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Protein

Dual specificity testis-specific protein kinase 1

Gene

Tesk1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues. Probably plays a central role at and after the meiotic phase of spermatogenesis (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

Activated by autophosphorylation on Ser-215. Kinase activity is inhibited by SPRED1 (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei81 – 811ATPPROSITE-ProRule annotation
Active sitei170 – 1701Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi58 – 669ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity testis-specific protein kinase 1 (EC:2.7.12.1)
Alternative name(s):
Testicular protein kinase 1
Gene namesi
Name:Tesk1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1201675. Tesk1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 627627Dual specificity testis-specific protein kinase 1PRO_0000086747Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei215 – 2151Phosphoserine; by autocatalysisBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO70146.
PaxDbiO70146.
PRIDEiO70146.

PTM databases

iPTMnetiO70146.
PhosphoSiteiO70146.

Expressioni

Tissue specificityi

Predominantly expressed in testis.1 Publication

Gene expression databases

BgeeiO70146.
CleanExiMM_TESK1.
ExpressionAtlasiO70146. baseline and differential.
GenevisibleiO70146. MM.

Interactioni

Subunit structurei

Interacts with SPRY4. Interacts with TAOK2; leading to inhibit TAOK2 activity. Interacts with SPRED1; leading to inhibit activity (By similarity).By similarity

Protein-protein interaction databases

BioGridi204120. 1 interaction.
STRINGi10090.ENSMUSP00000050087.

Structurei

3D structure databases

ProteinModelPortaliO70146.
SMRiO70146. Positions 48-322.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini52 – 309258Protein kinasePROSITE-ProRule annotationAdd
BLAST

Domaini

The extracatalytic C-terminal part is highly rich in proline residues.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IF1S. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00530000063025.
HOGENOMiHOG000231415.
HOVERGENiHBG058204.
InParanoidiO70146.
KOiK08841.
OMAiKMECEGS.
OrthoDBiEOG7V7664.
PhylomeDBiO70146.
TreeFamiTF318014.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR015782. TESK1.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PANTHERiPTHR23257:SF383. PTHR23257:SF383. 1 hit.
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O70146-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGERPPLRG PGPGEAPGEG PGGAGGGPGR GRPSSYRALR SAVSSLARVD
60 70 80 90 100
DFDCAEKIGA GFFSEVYKVR HRQSGQVMVL KMNKLPSNRS NTLREVQLMN
110 120 130 140 150
RLRHPNILRF MGVCVHQGQL HALTEYMNGG TLEQLLSSPE PLSWPVRLHL
160 170 180 190 200
ALDIAQGLRY LHAKGVFHRD LTSKNCLVRR EDRGFTAVVG DFGLAEKIPV
210 220 230 240 250
YREGTRKEPL AVVGSPYWMA PEVLRGELYD EKADVFAFGI VLCELIARVP
260 270 280 290 300
ADPDYLPRTE DFGLDVPAFR TLVGNDCPLP FLLLAIHCCS MEPSTRAPFT
310 320 330 340 350
EITQHLEQIL EQQPEATPLA KPPLTKAPLT YNQGSVPRGG PSATLPRPDP
360 370 380 390 400
RLSRSRSDLF LPPSPESPPS WGDNLTRVNP FSLREDLRGG KIKLLDTPCK
410 420 430 440 450
PATPLPLVPP SPLTSTQLPL VTTPDILVQP ETPVRRCRSL PSSPELPRRM
460 470 480 490 500
ETALPGPGPS PMGPTEERMD CEGSSPEPEP PGLAPQLPLA VATDNFISTC
510 520 530 540 550
SSASQPWSPR SGPPLNNNPP AVVVNSPQGW AREPWNRAQH SLPRAAALER
560 570 580 590 600
TEPSPPPSAP REPEEGLPCP GCCLGPFSFG FLSMCPRPTP AVARYRNLNC
610 620
EAGSLLCHRG HHAKPPTPSL QLPGARS
Length:627
Mass (Da):68,052
Last modified:October 23, 2007 - v3
Checksum:i091ADA8CF52A2B50
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti550 – 5501R → Q in BAA25124 (PubMed:9469938).Curated
Sequence conflicti550 – 5501R → Q in BAA25125 (PubMed:9469938).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003493 Genomic DNA. Translation: BAA25124.1.
AB003494 mRNA. Translation: BAA25125.1.
AK144302 mRNA. Translation: BAE25822.1.
AL732506 Genomic DNA. Translation: CAM17173.1.
BC099699 mRNA. Translation: AAH99699.1.
CCDSiCCDS18094.1.
PIRiJC6534.
RefSeqiNP_035701.3. NM_011571.3.
UniGeneiMm.485441.

Genome annotation databases

EnsembliENSMUST00000060864; ENSMUSP00000050087; ENSMUSG00000028458.
GeneIDi21754.
KEGGimmu:21754.
UCSCiuc008spq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003493 Genomic DNA. Translation: BAA25124.1.
AB003494 mRNA. Translation: BAA25125.1.
AK144302 mRNA. Translation: BAE25822.1.
AL732506 Genomic DNA. Translation: CAM17173.1.
BC099699 mRNA. Translation: AAH99699.1.
CCDSiCCDS18094.1.
PIRiJC6534.
RefSeqiNP_035701.3. NM_011571.3.
UniGeneiMm.485441.

3D structure databases

ProteinModelPortaliO70146.
SMRiO70146. Positions 48-322.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204120. 1 interaction.
STRINGi10090.ENSMUSP00000050087.

PTM databases

iPTMnetiO70146.
PhosphoSiteiO70146.

Proteomic databases

MaxQBiO70146.
PaxDbiO70146.
PRIDEiO70146.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000060864; ENSMUSP00000050087; ENSMUSG00000028458.
GeneIDi21754.
KEGGimmu:21754.
UCSCiuc008spq.2. mouse.

Organism-specific databases

CTDi7016.
MGIiMGI:1201675. Tesk1.

Phylogenomic databases

eggNOGiENOG410IF1S. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00530000063025.
HOGENOMiHOG000231415.
HOVERGENiHBG058204.
InParanoidiO70146.
KOiK08841.
OMAiKMECEGS.
OrthoDBiEOG7V7664.
PhylomeDBiO70146.
TreeFamiTF318014.

Enzyme and pathway databases

BRENDAi2.7.10.2. 3474.

Miscellaneous databases

PROiO70146.
SOURCEiSearch...

Gene expression databases

BgeeiO70146.
CleanExiMM_TESK1.
ExpressionAtlasiO70146. baseline and differential.
GenevisibleiO70146. MM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR015782. TESK1.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PANTHERiPTHR23257:SF383. PTHR23257:SF383. 1 hit.
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural organization and chromosomal localization of the mouse tesk1 (testis-specific protein kinase 1) gene."
    Toshima J., Nakagawara K., Mori M., Noda T., Mizuno K.
    Gene 206:237-245(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY.
    Strain: 129/Sv.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Testis.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.

Entry informationi

Entry nameiTESK1_MOUSE
AccessioniPrimary (citable) accession number: O70146
Secondary accession number(s): O70147, Q499W7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: October 23, 2007
Last modified: June 8, 2016
This is version 143 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.