70176.
KO	K05262.
OMA	YCSPAAG.
OrthoDB	EOG4Q58QM.
Gene expression databases
ArrayExpress	O70176.
Bgee	O70176.
CleanEx	MM_ADCYAP1.
Genevestigator	O70176.
GermOnline	ENSMUSG00000024256. Mus musculus.
Family and domain databases
InterPro	IPR000532. Glucagon_GIP_secretin_VIP. [Graphical view]
Pfam	PF00123. Hormone_2. 2 hits. [Graphical view]
PRINTS	PR00275. GLUCAGON.
SMART	SM00070. GLUCA. 2 hits. [Graphical view]
PROSITE	PS00260. GLUCAGON. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	278934.
SOURCE	Search...

Entry information

Entry name

PACA_MOUSE

Accession

Primary (citable) accession number: O70176

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	August 1, 1998
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program