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O70145

- NCF2_MOUSE

UniProt

O70145 - NCF2_MOUSE

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Protein

Neutrophil cytosol factor 2

Gene

Ncf2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).By similarity

GO - Molecular functioni

  1. Rac GTPase binding Source: MGI

GO - Biological processi

  1. NADP catabolic process Source: MGI
  2. oxidation-reduction process Source: GOC
  3. superoxide anion generation Source: MGI
  4. superoxide metabolic process Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_198345. Phagosomal maturation (early endosomal stage).
REACT_199093. Cross-presentation of particulate exogenous antigens (phagosomes).

Names & Taxonomyi

Protein namesi
Recommended name:
Neutrophil cytosol factor 2
Short name:
NCF-2
Alternative name(s):
67 kDa neutrophil oxidase factor
NADPH oxidase activator 2
Neutrophil NADPH oxidase factor 2
p67-phox
Gene namesi
Name:Ncf2Imported
Synonyms:Noxa2, P67phox
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:97284. Ncf2.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. acrosomal vesicle Source: MGI
  2. cytoplasm Source: MGI
  3. cytosol Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 525525Neutrophil cytosol factor 2PRO_0000312227Add
BLAST

Proteomic databases

MaxQBiO70145.
PaxDbiO70145.
PRIDEiO70145.

PTM databases

PhosphoSiteiO70145.

Expressioni

Gene expression databases

BgeeiO70145.
CleanExiMM_NCF2.
GenevestigatoriO70145.

Interactioni

Subunit structurei

Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF4. Interacts (via the C-terminal SH3 domain) with NCF1 (via C-terminus). Interacts with SYTL1 and RAC1. May interact with NOXO1. Interacts with S100A8 and calprotectin (S100A8/9) (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Prdx6O087093EBI-9550667,EBI-444895

Protein-protein interaction databases

IntActiO70145. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliO70145.
SMRiO70145. Positions 2-193, 242-297, 351-426, 454-514.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati37 – 7034TPR 1Sequence AnalysisAdd
BLAST
Repeati71 – 10434TPR 2Sequence AnalysisAdd
BLAST
Repeati121 – 15434TPR 3Sequence AnalysisAdd
BLAST
Domaini240 – 29960SH3 1PROSITE-ProRule annotationAdd
BLAST
Domaini350 – 42879OPRSequence AnalysisAdd
BLAST
Domaini456 – 51560SH3 2PROSITE-ProRule annotationAdd
BLAST

Domaini

The OPR/PB1 domain mediates the association with NCF4/p40-PHOX.By similarity

Sequence similaritiesi

Belongs to the NCF2/NOXA1 family.Curated
Contains 1 OPR domain.Sequence Analysis
Contains 2 SH3 domains.PROSITE-ProRule annotation
Contains 3 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3 domain, TPR repeat

Phylogenomic databases

eggNOGiNOG248396.
GeneTreeiENSGT00530000063843.
HOGENOMiHOG000237312.
HOVERGENiHBG001521.
InParanoidiO70145.
KOiK08010.
OMAiYSQVRDM.
OrthoDBiEOG7G1V62.
PhylomeDBiO70145.
TreeFamiTF329087.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR000270. OPR_PB1.
IPR001452. SH3_domain.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF00564. PB1. 1 hit.
PF00018. SH3_1. 2 hits.
PF00515. TPR_1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00666. PB1. 1 hit.
SM00326. SH3. 2 hits.
SM00028. TPR. 3 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 2 hits.
PROSITEiPS50002. SH3. 2 hits.
PS50005. TPR. 3 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O70145-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLAEAIRLW NEGVLAADKK DWKGALEAFS EVQDPHSRIC FNIGCVNTIL
60 70 80 90 100
ENLQAAEQAF TKSINRDKHS AVAYFQRGML YYRMEKYDLA IKDLKEALTQ
110 120 130 140 150
LRGNQLIDYK ILGLQFKLFA CEVLYNIALM HAKKEEWKKA EEQLALATNM
160 170 180 190 200
KSEPRHSKID KAMESIWKQK LFEPVVIPVG RLFRPNERQV AQLAKKDYLG
210 220 230 240 250
KATVVASVVH QDNFSGFAPL QPQSAEPPPR PKTPEIFRAL EGEAHRVLFG
260 270 280 290 300
FVPETPEELQ VMPGNIVFVL KKGSDNWATV MFNGQKGLVP CNYLEPVELR
310 320 330 340 350
IHPQSQPQED TSPESDIPPP PNSSPPGRLQ LSPGHKQKEP KELKLSVPMP
360 370 380 390 400
YMLKVHYKYT VVMETRLGLP YSQLRNMVSK KLALSPEHTK LSYRRRDSHE
410 420 430 440 450
LLLLSEESMK DAWGQVKNYC LTLWCEHTVG DQGLIDEPIQ RENSDASKQT
460 470 480 490 500
TEPQPKEGTQ VVAIFSYEAA QPEDLEFVEG DVILVLSHVN EEWLEGECKG
510 520
KVGIFPKAFV EGCAAKNLEG IPREV
Length:525
Mass (Da):59,485
Last modified:August 1, 1998 - v1
Checksum:i663DB6B52D790F76
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti31 – 311E → A in BAE42353. (PubMed:16141072)Curated
Sequence conflicti31 – 311E → A in BAE42218. (PubMed:16141072)Curated
Sequence conflicti31 – 311E → A in BAE42065. (PubMed:16141072)Curated
Sequence conflicti31 – 311E → A in BAE33191. (PubMed:16141072)Curated
Sequence conflicti324 – 3241S → N in BAE32002. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB002664 mRNA. Translation: BAA25650.1.
AK036379 mRNA. Translation: BAC29404.1.
AK137152 mRNA. Translation: BAE23254.1.
AK153447 mRNA. Translation: BAE32002.1.
AK155324 mRNA. Translation: BAE33191.1.
AK170839 mRNA. Translation: BAE42065.1.
AK171059 mRNA. Translation: BAE42218.1.
AK171265 mRNA. Translation: BAE42353.1.
BC003730 mRNA. Translation: AAH03730.1.
CCDSiCCDS15367.1.
RefSeqiNP_035007.1. NM_010877.4.
XP_006529298.1. XM_006529235.1.
XP_006529299.1. XM_006529236.1.
UniGeneiMm.270307.

Genome annotation databases

EnsembliENSMUST00000027754; ENSMUSP00000027754; ENSMUSG00000026480.
ENSMUST00000186568; ENSMUSP00000140404; ENSMUSG00000026480.
GeneIDi17970.
KEGGimmu:17970.
UCSCiuc007czm.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB002664 mRNA. Translation: BAA25650.1 .
AK036379 mRNA. Translation: BAC29404.1 .
AK137152 mRNA. Translation: BAE23254.1 .
AK153447 mRNA. Translation: BAE32002.1 .
AK155324 mRNA. Translation: BAE33191.1 .
AK170839 mRNA. Translation: BAE42065.1 .
AK171059 mRNA. Translation: BAE42218.1 .
AK171265 mRNA. Translation: BAE42353.1 .
BC003730 mRNA. Translation: AAH03730.1 .
CCDSi CCDS15367.1.
RefSeqi NP_035007.1. NM_010877.4.
XP_006529298.1. XM_006529235.1.
XP_006529299.1. XM_006529236.1.
UniGenei Mm.270307.

3D structure databases

ProteinModelPortali O70145.
SMRi O70145. Positions 2-193, 242-297, 351-426, 454-514.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O70145. 2 interactions.

PTM databases

PhosphoSitei O70145.

Proteomic databases

MaxQBi O70145.
PaxDbi O70145.
PRIDEi O70145.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000027754 ; ENSMUSP00000027754 ; ENSMUSG00000026480 .
ENSMUST00000186568 ; ENSMUSP00000140404 ; ENSMUSG00000026480 .
GeneIDi 17970.
KEGGi mmu:17970.
UCSCi uc007czm.1. mouse.

Organism-specific databases

CTDi 4688.
MGIi MGI:97284. Ncf2.

Phylogenomic databases

eggNOGi NOG248396.
GeneTreei ENSGT00530000063843.
HOGENOMi HOG000237312.
HOVERGENi HBG001521.
InParanoidi O70145.
KOi K08010.
OMAi YSQVRDM.
OrthoDBi EOG7G1V62.
PhylomeDBi O70145.
TreeFami TF329087.

Enzyme and pathway databases

Reactomei REACT_198345. Phagosomal maturation (early endosomal stage).
REACT_199093. Cross-presentation of particulate exogenous antigens (phagosomes).

Miscellaneous databases

NextBioi 292917.
PROi O70145.
SOURCEi Search...

Gene expression databases

Bgeei O70145.
CleanExi MM_NCF2.
Genevestigatori O70145.

Family and domain databases

Gene3Di 1.25.40.10. 1 hit.
InterProi IPR000270. OPR_PB1.
IPR001452. SH3_domain.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view ]
Pfami PF00564. PB1. 1 hit.
PF00018. SH3_1. 2 hits.
PF00515. TPR_1. 1 hit.
[Graphical view ]
PRINTSi PR00452. SH3DOMAIN.
SMARTi SM00666. PB1. 1 hit.
SM00326. SH3. 2 hits.
SM00028. TPR. 3 hits.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 2 hits.
PROSITEi PS50002. SH3. 2 hits.
PS50005. TPR. 3 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Functional modules and expression of mouse p40(phox) and p67(phox), SH3-domain-containing proteins involved in the phagocyte NADPH oxidase complex."
    Mizuki K., Kadomatsu K., Hata K., Ito T., Fan Q.-W., Kage Y., Fukumaki Y., Sakaki Y., Takeshige K., Sumimoto H.
    Eur. J. Biochem. 251:573-582(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Myelomonocyte.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6JImported and NODImported.
    Tissue: BoneImported, Dendritic cell, Macrophage and Urinary bladderImported.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/NImported.
    Tissue: Mammary glandImported.
  4. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNCF2_MOUSE
AccessioniPrimary (citable) accession number: O70145
Secondary accession number(s): Q3TC92, Q3U5S4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: August 1, 1998
Last modified: October 29, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3