O70174 (ACHA4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neuronal acetylcholine receptor subunit alpha-4

Gene names

Name:	Chrna4
Synonyms:	Acra4

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	629 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodium ions.
Subunit structure	Neuronal AChR is composed of two different types of subunits: alpha and beta. Alpha-4 subunit can be combined to beta-2 or beta-4 to give rise to functional receptors, complexes with beta-2 may be heteropentamers. Interacts with RIC3; which is required for proper folding and assembly By similarity.
Subcellular location	Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Cell membrane; Lipid-anchor By similarity.
Sequence similarities	Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-4/CHRNA4 sub-subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Coding sequence diversity	Polymorphism
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Ion channel Ligand-gated ion channel Receptor
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	B cell activation Inferred from mutant phenotype PubMed 15963492. Source: MGI DNA repair Inferred from electronic annotation. Source: Compara behavioral response to nicotine Inferred from mutant phenotype PubMed 15528443. Source: MGI calcium ion transport Inferred from genetic interaction PubMed 15569257. Source: MGI cognition Inferred from electronic annotation. Source: Compara locomotory behavior Inferred from mutant phenotype PubMed 11226318. Source: MGI membrane depolarization Inferred from mutant phenotype PubMed 11222635PubMed 11226318PubMed 12944511. Source: MGI regulation of action potential Inferred from mutant phenotype PubMed 10235262. Source: MGI regulation of dopamine secretion Inferred from mutant phenotype PubMed 12944511. Source: MGI regulation of inhibitory postsynaptic membrane potential Inferred from mutant phenotype PubMed 17146052. Source: MGI respiratory gaseous exchange Inferred from mutant phenotype PubMed 21606356. Source: MGI response to hypoxia Inferred from electronic annotation. Source: Compara response to oxidative stress Inferred from electronic annotation. Source: Compara sensory perception of pain Inferred from mutant phenotype PubMed 10235262. Source: MGI synaptic transmission, cholinergic Inferred from direct assay PubMed 11906696. Source: MGI
Cellular_component	acetylcholine-gated channel complex Inferred from direct assay PubMed 12944511. Source: MGI cell junction Inferred from electronic annotation. Source: UniProtKB-KW external side of plasma membrane Inferred from direct assay PubMed 15963492. Source: MGI postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	acetylcholine receptor activity Inferred from electronic annotation. Source: Compara acetylcholine-activated cation-selective channel activity Inferred from mutant phenotype PubMed 11222635PubMed 12130686PubMed 15528443. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Potential
	Chain	31 – 629	599	Neuronal acetylcholine receptor subunit alpha-4		PRO_0000000352
Regions
	Topological domain	32 – 249	218	Extracellular Potential
	Transmembrane	250 – 270	21	Helical; Potential
	Transmembrane	279 – 299	21	Helical; Potential
	Transmembrane	312 – 332	21	Helical; Potential
	Topological domain	333 – 603	271	Cytoplasmic Potential
	Transmembrane	604 – 624	21	Helical; Potential
Amino acid modifications
	Lipidation	273	1	S-palmitoyl cysteine By similarity
	Glycosylation	59	1	N-linked (GlcNAc...) Potential
	Glycosylation	109	1	N-linked (GlcNAc...) Potential
	Glycosylation	176	1	N-linked (GlcNAc...) Potential
	Disulfide bond	163 ↔ 177		By similarity
	Disulfide bond	227 ↔ 228		Associated with receptor activation By similarity
Natural variations
	Natural variant	529	1	T → A. Ref.1
Experimental info
	Sequence conflict	16	1	P → L in AAF34716. Ref.1
	Sequence conflict	24	1	G → A in BAA25752. Ref.4
	Sequence conflict	134	1	D → N in AAF34716. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O70174 [UniParc]. Last modified September 19, 2003. Version 2. Checksum: 261455B6ED50B41C	FASTA	629	70,305
	10 20 30 40 50 60 MEIGGSGAPP PLLLLPLLLL LGTGLLPASS HIETRAHAEE RLLKRLFSGY NKWSRPVANI 70 80 90 100 110 120 SDVVLVRFGL SIAQLIDVDE KNQMMTTNVW VKQEWHDYKL RWDPGDYENV TSIRIPSELI 130 140 150 160 170 180 WRPDIVLYNN ADGDFAVTHL TKAHLFYDGR VQWTPPAIYK SSCSIDVTFF PFDQQNCTMK 190 200 210 220 230 240 FGSWTYDKAK IDLVSMHSRV DQLDFWESGE WVIVDAVGTY NTRKYECCAE IYPDITYAFI 250 260 270 280 290 300 IRRLPLFYTI NLIIPCLLIS CLTVLVFYLP SECGEKVTLC ISVLLSLTVF LLLITEIIPS 310 320 330 340 350 360 TSLVIPLIGE YLLFTMIFVT LSIVITVFVL NVHHRSPRTH TMPAWVRRVF LDIVPRLLFM 370 380 390 400 410 420 KRPSVVKDNC RRLIESMHKM ANAPRFWPEP ESEPGILGDI CNQGLSPAPT FCNRMDTAVE 430 440 450 460 470 480 TQPTCRSPSH KVPDLKTSEV EKASPCPSPG SCHPPNSSGA PVLIKARSLS VQHVPSSQEA 490 500 510 520 530 540 AEGSIRCRSR SIQYCVSQDG AASLTESKPT GSPASLKTRP SQLPVSDQTS PCKCTCKEPS 550 560 570 580 590 600 PVSPITVLKA GGTKAPPQHL PLSPALTRAV EGVQYIADHL KAEDTDFSVK EDWKYVAMVI 610 620 DRIFLWMFII VCLLGTVGLF LPPWLAGMI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Long sleep and short sleep mice differ in nicotine-stimulated 86Rb+ efflux and alpha4 nicotinic receptor subunit cDNA sequence." Stitzel J.A., Dobelis P., Jimenez M., Collins A.C. Pharmacogenetics 11:331-339(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ALA-529. Strain: Long sleep selected line.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Diencephalon and Hippocampus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[4]	"Promoter analysis of the neuronal nicotinic acetylcholine receptor alpha4 gene: methylation and expression of the transgene." Watanabe H., Zoli M., Changeux J.-P. Eur. J. Neurosci. 10:2244-2253(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-27.
[5]	"Expression of mouse nicotinic acetylcholine receptor genes in the developing thymus." Kuo Y.-P., Lukas R.J. Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 118-357. Strain: C57BL/6.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF225912 mRNA. Translation: AAF34716.2. AK034228 mRNA. Translation: BAC28638.1. AK083157 mRNA. Translation: BAC38788.1. BC053013 mRNA. Translation: AAH53013.1. AB010002 Genomic DNA. Translation: BAA25752.1. AF325347 mRNA. Translation: AAL37363.1.
IPI	IPI00115712.
RefSeq	NP_056545.3. NM_015730.5.
UniGene	Mm.252369.
3D structure databases
ProteinModelPortal	O70174.
SMR	O70174. Positions 39-341, 600-627.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48731N.
PTM databases
PhosphoSite	O70174.
Proteomic databases
PaxDb	O70174.
PRIDE	O70174.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000067120; ENSMUSP00000066338; ENSMUSG00000027577.
GeneID	11438.
KEGG	mmu:11438.
Organism-specific databases
CTD	1137.
MGI	MGI:87888. Chrna4.
Phylogenomic databases
eggNOG	NOG290206.
HOGENOM	HOG000006756.
HOVERGEN	HBG003756.
KO	K04806.
OrthoDB	EOG480HWN.
Gene expression databases
ArrayExpress	O70174.
Bgee	O70174.
Genevestigator	O70174.
GermOnline	ENSMUSG00000027577. Mus musculus.
Family and domain databases
Gene3D	2.70.170.10. 1 hit.
InterPro	IPR006202. Neur_chan_lig-bd. IPR006201. Neur_channel. IPR006029. Neurotrans-gated_channel_TM. IPR018000. Neurotransmitter_ion_chnl_CS. IPR002394. Nicotinic_acetylcholine_rcpt. [Graphical view]
PANTHER	PTHR18945. PTHR18945. 1 hit.
Pfam	PF02931. Neur_chan_LBD. 1 hit. PF02932. Neur_chan_memb. 1 hit. [Graphical view]
PRINTS	PR00254. NICOTINICR. PR00252. NRIONCHANNEL.
SUPFAM	SSF90112. Neu_channel_TM. 1 hit. SSF63712. Neur_chan_LBD. 1 hit.
TIGRFAMs	TIGR00860. LIC. 1 hit.
PROSITE	PS00236. NEUROTR_ION_CHANNEL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	O70174.
ChEMBL	CHEMBL4457.
ChiTaRS	CHRNA4. mouse.
NextBio	278736.
SOURCE	Search...

Entry information

Entry name

ACHA4_MOUSE

Accession

Primary (citable) accession number: O70174
Secondary accession number(s): Q8BHE9, Q8VI10, Q9ET51

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	September 19, 2003
Last modified:	May 1, 2013
This is version 114 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program