Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha

Gene

Pip4k2a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of phosphatidylinositol 5-phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. May regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation. May negatively regulate insulin-stimulated glucose uptake by lowering the levels of PtdIns5P (By similarity). May be involved in thrombopoiesis and the terminal maturation of megakaryocytes and regulation of their size.By similarity1 Publication

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.

GO - Molecular functioni

GO - Biological processi

  • megakaryocyte development Source: UniProtKB
  • positive regulation of autophagosome assembly Source: MGI
  • regulation of autophagy Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1660499. Synthesis of PIPs at the plasma membrane.
R-MMU-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha (EC:2.7.1.149)
Alternative name(s):
1-phosphatidylinositol 5-phosphate 4-kinase 2-alpha
Diphosphoinositide kinase 2-alpha
Phosphatidylinositol 5-phosphate 4-kinase type II alpha
Short name:
PI(5)P 4-kinase type II alpha
Short name:
PIP4KII-alpha
PtdIns(5)P-4-kinase isoform 2-alpha
Gene namesi
Name:Pip4k2a
Synonyms:Pip5k2a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1298206. Pip4k2a.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm
  • Cell membrane

  • Note: Localization to the nucleus is modulated by the interaction with PIP4K2B (By similarity). Localized to the demarcation membrane system (DMS). May translocate from the cytosol to the cell membrane upon activation of tyrosine phosphorylation. May translocate from the inner to the outer segments of the rod photoreceptor cells in response to light.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 405404Phosphatidylinositol 5-phosphate 4-kinase type-2 alphaPRO_0000185466Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei3 – 31PhosphothreonineBy similarity
Modified residuei14 – 141PhosphoserineBy similarity
Modified residuei89 – 891N6-acetyllysineBy similarity
Modified residuei145 – 1451N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO70172.
PaxDbiO70172.
PeptideAtlasiO70172.
PRIDEiO70172.

PTM databases

iPTMnetiO70172.
PhosphoSiteiO70172.

Expressioni

Tissue specificityi

Detected in rod photoreceptor cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000026737.
ExpressionAtlasiO70172. baseline and differential.
GenevisibleiO70172. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with PIP4K2B. Interaction with PIP4K2B may modulate localization to the nucleus (By similarity).By similarity

Protein-protein interaction databases

IntActiO70172. 3 interactions.
MINTiMINT-4107547.
STRINGi10090.ENSMUSP00000006912.

Structurei

3D structure databases

ProteinModelPortaliO70172.
SMRiO70172. Positions 31-405.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 405373PIPKPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 PIPK domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0229. Eukaryota.
COG5253. LUCA.
HOGENOMiHOG000007832.
HOVERGENiHBG000072.
InParanoidiO70172.
KOiK00920.
OMAiIVECHGV.
OrthoDBiEOG091G0857.
PhylomeDBiO70172.
TreeFamiTF354315.

Family and domain databases

Gene3Di3.30.800.10. 1 hit.
3.30.810.10. 1 hit.
InterProiIPR023610. PInositol-4-P-5-kinase.
IPR027483. PInositol-4-P-5-kinase_C.
IPR002498. PInositol-4-P-5-kinase_core.
IPR027484. PInositol-4-P-5-kinase_N.
[Graphical view]
PANTHERiPTHR23086. PTHR23086. 1 hit.
PfamiPF01504. PIP5K. 1 hit.
[Graphical view]
SMARTiSM00330. PIPKc. 1 hit.
[Graphical view]
PROSITEiPS51455. PIPK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O70172-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATPGNLGSS VLASKTKTKK KHFVAQKVKL FRASDPLLSV LMWGVNHSIN
60 70 80 90 100
ELSHVQIPVM LMPDDFKAYS KIKVDNHLFN KENMPSHFKF KEYCPMVFRN
110 120 130 140 150
LRERFGIDDQ DFQNSLTRSA PLPNDSQARS GARFHTSYDK RYVIKTITSE
160 170 180 190 200
DVAEMHNILK KYHQYIVECH GVTLLPQFLG MYRLNVDGVE IYVIVTRNVF
210 220 230 240 250
SHRLSVYRKY DLKGSTVARE ASDKEKAKEL PTLKDNDFIN EGQKIYIDDN
260 270 280 290 300
NKKIFLEKLK KDVEFLAQLK LMDYSLLVGI HDVERAEQEE VECEENDGEE
310 320 330 340 350
EGESDSTHPI GTPPDSPGNT LNSSPPLAPG EFDPNIDVYA IKCHENAPRK
360 370 380 390 400
EVYFMAIIDI LTHYDAKKKA AHAAKTVKHG AGAEISTVNP EQYSKRFLDF

IGHIL
Length:405
Mass (Da):46,152
Last modified:August 1, 1998 - v1
Checksum:i2D6CC431BDA4D967
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009615 mRNA. Translation: BAA25676.1.
BC011097 mRNA. Translation: AAH11097.1.
CCDSiCCDS15712.1.
RefSeqiNP_032871.3. NM_008845.4.
UniGeneiMm.313977.

Genome annotation databases

EnsembliENSMUST00000006912; ENSMUSP00000006912; ENSMUSG00000026737.
GeneIDi18718.
KEGGimmu:18718.
UCSCiuc008imb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009615 mRNA. Translation: BAA25676.1.
BC011097 mRNA. Translation: AAH11097.1.
CCDSiCCDS15712.1.
RefSeqiNP_032871.3. NM_008845.4.
UniGeneiMm.313977.

3D structure databases

ProteinModelPortaliO70172.
SMRiO70172. Positions 31-405.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO70172. 3 interactions.
MINTiMINT-4107547.
STRINGi10090.ENSMUSP00000006912.

PTM databases

iPTMnetiO70172.
PhosphoSiteiO70172.

Proteomic databases

EPDiO70172.
PaxDbiO70172.
PeptideAtlasiO70172.
PRIDEiO70172.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000006912; ENSMUSP00000006912; ENSMUSG00000026737.
GeneIDi18718.
KEGGimmu:18718.
UCSCiuc008imb.1. mouse.

Organism-specific databases

CTDi5305.
MGIiMGI:1298206. Pip4k2a.

Phylogenomic databases

eggNOGiKOG0229. Eukaryota.
COG5253. LUCA.
HOGENOMiHOG000007832.
HOVERGENiHBG000072.
InParanoidiO70172.
KOiK00920.
OMAiIVECHGV.
OrthoDBiEOG091G0857.
PhylomeDBiO70172.
TreeFamiTF354315.

Enzyme and pathway databases

ReactomeiR-MMU-1660499. Synthesis of PIPs at the plasma membrane.
R-MMU-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.

Miscellaneous databases

PROiO70172.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026737.
ExpressionAtlasiO70172. baseline and differential.
GenevisibleiO70172. MM.

Family and domain databases

Gene3Di3.30.800.10. 1 hit.
3.30.810.10. 1 hit.
InterProiIPR023610. PInositol-4-P-5-kinase.
IPR027483. PInositol-4-P-5-kinase_C.
IPR002498. PInositol-4-P-5-kinase_core.
IPR027484. PInositol-4-P-5-kinase_N.
[Graphical view]
PANTHERiPTHR23086. PTHR23086. 1 hit.
PfamiPF01504. PIP5K. 1 hit.
[Graphical view]
SMARTiSM00330. PIPKc. 1 hit.
[Graphical view]
PROSITEiPS51455. PIPK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPI42A_MOUSE
AccessioniPrimary (citable) accession number: O70172
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.