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Protein

Calcium/calmodulin-dependent protein kinase type 1B

Gene

Pnck

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro, isoform 1 and isoform 2 phosphorylate CREB1, SYN1/synapsin I. Phosphorylates and activates CAMK1.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by Ca2+/calmodulin. Must be phosphorylated to be maximally active. Activated by CAMKK1.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei44ATPPROSITE-ProRule annotation1
Active sitei136Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi21 – 29ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calmodulin-dependent protein kinase activity Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Calmodulin-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium/calmodulin-dependent protein kinase type 1B (EC:2.7.11.17)
Alternative name(s):
CaM kinase I beta
Short name:
CaM kinase IB
Short name:
CaM-KI beta
Short name:
CaMKI-beta
Pregnancy up-regulated non-ubiquitously-expressed CaM kinase homolog
Gene namesi
Name:Pnck
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi69249. Pnck.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000860811 – 343Calcium/calmodulin-dependent protein kinase type 1BAdd BLAST343

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei338PhosphoserineBy similarity1

Post-translational modificationi

Isoform 1 and isoform 2 are phosphorylated by CAMKK1.Curated

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO70150.

PTM databases

iPTMnetiO70150.
PhosphoSitePlusiO70150.

Expressioni

Tissue specificityi

Isoform 1 is expressed in liver, heart, lung, kidney, spleen and testis. Isoform 2 is predominantly expressed in cerebrum and cerebellum.1 Publication

Gene expression databases

BgeeiENSRNOG00000017923.
GenevisibleiO70150. RN.

Interactioni

Protein-protein interaction databases

BioGridi248282. 1 interactor.
STRINGi10116.ENSRNOP00000024227.

Structurei

3D structure databases

ProteinModelPortaliO70150.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 270Protein kinasePROSITE-ProRule annotationAdd BLAST256

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni290 – 311Calmodulin-bindingBy similarityAdd BLAST22

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
GeneTreeiENSGT00760000118944.
HOGENOMiHOG000233016.
HOVERGENiHBG108055.
InParanoidiO70150.
KOiK08795.
OMAiKNFARMH.
OrthoDBiEOG091G0HK9.
PhylomeDBiO70150.
TreeFamiTF314166.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O70150-1) [UniParc]FASTAAdd to basket
Also known as: CamKIbeta2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLLKKQTED ISSVYEIREK LGSGAFSEVM LAQERGSAHL VALKCIPKKA
60 70 80 90 100
LRGKEALVEN EIAVLRRISH PNIVALEDVH ESPSHLYLAM ELVTGGELFD
110 120 130 140 150
RIMERGSYTE KDASHLVGQV LGAVSYLHSL GIVHRDLKPE NLLYATPFED
160 170 180 190 200
SKIMVSDFGL SKIQAGNMLG TACGTPGYVA PELLEQKPYG KAVDVWALGV
210 220 230 240 250
ISYILLCGYP PFYDESDPEL FSQILRASYE FDSPFWDDIS ESAKDFIRHL
260 270 280 290 300
LERDPQKRFT CQQALQHLWI SGDAALDRDI LGSVSEQIQK NFARTHWKRA
310 320 330 340
FNATSFLRHI RKLGQSPEGE EASRQGMTRH SHPGLGTSQS PKW
Length:343
Mass (Da):38,439
Last modified:August 1, 1998 - v1
Checksum:i994D451D809A9D80
GO
Isoform 2 (identifier: O70150-2) [UniParc]FASTAAdd to basket
Also known as: CamKIbeta1

The sequence of this isoform differs from the canonical sequence as follows:
     319-343: GEEASRQGMTRHSHPGLGTSQSPKW → ALLISFPDPCPPDWPLLESPRPWV

Show »
Length:342
Mass (Da):38,463
Checksum:i546C464913E784A2
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012636319 – 343GEEAS…QSPKW → ALLISFPDPCPPDWPLLESP RPWV in isoform 2. 1 PublicationAdd BLAST25

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86556 mRNA. Translation: BAA19879.1.
AB004267 mRNA. Translation: BAA28263.1.
RefSeqiNP_058971.1. NM_017275.1. [O70150-1]
XP_006229635.1. XM_006229573.3. [O70150-1]
XP_006229636.1. XM_006229574.3. [O70150-1]
XP_006229637.1. XM_006229575.3. [O70150-1]
XP_008771850.1. XM_008773628.2. [O70150-1]
XP_017457441.1. XM_017601952.1. [O70150-2]
UniGeneiRn.11178.

Genome annotation databases

EnsembliENSRNOT00000090795; ENSRNOP00000074179; ENSRNOG00000058317. [O70150-1]
GeneIDi29660.
KEGGirno:29660.
UCSCiRGD:69249. rat. [O70150-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86556 mRNA. Translation: BAA19879.1.
AB004267 mRNA. Translation: BAA28263.1.
RefSeqiNP_058971.1. NM_017275.1. [O70150-1]
XP_006229635.1. XM_006229573.3. [O70150-1]
XP_006229636.1. XM_006229574.3. [O70150-1]
XP_006229637.1. XM_006229575.3. [O70150-1]
XP_008771850.1. XM_008773628.2. [O70150-1]
XP_017457441.1. XM_017601952.1. [O70150-2]
UniGeneiRn.11178.

3D structure databases

ProteinModelPortaliO70150.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248282. 1 interactor.
STRINGi10116.ENSRNOP00000024227.

PTM databases

iPTMnetiO70150.
PhosphoSitePlusiO70150.

Proteomic databases

PaxDbiO70150.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000090795; ENSRNOP00000074179; ENSRNOG00000058317. [O70150-1]
GeneIDi29660.
KEGGirno:29660.
UCSCiRGD:69249. rat. [O70150-1]

Organism-specific databases

CTDi139728.
RGDi69249. Pnck.

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
GeneTreeiENSGT00760000118944.
HOGENOMiHOG000233016.
HOVERGENiHBG108055.
InParanoidiO70150.
KOiK08795.
OMAiKNFARMH.
OrthoDBiEOG091G0HK9.
PhylomeDBiO70150.
TreeFamiTF314166.

Miscellaneous databases

PROiO70150.

Gene expression databases

BgeeiENSRNOG00000017923.
GenevisibleiO70150. RN.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKCC1B_RAT
AccessioniPrimary (citable) accession number: O70150
Secondary accession number(s): O08767
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: August 1, 1998
Last modified: November 30, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.