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O70157

- TOP3A_MOUSE

UniProt

O70157 - TOP3A_MOUSE

Protein

DNA topoisomerase 3-alpha

Gene

Top3a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity By similarity.By similarity

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

    Cofactori

    Magnesium.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei362 – 3621O-(5'-phospho-DNA)-tyrosine intermediateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri658 – 68528C4-typeSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. DNA topological change Source: UniProtKB

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_198629. Meiotic recombination.
    REACT_27235. Meiotic Recombination.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 3-alpha (EC:5.99.1.2)
    Alternative name(s):
    DNA topoisomerase III alpha
    Gene namesi
    Name:Top3a
    Synonyms:Top3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1197527. Top3a.

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome Source: InterPro
    2. nucleoplasm Source: Reactome
    3. PML body Source: Ensembl

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10031003DNA topoisomerase 3-alphaPRO_0000145191Add
    BLAST

    Proteomic databases

    PaxDbiO70157.
    PRIDEiO70157.

    PTM databases

    PhosphoSiteiO70157.

    Expressioni

    Tissue specificityi

    Highly expressed in testis.

    Gene expression databases

    BgeeiO70157.
    CleanExiMM_TOP3A.
    GenevestigatoriO70157.

    Interactioni

    Subunit structurei

    Interacts (via N-terminal region) with BLM; the interaction is direct. Directly interacts with RMI1. Component of the RMI complex, containing at least TOP3A, RMI1 and RMI2. The RMI complex interacts with BLM By similarity.By similarity

    Protein-protein interaction databases

    BioGridi204278. 8 interactions.
    IntActiO70157. 9 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliO70157.
    SMRiO70157. Positions 21-679.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini35 – 179145ToprimPROSITE-ProRule annotationAdd
    BLAST
    Repeati814 – 841281Add
    BLAST
    Repeati898 – 925282Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni814 – 9251122 X 27 AA approximate repeatsAdd
    BLAST

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.Curated
    Contains 1 Toprim domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri658 – 68528C4-typeSequence AnalysisAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0550.
    HOGENOMiHOG000184377.
    HOVERGENiHBG056668.
    InParanoidiO70157.
    KOiK03165.
    OMAiLYQICME.
    OrthoDBiEOG74R1Q0.
    PhylomeDBiO70157.
    TreeFamiTF105287.

    Family and domain databases

    Gene3Di1.10.290.10. 1 hit.
    1.10.460.10. 2 hits.
    3.40.50.140. 1 hit.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013826. Topo_IA_cen_sub3.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR006171. Toprim_domain.
    IPR001878. Znf_CCHC.
    IPR010666. Znf_GRF.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 1 hit.
    PF06839. zf-GRF. 2 hits.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    SM00343. ZnF_C2HC. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O70157-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIFPVTLLAF QWHRRPGGRA LSRAAMEVAF RGVRKVLCVA EKNDAAKGIA     50
    DLLSNGRMRR KEGLSKFNKI YEFDYHLYGQ NVTMIMTSVS GHLLAHDFQM 100
    QFRKWQSCNP LVLFEAEIEK YCPENFIDIK KTLERETHHC QALVIWTDCD 150
    REGENIGFEI IHVCKAVKPN LRVLRARFSE ITPHAVRTAC ENLTEPDQRV 200
    SDAVDVRQEL DLRIGAAFTR FQTLRLQRIF PEVLAEQLIS YGSCQFPTLG 250
    FVVERFKAIQ AFVPEVFHKI KVTHDHKDGT VEFNWKRYRL FNHTACLVLY 300
    QLCMEDPMAT VVEVRSKPKS KWRPQALDTV ELEKLASRKL RINAKETMRI 350
    AEKLYTQGYI SYPRTETNIF PKDLNLVALV EQQTVDPHWG AFAQTILERG 400
    GPTPRNGSKS DQAHPPIHPT KYTSGLQGDD RRLYEFIVRH FLACCSQDAQ 450
    GQETTVEIDI AQERFVAHGL IILARNYLDV YPYDHWSDKL LPVYEQGSHF 500
    QPSTVEMVDG ETSPPQLLTE ADLIALMEKH GIGTDATHAE HIETIKARMY 550
    VGLTSDKRFL PGHLGMGLVE GYDSMGYEMS KPDLRAELEA DLKLICEGKK 600
    DKFQVLRQQV QKYKQVFIEA VAKAKKLDEA LSQYLGERTE MAQQEEIYPA 650
    MPEPVRKCPQ CNKDMVLKTK KSGGFYLSCM GFPECRSAVW FPDSVLEASR 700
    DNSVCSVCQP PPVYRLKLKF KRGSLPPAMP LEFVGCIGGC DETLKEIFGL 750
    RFPRALPRAS QPSGHLQASQ ALNRMDSSQH NLSQPLVNRH TRPSKTVAQA 800
    LLPPTTAGES NSVTCNCGRE AVLLTVRKQG PNQGRHFYKC SNGDCNFFLW 850
    ADSSHSTGGG TPTSASGPPG SSVGCPSSVG SHMDGFGSLG SDSDGGTPCL 900
    CGQPAVTRTV QKDGPNKGRQ FHTCAKPREQ QCGFFQWVDE NVAPGSFAAP 950
    AWPGGRGKAQ RPEAASKRPR AGSSDAGSTV KKPRKCSLCH QPGHTRTFCP 1000
    QNR 1003
    Length:1,003
    Mass (Da):112,358
    Last modified:August 1, 1998 - v1
    Checksum:i254C738E746EE495
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB006074 mRNA. Translation: BAA25662.1.
    CCDSiCCDS24797.1.
    PIRiT13951.
    RefSeqiNP_033436.1. NM_009410.2.
    UniGeneiMm.477819.

    Genome annotation databases

    EnsembliENSMUST00000002891; ENSMUSP00000002891; ENSMUSG00000002814.
    GeneIDi21975.
    KEGGimmu:21975.
    UCSCiuc007jgl.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB006074 mRNA. Translation: BAA25662.1 .
    CCDSi CCDS24797.1.
    PIRi T13951.
    RefSeqi NP_033436.1. NM_009410.2.
    UniGenei Mm.477819.

    3D structure databases

    ProteinModelPortali O70157.
    SMRi O70157. Positions 21-679.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204278. 8 interactions.
    IntActi O70157. 9 interactions.

    PTM databases

    PhosphoSitei O70157.

    Proteomic databases

    PaxDbi O70157.
    PRIDEi O70157.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000002891 ; ENSMUSP00000002891 ; ENSMUSG00000002814 .
    GeneIDi 21975.
    KEGGi mmu:21975.
    UCSCi uc007jgl.1. mouse.

    Organism-specific databases

    CTDi 7156.
    MGIi MGI:1197527. Top3a.

    Phylogenomic databases

    eggNOGi COG0550.
    HOGENOMi HOG000184377.
    HOVERGENi HBG056668.
    InParanoidi O70157.
    KOi K03165.
    OMAi LYQICME.
    OrthoDBi EOG74R1Q0.
    PhylomeDBi O70157.
    TreeFami TF105287.

    Enzyme and pathway databases

    Reactomei REACT_198629. Meiotic recombination.
    REACT_27235. Meiotic Recombination.

    Miscellaneous databases

    NextBioi 301678.
    PROi O70157.
    SOURCEi Search...

    Gene expression databases

    Bgeei O70157.
    CleanExi MM_TOP3A.
    Genevestigatori O70157.

    Family and domain databases

    Gene3Di 1.10.290.10. 1 hit.
    1.10.460.10. 2 hits.
    3.40.50.140. 1 hit.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013826. Topo_IA_cen_sub3.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR006171. Toprim_domain.
    IPR001878. Znf_CCHC.
    IPR010666. Znf_GRF.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 1 hit.
    PF06839. zf-GRF. 2 hits.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    SM00343. ZnF_C2HC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of a cDNA encoding mouse DNA topoisomerase III which is highly expressed at the mRNA level in the testis."
      Seki T., Seki M., Katada T., Enomoto T.
      Biochim. Biophys. Acta 1396:127-131(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Testis.

    Entry informationi

    Entry nameiTOP3A_MOUSE
    AccessioniPrimary (citable) accession number: O70157
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3