O70150 (KCC1B_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calcium/calmodulin-dependent protein kinase type 1B
EC=2.7.11.17

Alternative name(s):
CaM kinase I beta
Short name=CaM kinase IB
Short name=CaM-KI beta
Short name=CaMKI-beta
Pregnancy up-regulated non-ubiquitously-expressed CaM kinase homolog

Gene names

Name:

Pnck

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro, isoform 1 and isoform 2 phosphorylate CREB1, SYN1/synapsin I. Phosphorylates and activates CAMK1. Ref.2
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Activated by Ca2+/calmodulin. Must be phosphorylated to be maximally active. Activated by CAMKK1. Ref.2
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Tissue specificity	Isoform 1 is expressed in liver, heart, lung, kidney, spleen and testis. Isoform 2 is predominantly expressed in cerebrum and cerebellum. Ref.2
Post-translational modification	Isoform 1 and isoform 2 are phosphorylated by CAMKK1 Probable.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Calcium Calmodulin-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	calcium- and calmodulin-dependent protein kinase complex Traceable author statement. Source: UniProtKB cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin-dependent protein kinase activity Inferred from direct assay Ref.2. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O70150-1)

Also known as: CamKIbeta2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O70150-2)

Also known as: CamKIbeta1;

The sequence of this isoform differs from the canonical sequence as follows:
     319-343: GEEASRQGMTRHSHPGLGTSQSPKW → ALLISFPDPCPPDWPLLESPRPWV

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Calcium/calmodulin-dependent protein kinase type 1B		PRO_0000086081
Regions
	Domain	15 – 270	256	Protein kinase
	Nucleotide binding	21 – 29	9	ATP By similarity
	Region	290 – 311	22	Calmodulin-binding By similarity
Sites
	Active site	136	1	Proton acceptor By similarity
	Binding site	44	1	ATP By similarity
Natural variations
	Alternative sequence	319 – 343	25	GEEAS…QSPKW → ALLISFPDPCPPDWPLLESP RPWV in isoform 2.		VSP_012636

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (CamKIbeta2) [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 994D451D809A9D80	FASTA	343	38,439
	10 20 30 40 50 60 MLLLKKQTED ISSVYEIREK LGSGAFSEVM LAQERGSAHL VALKCIPKKA LRGKEALVEN 70 80 90 100 110 120 EIAVLRRISH PNIVALEDVH ESPSHLYLAM ELVTGGELFD RIMERGSYTE KDASHLVGQV 130 140 150 160 170 180 LGAVSYLHSL GIVHRDLKPE NLLYATPFED SKIMVSDFGL SKIQAGNMLG TACGTPGYVA 190 200 210 220 230 240 PELLEQKPYG KAVDVWALGV ISYILLCGYP PFYDESDPEL FSQILRASYE FDSPFWDDIS 250 260 270 280 290 300 ESAKDFIRHL LERDPQKRFT CQQALQHLWI SGDAALDRDI LGSVSEQIQK NFARTHWKRA 310 320 330 340 FNATSFLRHI RKLGQSPEGE EASRQGMTRH SHPGLGTSQS PKW
	Isoform 2 (CamKIbeta1) [UniParc]. Checksum: 546C464913E784A2	FASTA	342	38,463
	10 20 30 40 50 60 MLLLKKQTED ISSVYEIREK LGSGAFSEVM LAQERGSAHL VALKCIPKKA LRGKEALVEN 70 80 90 100 110 120 EIAVLRRISH PNIVALEDVH ESPSHLYLAM ELVTGGELFD RIMERGSYTE KDASHLVGQV 130 140 150 160 170 180 LGAVSYLHSL GIVHRDLKPE NLLYATPFED SKIMVSDFGL SKIQAGNMLG TACGTPGYVA 190 200 210 220 230 240 PELLEQKPYG KAVDVWALGV ISYILLCGYP PFYDESDPEL FSQILRASYE FDSPFWDDIS 250 260 270 280 290 300 ESAKDFIRHL LERDPQKRFT CQQALQHLWI SGDAALDRDI LGSVSEQIQK NFARTHWKRA 310 320 330 340 FNATSFLRHI RKLGQSPEAL LISFPDPCPP DWPLLESPRP WV

References

[1]	"Isolation and comparison of rat cDNAs encoding Ca2+/calmodulin-dependent protein kinase I isoforms." Yokokura H., Terada O., Naito Y., Hidaka H. Biochim. Biophys. Acta 1338:8-12(1997) [PubMed: 9074610] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Brain.
[2]	"Isoform-specific activation and structure diversity of calmodulin kinase I." Naito Y., Watanabe Y., Yokokura H., Sugita R., Nishio M., Hidaka H. J. Biol. Chem. 272:32704-32708(1997) [PubMed: 9405489] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D86556 mRNA. Translation: BAA19879.1. AB004267 mRNA. Translation: BAA28263.1.
IPI	IPI00195651. IPI00195716.
RefSeq	NP_058971.1. NM_017275.1. XP_002730347.1. XM_002730301.1.
UniGene	Rn.11178.
3D structure databases
ProteinModelPortal	O70150.
SMR	O70150. Positions 6-310.
ModBase	Search...
Protein-protein interaction databases
STRING	O70150.
PTM databases
PhosphoSite	O70150.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000024227; ENSRNOP00000024227; ENSRNOG00000017923.
GeneID	100360379. 29660.
KEGG	rno:100360379. rno:29660.
UCSC	D86556. rat. NM_017275. rat.
Organism-specific databases
CTD	139728.
RGD	69249. Pnck.
Phylogenomic databases
eggNOG	roNOG12303.
GeneTree	ENSGT00600000084207.
HOVERGEN	HBG108055.
InParanoid	O70150.
OMA	IYESPTH.
OrthoDB	EOG4T4CVW.
PhylomeDB	O70150.
Gene expression databases
ArrayExpress	O70150.
Genevestigator	O70150.
GermOnline	ENSRNOG00000017923. Rattus norvegicus.
Family and domain databases
InterPro	IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
KO	K08795.
PANTHER	PTHR24347. PTHR24347. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	609959.

Entry information

Entry name

KCC1B_RAT

Accession

Primary (citable) accession number: O70150
Secondary accession number(s): O08767

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	August 1, 1998
Last modified:	November 16, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program