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O70156 (OLR1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Oxidized low-density lipoprotein receptor 1

Short name=Ox-LDL receptor 1
Alternative name(s):
Lectin-like oxidized LDL receptor 1
Short name=LOX-1
Short name=Lectin-like oxLDL receptor 1
Lectin-type oxidized LDL receptor 1
Gene names
Name:Olr1
Synonyms:Lox1, Oldlr1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length364 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria. Ref.2 Ref.4

Subunit structure

Homodimer; disulfide-linked. May form a hexamer composed of 3 homodimers. Interacts with HSP70 By similarity.

Subcellular location

Cell membrane; Lipid-anchor By similarity. Cell membrane; Single-pass type II membrane protein By similarity. Membrane raft By similarity. Secreted By similarity. Note: A secreted form also exists. Localization to membrane rafts requires palmitoylation By similarity.

Tissue specificity

Predominantly expressed in lung and at lower level in kidney. Expressed in macrophages but not in vascular smooth muscle cells. Ref.1

Induction

By hypertension. Up-regulated by shear stress, lipopolysaccharide and TNF-alpha in cultured vascular endothelial cells. Ref.1 Ref.2

Domain

The cytoplasmic region is required for subcellular sorting on the cell surface By similarity.

The C-type lectin domain mediates the recognition and binding of oxLDL By similarity.

The Neck region contains 3 internal repeats that are only found in rodents.

Post-translational modification

N-glycosylated By similarity.

Sequence similarities

Contains 1 C-type lectin domain.

Ontologies

Keywords
   Biological processCell adhesion
Immunity
Inflammatory response
   Cellular componentCell membrane
Membrane
Secreted
   DomainCoiled coil
Repeat
Signal-anchor
Transmembrane
Transmembrane helix
   LigandLectin
   Molecular functionReceptor
   PTMDisulfide bond
Glycoprotein
Lipoprotein
Palmitate
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell death

Inferred from mutant phenotype PubMed 12435393. Source: RGD

immune system process

Inferred from electronic annotation. Source: UniProtKB-KW

inflammatory response

Inferred from mutant phenotype Ref.4. Source: RGD

leukocyte cell-cell adhesion

Inferred from mutant phenotype Ref.4. Source: RGD

lipoprotein metabolic process

Inferred from mutant phenotype PubMed 12435393. Source: RGD

receptor-mediated endocytosis

Traceable author statement PubMed 12384456. Source: GOC

response to hydrogen peroxide

Inferred from expression pattern PubMed 16055083. Source: RGD

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

membrane raft

Inferred from electronic annotation. Source: UniProtKB-SubCell

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncarbohydrate binding

Inferred from electronic annotation. Source: InterPro

low-density lipoprotein receptor activity

Traceable author statement PubMed 12384456. Source: RGD

receptor activity

Traceable author statement Ref.1. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 364364Oxidized low-density lipoprotein receptor 1
PRO_0000017451
Chain? – 364Oxidized low-density lipoprotein receptor 1, soluble formPRO_0000017452

Regions

Topological domain1 – 3131Cytoplasmic Potential
Transmembrane32 – 5423Helical; Signal-anchor for type II membrane protein; Potential
Topological domain55 – 364310Extracellular Potential
Repeat96 – 141461
Repeat142 – 187462
Repeat188 – 233463
Domain242 – 355114C-type lectin
Region55 – 242188Neck
Coiled coil83 – 233151 Potential

Amino acid modifications

Lipidation351S-palmitoyl cysteine By similarity
Lipidation451S-palmitoyl cysteine By similarity
Glycosylation721N-linked (GlcNAc...) Potential
Glycosylation921N-linked (GlcNAc...) Potential
Glycosylation1381N-linked (GlcNAc...) Potential
Disulfide bond235 ↔ 246 By similarity
Disulfide bond262 ↔ 354 By similarity
Disulfide bond333 ↔ 346 By similarity

Sequences

Sequence LengthMass (Da)Tools
O70156 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 0AD2839C07206E09

FASTA36441,890
        10         20         30         40         50         60 
MAFDDKMKPV NGQPDQKSCG KKPKGLHLLS STWWCPAAVT LAILCLVLSV TLIVQQTQLL 

        70         80         90        100        110        120 
QVSDLLKQYQ ANLTQQDHIL EGQMSAQKKA ENASQESKRE LKEQIDTLTW KLNEKSKEQE 

       130        140        150        160        170        180 
KLLQQNQNLQ EALQRAVNAS EESKWELKEQ IDILNWKLNG ISKEQKELLQ QNQNLQEALQ 

       190        200        210        220        230        240 
KAEKYSEESQ RELKEQIDTL SWKLNEKSKE QEELLQQNQN LQEALQRAAN SSGPCPQDWI 

       250        260        270        280        290        300 
WHKENCYLFH GPFNWEKSRE NCLSLDAQLL QISTTDDLNF VLQATSHSTS PFWMGLHRKN 

       310        320        330        340        350        360 
PNHPWLWENG SPLSFQFFRT RGVSLQMYSS GTCAYIQGGV VFAENCILTA FSICQKKANL 


LLTQ 

« Hide

References

« Hide 'large scale' references
[1]"Unique repetitive sequence and unexpected regulation of expression of rat endothelial receptor for oxidized low-density lipoprotein (LOX-1)."
Nagase M., Hirose S., Fujita T.
Biochem. J. 330:1417-1422(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
Strain: SHR.
Tissue: Kidney.
[2]"Genomic organization and regulation of expression of the lectin-like oxidized low-density lipoprotein receptor (LOX-1) gene."
Nagase M., Abe J., Takahashi K., Ando J., Hirose S., Fujita T.
J. Biol. Chem. 273:33702-33707(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION.
Strain: Sprague-Dawley.
Tissue: Liver.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Placenta.
[4]"Lectin-like oxidized LDL receptor-1 is a cell-adhesion molecule involved in endotoxin-induced inflammation."
Honjo M., Nakamura K., Yamashiro K., Kiryu J., Tanihara H., McEvoy L.M., Honda Y., Butcher E.C., Masaki T., Sawamura T.
Proc. Natl. Acad. Sci. U.S.A. 100:1274-1279(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB005900 mRNA. Translation: BAA25785.1.
AB018104 Genomic DNA. Translation: BAA35123.1.
BC097290 mRNA. Translation: AAH97290.1.
RefSeqNP_579840.2. NM_133306.2.
UniGeneRn.87449.

3D structure databases

ProteinModelPortalO70156.
SMRO70156. Positions 227-360.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000011196.

Proteomic databases

PRIDEO70156.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000011196; ENSRNOP00000011196; ENSRNOG00000008375.
GeneID140914.
KEGGrno:140914.
UCSCRGD:620515. rat.

Organism-specific databases

CTD4973.
RGD620515. Olr1.

Phylogenomic databases

eggNOGNOG242244.
GeneTreeENSGT00700000104266.
HOGENOMHOG000220927.
HOVERGENHBG056863.
InParanoidO70156.
KOK08763.
OMASICQKKA.
OrthoDBEOG747PJT.
PhylomeDBO70156.
TreeFamTF336674.

Gene expression databases

GenevestigatorO70156.

Family and domain databases

Gene3D3.10.100.10. 1 hit.
InterProIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR016187. C-type_lectin_fold.
[Graphical view]
PfamPF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMSSF56436. SSF56436. 1 hit.
PROSITEPS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio620753.
PROO70156.

Entry information

Entry nameOLR1_RAT
AccessionPrimary (citable) accession number: O70156
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: August 1, 1998
Last modified: April 16, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families