Reviewed, UniProtKB/Swiss-Prot O70146 (TESK1_MOUSE)

Names and origin

Protein names

Recommended name:
    Dual specificity testis-specific protein kinase 1
    EC=2.7.12.1
Alternative name(s):
    Testicular protein kinase 1

Gene names

Name:

Tesk1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	627 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues. Probably plays a central role at and after the meiotic phase of spermatogenesis By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity. Manganese By similarity.
Enzyme regulation	Activated by autophosphorylation on Ser-215 By similarity.
Subunit structure	Interacts with SPRY4 By similarity.
Tissue specificity	Predominantly expressed in testis. Ref.1
Domain	The extracatalytic C-terminal part is highly rich in proline residues.
Sequence similarities	Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 627	627	Dual specificity testis-specific protein kinase 1		PRO_0000086747
Regions
	Domain	52 – 309	258	Protein kinase
	Nucleotide binding	58 – 66	9	ATP By similarity
Sites
	Active site	170	1	Proton acceptor By similarity
	Binding site	81	1	ATP By similarity
Amino acid modifications
	Modified residue	215	1	Phosphoserine; by autocatalysis By similarity
Experimental info
	Sequence conflict	550	1	R → Q in BAA25124. Ref.1
	Sequence conflict	550	1	R → Q in BAA25125. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O70146-1 [UniParc]. Last modified October 23, 2007. Version 3. Checksum: 091ADA8CF52A2B50	FASTA	627	68,052
	10 20 30 40 50 60 MAGERPPLRG PGPGEAPGEG PGGAGGGPGR GRPSSYRALR SAVSSLARVD DFDCAEKIGA 70 80 90 100 110 120 GFFSEVYKVR HRQSGQVMVL KMNKLPSNRS NTLREVQLMN RLRHPNILRF MGVCVHQGQL 130 140 150 160 170 180 HALTEYMNGG TLEQLLSSPE PLSWPVRLHL ALDIAQGLRY LHAKGVFHRD LTSKNCLVRR 190 200 210 220 230 240 EDRGFTAVVG DFGLAEKIPV YREGTRKEPL AVVGSPYWMA PEVLRGELYD EKADVFAFGI 250 260 270 280 290 300 VLCELIARVP ADPDYLPRTE DFGLDVPAFR TLVGNDCPLP FLLLAIHCCS MEPSTRAPFT 310 320 330 340 350 360 EITQHLEQIL EQQPEATPLA KPPLTKAPLT YNQGSVPRGG PSATLPRPDP RLSRSRSDLF 370 380 390 400 410 420 LPPSPESPPS WGDNLTRVNP FSLREDLRGG KIKLLDTPCK PATPLPLVPP SPLTSTQLPL 430 440 450 460 470 480 VTTPDILVQP ETPVRRCRSL PSSPELPRRM ETALPGPGPS PMGPTEERMD CEGSSPEPEP 490 500 510 520 530 540 PGLAPQLPLA VATDNFISTC SSASQPWSPR SGPPLNNNPP AVVVNSPQGW AREPWNRAQH 550 560 570 580 590 600 SLPRAAALER TEPSPPPSAP REPEEGLPCP GCCLGPFSFG FLSMCPRPTP AVARYRNLNC 610 620 EAGSLLCHRG HHAKPPTPSL QLPGARS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural organization and chromosomal localization of the mouse tesk1 (testis-specific protein kinase 1) gene." Toshima J., Nakagawara K., Mori M., Noda T., Mizuno K. Gene 206:237-245(1998) [PubMed: 9469938] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY. Strain: 129/Sv.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Testis.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB003493 Genomic DNA. Translation: BAA25124.1. AB003494 mRNA. Translation: BAA25125.1. AK144302 mRNA. Translation: BAE25822.1. AL732506 Genomic DNA. Translation: CAM17173.1. BC099699 mRNA. Translation: AAH99699.1.
IPI	IPI00316791.
PIR	JC6534.
RefSeq	NP_035701.3.
UniGene	Mm.471656
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O70146.
PTM databases
PhosphoSite	O70146.
Proteomic databases
PRIDE	O70146.
Genome annotation databases
Ensembl	ENSMUST00000060864; ENSMUSP00000050087; ENSMUSG00000028458; Mus musculus. [Genome view]
GeneID	21754.
KEGG	mmu:21754.
NMPDR	fig|10090.3.peg.9470.
UCSC	uc008spq.1. mouse.
Organism-specific databases
CTD	21754.
MGI	MGI:1201675. Tesk1.
Phylogenomic databases
HOGENOM	O70146.
HOVERGEN	O70146.
OMA	DNFISTC.
Enzyme and pathway databases
BRENDA	2.7.10.2. 244. 2.7.12.1. 244.
Gene expression databases
ArrayExpress	O70146.
Bgee	O70146.
CleanEx	MM_TESK1.
Genevestigator	O70146.
GermOnline	ENSMUSG00000028458. Mus musculus.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR015782. Testis-specific_kinase_1. IPR001245. Tyr_pkinase. IPR008266. Tyr_pkinase_AS. [Graphical view]
PANTHER	PTHR23257:SF10. TESK1. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR00109. TYRKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	301059.
SOURCE	Search...

Entry information

Entry name

TESK1_MOUSE

Accession

Primary (citable) accession number: O70146
Secondary accession number(s): O70147, Q499W7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	October 23, 2007
Last modified:	November 3, 2009
This is version 91 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)