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O70150

- KCC1B_RAT

UniProt

O70150 - KCC1B_RAT

Protein

Calcium/calmodulin-dependent protein kinase type 1B

Gene

Pnck

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro, isoform 1 and isoform 2 phosphorylate CREB1, SYN1/synapsin I. Phosphorylates and activates CAMK1.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Activated by Ca2+/calmodulin. Must be phosphorylated to be maximally active. Activated by CAMKK1.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei44 – 441ATPPROSITE-ProRule annotation
    Active sitei136 – 1361Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi21 – 299ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calmodulin-dependent protein kinase activity Source: RGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Calcium, Calmodulin-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calcium/calmodulin-dependent protein kinase type 1B (EC:2.7.11.17)
    Alternative name(s):
    CaM kinase I beta
    Short name:
    CaM kinase IB
    Short name:
    CaM-KI beta
    Short name:
    CaMKI-beta
    Pregnancy up-regulated non-ubiquitously-expressed CaM kinase homolog
    Gene namesi
    Name:Pnck
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi69249. Pnck.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. calcium- and calmodulin-dependent protein kinase complex Source: UniProtKB
    2. cytoplasm Source: UniProtKB-SubCell
    3. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 343343Calcium/calmodulin-dependent protein kinase type 1BPRO_0000086081Add
    BLAST

    Post-translational modificationi

    Isoform 1 and isoform 2 are phosphorylated by CAMKK1.Curated

    PTM databases

    PhosphoSiteiO70150.

    Expressioni

    Tissue specificityi

    Isoform 1 is expressed in liver, heart, lung, kidney, spleen and testis. Isoform 2 is predominantly expressed in cerebrum and cerebellum.1 Publication

    Gene expression databases

    GenevestigatoriO70150.

    Interactioni

    Protein-protein interaction databases

    BioGridi248282. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliO70150.
    SMRiO70150. Positions 6-310.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini15 – 270256Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni290 – 31122Calmodulin-bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00740000115341.
    HOGENOMiHOG000233016.
    HOVERGENiHBG108055.
    InParanoidiO70150.
    KOiK08795.
    OMAiPQKRFTC.
    OrthoDBiEOG7WHH9K.
    PhylomeDBiO70150.
    TreeFamiTF314166.

    Family and domain databases

    InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PANTHERiPTHR24347. PTHR24347. 1 hit.
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O70150-1) [UniParc]FASTAAdd to Basket

    Also known as: CamKIbeta2

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLLLKKQTED ISSVYEIREK LGSGAFSEVM LAQERGSAHL VALKCIPKKA    50
    LRGKEALVEN EIAVLRRISH PNIVALEDVH ESPSHLYLAM ELVTGGELFD 100
    RIMERGSYTE KDASHLVGQV LGAVSYLHSL GIVHRDLKPE NLLYATPFED 150
    SKIMVSDFGL SKIQAGNMLG TACGTPGYVA PELLEQKPYG KAVDVWALGV 200
    ISYILLCGYP PFYDESDPEL FSQILRASYE FDSPFWDDIS ESAKDFIRHL 250
    LERDPQKRFT CQQALQHLWI SGDAALDRDI LGSVSEQIQK NFARTHWKRA 300
    FNATSFLRHI RKLGQSPEGE EASRQGMTRH SHPGLGTSQS PKW 343
    Length:343
    Mass (Da):38,439
    Last modified:August 1, 1998 - v1
    Checksum:i994D451D809A9D80
    GO
    Isoform 2 (identifier: O70150-2) [UniParc]FASTAAdd to Basket

    Also known as: CamKIbeta1

    The sequence of this isoform differs from the canonical sequence as follows:
         319-343: GEEASRQGMTRHSHPGLGTSQSPKW → ALLISFPDPCPPDWPLLESPRPWV

    Show »
    Length:342
    Mass (Da):38,463
    Checksum:i546C464913E784A2
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei319 – 34325GEEAS…QSPKW → ALLISFPDPCPPDWPLLESP RPWV in isoform 2. 1 PublicationVSP_012636Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D86556 mRNA. Translation: BAA19879.1.
    AB004267 mRNA. Translation: BAA28263.1.
    RefSeqiNP_058971.1. NM_017275.1. [O70150-1]
    XP_006229635.1. XM_006229573.1. [O70150-1]
    XP_006229636.1. XM_006229574.1. [O70150-1]
    XP_006229637.1. XM_006229575.1. [O70150-1]
    XP_006229638.1. XM_006229576.1. [O70150-1]
    UniGeneiRn.11178.

    Genome annotation databases

    EnsembliENSRNOT00000024227; ENSRNOP00000024227; ENSRNOG00000017923. [O70150-1]
    GeneIDi29660.
    KEGGirno:29660.
    UCSCiRGD:69249. rat. [O70150-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D86556 mRNA. Translation: BAA19879.1 .
    AB004267 mRNA. Translation: BAA28263.1 .
    RefSeqi NP_058971.1. NM_017275.1. [O70150-1 ]
    XP_006229635.1. XM_006229573.1. [O70150-1 ]
    XP_006229636.1. XM_006229574.1. [O70150-1 ]
    XP_006229637.1. XM_006229575.1. [O70150-1 ]
    XP_006229638.1. XM_006229576.1. [O70150-1 ]
    UniGenei Rn.11178.

    3D structure databases

    ProteinModelPortali O70150.
    SMRi O70150. Positions 6-310.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 248282. 1 interaction.

    PTM databases

    PhosphoSitei O70150.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000024227 ; ENSRNOP00000024227 ; ENSRNOG00000017923 . [O70150-1 ]
    GeneIDi 29660.
    KEGGi rno:29660.
    UCSCi RGD:69249. rat. [O70150-1 ]

    Organism-specific databases

    CTDi 139728.
    RGDi 69249. Pnck.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00740000115341.
    HOGENOMi HOG000233016.
    HOVERGENi HBG108055.
    InParanoidi O70150.
    KOi K08795.
    OMAi PQKRFTC.
    OrthoDBi EOG7WHH9K.
    PhylomeDBi O70150.
    TreeFami TF314166.

    Miscellaneous databases

    NextBioi 609959.
    PROi O70150.

    Gene expression databases

    Genevestigatori O70150.

    Family and domain databases

    InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    PANTHERi PTHR24347. PTHR24347. 1 hit.
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and comparison of rat cDNAs encoding Ca2+/calmodulin-dependent protein kinase I isoforms."
      Yokokura H., Terada O., Naito Y., Hidaka H.
      Biochim. Biophys. Acta 1338:8-12(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Tissue: Brain.
    2. "Isoform-specific activation and structure diversity of calmodulin kinase I."
      Naito Y., Watanabe Y., Yokokura H., Sugita R., Nishio M., Hidaka H.
      J. Biol. Chem. 272:32704-32708(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY.
      Tissue: Brain.

    Entry informationi

    Entry nameiKCC1B_RAT
    AccessioniPrimary (citable) accession number: O70150
    Secondary accession number(s): O08767
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 2005
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3