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O70150

- KCC1B_RAT

UniProt

O70150 - KCC1B_RAT

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Protein
Calcium/calmodulin-dependent protein kinase type 1B
Gene
Pnck
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro, isoform 1 and isoform 2 phosphorylate CREB1, SYN1/synapsin I. Phosphorylates and activates CAMK1.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by Ca2+/calmodulin. Must be phosphorylated to be maximally active. Activated by CAMKK1.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei44 – 441ATP By similarity
Active sitei136 – 1361Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi21 – 299ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. calmodulin-dependent protein kinase activity Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Calmodulin-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium/calmodulin-dependent protein kinase type 1B (EC:2.7.11.17)
Alternative name(s):
CaM kinase I beta
Short name:
CaM kinase IB
Short name:
CaM-KI beta
Short name:
CaMKI-beta
Pregnancy up-regulated non-ubiquitously-expressed CaM kinase homolog
Gene namesi
Name:Pnck
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 1

Organism-specific databases

RGDi69249. Pnck.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity

GO - Cellular componenti

  1. calcium- and calmodulin-dependent protein kinase complex Source: UniProtKB
  2. cytoplasm Source: UniProtKB-SubCell
  3. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 343343Calcium/calmodulin-dependent protein kinase type 1B
PRO_0000086081Add
BLAST

Post-translational modificationi

Isoform 1 and isoform 2 are phosphorylated by CAMKK1 Inferred.

PTM databases

PhosphoSiteiO70150.

Expressioni

Tissue specificityi

Isoform 1 is expressed in liver, heart, lung, kidney, spleen and testis. Isoform 2 is predominantly expressed in cerebrum and cerebellum.1 Publication

Gene expression databases

GenevestigatoriO70150.

Interactioni

Protein-protein interaction databases

BioGridi248282. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliO70150.
SMRiO70150. Positions 6-310.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 270256Protein kinase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni290 – 31122Calmodulin-binding By similarity
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00740000115341.
HOGENOMiHOG000233016.
HOVERGENiHBG108055.
InParanoidiO70150.
KOiK08795.
OMAiPQKRFTC.
OrthoDBiEOG7WHH9K.
PhylomeDBiO70150.
TreeFamiTF314166.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O70150-1) [UniParc]FASTAAdd to Basket

Also known as: CamKIbeta2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MLLLKKQTED ISSVYEIREK LGSGAFSEVM LAQERGSAHL VALKCIPKKA    50
LRGKEALVEN EIAVLRRISH PNIVALEDVH ESPSHLYLAM ELVTGGELFD 100
RIMERGSYTE KDASHLVGQV LGAVSYLHSL GIVHRDLKPE NLLYATPFED 150
SKIMVSDFGL SKIQAGNMLG TACGTPGYVA PELLEQKPYG KAVDVWALGV 200
ISYILLCGYP PFYDESDPEL FSQILRASYE FDSPFWDDIS ESAKDFIRHL 250
LERDPQKRFT CQQALQHLWI SGDAALDRDI LGSVSEQIQK NFARTHWKRA 300
FNATSFLRHI RKLGQSPEGE EASRQGMTRH SHPGLGTSQS PKW 343
Length:343
Mass (Da):38,439
Last modified:August 1, 1998 - v1
Checksum:i994D451D809A9D80
GO
Isoform 2 (identifier: O70150-2) [UniParc]FASTAAdd to Basket

Also known as: CamKIbeta1

The sequence of this isoform differs from the canonical sequence as follows:
     319-343: GEEASRQGMTRHSHPGLGTSQSPKW → ALLISFPDPCPPDWPLLESPRPWV

Show »
Length:342
Mass (Da):38,463
Checksum:i546C464913E784A2
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei319 – 34325GEEAS…QSPKW → ALLISFPDPCPPDWPLLESP RPWV in isoform 2.
VSP_012636Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D86556 mRNA. Translation: BAA19879.1.
AB004267 mRNA. Translation: BAA28263.1.
RefSeqiNP_058971.1. NM_017275.1. [O70150-1]
XP_006229635.1. XM_006229573.1. [O70150-1]
XP_006229636.1. XM_006229574.1. [O70150-1]
XP_006229637.1. XM_006229575.1. [O70150-1]
XP_006229638.1. XM_006229576.1. [O70150-1]
UniGeneiRn.11178.

Genome annotation databases

EnsembliENSRNOT00000024227; ENSRNOP00000024227; ENSRNOG00000017923. [O70150-1]
GeneIDi29660.
KEGGirno:29660.
UCSCiRGD:69249. rat. [O70150-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D86556 mRNA. Translation: BAA19879.1 .
AB004267 mRNA. Translation: BAA28263.1 .
RefSeqi NP_058971.1. NM_017275.1. [O70150-1 ]
XP_006229635.1. XM_006229573.1. [O70150-1 ]
XP_006229636.1. XM_006229574.1. [O70150-1 ]
XP_006229637.1. XM_006229575.1. [O70150-1 ]
XP_006229638.1. XM_006229576.1. [O70150-1 ]
UniGenei Rn.11178.

3D structure databases

ProteinModelPortali O70150.
SMRi O70150. Positions 6-310.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 248282. 1 interaction.

PTM databases

PhosphoSitei O70150.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000024227 ; ENSRNOP00000024227 ; ENSRNOG00000017923 . [O70150-1 ]
GeneIDi 29660.
KEGGi rno:29660.
UCSCi RGD:69249. rat. [O70150-1 ]

Organism-specific databases

CTDi 139728.
RGDi 69249. Pnck.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00740000115341.
HOGENOMi HOG000233016.
HOVERGENi HBG108055.
InParanoidi O70150.
KOi K08795.
OMAi PQKRFTC.
OrthoDBi EOG7WHH9K.
PhylomeDBi O70150.
TreeFami TF314166.

Miscellaneous databases

NextBioi 609959.
PROi O70150.

Gene expression databases

Genevestigatori O70150.

Family and domain databases

InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
PANTHERi PTHR24347. PTHR24347. 1 hit.
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isolation and comparison of rat cDNAs encoding Ca2+/calmodulin-dependent protein kinase I isoforms."
    Yokokura H., Terada O., Naito Y., Hidaka H.
    Biochim. Biophys. Acta 1338:8-12(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Brain.
  2. "Isoform-specific activation and structure diversity of calmodulin kinase I."
    Naito Y., Watanabe Y., Yokokura H., Sugita R., Nishio M., Hidaka H.
    J. Biol. Chem. 272:32704-32708(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY.
    Tissue: Brain.

Entry informationi

Entry nameiKCC1B_RAT
AccessioniPrimary (citable) accession number: O70150
Secondary accession number(s): O08767
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: August 1, 1998
Last modified: May 14, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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