DNA topoisomerase 3-alpha - Mus musculus (Mouse)

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O70157 (TOP3A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 109. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
DNA topoisomerase 3-alpha
EC=5.99.1.2

Alternative name(s):
DNA topoisomerase III alpha

Gene names

Name:	Top3a
Synonyms:	Top3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1003 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity By similarity.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Cofactor	Magnesium.
Subunit structure	Directly interacts with BLM and RMI1. Component of the RMI complex, containing at least TOP3A, RMI1 and RMI2. The RMI complex interacts with BLM By similarity.
Tissue specificity	Highly expressed in testis.
Sequence similarities	Belongs to the type IA topoisomerase family. Contains 1 Toprim domain.

Ontologies

Keywords
Domain	Repeat Zinc-finger
Ligand	ATP-binding DNA-binding Magnesium Metal-binding Nucleotide-binding Zinc
Molecular function	Isomerase Topoisomerase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA topological change Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	PML body Inferred from electronic annotation. Source: Compara chromosome Inferred from electronic annotation. Source: InterPro nucleoplasm Traceable author statement. Source: Reactome
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1003

1003

DNA topoisomerase 3-alpha

PRO_0000145191

Regions

Domain

35 – 179

145

Toprim

Repeat

814 – 841

Repeat

898 – 925

Zinc finger

658 – 685

C4-type Potential

Region

814 – 925

112

2 X 27 AA approximate repeats

Sites

Active site

362

O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Amino acid modifications

Modified residue

777

Phosphoserine Ref.2

Modified residue

783

Phosphoserine Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

O70157 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 254C738E746EE495
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FASTA

1,003

112,358

        10         20         30         40         50         60 
MIFPVTLLAF QWHRRPGGRA LSRAAMEVAF RGVRKVLCVA EKNDAAKGIA DLLSNGRMRR 

        70         80         90        100        110        120 
KEGLSKFNKI YEFDYHLYGQ NVTMIMTSVS GHLLAHDFQM QFRKWQSCNP LVLFEAEIEK 

       130        140        150        160        170        180 
YCPENFIDIK KTLERETHHC QALVIWTDCD REGENIGFEI IHVCKAVKPN LRVLRARFSE 

       190        200        210        220        230        240 
ITPHAVRTAC ENLTEPDQRV SDAVDVRQEL DLRIGAAFTR FQTLRLQRIF PEVLAEQLIS 

       250        260        270        280        290        300 
YGSCQFPTLG FVVERFKAIQ AFVPEVFHKI KVTHDHKDGT VEFNWKRYRL FNHTACLVLY 

       310        320        330        340        350        360 
QLCMEDPMAT VVEVRSKPKS KWRPQALDTV ELEKLASRKL RINAKETMRI AEKLYTQGYI 

       370        380        390        400        410        420 
SYPRTETNIF PKDLNLVALV EQQTVDPHWG AFAQTILERG GPTPRNGSKS DQAHPPIHPT 

       430        440        450        460        470        480 
KYTSGLQGDD RRLYEFIVRH FLACCSQDAQ GQETTVEIDI AQERFVAHGL IILARNYLDV 

       490        500        510        520        530        540 
YPYDHWSDKL LPVYEQGSHF QPSTVEMVDG ETSPPQLLTE ADLIALMEKH GIGTDATHAE 

       550        560        570        580        590        600 
HIETIKARMY VGLTSDKRFL PGHLGMGLVE GYDSMGYEMS KPDLRAELEA DLKLICEGKK 

       610        620        630        640        650        660 
DKFQVLRQQV QKYKQVFIEA VAKAKKLDEA LSQYLGERTE MAQQEEIYPA MPEPVRKCPQ 

       670        680        690        700        710        720 
CNKDMVLKTK KSGGFYLSCM GFPECRSAVW FPDSVLEASR DNSVCSVCQP PPVYRLKLKF 

       730        740        750        760        770        780 
KRGSLPPAMP LEFVGCIGGC DETLKEIFGL RFPRALPRAS QPSGHLQASQ ALNRMDSSQH 

       790        800        810        820        830        840 
NLSQPLVNRH TRPSKTVAQA LLPPTTAGES NSVTCNCGRE AVLLTVRKQG PNQGRHFYKC 

       850        860        870        880        890        900 
SNGDCNFFLW ADSSHSTGGG TPTSASGPPG SSVGCPSSVG SHMDGFGSLG SDSDGGTPCL 

       910        920        930        940        950        960 
CGQPAVTRTV QKDGPNKGRQ FHTCAKPREQ QCGFFQWVDE NVAPGSFAAP AWPGGRGKAQ 

       970        980        990       1000 
RPEAASKRPR AGSSDAGSTV KKPRKCSLCH QPGHTRTFCP QNR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of a cDNA encoding mouse DNA topoisomerase III which is highly expressed at the mRNA level in the testis." Seki T., Seki M., Katada T., Enomoto T. Biochim. Biophys. Acta 1396:127-131(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Testis.
[2]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-777 AND SER-783, MASS SPECTROMETRY. Tissue: Embryonic kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB006074 mRNA. Translation: BAA25662.1.
IPI	IPI00115672.
PIR	T13951.
RefSeq	NP_033436.1. NM_009410.2.
UniGene	Mm.477819.
3D structure databases
ProteinModelPortal	O70157.
SMR	O70157. Positions 37-679.
ModBase	Search...
Protein-protein interaction databases
IntAct	O70157. 9 interactions.
PTM databases
PhosphoSite	O70157.
Proteomic databases
PaxDb	O70157.
PRIDE	O70157.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000002891; ENSMUSP00000002891; ENSMUSG00000002814.
GeneID	21975.
KEGG	mmu:21975.
Organism-specific databases
CTD	7156.
MGI	MGI:1197527. Top3a.
Phylogenomic databases
eggNOG	COG0550.
HOGENOM	HOG000184377.
HOVERGEN	HBG056668.
InParanoid	O70157.
KO	K03165.
OMA	KPRKCSL.
Enzyme and pathway databases
Reactome	REACT_120463. Meiosis. REACT_27235. Meiotic Recombination (mouse).
Gene expression databases
ArrayExpress	O70157.
Bgee	O70157.
CleanEx	MM_TOP3A.
Genevestigator	O70157.
GermOnline	ENSMUSG00000002814. Mus musculus.
Family and domain databases
Gene3D	1.10.290.10. 1 hit. 1.10.460.10. 2 hits.
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013826. Topo_IA_cen_sub3. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR006171. Toprim_domain. IPR001878. Znf_CCHC. IPR010666. Znf_GRF. [Graphical view]
PANTHER	PTHR11390. PTHR11390. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 1 hit. PF06839. zf-GRF. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. SM00343. ZnF_C2HC. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. PS50880. TOPRIM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	301678.
SOURCE	Search...

Entry information

Entry name

TOP3A_MOUSE

Accession

Primary (citable) accession number: O70157

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	May 1, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents