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O70145

- NCF2_MOUSE

UniProt

O70145 - NCF2_MOUSE

Protein

Neutrophil cytosol factor 2

Gene

Ncf2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).By similarity

    GO - Molecular functioni

    1. protein binding Source: MGI
    2. Rac GTPase binding Source: MGI

    GO - Biological processi

    1. aging Source: Ensembl
    2. cellular response to hormone stimulus Source: Ensembl
    3. NADP catabolic process Source: MGI
    4. oxidation-reduction process Source: GOC
    5. positive regulation of blood pressure Source: Ensembl
    6. positive regulation of neuron apoptotic process Source: Ensembl
    7. response to activity Source: Ensembl
    8. response to drug Source: Ensembl
    9. response to glucose Source: Ensembl
    10. response to hyperoxia Source: Ensembl
    11. response to laminar fluid shear stress Source: Ensembl
    12. response to lipopolysaccharide Source: Ensembl
    13. response to progesterone Source: Ensembl
    14. superoxide anion generation Source: MGI
    15. superoxide metabolic process Source: MGI

    Enzyme and pathway databases

    ReactomeiREACT_198345. Phagosomal maturation (early endosomal stage).
    REACT_199093. Cross-presentation of particulate exogenous antigens (phagosomes).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neutrophil cytosol factor 2
    Short name:
    NCF-2
    Alternative name(s):
    67 kDa neutrophil oxidase factor
    NADPH oxidase activator 2
    Neutrophil NADPH oxidase factor 2
    p67-phox
    Gene namesi
    Name:Ncf2Imported
    Synonyms:Noxa2, P67phox
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:97284. Ncf2.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. acrosomal vesicle Source: MGI
    2. cytoplasm Source: MGI
    3. cytosol Source: MGI
    4. membrane Source: Ensembl
    5. nucleolus Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 525525Neutrophil cytosol factor 2PRO_0000312227Add
    BLAST

    Proteomic databases

    MaxQBiO70145.
    PaxDbiO70145.
    PRIDEiO70145.

    PTM databases

    PhosphoSiteiO70145.

    Expressioni

    Gene expression databases

    BgeeiO70145.
    CleanExiMM_NCF2.
    GenevestigatoriO70145.

    Interactioni

    Subunit structurei

    Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF4. Interacts (via the C-terminal SH3 domain) with NCF1 (via C-terminus). Interacts with SYTL1 and RAC1. May interact with NOXO1. Interacts with S100A8 and calprotectin (S100A8/9) By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO70145.
    SMRiO70145. Positions 2-193, 242-297, 351-426, 454-514.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati37 – 7034TPR 1Sequence AnalysisAdd
    BLAST
    Repeati71 – 10434TPR 2Sequence AnalysisAdd
    BLAST
    Repeati121 – 15434TPR 3Sequence AnalysisAdd
    BLAST
    Domaini240 – 29960SH3 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini350 – 42879OPRSequence AnalysisAdd
    BLAST
    Domaini456 – 51560SH3 2PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The OPR/PB1 domain mediates the association with NCF4/p40-PHOX.By similarity

    Sequence similaritiesi

    Belongs to the NCF2/NOXA1 family.Curated
    Contains 1 OPR domain.Sequence Analysis
    Contains 2 SH3 domains.PROSITE-ProRule annotation
    Contains 3 TPR repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, SH3 domain, TPR repeat

    Phylogenomic databases

    eggNOGiNOG248396.
    GeneTreeiENSGT00530000063843.
    HOGENOMiHOG000237312.
    HOVERGENiHBG001521.
    InParanoidiO70145.
    KOiK08010.
    OMAiYSQVRDM.
    OrthoDBiEOG7G1V62.
    PhylomeDBiO70145.
    TreeFamiTF329087.

    Family and domain databases

    Gene3Di1.25.40.10. 1 hit.
    InterProiIPR000270. OPR_PB1.
    IPR001452. SH3_domain.
    IPR013026. TPR-contain_dom.
    IPR011990. TPR-like_helical.
    IPR001440. TPR_1.
    IPR019734. TPR_repeat.
    [Graphical view]
    PfamiPF00564. PB1. 1 hit.
    PF00018. SH3_1. 2 hits.
    PF00515. TPR_1. 1 hit.
    [Graphical view]
    PRINTSiPR00452. SH3DOMAIN.
    SMARTiSM00666. PB1. 1 hit.
    SM00326. SH3. 2 hits.
    SM00028. TPR. 3 hits.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 2 hits.
    PROSITEiPS50002. SH3. 2 hits.
    PS50005. TPR. 3 hits.
    PS50293. TPR_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O70145-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLAEAIRLW NEGVLAADKK DWKGALEAFS EVQDPHSRIC FNIGCVNTIL    50
    ENLQAAEQAF TKSINRDKHS AVAYFQRGML YYRMEKYDLA IKDLKEALTQ 100
    LRGNQLIDYK ILGLQFKLFA CEVLYNIALM HAKKEEWKKA EEQLALATNM 150
    KSEPRHSKID KAMESIWKQK LFEPVVIPVG RLFRPNERQV AQLAKKDYLG 200
    KATVVASVVH QDNFSGFAPL QPQSAEPPPR PKTPEIFRAL EGEAHRVLFG 250
    FVPETPEELQ VMPGNIVFVL KKGSDNWATV MFNGQKGLVP CNYLEPVELR 300
    IHPQSQPQED TSPESDIPPP PNSSPPGRLQ LSPGHKQKEP KELKLSVPMP 350
    YMLKVHYKYT VVMETRLGLP YSQLRNMVSK KLALSPEHTK LSYRRRDSHE 400
    LLLLSEESMK DAWGQVKNYC LTLWCEHTVG DQGLIDEPIQ RENSDASKQT 450
    TEPQPKEGTQ VVAIFSYEAA QPEDLEFVEG DVILVLSHVN EEWLEGECKG 500
    KVGIFPKAFV EGCAAKNLEG IPREV 525
    Length:525
    Mass (Da):59,485
    Last modified:August 1, 1998 - v1
    Checksum:i663DB6B52D790F76
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti31 – 311E → A in BAE42353. (PubMed:16141072)Curated
    Sequence conflicti31 – 311E → A in BAE42218. (PubMed:16141072)Curated
    Sequence conflicti31 – 311E → A in BAE42065. (PubMed:16141072)Curated
    Sequence conflicti31 – 311E → A in BAE33191. (PubMed:16141072)Curated
    Sequence conflicti324 – 3241S → N in BAE32002. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB002664 mRNA. Translation: BAA25650.1.
    AK036379 mRNA. Translation: BAC29404.1.
    AK137152 mRNA. Translation: BAE23254.1.
    AK153447 mRNA. Translation: BAE32002.1.
    AK155324 mRNA. Translation: BAE33191.1.
    AK170839 mRNA. Translation: BAE42065.1.
    AK171059 mRNA. Translation: BAE42218.1.
    AK171265 mRNA. Translation: BAE42353.1.
    BC003730 mRNA. Translation: AAH03730.1.
    CCDSiCCDS15367.1.
    RefSeqiNP_035007.1. NM_010877.4.
    XP_006529298.1. XM_006529235.1.
    XP_006529299.1. XM_006529236.1.
    UniGeneiMm.270307.

    Genome annotation databases

    EnsembliENSMUST00000027754; ENSMUSP00000027754; ENSMUSG00000026480.
    GeneIDi17970.
    KEGGimmu:17970.
    UCSCiuc007czm.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB002664 mRNA. Translation: BAA25650.1 .
    AK036379 mRNA. Translation: BAC29404.1 .
    AK137152 mRNA. Translation: BAE23254.1 .
    AK153447 mRNA. Translation: BAE32002.1 .
    AK155324 mRNA. Translation: BAE33191.1 .
    AK170839 mRNA. Translation: BAE42065.1 .
    AK171059 mRNA. Translation: BAE42218.1 .
    AK171265 mRNA. Translation: BAE42353.1 .
    BC003730 mRNA. Translation: AAH03730.1 .
    CCDSi CCDS15367.1.
    RefSeqi NP_035007.1. NM_010877.4.
    XP_006529298.1. XM_006529235.1.
    XP_006529299.1. XM_006529236.1.
    UniGenei Mm.270307.

    3D structure databases

    ProteinModelPortali O70145.
    SMRi O70145. Positions 2-193, 242-297, 351-426, 454-514.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei O70145.

    Proteomic databases

    MaxQBi O70145.
    PaxDbi O70145.
    PRIDEi O70145.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000027754 ; ENSMUSP00000027754 ; ENSMUSG00000026480 .
    GeneIDi 17970.
    KEGGi mmu:17970.
    UCSCi uc007czm.1. mouse.

    Organism-specific databases

    CTDi 4688.
    MGIi MGI:97284. Ncf2.

    Phylogenomic databases

    eggNOGi NOG248396.
    GeneTreei ENSGT00530000063843.
    HOGENOMi HOG000237312.
    HOVERGENi HBG001521.
    InParanoidi O70145.
    KOi K08010.
    OMAi YSQVRDM.
    OrthoDBi EOG7G1V62.
    PhylomeDBi O70145.
    TreeFami TF329087.

    Enzyme and pathway databases

    Reactomei REACT_198345. Phagosomal maturation (early endosomal stage).
    REACT_199093. Cross-presentation of particulate exogenous antigens (phagosomes).

    Miscellaneous databases

    NextBioi 292917.
    PROi O70145.
    SOURCEi Search...

    Gene expression databases

    Bgeei O70145.
    CleanExi MM_NCF2.
    Genevestigatori O70145.

    Family and domain databases

    Gene3Di 1.25.40.10. 1 hit.
    InterProi IPR000270. OPR_PB1.
    IPR001452. SH3_domain.
    IPR013026. TPR-contain_dom.
    IPR011990. TPR-like_helical.
    IPR001440. TPR_1.
    IPR019734. TPR_repeat.
    [Graphical view ]
    Pfami PF00564. PB1. 1 hit.
    PF00018. SH3_1. 2 hits.
    PF00515. TPR_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00452. SH3DOMAIN.
    SMARTi SM00666. PB1. 1 hit.
    SM00326. SH3. 2 hits.
    SM00028. TPR. 3 hits.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 2 hits.
    PROSITEi PS50002. SH3. 2 hits.
    PS50005. TPR. 3 hits.
    PS50293. TPR_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Functional modules and expression of mouse p40(phox) and p67(phox), SH3-domain-containing proteins involved in the phagocyte NADPH oxidase complex."
      Mizuki K., Kadomatsu K., Hata K., Ito T., Fan Q.-W., Kage Y., Fukumaki Y., Sakaki Y., Takeshige K., Sumimoto H.
      Eur. J. Biochem. 251:573-582(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Myelomonocyte.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6JImported and NODImported.
      Tissue: BoneImported, Dendritic cell, Macrophage and Urinary bladderImported.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/NImported.
      Tissue: Mammary glandImported.
    4. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNCF2_MOUSE
    AccessioniPrimary (citable) accession number: O70145
    Secondary accession number(s): Q3TC92, Q3U5S4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 4, 2007
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3