O70169 (PRS39_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inactive serine protease 39

Alternative name(s):
Inactive testicular serine protease 1

Gene names

Name:	Prss39
Synonyms:	Tesp1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May play an important role in the sperm/egg interaction; released during the acrosome reaction. Ref.1
Subcellular location	Cytoplasmic vesicle › secretory vesicle › acrosome. Secreted Ref.1.
Tissue specificity	Expressed in testis. More specifically, abundantly expressed in the haploid round spermatid. Ref.1
Miscellaneous	There appears to be no human ortholog of this protein.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.
Caution	In contrast to other members of the family, lacks the conserved Asp at position 158, which is replaced by an Asn residue, suggesting it is inactive.

Ontologies

Keywords
Cellular component	Cytoplasmic vesicle Secreted
Domain	Signal
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular_component	acrosomal vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 367	336	Inactive serine protease 39		PRO_0000344981
Regions
	Domain	68 – 312	245	Peptidase S1
Amino acid modifications
	Disulfide bond	93 ↔ 109		By similarity
	Disulfide bond	192 ↔ 269		By similarity
	Disulfide bond	225 ↔ 248		By similarity
	Disulfide bond	259 ↔ 287		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O70169 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 029833E3253CF954	FASTA	367	40,766
	10 20 30 40 50 60 MWGSRAQQSG PDRGGACLLA AFLLCFSLLH AQDYTPSQTP PPTSNTSLKP RGRVQKELCG 70 80 90 100 110 120 KTKFQGKIYG GQIAKAERWP WQASLIFRGR HICGAVLIDK TWLLSAAHCF QRSLTPSDYR 130 140 150 160 170 180 ILLGYNQLSN PSNYSRQMTV NKVILHEDYS KLSRLEKNIV LIQLHHPVIY STHIFPACVP 190 200 210 220 230 240 DGTTKVSPNN LCWISGWGML SADKFLQAPF PLLDAEVSLI DEEECTTFFQ TPEVSITEYD 250 260 270 280 290 300 VIKDDVLCAG DLTNQKSSCR GDSGGPLVCF LNSFWYVVGL ANWNGACLEP IHSPNIFTKV 310 320 330 340 350 360 SYFSDWIKQK KANTPAADVS SAPLEEMASS LRGWGNYSAG ITLKPRISTT LLSSQALLLQ SIWLRIL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two novel testicular serine proteases, TESP1 and TESP2, are present in the mouse sperm acrosome." Kohno N., Yamagata K., Yamada S., Kashiwabara S., Sakai Y., Baba T. Biochem. Biophys. Res. Commun. 245:658-665(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION. Tissue: Testis.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC049616 mRNA. Translation: AAH49616.2. BC115665 mRNA. Translation: AAI15666.1. BC115666 mRNA. Translation: AAI15667.1. AB008910 mRNA. Translation: BAA26132.1.
IPI	IPI00378130.
PIR	JE0104.
RefSeq	NP_033381.1. NM_009355.2.
UniGene	Mm.10725.
3D structure databases
HSSP	HSSP built from PDB template 1CHG based on UniProtKB P00766.
ProteinModelPortal	O70169.
SMR	O70169. Positions 68-312.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000027299.
Protein family/group databases
MEROPS	S01.985.
Proteomic databases
PRIDE	O70169.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000027299; ENSMUSP00000027299; ENSMUSG00000026125.
GeneID	21755.
KEGG	mmu:21755.
UCSC	uc007aov.1. mouse.
Organism-specific databases
CTD	21755.
MGI	MGI:1270856. Prss39.
Phylogenomic databases
eggNOG	COG5640.
GeneTree	ENSGT00700000104082.
HOGENOM	HOG000154548.
HOVERGEN	HBG108561.
InParanoid	O70169.
OMA	SIWLRIL.
OrthoDB	EOG4MCX1B.
Gene expression databases
Bgee	O70169.
Genevestigator	O70169.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	301063.
SOURCE	Search...

Entry information

Entry name

PRS39_MOUSE

Accession

Primary (citable) accession number: O70169
Secondary accession number(s): Q80YD5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	August 1, 1998
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program