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Protein

Neuronal acetylcholine receptor subunit alpha-4

Gene

Chrna4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodium ions.

GO - Molecular functioni

  • acetylcholine-activated cation-selective channel activity Source: MGI
  • acetylcholine receptor activity Source: MGI
  • ion channel activity Source: MGI

GO - Biological processi

  • action potential Source: MGI
  • B cell activation Source: MGI
  • behavioral response to nicotine Source: MGI
  • calcium ion transport Source: MGI
  • cation transmembrane transport Source: MGI
  • cognition Source: MGI
  • DNA repair Source: MGI
  • exploration behavior Source: MGI
  • locomotory behavior Source: MGI
  • membrane depolarization Source: MGI
  • neurological system process Source: MGI
  • regulation of dopamine secretion Source: MGI
  • regulation of inhibitory postsynaptic membrane potential Source: MGI
  • regulation of membrane potential Source: MGI
  • respiratory gaseous exchange Source: MGI
  • response to hypoxia Source: MGI
  • response to nicotine Source: MGI
  • response to oxidative stress Source: MGI
  • sensory perception of pain Source: MGI
  • signal transduction Source: MGI
  • synaptic transmission, cholinergic Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

ReactomeiREACT_291046. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
REACT_305614. Highly sodium permeable acetylcholine nicotinic receptors.
REACT_329749. Highly calcium permeable nicotinic acetylcholine receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit alpha-4
Gene namesi
Name:Chrna4
Synonyms:Acra4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:87888. Chrna4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini32 – 249218ExtracellularSequence AnalysisAdd
BLAST
Transmembranei250 – 27021HelicalSequence AnalysisAdd
BLAST
Transmembranei279 – 29921HelicalSequence AnalysisAdd
BLAST
Transmembranei312 – 33221HelicalSequence AnalysisAdd
BLAST
Topological domaini333 – 603271CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei604 – 62421HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • acetylcholine-gated channel complex Source: MGI
  • cell junction Source: UniProtKB-KW
  • external side of plasma membrane Source: MGI
  • membrane Source: MGI
  • plasma membrane Source: MGI
  • postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Sequence AnalysisAdd
BLAST
Chaini31 – 629599Neuronal acetylcholine receptor subunit alpha-4PRO_0000000352Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi59 – 591N-linked (GlcNAc...)Sequence Analysis
Glycosylationi109 – 1091N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi163 ↔ 177By similarity
Glycosylationi176 – 1761N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi227 ↔ 228Associated with receptor activationBy similarity
Lipidationi273 – 2731S-palmitoyl cysteineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

MaxQBiO70174.
PaxDbiO70174.
PRIDEiO70174.

PTM databases

PhosphoSiteiO70174.

Expressioni

Gene expression databases

BgeeiO70174.
ExpressionAtlasiO70174. baseline and differential.
GenevestigatoriO70174.

Interactioni

Subunit structurei

Neuronal AChR is composed of two different types of subunits: alpha and beta. Alpha-4 subunit can be combined to beta-2 or beta-4 to give rise to functional receptors, complexes with beta-2 may be heteropentamers. Interacts with RIC3; which is required for proper folding and assembly (By similarity).By similarity

Protein-protein interaction databases

BioGridi197932. 2 interactions.
DIPiDIP-48731N.

Structurei

3D structure databases

ProteinModelPortaliO70174.
SMRiO70174. Positions 39-387, 600-627.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG290206.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiO70174.
KOiK04806.
PhylomeDBiO70174.
TreeFamiTF315605.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 2 hits.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O70174-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIGGSGAPP PLLLLPLLLL LGTGLLPASS HIETRAHAEE RLLKRLFSGY
60 70 80 90 100
NKWSRPVANI SDVVLVRFGL SIAQLIDVDE KNQMMTTNVW VKQEWHDYKL
110 120 130 140 150
RWDPGDYENV TSIRIPSELI WRPDIVLYNN ADGDFAVTHL TKAHLFYDGR
160 170 180 190 200
VQWTPPAIYK SSCSIDVTFF PFDQQNCTMK FGSWTYDKAK IDLVSMHSRV
210 220 230 240 250
DQLDFWESGE WVIVDAVGTY NTRKYECCAE IYPDITYAFI IRRLPLFYTI
260 270 280 290 300
NLIIPCLLIS CLTVLVFYLP SECGEKVTLC ISVLLSLTVF LLLITEIIPS
310 320 330 340 350
TSLVIPLIGE YLLFTMIFVT LSIVITVFVL NVHHRSPRTH TMPAWVRRVF
360 370 380 390 400
LDIVPRLLFM KRPSVVKDNC RRLIESMHKM ANAPRFWPEP ESEPGILGDI
410 420 430 440 450
CNQGLSPAPT FCNRMDTAVE TQPTCRSPSH KVPDLKTSEV EKASPCPSPG
460 470 480 490 500
SCHPPNSSGA PVLIKARSLS VQHVPSSQEA AEGSIRCRSR SIQYCVSQDG
510 520 530 540 550
AASLTESKPT GSPASLKTRP SQLPVSDQTS PCKCTCKEPS PVSPITVLKA
560 570 580 590 600
GGTKAPPQHL PLSPALTRAV EGVQYIADHL KAEDTDFSVK EDWKYVAMVI
610 620
DRIFLWMFII VCLLGTVGLF LPPWLAGMI
Length:629
Mass (Da):70,305
Last modified:September 19, 2003 - v2
Checksum:i261455B6ED50B41C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti16 – 161P → L in AAF34716 (PubMed:11434511).Curated
Sequence conflicti24 – 241G → A in BAA25752 (PubMed:9749753).Curated
Sequence conflicti134 – 1341D → N in AAF34716 (PubMed:11434511).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti529 – 5291T → A.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF225912 mRNA. Translation: AAF34716.2.
AK034228 mRNA. Translation: BAC28638.1.
AK083157 mRNA. Translation: BAC38788.1.
BC053013 mRNA. Translation: AAH53013.1.
AB010002 Genomic DNA. Translation: BAA25752.1.
AF325347 mRNA. Translation: AAL37363.1.
CCDSiCCDS17192.1.
RefSeqiNP_056545.3. NM_015730.5.
UniGeneiMm.252369.

Genome annotation databases

EnsembliENSMUST00000067120; ENSMUSP00000066338; ENSMUSG00000027577.
GeneIDi11438.
KEGGimmu:11438.
UCSCiuc008okq.1. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF225912 mRNA. Translation: AAF34716.2.
AK034228 mRNA. Translation: BAC28638.1.
AK083157 mRNA. Translation: BAC38788.1.
BC053013 mRNA. Translation: AAH53013.1.
AB010002 Genomic DNA. Translation: BAA25752.1.
AF325347 mRNA. Translation: AAL37363.1.
CCDSiCCDS17192.1.
RefSeqiNP_056545.3. NM_015730.5.
UniGeneiMm.252369.

3D structure databases

ProteinModelPortaliO70174.
SMRiO70174. Positions 39-387, 600-627.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi197932. 2 interactions.
DIPiDIP-48731N.

Chemistry

ChEMBLiCHEMBL4457.
GuidetoPHARMACOLOGYi465.

PTM databases

PhosphoSiteiO70174.

Proteomic databases

MaxQBiO70174.
PaxDbiO70174.
PRIDEiO70174.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000067120; ENSMUSP00000066338; ENSMUSG00000027577.
GeneIDi11438.
KEGGimmu:11438.
UCSCiuc008okq.1. mouse.

Organism-specific databases

CTDi1137.
MGIiMGI:87888. Chrna4.

Phylogenomic databases

eggNOGiNOG290206.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiO70174.
KOiK04806.
PhylomeDBiO70174.
TreeFamiTF315605.

Enzyme and pathway databases

ReactomeiREACT_291046. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
REACT_305614. Highly sodium permeable acetylcholine nicotinic receptors.
REACT_329749. Highly calcium permeable nicotinic acetylcholine receptors.

Miscellaneous databases

NextBioi278736.
PROiO70174.
SOURCEiSearch...

Gene expression databases

BgeeiO70174.
ExpressionAtlasiO70174. baseline and differential.
GenevestigatoriO70174.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 2 hits.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Long sleep and short sleep mice differ in nicotine-stimulated 86Rb+ efflux and alpha4 nicotinic receptor subunit cDNA sequence."
    Stitzel J.A., Dobelis P., Jimenez M., Collins A.C.
    Pharmacogenetics 11:331-339(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ALA-529.
    Strain: Long sleep selected line.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Diencephalon and Hippocampus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  4. "Promoter analysis of the neuronal nicotinic acetylcholine receptor alpha4 gene: methylation and expression of the transgene."
    Watanabe H., Zoli M., Changeux J.-P.
    Eur. J. Neurosci. 10:2244-2253(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-27.
  5. "Expression of mouse nicotinic acetylcholine receptor genes in the developing thymus."
    Kuo Y.-P., Lukas R.J.
    Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 118-357.
    Strain: C57BL/6.

Entry informationi

Entry nameiACHA4_MOUSE
AccessioniPrimary (citable) accession number: O70174
Secondary accession number(s): Q8BHE9, Q8VI10, Q9ET51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: September 19, 2003
Last modified: April 1, 2015
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.