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Protein

Neutrophil cytosol factor 2

Gene

Ncf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-1222556. ROS, RNS production in response to bacteria.
R-MMU-1236973. Cross-presentation of particulate exogenous antigens (phagosomes).
R-MMU-4420097. VEGFA-VEGFR2 Pathway.
R-MMU-5668599. RHO GTPases Activate NADPH Oxidases.

Names & Taxonomyi

Protein namesi
Recommended name:
Neutrophil cytosol factor 2
Short name:
NCF-2
Alternative name(s):
67 kDa neutrophil oxidase factor
NADPH oxidase activator 2
Neutrophil NADPH oxidase factor 2
p67-phox
Gene namesi
Name:Ncf2Imported
Synonyms:Noxa2, P67phox
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:97284. Ncf2.

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

  • acrosomal vesicle Source: MGI
  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • nucleolus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003122271 – 525Neutrophil cytosol factor 2Add BLAST525

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei233PhosphothreonineCombined sources1
Modified residuei324PhosphoserineCombined sources1
Modified residuei398PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO70145.
PaxDbiO70145.
PeptideAtlasiO70145.
PRIDEiO70145.

PTM databases

iPTMnetiO70145.
PhosphoSitePlusiO70145.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026480.
CleanExiMM_NCF2.
ExpressionAtlasiO70145. baseline and differential.
GenevisibleiO70145. MM.

Interactioni

Subunit structurei

Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF4. Interacts (via the C-terminal SH3 domain) with NCF1 (via C-terminus). Interacts with SYTL1 and RAC1. May interact with NOXO1. Interacts with S100A8 and calprotectin (S100A8/9) (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Prdx6O087093EBI-9550667,EBI-444895

GO - Molecular functioni

  • protein C-terminus binding Source: MGI
  • Rac GTPase binding Source: MGI

Protein-protein interaction databases

IntActiO70145. 2 interactors.
STRINGi10090.ENSMUSP00000027754.

Structurei

3D structure databases

ProteinModelPortaliO70145.
SMRiO70145.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati37 – 70TPR 1Sequence analysisAdd BLAST34
Repeati71 – 104TPR 2Sequence analysisAdd BLAST34
Repeati121 – 154TPR 3Sequence analysisAdd BLAST34
Domaini240 – 299SH3 1PROSITE-ProRule annotationAdd BLAST60
Domaini350 – 428PB1PROSITE-ProRule annotationAdd BLAST79
Domaini456 – 515SH3 2PROSITE-ProRule annotationAdd BLAST60

Domaini

The OPR/PB1 domain mediates the association with NCF4/p40-PHOX.By similarity

Sequence similaritiesi

Belongs to the NCF2/NOXA1 family.Curated
Contains 1 PB1 domain.PROSITE-ProRule annotation
Contains 2 SH3 domains.PROSITE-ProRule annotation
Contains 3 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3 domain, TPR repeat

Phylogenomic databases

eggNOGiKOG4225. Eukaryota.
ENOG41110AD. LUCA.
GeneTreeiENSGT00530000063843.
HOGENOMiHOG000237312.
HOVERGENiHBG001521.
InParanoidiO70145.
KOiK08010.
OMAiYSQVRDM.
OrthoDBiEOG091G04T7.
PhylomeDBiO70145.
TreeFamiTF329087.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR000270. PB1_dom.
IPR001452. SH3_domain.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF00564. PB1. 1 hit.
PF00018. SH3_1. 2 hits.
PF00515. TPR_1. 1 hit.
PF13181. TPR_8. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00666. PB1. 1 hit.
SM00326. SH3. 2 hits.
SM00028. TPR. 3 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
SSF50044. SSF50044. 2 hits.
PROSITEiPS51745. PB1. 1 hit.
PS50002. SH3. 2 hits.
PS50005. TPR. 3 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O70145-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLAEAIRLW NEGVLAADKK DWKGALEAFS EVQDPHSRIC FNIGCVNTIL
60 70 80 90 100
ENLQAAEQAF TKSINRDKHS AVAYFQRGML YYRMEKYDLA IKDLKEALTQ
110 120 130 140 150
LRGNQLIDYK ILGLQFKLFA CEVLYNIALM HAKKEEWKKA EEQLALATNM
160 170 180 190 200
KSEPRHSKID KAMESIWKQK LFEPVVIPVG RLFRPNERQV AQLAKKDYLG
210 220 230 240 250
KATVVASVVH QDNFSGFAPL QPQSAEPPPR PKTPEIFRAL EGEAHRVLFG
260 270 280 290 300
FVPETPEELQ VMPGNIVFVL KKGSDNWATV MFNGQKGLVP CNYLEPVELR
310 320 330 340 350
IHPQSQPQED TSPESDIPPP PNSSPPGRLQ LSPGHKQKEP KELKLSVPMP
360 370 380 390 400
YMLKVHYKYT VVMETRLGLP YSQLRNMVSK KLALSPEHTK LSYRRRDSHE
410 420 430 440 450
LLLLSEESMK DAWGQVKNYC LTLWCEHTVG DQGLIDEPIQ RENSDASKQT
460 470 480 490 500
TEPQPKEGTQ VVAIFSYEAA QPEDLEFVEG DVILVLSHVN EEWLEGECKG
510 520
KVGIFPKAFV EGCAAKNLEG IPREV
Length:525
Mass (Da):59,485
Last modified:August 1, 1998 - v1
Checksum:i663DB6B52D790F76
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31E → A in BAE42353 (PubMed:16141072).Curated1
Sequence conflicti31E → A in BAE42218 (PubMed:16141072).Curated1
Sequence conflicti31E → A in BAE42065 (PubMed:16141072).Curated1
Sequence conflicti31E → A in BAE33191 (PubMed:16141072).Curated1
Sequence conflicti324S → N in BAE32002 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB002664 mRNA. Translation: BAA25650.1.
AK036379 mRNA. Translation: BAC29404.1.
AK137152 mRNA. Translation: BAE23254.1.
AK153447 mRNA. Translation: BAE32002.1.
AK155324 mRNA. Translation: BAE33191.1.
AK170839 mRNA. Translation: BAE42065.1.
AK171059 mRNA. Translation: BAE42218.1.
AK171265 mRNA. Translation: BAE42353.1.
BC003730 mRNA. Translation: AAH03730.1.
CCDSiCCDS15367.1.
RefSeqiNP_035007.1. NM_010877.5.
XP_006529299.1. XM_006529236.3.
UniGeneiMm.270307.
Mm.489200.

Genome annotation databases

EnsembliENSMUST00000027754; ENSMUSP00000027754; ENSMUSG00000026480.
ENSMUST00000186568; ENSMUSP00000140404; ENSMUSG00000026480.
GeneIDi17970.
KEGGimmu:17970.
UCSCiuc007czm.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB002664 mRNA. Translation: BAA25650.1.
AK036379 mRNA. Translation: BAC29404.1.
AK137152 mRNA. Translation: BAE23254.1.
AK153447 mRNA. Translation: BAE32002.1.
AK155324 mRNA. Translation: BAE33191.1.
AK170839 mRNA. Translation: BAE42065.1.
AK171059 mRNA. Translation: BAE42218.1.
AK171265 mRNA. Translation: BAE42353.1.
BC003730 mRNA. Translation: AAH03730.1.
CCDSiCCDS15367.1.
RefSeqiNP_035007.1. NM_010877.5.
XP_006529299.1. XM_006529236.3.
UniGeneiMm.270307.
Mm.489200.

3D structure databases

ProteinModelPortaliO70145.
SMRiO70145.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO70145. 2 interactors.
STRINGi10090.ENSMUSP00000027754.

PTM databases

iPTMnetiO70145.
PhosphoSitePlusiO70145.

Proteomic databases

MaxQBiO70145.
PaxDbiO70145.
PeptideAtlasiO70145.
PRIDEiO70145.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027754; ENSMUSP00000027754; ENSMUSG00000026480.
ENSMUST00000186568; ENSMUSP00000140404; ENSMUSG00000026480.
GeneIDi17970.
KEGGimmu:17970.
UCSCiuc007czm.1. mouse.

Organism-specific databases

CTDi4688.
MGIiMGI:97284. Ncf2.

Phylogenomic databases

eggNOGiKOG4225. Eukaryota.
ENOG41110AD. LUCA.
GeneTreeiENSGT00530000063843.
HOGENOMiHOG000237312.
HOVERGENiHBG001521.
InParanoidiO70145.
KOiK08010.
OMAiYSQVRDM.
OrthoDBiEOG091G04T7.
PhylomeDBiO70145.
TreeFamiTF329087.

Enzyme and pathway databases

ReactomeiR-MMU-1222556. ROS, RNS production in response to bacteria.
R-MMU-1236973. Cross-presentation of particulate exogenous antigens (phagosomes).
R-MMU-4420097. VEGFA-VEGFR2 Pathway.
R-MMU-5668599. RHO GTPases Activate NADPH Oxidases.

Miscellaneous databases

PROiO70145.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026480.
CleanExiMM_NCF2.
ExpressionAtlasiO70145. baseline and differential.
GenevisibleiO70145. MM.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR000270. PB1_dom.
IPR001452. SH3_domain.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF00564. PB1. 1 hit.
PF00018. SH3_1. 2 hits.
PF00515. TPR_1. 1 hit.
PF13181. TPR_8. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00666. PB1. 1 hit.
SM00326. SH3. 2 hits.
SM00028. TPR. 3 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
SSF50044. SSF50044. 2 hits.
PROSITEiPS51745. PB1. 1 hit.
PS50002. SH3. 2 hits.
PS50005. TPR. 3 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNCF2_MOUSE
AccessioniPrimary (citable) accession number: O70145
Secondary accession number(s): Q3TC92, Q3U5S4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: August 1, 1998
Last modified: November 30, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.