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O70157

- TOP3A_MOUSE

UniProt

O70157 - TOP3A_MOUSE

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Protein

DNA topoisomerase 3-alpha

Gene
Top3a, Top3
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity By similarity.

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Cofactori

Magnesium.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei362 – 3621O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri658 – 68528C4-type Reviewed predictionAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: UniProtKB
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. DNA topological change Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_198629. Meiotic recombination.
REACT_27235. Meiotic Recombination.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3-alpha (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase III alpha
Gene namesi
Name:Top3a
Synonyms:Top3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:1197527. Top3a.

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
  2. nucleoplasm Source: Reactome
  3. PML body Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10031003DNA topoisomerase 3-alphaPRO_0000145191Add
BLAST

Proteomic databases

PaxDbiO70157.
PRIDEiO70157.

PTM databases

PhosphoSiteiO70157.

Expressioni

Tissue specificityi

Highly expressed in testis.

Gene expression databases

BgeeiO70157.
CleanExiMM_TOP3A.
GenevestigatoriO70157.

Interactioni

Subunit structurei

Interacts (via N-terminal region) with BLM; the interaction is direct. Directly interacts with RMI1. Component of the RMI complex, containing at least TOP3A, RMI1 and RMI2. The RMI complex interacts with BLM By similarity.

Protein-protein interaction databases

BioGridi204278. 8 interactions.
IntActiO70157. 9 interactions.

Structurei

3D structure databases

ProteinModelPortaliO70157.
SMRiO70157. Positions 21-679.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 179145ToprimAdd
BLAST
Repeati814 – 841281Add
BLAST
Repeati898 – 925282Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni814 – 9251122 X 27 AA approximate repeatsAdd
BLAST

Sequence similaritiesi

Contains 1 Toprim domain.

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri658 – 68528C4-type Reviewed predictionAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0550.
HOGENOMiHOG000184377.
HOVERGENiHBG056668.
InParanoidiO70157.
KOiK03165.
OMAiLYQICME.
OrthoDBiEOG74R1Q0.
PhylomeDBiO70157.
TreeFamiTF105287.

Family and domain databases

Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR006171. Toprim_domain.
IPR001878. Znf_CCHC.
IPR010666. Znf_GRF.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 1 hit.
PF06839. zf-GRF. 2 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
SM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O70157-1 [UniParc]FASTAAdd to Basket

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MIFPVTLLAF QWHRRPGGRA LSRAAMEVAF RGVRKVLCVA EKNDAAKGIA     50
DLLSNGRMRR KEGLSKFNKI YEFDYHLYGQ NVTMIMTSVS GHLLAHDFQM 100
QFRKWQSCNP LVLFEAEIEK YCPENFIDIK KTLERETHHC QALVIWTDCD 150
REGENIGFEI IHVCKAVKPN LRVLRARFSE ITPHAVRTAC ENLTEPDQRV 200
SDAVDVRQEL DLRIGAAFTR FQTLRLQRIF PEVLAEQLIS YGSCQFPTLG 250
FVVERFKAIQ AFVPEVFHKI KVTHDHKDGT VEFNWKRYRL FNHTACLVLY 300
QLCMEDPMAT VVEVRSKPKS KWRPQALDTV ELEKLASRKL RINAKETMRI 350
AEKLYTQGYI SYPRTETNIF PKDLNLVALV EQQTVDPHWG AFAQTILERG 400
GPTPRNGSKS DQAHPPIHPT KYTSGLQGDD RRLYEFIVRH FLACCSQDAQ 450
GQETTVEIDI AQERFVAHGL IILARNYLDV YPYDHWSDKL LPVYEQGSHF 500
QPSTVEMVDG ETSPPQLLTE ADLIALMEKH GIGTDATHAE HIETIKARMY 550
VGLTSDKRFL PGHLGMGLVE GYDSMGYEMS KPDLRAELEA DLKLICEGKK 600
DKFQVLRQQV QKYKQVFIEA VAKAKKLDEA LSQYLGERTE MAQQEEIYPA 650
MPEPVRKCPQ CNKDMVLKTK KSGGFYLSCM GFPECRSAVW FPDSVLEASR 700
DNSVCSVCQP PPVYRLKLKF KRGSLPPAMP LEFVGCIGGC DETLKEIFGL 750
RFPRALPRAS QPSGHLQASQ ALNRMDSSQH NLSQPLVNRH TRPSKTVAQA 800
LLPPTTAGES NSVTCNCGRE AVLLTVRKQG PNQGRHFYKC SNGDCNFFLW 850
ADSSHSTGGG TPTSASGPPG SSVGCPSSVG SHMDGFGSLG SDSDGGTPCL 900
CGQPAVTRTV QKDGPNKGRQ FHTCAKPREQ QCGFFQWVDE NVAPGSFAAP 950
AWPGGRGKAQ RPEAASKRPR AGSSDAGSTV KKPRKCSLCH QPGHTRTFCP 1000
QNR 1003
Length:1,003
Mass (Da):112,358
Last modified:August 1, 1998 - v1
Checksum:i254C738E746EE495
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB006074 mRNA. Translation: BAA25662.1.
CCDSiCCDS24797.1.
PIRiT13951.
RefSeqiNP_033436.1. NM_009410.2.
UniGeneiMm.477819.

Genome annotation databases

EnsembliENSMUST00000002891; ENSMUSP00000002891; ENSMUSG00000002814.
GeneIDi21975.
KEGGimmu:21975.
UCSCiuc007jgl.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB006074 mRNA. Translation: BAA25662.1 .
CCDSi CCDS24797.1.
PIRi T13951.
RefSeqi NP_033436.1. NM_009410.2.
UniGenei Mm.477819.

3D structure databases

ProteinModelPortali O70157.
SMRi O70157. Positions 21-679.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204278. 8 interactions.
IntActi O70157. 9 interactions.

PTM databases

PhosphoSitei O70157.

Proteomic databases

PaxDbi O70157.
PRIDEi O70157.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000002891 ; ENSMUSP00000002891 ; ENSMUSG00000002814 .
GeneIDi 21975.
KEGGi mmu:21975.
UCSCi uc007jgl.1. mouse.

Organism-specific databases

CTDi 7156.
MGIi MGI:1197527. Top3a.

Phylogenomic databases

eggNOGi COG0550.
HOGENOMi HOG000184377.
HOVERGENi HBG056668.
InParanoidi O70157.
KOi K03165.
OMAi LYQICME.
OrthoDBi EOG74R1Q0.
PhylomeDBi O70157.
TreeFami TF105287.

Enzyme and pathway databases

Reactomei REACT_198629. Meiotic recombination.
REACT_27235. Meiotic Recombination.

Miscellaneous databases

NextBioi 301678.
PROi O70157.
SOURCEi Search...

Gene expression databases

Bgeei O70157.
CleanExi MM_TOP3A.
Genevestigatori O70157.

Family and domain databases

Gene3Di 1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR006171. Toprim_domain.
IPR001878. Znf_CCHC.
IPR010666. Znf_GRF.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 1 hit.
PF06839. zf-GRF. 2 hits.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
SM00343. ZnF_C2HC. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isolation of a cDNA encoding mouse DNA topoisomerase III which is highly expressed at the mRNA level in the testis."
    Seki T., Seki M., Katada T., Enomoto T.
    Biochim. Biophys. Acta 1396:127-131(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Testis.

Entry informationi

Entry nameiTOP3A_MOUSE
AccessioniPrimary (citable) accession number: O70157
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: September 3, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi