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Protein

Neuronal acetylcholine receptor subunit alpha-4

Gene

Chrna4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodium ions.

GO - Molecular functioni

  • acetylcholine-activated cation-selective channel activity Source: MGI
  • acetylcholine receptor activity Source: MGI
  • ion channel activity Source: MGI

GO - Biological processi

  • acetylcholine receptor signaling pathway Source: MGI
  • action potential Source: MGI
  • B cell activation Source: MGI
  • behavioral response to nicotine Source: MGI
  • calcium ion transport Source: MGI
  • cognition Source: MGI
  • DNA repair Source: MGI
  • exploration behavior Source: MGI
  • inhibitory postsynaptic potential Source: MGI
  • locomotory behavior Source: MGI
  • membrane depolarization Source: MGI
  • neurological system process Source: MGI
  • neuromuscular synaptic transmission Source: GO_Central
  • regulation of dopamine secretion Source: MGI
  • regulation of membrane potential Source: MGI
  • respiratory gaseous exchange Source: MGI
  • response to hypoxia Source: MGI
  • response to nicotine Source: MGI
  • response to oxidative stress Source: MGI
  • sensory perception of pain Source: MGI
  • signal transduction Source: MGI
  • synaptic transmission, cholinergic Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-629587. Highly sodium permeable acetylcholine nicotinic receptors.
R-MMU-629594. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
R-MMU-629597. Highly calcium permeable nicotinic acetylcholine receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit alpha-4
Gene namesi
Name:Chrna4
Synonyms:Acra4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:87888. Chrna4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini32 – 249ExtracellularSequence analysisAdd BLAST218
Transmembranei250 – 270HelicalSequence analysisAdd BLAST21
Transmembranei279 – 299HelicalSequence analysisAdd BLAST21
Transmembranei312 – 332HelicalSequence analysisAdd BLAST21
Topological domaini333 – 603CytoplasmicSequence analysisAdd BLAST271
Transmembranei604 – 624HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • acetylcholine-gated channel complex Source: MGI
  • cell junction Source: UniProtKB-KW
  • external side of plasma membrane Source: MGI
  • membrane Source: MGI
  • plasma membrane Source: MGI
  • postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4457.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Sequence analysisAdd BLAST30
ChainiPRO_000000035231 – 629Neuronal acetylcholine receptor subunit alpha-4Add BLAST599

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi59N-linked (GlcNAc...)Sequence analysis1
Glycosylationi109N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi163 ↔ 177By similarity
Glycosylationi176N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi227 ↔ 228Associated with receptor activationBy similarity
Lipidationi273S-palmitoyl cysteineBy similarity1
Modified residuei427PhosphoserineBy similarity1
Modified residuei540PhosphoserineCombined sources1
Modified residuei543PhosphoserineCombined sources1

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiO70174.
PRIDEiO70174.

PTM databases

iPTMnetiO70174.
PhosphoSitePlusiO70174.

Expressioni

Gene expression databases

BgeeiENSMUSG00000027577.
ExpressionAtlasiO70174. baseline and differential.
GenevisibleiO70174. MM.

Interactioni

Subunit structurei

Neuronal AChR is composed of two different types of subunits: alpha and beta. Alpha-4 subunit can be combined to beta-2 or beta-4 to give rise to functional receptors, complexes with beta-2 may be heteropentamers. Interacts with RIC3; which is required for proper folding and assembly (By similarity).By similarity

Protein-protein interaction databases

BioGridi197932. 2 interactors.
DIPiDIP-48731N.
STRINGi10090.ENSMUSP00000066338.

Structurei

3D structure databases

ProteinModelPortaliO70174.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
GeneTreeiENSGT00760000118930.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiO70174.
KOiK04806.
PhylomeDBiO70174.
TreeFamiTF315605.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 4 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 2 hits.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O70174-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIGGSGAPP PLLLLPLLLL LGTGLLPASS HIETRAHAEE RLLKRLFSGY
60 70 80 90 100
NKWSRPVANI SDVVLVRFGL SIAQLIDVDE KNQMMTTNVW VKQEWHDYKL
110 120 130 140 150
RWDPGDYENV TSIRIPSELI WRPDIVLYNN ADGDFAVTHL TKAHLFYDGR
160 170 180 190 200
VQWTPPAIYK SSCSIDVTFF PFDQQNCTMK FGSWTYDKAK IDLVSMHSRV
210 220 230 240 250
DQLDFWESGE WVIVDAVGTY NTRKYECCAE IYPDITYAFI IRRLPLFYTI
260 270 280 290 300
NLIIPCLLIS CLTVLVFYLP SECGEKVTLC ISVLLSLTVF LLLITEIIPS
310 320 330 340 350
TSLVIPLIGE YLLFTMIFVT LSIVITVFVL NVHHRSPRTH TMPAWVRRVF
360 370 380 390 400
LDIVPRLLFM KRPSVVKDNC RRLIESMHKM ANAPRFWPEP ESEPGILGDI
410 420 430 440 450
CNQGLSPAPT FCNRMDTAVE TQPTCRSPSH KVPDLKTSEV EKASPCPSPG
460 470 480 490 500
SCHPPNSSGA PVLIKARSLS VQHVPSSQEA AEGSIRCRSR SIQYCVSQDG
510 520 530 540 550
AASLTESKPT GSPASLKTRP SQLPVSDQTS PCKCTCKEPS PVSPITVLKA
560 570 580 590 600
GGTKAPPQHL PLSPALTRAV EGVQYIADHL KAEDTDFSVK EDWKYVAMVI
610 620
DRIFLWMFII VCLLGTVGLF LPPWLAGMI
Length:629
Mass (Da):70,305
Last modified:September 19, 2003 - v2
Checksum:i261455B6ED50B41C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16P → L in AAF34716 (PubMed:11434511).Curated1
Sequence conflicti24G → A in BAA25752 (PubMed:9749753).Curated1
Sequence conflicti134D → N in AAF34716 (PubMed:11434511).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti529T → A.1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF225912 mRNA. Translation: AAF34716.2.
AK034228 mRNA. Translation: BAC28638.1.
AK083157 mRNA. Translation: BAC38788.1.
BC053013 mRNA. Translation: AAH53013.1.
AB010002 Genomic DNA. Translation: BAA25752.1.
AF325347 mRNA. Translation: AAL37363.1.
CCDSiCCDS17192.1.
RefSeqiNP_056545.3. NM_015730.5.
UniGeneiMm.252369.

Genome annotation databases

EnsembliENSMUST00000067120; ENSMUSP00000066338; ENSMUSG00000027577.
GeneIDi11438.
KEGGimmu:11438.
UCSCiuc008okq.1. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF225912 mRNA. Translation: AAF34716.2.
AK034228 mRNA. Translation: BAC28638.1.
AK083157 mRNA. Translation: BAC38788.1.
BC053013 mRNA. Translation: AAH53013.1.
AB010002 Genomic DNA. Translation: BAA25752.1.
AF325347 mRNA. Translation: AAL37363.1.
CCDSiCCDS17192.1.
RefSeqiNP_056545.3. NM_015730.5.
UniGeneiMm.252369.

3D structure databases

ProteinModelPortaliO70174.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi197932. 2 interactors.
DIPiDIP-48731N.
STRINGi10090.ENSMUSP00000066338.

Chemistry databases

ChEMBLiCHEMBL4457.

PTM databases

iPTMnetiO70174.
PhosphoSitePlusiO70174.

Proteomic databases

PaxDbiO70174.
PRIDEiO70174.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000067120; ENSMUSP00000066338; ENSMUSG00000027577.
GeneIDi11438.
KEGGimmu:11438.
UCSCiuc008okq.1. mouse.

Organism-specific databases

CTDi1137.
MGIiMGI:87888. Chrna4.

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
GeneTreeiENSGT00760000118930.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiO70174.
KOiK04806.
PhylomeDBiO70174.
TreeFamiTF315605.

Enzyme and pathway databases

ReactomeiR-MMU-629587. Highly sodium permeable acetylcholine nicotinic receptors.
R-MMU-629594. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
R-MMU-629597. Highly calcium permeable nicotinic acetylcholine receptors.

Miscellaneous databases

PROiO70174.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027577.
ExpressionAtlasiO70174. baseline and differential.
GenevisibleiO70174. MM.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 4 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 2 hits.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACHA4_MOUSE
AccessioniPrimary (citable) accession number: O70174
Secondary accession number(s): Q8BHE9, Q8VI10, Q9ET51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: September 19, 2003
Last modified: November 2, 2016
This is version 148 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.