Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O70145 (NCF2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neutrophil cytosol factor 2

Short name=NCF-2
Alternative name(s):
67 kDa neutrophil oxidase factor
NADPH oxidase activator 2
Neutrophil NADPH oxidase factor 2
p67-phox
Gene names
Name:Ncf2
Synonyms:Noxa2, P67phox
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length525 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production) By similarity. UniProtKB P19878

Subunit structure

Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF4. Interacts (via the C-terminal SH3 domain) with NCF1 (via C-terminus). Interacts with SYTL1 and RAC1. May interact with NOXO1. Interacts with S100A8 and calprotectin (S100A8/9) By similarity.

Subcellular location

Cytoplasm By similarity UniProtKB P19878.

Domain

The OPR/PB1 domain mediates the association with NCF4/p40-PHOX By similarity.

Sequence similarities

Belongs to the NCF2/NOXA1 family.

Contains 1 OPR domain.

Contains 2 SH3 domains.

Contains 3 TPR repeats.

Ontologies

Keywords
   Cellular componentCytoplasm
   DomainRepeat
SH3 domain
TPR repeat
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processNADP catabolic process

Inferred from sequence alignment PubMed 10647999. Source: MGI

aging

Inferred from electronic annotation. Source: Ensembl

cellular response to hormone stimulus

Inferred from electronic annotation. Source: Ensembl

oxidation-reduction process

Inferred from direct assay Ref.1. Source: GOC

positive regulation of blood pressure

Inferred from electronic annotation. Source: Ensembl

positive regulation of neuron apoptotic process

Inferred from electronic annotation. Source: Ensembl

response to activity

Inferred from electronic annotation. Source: Ensembl

response to drug

Inferred from electronic annotation. Source: Ensembl

response to glucose

Inferred from electronic annotation. Source: Ensembl

response to hyperoxia

Inferred from electronic annotation. Source: Ensembl

response to laminar fluid shear stress

Inferred from electronic annotation. Source: Ensembl

response to lipopolysaccharide

Inferred from electronic annotation. Source: Ensembl

response to progesterone

Inferred from electronic annotation. Source: Ensembl

superoxide anion generation

Inferred from direct assay Ref.1. Source: MGI

superoxide metabolic process

Inferred from sequence alignment PubMed 10647999. Source: MGI

   Cellular_componentacrosomal vesicle

Inferred from direct assay PubMed 15850784. Source: MGI

cytoplasm

Inferred from direct assay PubMed 15850784. Source: MGI

cytosol

Inferred from sequence alignment PubMed 10647999. Source: MGI

membrane

Inferred from electronic annotation. Source: Ensembl

nucleolus

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionRac GTPase binding

Inferred from physical interaction Ref.1. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 525525Neutrophil cytosol factor 2
PRO_0000312227

Regions

Repeat37 – 7034TPR 1
Repeat71 – 10434TPR 2
Repeat121 – 15434TPR 3
Domain240 – 29960SH3 1
Domain350 – 42879OPR
Domain456 – 51560SH3 2

Experimental info

Sequence conflict311E → A in BAE42353. Ref.2
Sequence conflict311E → A in BAE42218. Ref.2
Sequence conflict311E → A in BAE42065. Ref.2
Sequence conflict311E → A in BAE33191. Ref.2
Sequence conflict3241S → N in BAE32002. Ref.2

Sequences

Sequence LengthMass (Da)Tools
O70145 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 663DB6B52D790F76

FASTA52559,485
        10         20         30         40         50         60 
MSLAEAIRLW NEGVLAADKK DWKGALEAFS EVQDPHSRIC FNIGCVNTIL ENLQAAEQAF 

        70         80         90        100        110        120 
TKSINRDKHS AVAYFQRGML YYRMEKYDLA IKDLKEALTQ LRGNQLIDYK ILGLQFKLFA 

       130        140        150        160        170        180 
CEVLYNIALM HAKKEEWKKA EEQLALATNM KSEPRHSKID KAMESIWKQK LFEPVVIPVG 

       190        200        210        220        230        240 
RLFRPNERQV AQLAKKDYLG KATVVASVVH QDNFSGFAPL QPQSAEPPPR PKTPEIFRAL 

       250        260        270        280        290        300 
EGEAHRVLFG FVPETPEELQ VMPGNIVFVL KKGSDNWATV MFNGQKGLVP CNYLEPVELR 

       310        320        330        340        350        360 
IHPQSQPQED TSPESDIPPP PNSSPPGRLQ LSPGHKQKEP KELKLSVPMP YMLKVHYKYT 

       370        380        390        400        410        420 
VVMETRLGLP YSQLRNMVSK KLALSPEHTK LSYRRRDSHE LLLLSEESMK DAWGQVKNYC 

       430        440        450        460        470        480 
LTLWCEHTVG DQGLIDEPIQ RENSDASKQT TEPQPKEGTQ VVAIFSYEAA QPEDLEFVEG 

       490        500        510        520 
DVILVLSHVN EEWLEGECKG KVGIFPKAFV EGCAAKNLEG IPREV 

« Hide

References

« Hide 'large scale' references
[1]"Functional modules and expression of mouse p40(phox) and p67(phox), SH3-domain-containing proteins involved in the phagocyte NADPH oxidase complex."
Mizuki K., Kadomatsu K., Hata K., Ito T., Fan Q.-W., Kage Y., Fukumaki Y., Sakaki Y., Takeshige K., Sumimoto H.
Eur. J. Biochem. 251:573-582(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Myelomonocyte.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and NOD.
Tissue: Bone, Dendritic cell, Macrophage and Urinary bladder.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary gland.
[4]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB002664 mRNA. Translation: BAA25650.1.
AK036379 mRNA. Translation: BAC29404.1.
AK137152 mRNA. Translation: BAE23254.1.
AK153447 mRNA. Translation: BAE32002.1.
AK155324 mRNA. Translation: BAE33191.1.
AK170839 mRNA. Translation: BAE42065.1.
AK171059 mRNA. Translation: BAE42218.1.
AK171265 mRNA. Translation: BAE42353.1.
BC003730 mRNA. Translation: AAH03730.1.
RefSeqNP_035007.1. NM_010877.4.
XP_006529298.1. XM_006529235.1.
XP_006529299.1. XM_006529236.1.
UniGeneMm.270307.

3D structure databases

ProteinModelPortalO70145.
SMRO70145. Positions 2-193, 242-297, 351-426, 454-514.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteO70145.

Proteomic databases

PaxDbO70145.
PRIDEO70145.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000027754; ENSMUSP00000027754; ENSMUSG00000026480.
GeneID17970.
KEGGmmu:17970.
UCSCuc007czm.1. mouse.

Organism-specific databases

CTD4688.
MGIMGI:97284. Ncf2.

Phylogenomic databases

eggNOGNOG248396.
GeneTreeENSGT00530000063843.
HOGENOMHOG000237312.
HOVERGENHBG001521.
InParanoidO70145.
KOK08010.
OMAYSQVRDM.
OrthoDBEOG7G1V62.
PhylomeDBO70145.
TreeFamTF329087.

Gene expression databases

BgeeO70145.
CleanExMM_NCF2.
GenevestigatorO70145.

Family and domain databases

Gene3D1.25.40.10. 1 hit.
InterProIPR000270. OPR_PB1.
IPR001452. SH3_domain.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamPF00564. PB1. 1 hit.
PF00018. SH3_1. 2 hits.
PF00515. TPR_1. 1 hit.
[Graphical view]
PRINTSPR00452. SH3DOMAIN.
SMARTSM00666. PB1. 1 hit.
SM00326. SH3. 2 hits.
SM00028. TPR. 3 hits.
[Graphical view]
SUPFAMSSF50044. SSF50044. 2 hits.
PROSITEPS50002. SH3. 2 hits.
PS50005. TPR. 3 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio292917.
PROO70145.
SOURCESearch...

Entry information

Entry nameNCF2_MOUSE
AccessionPrimary (citable) accession number: O70145
Secondary accession number(s): Q3TC92, Q3U5S4
Entry history
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: August 1, 1998
Last modified: April 16, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot