Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O70174

- ACHA4_MOUSE

UniProt

O70174 - ACHA4_MOUSE

Protein

Neuronal acetylcholine receptor subunit alpha-4

Gene

Chrna4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 2 (19 Sep 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodium ions.

    GO - Molecular functioni

    1. acetylcholine-activated cation-selective channel activity Source: MGI
    2. acetylcholine receptor activity Source: Ensembl
    3. ion channel activity Source: MGI
    4. protein binding Source: MGI

    GO - Biological processi

    1. action potential Source: MGI
    2. B cell activation Source: MGI
    3. behavioral response to nicotine Source: MGI
    4. calcium ion transport Source: MGI
    5. cognition Source: Ensembl
    6. DNA repair Source: Ensembl
    7. exploration behavior Source: MGI
    8. ion transmembrane transport Source: GOC
    9. locomotory behavior Source: MGI
    10. membrane depolarization Source: MGI
    11. neurological system process Source: MGI
    12. regulation of dopamine secretion Source: MGI
    13. regulation of inhibitory postsynaptic membrane potential Source: MGI
    14. regulation of membrane potential Source: MGI
    15. respiratory gaseous exchange Source: MGI
    16. response to hypoxia Source: Ensembl
    17. response to nicotine Source: MGI
    18. response to oxidative stress Source: Ensembl
    19. sensory perception of pain Source: MGI
    20. synaptic transmission, cholinergic Source: MGI

    Keywords - Molecular functioni

    Ion channel, Ligand-gated ion channel, Receptor

    Keywords - Biological processi

    Ion transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_198583. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
    REACT_198585. Highly calcium permeable nicotinic acetylcholine receptors.
    REACT_198593. Highly sodium permeable acetylcholine nicotinic receptors.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neuronal acetylcholine receptor subunit alpha-4
    Gene namesi
    Name:Chrna4
    Synonyms:Acra4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:87888. Chrna4.

    Subcellular locationi

    GO - Cellular componenti

    1. acetylcholine-gated channel complex Source: MGI
    2. cell junction Source: UniProtKB-KW
    3. external side of plasma membrane Source: MGI
    4. membrane Source: MGI
    5. plasma membrane Source: MGI
    6. postsynaptic membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3030Sequence AnalysisAdd
    BLAST
    Chaini31 – 629599Neuronal acetylcholine receptor subunit alpha-4PRO_0000000352Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi59 – 591N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi109 – 1091N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi163 ↔ 177By similarity
    Glycosylationi176 – 1761N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi227 ↔ 228Associated with receptor activationBy similarity
    Lipidationi273 – 2731S-palmitoyl cysteineBy similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

    Proteomic databases

    PaxDbiO70174.
    PRIDEiO70174.

    PTM databases

    PhosphoSiteiO70174.

    Expressioni

    Gene expression databases

    BgeeiO70174.
    GenevestigatoriO70174.

    Interactioni

    Subunit structurei

    Neuronal AChR is composed of two different types of subunits: alpha and beta. Alpha-4 subunit can be combined to beta-2 or beta-4 to give rise to functional receptors, complexes with beta-2 may be heteropentamers. Interacts with RIC3; which is required for proper folding and assembly By similarity.By similarity

    Protein-protein interaction databases

    BioGridi197932. 2 interactions.
    DIPiDIP-48731N.

    Structurei

    3D structure databases

    ProteinModelPortaliO70174.
    SMRiO70174. Positions 39-341, 600-627.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini32 – 249218ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini333 – 603271CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei250 – 27021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei279 – 29921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei312 – 33221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei604 – 62421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG290206.
    HOGENOMiHOG000006756.
    HOVERGENiHBG003756.
    KOiK04806.
    PhylomeDBiO70174.
    TreeFamiTF315605.

    Family and domain databases

    Gene3Di1.20.120.370. 2 hits.
    2.70.170.10. 1 hit.
    InterProiIPR027361. Acetylcholine_rcpt_TM.
    IPR006202. Neur_chan_lig-bd.
    IPR006201. Neur_channel.
    IPR006029. Neurotrans-gated_channel_TM.
    IPR018000. Neurotransmitter_ion_chnl_CS.
    IPR002394. Nicotinic_acetylcholine_rcpt.
    [Graphical view]
    PANTHERiPTHR18945. PTHR18945. 1 hit.
    PfamiPF02931. Neur_chan_LBD. 1 hit.
    PF02932. Neur_chan_memb. 1 hit.
    [Graphical view]
    PRINTSiPR00254. NICOTINICR.
    PR00252. NRIONCHANNEL.
    SUPFAMiSSF63712. SSF63712. 1 hit.
    SSF90112. SSF90112. 2 hits.
    TIGRFAMsiTIGR00860. LIC. 1 hit.
    PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O70174-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEIGGSGAPP PLLLLPLLLL LGTGLLPASS HIETRAHAEE RLLKRLFSGY    50
    NKWSRPVANI SDVVLVRFGL SIAQLIDVDE KNQMMTTNVW VKQEWHDYKL 100
    RWDPGDYENV TSIRIPSELI WRPDIVLYNN ADGDFAVTHL TKAHLFYDGR 150
    VQWTPPAIYK SSCSIDVTFF PFDQQNCTMK FGSWTYDKAK IDLVSMHSRV 200
    DQLDFWESGE WVIVDAVGTY NTRKYECCAE IYPDITYAFI IRRLPLFYTI 250
    NLIIPCLLIS CLTVLVFYLP SECGEKVTLC ISVLLSLTVF LLLITEIIPS 300
    TSLVIPLIGE YLLFTMIFVT LSIVITVFVL NVHHRSPRTH TMPAWVRRVF 350
    LDIVPRLLFM KRPSVVKDNC RRLIESMHKM ANAPRFWPEP ESEPGILGDI 400
    CNQGLSPAPT FCNRMDTAVE TQPTCRSPSH KVPDLKTSEV EKASPCPSPG 450
    SCHPPNSSGA PVLIKARSLS VQHVPSSQEA AEGSIRCRSR SIQYCVSQDG 500
    AASLTESKPT GSPASLKTRP SQLPVSDQTS PCKCTCKEPS PVSPITVLKA 550
    GGTKAPPQHL PLSPALTRAV EGVQYIADHL KAEDTDFSVK EDWKYVAMVI 600
    DRIFLWMFII VCLLGTVGLF LPPWLAGMI 629
    Length:629
    Mass (Da):70,305
    Last modified:September 19, 2003 - v2
    Checksum:i261455B6ED50B41C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti16 – 161P → L in AAF34716. (PubMed:11434511)Curated
    Sequence conflicti24 – 241G → A in BAA25752. (PubMed:9749753)Curated
    Sequence conflicti134 – 1341D → N in AAF34716. (PubMed:11434511)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti529 – 5291T → A.1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF225912 mRNA. Translation: AAF34716.2.
    AK034228 mRNA. Translation: BAC28638.1.
    AK083157 mRNA. Translation: BAC38788.1.
    BC053013 mRNA. Translation: AAH53013.1.
    AB010002 Genomic DNA. Translation: BAA25752.1.
    AF325347 mRNA. Translation: AAL37363.1.
    CCDSiCCDS17192.1.
    RefSeqiNP_056545.3. NM_015730.5.
    UniGeneiMm.252369.

    Genome annotation databases

    EnsembliENSMUST00000067120; ENSMUSP00000066338; ENSMUSG00000027577.
    GeneIDi11438.
    KEGGimmu:11438.
    UCSCiuc008okq.1. mouse.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF225912 mRNA. Translation: AAF34716.2 .
    AK034228 mRNA. Translation: BAC28638.1 .
    AK083157 mRNA. Translation: BAC38788.1 .
    BC053013 mRNA. Translation: AAH53013.1 .
    AB010002 Genomic DNA. Translation: BAA25752.1 .
    AF325347 mRNA. Translation: AAL37363.1 .
    CCDSi CCDS17192.1.
    RefSeqi NP_056545.3. NM_015730.5.
    UniGenei Mm.252369.

    3D structure databases

    ProteinModelPortali O70174.
    SMRi O70174. Positions 39-341, 600-627.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 197932. 2 interactions.
    DIPi DIP-48731N.

    Chemistry

    BindingDBi O70174.
    GuidetoPHARMACOLOGYi 465.

    PTM databases

    PhosphoSitei O70174.

    Proteomic databases

    PaxDbi O70174.
    PRIDEi O70174.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000067120 ; ENSMUSP00000066338 ; ENSMUSG00000027577 .
    GeneIDi 11438.
    KEGGi mmu:11438.
    UCSCi uc008okq.1. mouse.

    Organism-specific databases

    CTDi 1137.
    MGIi MGI:87888. Chrna4.

    Phylogenomic databases

    eggNOGi NOG290206.
    HOGENOMi HOG000006756.
    HOVERGENi HBG003756.
    KOi K04806.
    PhylomeDBi O70174.
    TreeFami TF315605.

    Enzyme and pathway databases

    Reactomei REACT_198583. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
    REACT_198585. Highly calcium permeable nicotinic acetylcholine receptors.
    REACT_198593. Highly sodium permeable acetylcholine nicotinic receptors.

    Miscellaneous databases

    ChiTaRSi CHRNA4. mouse.
    NextBioi 278736.
    PROi O70174.
    SOURCEi Search...

    Gene expression databases

    Bgeei O70174.
    Genevestigatori O70174.

    Family and domain databases

    Gene3Di 1.20.120.370. 2 hits.
    2.70.170.10. 1 hit.
    InterProi IPR027361. Acetylcholine_rcpt_TM.
    IPR006202. Neur_chan_lig-bd.
    IPR006201. Neur_channel.
    IPR006029. Neurotrans-gated_channel_TM.
    IPR018000. Neurotransmitter_ion_chnl_CS.
    IPR002394. Nicotinic_acetylcholine_rcpt.
    [Graphical view ]
    PANTHERi PTHR18945. PTHR18945. 1 hit.
    Pfami PF02931. Neur_chan_LBD. 1 hit.
    PF02932. Neur_chan_memb. 1 hit.
    [Graphical view ]
    PRINTSi PR00254. NICOTINICR.
    PR00252. NRIONCHANNEL.
    SUPFAMi SSF63712. SSF63712. 1 hit.
    SSF90112. SSF90112. 2 hits.
    TIGRFAMsi TIGR00860. LIC. 1 hit.
    PROSITEi PS00236. NEUROTR_ION_CHANNEL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Long sleep and short sleep mice differ in nicotine-stimulated 86Rb+ efflux and alpha4 nicotinic receptor subunit cDNA sequence."
      Stitzel J.A., Dobelis P., Jimenez M., Collins A.C.
      Pharmacogenetics 11:331-339(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ALA-529.
      Strain: Long sleep selected line.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Diencephalon and Hippocampus.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    4. "Promoter analysis of the neuronal nicotinic acetylcholine receptor alpha4 gene: methylation and expression of the transgene."
      Watanabe H., Zoli M., Changeux J.-P.
      Eur. J. Neurosci. 10:2244-2253(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-27.
    5. "Expression of mouse nicotinic acetylcholine receptor genes in the developing thymus."
      Kuo Y.-P., Lukas R.J.
      Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 118-357.
      Strain: C57BL/6.

    Entry informationi

    Entry nameiACHA4_MOUSE
    AccessioniPrimary (citable) accession number: O70174
    Secondary accession number(s): Q8BHE9, Q8VI10, Q9ET51
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: September 19, 2003
    Last modified: October 1, 2014
    This is version 128 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3