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O70176 (PACA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pituitary adenylate cyclase-activating polypeptide

Short name=PACAP

Cleaved into the following 3 chains:

  1. PACAP-related peptide
    Alternative name(s):
    PRP-48
  2. Pituitary adenylate cyclase-activating polypeptide 27
    Short name=PACAP-27
    Short name=PACAP27
  3. Pituitary adenylate cyclase-activating polypeptide 38
    Short name=PACAP-38
    Short name=PACAP38
Gene names
Name:Adcyap1
Synonyms:Pacap
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length175 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Promotes neuron projection development through the RAPGEF2/Rap1/B-Raf/ERK pathway By similarity.

Subunit structure

Interacts with ADCYAP1R1 (via N-terminal extracellular domain) By similarity.

Subcellular location

Secreted.

Sequence similarities

Belongs to the glucagon family.

Ontologies

Keywords
   Biological processNeurogenesis
   Cellular componentSecreted
   DomainSignal
   Molecular functionHormone
   PTMAmidation
Cleavage on pair of basic residues
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processATP metabolic process

Inferred from electronic annotation. Source: Ensembl

activation of adenylate cyclase activity

Traceable author statement PubMed 9729410. Source: MGI

behavioral fear response

Inferred from electronic annotation. Source: Ensembl

cAMP-mediated signaling

Inferred from sequence or structural similarity. Source: UniProtKB

cellular response to glucocorticoid stimulus

Inferred from electronic annotation. Source: Ensembl

histamine secretion

Inferred from electronic annotation. Source: Ensembl

negative regulation of Rho GTPase activity

Inferred from electronic annotation. Source: Ensembl

negative regulation of acute inflammatory response to antigenic stimulus

Inferred from electronic annotation. Source: Ensembl

negative regulation of acute inflammatory response to non-antigenic stimulus

Inferred from electronic annotation. Source: Ensembl

negative regulation of cell cycle

Inferred from direct assay PubMed 12417650. Source: MGI

negative regulation of glial cell proliferation

Inferred from electronic annotation. Source: Ensembl

negative regulation of muscle cell apoptotic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of potassium ion transport

Inferred from electronic annotation. Source: Ensembl

neuron projection development

Inferred from sequence or structural similarity. Source: UniProtKB

neuropeptide signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

ovarian follicle development

Inferred from electronic annotation. Source: Ensembl

pituitary gland development

Inferred from electronic annotation. Source: Ensembl

positive regulation of ERK1 and ERK2 cascade

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of Rap GTPase activity

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of adenylate cyclase activity involved in G-protein coupled receptor signaling pathway

Inferred from direct assay PubMed 9603988. Source: BHF-UCL

positive regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of cytosolic calcium ion concentration

Inferred from electronic annotation. Source: Ensembl

positive regulation of gene expression

Inferred from direct assay PubMed 23913443. Source: MGI

positive regulation of growth hormone secretion

Inferred from electronic annotation. Source: Ensembl

positive regulation of interleukin-6 production

Inferred from electronic annotation. Source: Ensembl

positive regulation of neuron projection development

Inferred from electronic annotation. Source: Ensembl

positive regulation of protein kinase activity

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of somatostatin secretion

Inferred from electronic annotation. Source: Ensembl

positive regulation of synaptic transmission, glutamatergic

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of vasodilation

Inferred from electronic annotation. Source: Ensembl

regulation of G-protein coupled receptor protein signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of oligodendrocyte progenitor proliferation

Inferred from electronic annotation. Source: Ensembl

regulation of postsynaptic membrane potential

Inferred from electronic annotation. Source: Ensembl

regulation of protein localization

Inferred from direct assay PubMed 9603988. Source: BHF-UCL

response to ethanol

Inferred from electronic annotation. Source: Ensembl

response to starvation

Inferred from electronic annotation. Source: Ensembl

sensory perception of pain

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentextracellular space

Inferred from electronic annotation. Source: Ensembl

terminal bouton

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionneuropeptide hormone activity

Inferred from sequence or structural similarity. Source: UniProtKB

peptide hormone receptor binding

Inferred from sequence or structural similarity. Source: UniProtKB

pituitary adenylate cyclase activating polypeptide activity

Inferred from direct assay PubMed 9603988. Source: BHF-UCL

receptor signaling protein activity

Inferred from direct assay PubMed 12473665. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Propeptide25 – 7854
PRO_0000011491
Peptide81 – 12848PACAP-related peptide
PRO_0000011492
Peptide131 – 16838Pituitary adenylate cyclase-activating polypeptide 38
PRO_0000011493
Peptide131 – 15727Pituitary adenylate cyclase-activating polypeptide 27
PRO_0000011494
Propeptide172 – 1754
PRO_0000011495

Regions

Region149 – 1579Important for receptor binding By similarity

Amino acid modifications

Modified residue1571Leucine amide By similarity
Modified residue1681Lysine amide By similarity

Sequences

Sequence LengthMass (Da)Tools
O70176 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: D0E2007DB0C6E8C2

FASTA17519,381
        10         20         30         40         50         60 
MTMCSGARLA LLVYGIIMHS SVSCSPAAGL SFPGIRPEDE AYDQDGNPLQ DFYDWDPPGV 

        70         80         90        100        110        120 
GSPASALRDA YALYYPADRR DVAHEILNEA YRKVLDQLSA RKYLQSVVAR GAGENLGGSA 

       130        140        150        160        170 
VDDPAPLTKR HSDGIFTDSY SRYRKQMAVK KYLAAVLGKR YKQRVKNKGR RIAYL 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of the mouse pituitary adenylate cyclase-activating polypeptide (PACAP) gene."
Yamamoto K., Hashimoto H., Hagihara N., Nishino A., Fujita T., Matsuda T., Baba A.
Gene 211:63-69(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/SvJ.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB010149 Genomic DNA. Translation: BAA28355.1.
BC057344 mRNA. Translation: AAH57344.1.
CCDSCCDS29029.1.
RefSeqNP_033755.1. NM_009625.2.
UniGeneMm.3407.

3D structure databases

ProteinModelPortalO70176.
SMRO70176. Positions 131-168.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid197979. 1 interaction.
MINTMINT-1512367.

PTM databases

PhosphoSiteO70176.

Proteomic databases

PRIDEO70176.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000064775; ENSMUSP00000067057; ENSMUSG00000024256.
GeneID11516.
KEGGmmu:11516.
UCSCuc008dwi.1. mouse.

Organism-specific databases

CTD116.
MGIMGI:105094. Adcyap1.

Phylogenomic databases

eggNOGNOG39235.
HOGENOMHOG000253943.
HOVERGENHBG018069.
InParanoidO70176.
KOK05262.
OMAPPEKRHA.
OrthoDBEOG7M6D8P.
PhylomeDBO70176.
TreeFamTF332804.

Gene expression databases

ArrayExpressO70176.
BgeeO70176.
CleanExMM_ADCYAP1.
GenevestigatorO70176.

Family and domain databases

InterProIPR000532. Glucagon_GIP_secretin_VIP.
[Graphical view]
PfamPF00123. Hormone_2. 2 hits.
[Graphical view]
PRINTSPR00275. GLUCAGON.
SMARTSM00070. GLUCA. 2 hits.
[Graphical view]
PROSITEPS00260. GLUCAGON. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio278934.
PROO70176.
SOURCESearch...

Entry information

Entry namePACA_MOUSE
AccessionPrimary (citable) accession number: O70176
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: August 1, 1998
Last modified: July 9, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot