O70133 (DHX9_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent RNA helicase A
Short name=RHA
EC=3.6.4.13

Alternative name(s):
DEAH box protein 9
Short name=mHEL-5
Nuclear DNA helicase II
Short name=NDH II

Gene names

Name:	Dhx9
Synonyms:	Ddx9

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1380 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the CRD-mediated complex that promotes MYC mRNA stability. Unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alterations of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2 By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Component of the coding region determinant (CRD)-mediated complex, composed of DHX9, HNRNPU, IGF2BP1, SYNCRIP and YBX1. Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with IGF2BP1. Binds MBD2, HRMT1L2/PRMT1, and RELA. May act to directly link BRCA1, CREBBP or SMN1 and the RNA polymerase II complex. Can also interact with XRCC5 and with TOP2A in an RNA dependent manner; these interactions may be indirect. Interaction with TOP2A is promoted by UBC9. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on 'Ser-139' By similarity. Interacts with LARP6 By similarity. Interacts with ZIC2. Ref.9
Subcellular location	Nucleus › nucleolus. Cytoplasm By similarity. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Can shuttle between nucleus and cytoplasm By similarity. Ref.6
Domain	The MTAD domain mediates interaction with the RNA polymerase II holoenzyme By similarity.
Post-translational modification	Methylated By similarity. May be phosphorylated by PRKDC/XRCC7 By similarity.
Sequence similarities	Belongs to the DEAD box helicase family. DEAH subfamily. Contains 2 DRBM (double-stranded RNA-binding) domains. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Sequence caution	The sequence AAH89159.1 differs from that shown. Reason: Frameshift at positions 447 and 448. The sequence AAR87796.1 differs from that shown. Reason: Intron retention.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	ATP-binding DNA-binding Nucleotide-binding RNA-binding
Molecular function	Activator Helicase Hydrolase
PTM	Acetylation Methylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to heat Inferred from direct assay PubMed 18755693. Source: MGI circadian rhythm Inferred from mutant phenotype PubMed 22767893. Source: MGI
Cellular_component	CRD-mediated mRNA stability complex Inferred from sequence or structural similarity. Source: UniProtKB nucleolus Inferred from direct assay PubMed 14729462. Source: MGI ribonucleoprotein complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: UniProtKB-KW double-stranded RNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O70133-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O70133-2)

The sequence of this isoform differs from the canonical sequence as follows:
     123-123: E → EA

Isoform 3 (identifier: O70133-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-216: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1380

1380

ATP-dependent RNA helicase A

PRO_0000055158

Regions

Domain

3 – 71

69

DRBM 1

Domain

182 – 254

73

DRBM 2

Domain

400 – 566

167

Helicase ATP-binding

Domain

638 – 811

174

Helicase C-terminal

Nucleotide binding

413 – 420

8

ATP By similarity

Region

1 – 253

253

Interaction with CREBBP By similarity

Region

233 – 328

96

Interaction with BRCA1 By similarity

Region

333 – 382

50

MTAD By similarity

Motif

513 – 516

4

DEAH box

Motif

588 – 597

10

Nuclear localization signal Potential

Compositional bias

1171 – 1380

210

Arg/Gly/Ser/Tyr-rich

Amino acid modifications

Modified residue

193

1

N6-acetyllysine By similarity

Modified residue

201

1

N6-acetyllysine By similarity

Modified residue

323

1

Phosphoserine Ref.7

Modified residue

1026

1

N6-acetyllysine By similarity

Natural variations

Alternative sequence

1 – 216

216

Missing in isoform 3.

VSP_014778

Alternative sequence

123

1

E → EA in isoform 2.

VSP_014779

Experimental info

Sequence conflict

46

1

A → R in AAC05725. Ref.1

Sequence conflict

136

1

S → A in AAC05725. Ref.1

Sequence conflict

136

1

S → A in AAC05301. Ref.1

Sequence conflict

187

1

L → V Ref.2

Sequence conflict

189

1

Q → H in AAC05725. Ref.1

Sequence conflict

211

1

R → G in BAB28848. Ref.4

Sequence conflict

235

1

S → C in AAC05725. Ref.1

Sequence conflict

257

1

V → C in AAC05725. Ref.1

Sequence conflict

281

1

S → P in AAC05725. Ref.1

Sequence conflict

674

1

N → M in AAB72087. Ref.5

Sequence conflict

748

1

T → I in AAB72087. Ref.5

Sequence conflict

831

1

I → V in AAB72087. Ref.5

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

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.

.

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.

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.

.

.

.

.

1380

Helix Strand Turn

Details...

Helix

5 – 14

10

Beta strand

20 – 27

8

Beta strand

29 – 39

11

Beta strand

47 – 53

7

Helix

54 – 72

19

Turn

77 – 79

3

Helix

172 – 175

4

Helix

180 – 193

14

Beta strand

201 – 206

6

Beta strand

212 – 221

10

Turn

222 – 225

4

Beta strand

226 – 231

6

Helix

237 – 255

19

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 19, 2005. Version 2. Checksum: 005D641CD9A4F0C9	FASTA	1,380	149,475
	10 20 30 40 50 60 MGDIKNFLYA WCGKRKMTPA YEIRAVGNKN RQKFMCEVRV EGFNYAGMGN STNKKDAQSN 70 80 90 100 110 120 AARDFVNYLV RINEVKSEEV PAVGIVPPPP ILSDTSDSTA SAAEGLPAPM GGPLPPHLAL 130 140 150 160 170 180 KAEENNSGVE SSGYGSPGPT WDRGANLKDY YSRKEEQEVQ ATLESEEVDL NAGLHGNWTL 190 200 210 220 230 240 ENAKARLNQY FQKEKIQGEY KYTQVGPDHN RSFIAEMTIY IKQLGRRIFA REHGSNKKLA 250 260 270 280 290 300 AQSCALSLVR QLYHLGVIEA YSGLTKKKEG ERVEPYKVFL SPDLELQLQN VVQELDLEIV 310 320 330 340 350 360 PPPVDPSMPV ILNIGKLAHF EPSQRQNAVG VVPWSPPQSN WNPWTSSNID EGPLAYASTE 370 380 390 400 410 420 QISMDLKNEL TYQMEQDHNL QSVLQERELL PVKKFEAEIL EAISSNSVVI IRGATGCGKT 430 440 450 460 470 480 TQVPQYILDD FIQNDRAAEC NIVVTQPRRI SAVAVAERVA YERGEEPGKS CGYSVRFESI 490 500 510 520 530 540 LPRPHASIMF CTVGVLLRKL EAGIRGISHV IVDEIHERDI NTDFLLVVLR DVVLAYPEVR 550 560 570 580 590 600 IVLMSATIDT TMFCEYFFNC PIIEVYGRTF PVQEYFLEDC IQMTQFIPPP KDKKKKDKED 610 620 630 640 650 660 DGGEDDDANC NLICGDEYGP ETKLSMSQLN EKETPFELIE ALLKYIETLN VPGAVLVFLP 670 680 690 700 710 720 GWNLIYTMQK HLENNSHFGS HRYQILPLHS QIPREEQRKV FDPVPDGVTK VILSTNIAET 730 740 750 760 770 780 SITINDVVYV IDSCKQKVKL FTAHNNMTNY ATVWASKTNL EQRKGRAGRV RPGFCFHLCS 790 800 810 820 830 840 RARFDRLETH MTPEMFRTPL HEIALSIKLL RLGGIGQFLA KAIEPPPLDA IIEAEHTLRE 850 860 870 880 890 900 LDALDANDEL TPLGRILAKL PIEPRFGKMM IMGCIFYVGD AVCTISAATC FPEPFISEGK 910 920 930 940 950 960 RLGYIHRNFA GNRFSDHVAL LSVFQAWDDA RMSGEEAEIR FCEQKRLNMA TLRMTWEAKV 970 980 990 1000 1010 1020 QLKEILINSG FPEDCLLTQV FTNTGPDNNL DVVISLLAFG VYPNVCYHKE KRKILTTEGR 1030 1040 1050 1060 1070 1080 NALIHKSSVN CPFSSQDMKY PSPFFVFGEK IRTRAISAKG MTLVTPLQLL LFASKKVQSD 1090 1100 1110 1120 1130 1140 GQIVFIDDWI RLQISHEAAA CITIRAAMEA LVVEVSKQPN IISQLDPVNE HMLNTIRQIS 1150 1160 1170 1180 1190 1200 RPSAAGINLM IGSVRYGDGP RPPKMARYDN GSGYRRGYGG GGYGGGGYGG GYGSGGFGGG 1210 1220 1230 1240 1250 1260 FGSGGGFGGG FNSGGGGFGS GGGGFGSGGG GFGGGGGGFS GGGGGGFGGG RGGGGGGFGG 1270 1280 1290 1300 1310 1320 SGGFGNGGGG YGVGGGGYGG GGGGGYGGGS GGYGGGGYGG GEGYSISPNS YRGNYGGGGG 1330 1340 1350 1360 1370 1380 GYRGGSQGGY RNNFGGDYRG SSGDYRGSGG GYRGSGGFQR RGYGGGYFGQ GRGGGGGGGY
	Isoform 2 [UniParc]. Checksum: 0AB5502F91DEFB73	FASTA	1,381	149,546
	10 20 30 40 50 60 MGDIKNFLYA WCGKRKMTPA YEIRAVGNKN RQKFMCEVRV EGFNYAGMGN STNKKDAQSN 70 80 90 100 110 120 AARDFVNYLV RINEVKSEEV PAVGIVPPPP ILSDTSDSTA SAAEGLPAPM GGPLPPHLAL 130 140 150 160 170 180 KAEAENNSGV ESSGYGSPGP TWDRGANLKD YYSRKEEQEV QATLESEEVD LNAGLHGNWT 190 200 210 220 230 240 LENAKARLNQ YFQKEKIQGE YKYTQVGPDH NRSFIAEMTI YIKQLGRRIF AREHGSNKKL 250 260 270 280 290 300 AAQSCALSLV RQLYHLGVIE AYSGLTKKKE GERVEPYKVF LSPDLELQLQ NVVQELDLEI 310 320 330 340 350 360 VPPPVDPSMP VILNIGKLAH FEPSQRQNAV GVVPWSPPQS NWNPWTSSNI DEGPLAYAST 370 380 390 400 410 420 EQISMDLKNE LTYQMEQDHN LQSVLQEREL LPVKKFEAEI LEAISSNSVV IIRGATGCGK 430 440 450 460 470 480 TTQVPQYILD DFIQNDRAAE CNIVVTQPRR ISAVAVAERV AYERGEEPGK SCGYSVRFES 490 500 510 520 530 540 ILPRPHASIM FCTVGVLLRK LEAGIRGISH VIVDEIHERD INTDFLLVVL RDVVLAYPEV 550 560 570 580 590 600 RIVLMSATID TTMFCEYFFN CPIIEVYGRT FPVQEYFLED CIQMTQFIPP PKDKKKKDKE 610 620 630 640 650 660 DDGGEDDDAN CNLICGDEYG PETKLSMSQL NEKETPFELI EALLKYIETL NVPGAVLVFL 670 680 690 700 710 720 PGWNLIYTMQ KHLENNSHFG SHRYQILPLH SQIPREEQRK VFDPVPDGVT KVILSTNIAE 730 740 750 760 770 780 TSITINDVVY VIDSCKQKVK LFTAHNNMTN YATVWASKTN LEQRKGRAGR VRPGFCFHLC 790 800 810 820 830 840 SRARFDRLET HMTPEMFRTP LHEIALSIKL LRLGGIGQFL AKAIEPPPLD AIIEAEHTLR 850 860 870 880 890 900 ELDALDANDE LTPLGRILAK LPIEPRFGKM MIMGCIFYVG DAVCTISAAT CFPEPFISEG 910 920 930 940 950 960 KRLGYIHRNF AGNRFSDHVA LLSVFQAWDD ARMSGEEAEI RFCEQKRLNM ATLRMTWEAK 970 980 990 1000 1010 1020 VQLKEILINS GFPEDCLLTQ VFTNTGPDNN LDVVISLLAF GVYPNVCYHK EKRKILTTEG 1030 1040 1050 1060 1070 1080 RNALIHKSSV NCPFSSQDMK YPSPFFVFGE KIRTRAISAK GMTLVTPLQL LLFASKKVQS 1090 1100 1110 1120 1130 1140 DGQIVFIDDW IRLQISHEAA ACITIRAAME ALVVEVSKQP NIISQLDPVN EHMLNTIRQI 1150 1160 1170 1180 1190 1200 SRPSAAGINL MIGSVRYGDG PRPPKMARYD NGSGYRRGYG GGGYGGGGYG GGYGSGGFGG 1210 1220 1230 1240 1250 1260 GFGSGGGFGG GFNSGGGGFG SGGGGFGSGG GGFGGGGGGF SGGGGGGFGG GRGGGGGGFG 1270 1280 1290 1300 1310 1320 GSGGFGNGGG GYGVGGGGYG GGGGGGYGGG SGGYGGGGYG GGEGYSISPN SYRGNYGGGG 1330 1340 1350 1360 1370 1380 GGYRGGSQGG YRNNFGGDYR GSSGDYRGSG GGYRGSGGFQ RRGYGGGYFG QGRGGGGGGG Y
	Isoform 3 [UniParc]. Checksum: DA64E679A88D87C2	FASTA	1,164	125,615
	10 20 30 40 50 60 MTIYIKQLGR RIFAREHGSN KKLAAQSCAL SLVRQLYHLG VIEAYSGLTK KKEGERVEPY 70 80 90 100 110 120 KVFLSPDLEL QLQNVVQELD LEIVPPPVDP SMPVILNIGK LAHFEPSQRQ NAVGVVPWSP 130 140 150 160 170 180 PQSNWNPWTS SNIDEGPLAY ASTEQISMDL KNELTYQMEQ DHNLQSVLQE RELLPVKKFE 190 200 210 220 230 240 AEILEAISSN SVVIIRGATG CGKTTQVPQY ILDDFIQNDR AAECNIVVTQ PRRISAVAVA 250 260 270 280 290 300 ERVAYERGEE PGKSCGYSVR FESILPRPHA SIMFCTVGVL LRKLEAGIRG ISHVIVDEIH 310 320 330 340 350 360 ERDINTDFLL VVLRDVVLAY PEVRIVLMSA TIDTTMFCEY FFNCPIIEVY GRTFPVQEYF 370 380 390 400 410 420 LEDCIQMTQF IPPPKDKKKK DKEDDGGEDD DANCNLICGD EYGPETKLSM SQLNEKETPF 430 440 450 460 470 480 ELIEALLKYI ETLNVPGAVL VFLPGWNLIY TMQKHLENNS HFGSHRYQIL PLHSQIPREE 490 500 510 520 530 540 QRKVFDPVPD GVTKVILSTN IAETSITIND VVYVIDSCKQ KVKLFTAHNN MTNYATVWAS 550 560 570 580 590 600 KTNLEQRKGR AGRVRPGFCF HLCSRARFDR LETHMTPEMF RTPLHEIALS IKLLRLGGIG 610 620 630 640 650 660 QFLAKAIEPP PLDAIIEAEH TLRELDALDA NDELTPLGRI LAKLPIEPRF GKMMIMGCIF 670 680 690 700 710 720 YVGDAVCTIS AATCFPEPFI SEGKRLGYIH RNFAGNRFSD HVALLSVFQA WDDARMSGEE 730 740 750 760 770 780 AEIRFCEQKR LNMATLRMTW EAKVQLKEIL INSGFPEDCL LTQVFTNTGP DNNLDVVISL 790 800 810 820 830 840 LAFGVYPNVC YHKEKRKILT TEGRNALIHK SSVNCPFSSQ DMKYPSPFFV FGEKIRTRAI 850 860 870 880 890 900 SAKGMTLVTP LQLLLFASKK VQSDGQIVFI DDWIRLQISH EAAACITIRA AMEALVVEVS 910 920 930 940 950 960 KQPNIISQLD PVNEHMLNTI RQISRPSAAG INLMIGSVRY GDGPRPPKMA RYDNGSGYRR 970 980 990 1000 1010 1020 GYGGGGYGGG GYGGGYGSGG FGGGFGSGGG FGGGFNSGGG GFGSGGGGFG SGGGGFGGGG 1030 1040 1050 1060 1070 1080 GGFSGGGGGG FGGGRGGGGG GFGGSGGFGN GGGGYGVGGG GYGGGGGGGY GGGSGGYGGG 1090 1100 1110 1120 1130 1140 GYGGGEGYSI SPNSYRGNYG GGGGGYRGGS QGGYRNNFGG DYRGSSGDYR GSGGGYRGSG 1150 1160 GFQRRGYGGG YFGQGRGGGG GGGY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular analysis of the cDNA and genomic DNA encoding mouse RNA helicase A." Lee C.-G., Eki T., Okumura K., da Costa Soares V., Hurwitz J. Genomics 47:365-371(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-161. Strain: 129/Sv.
[2]	"Transcriptome analysis of mouse stem cells and early embryos." Sharov A.A., Piao Y., Matoba R., Dudekula D.B., Qian Y., VanBuren V., Falco G., Martin P.R., Stagg C.A., Bassey U.C., Wang Y., Carter M.G., Hamatani T., Aiba K., Akutsu H., Sharova L., Tanaka T.S., Kimber W.L. , Yoshikawa T., Jaradat S.A., Pantano S., Nagaraja R., Boheler K.R., Taub D., Hodes R.J., Longo D.L., Schlessinger D., Keller J., Klotz E., Kelsoe G., Umezawa A., Vescovi A.L., Rossant J., Kunath T., Hogan B.L.M., Curci A., D'Urso M., Kelso J., Hide W., Ko M.S.H. PLoS Biol. 1:410-419(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-756 (ISOFORM 3). Strain: C57BL/6NCr.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-756 (ISOFORM 1). Strain: C57BL/6J. Tissue: Embryonic germ cell.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-264 (ISOFORM 2). Strain: C57BL/6J. Tissue: Embryo.
[5]	"mHEL-5." Kisielow P., Miazek A. Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 386-919 (ISOFORMS 1/2/3). Strain: C57BL/6.
[6]	"Nucleolar localization of murine nuclear DNA helicase II (RNA helicase A)." Zhang S., Herrmann C., Grosse F. J. Cell Sci. 112:2693-2703(1999) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[7]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-323, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[8]	"Solution structure of double-stranded RNA-binding motifs from hypothetical protein BAB28848." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 4-262.
[9]	"Functional role of Zic2 phosphorylation in transcriptional regulation." Ishiguro A., Aruga J. FEBS Lett. 582:154-158(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ZIC2.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U91922 mRNA. Translation: AAC05725.1.
AF023530 Genomic DNA. Translation: AAC05301.1.
AY512925 mRNA. Translation: AAR87796.1. Sequence problems.
BC089159 mRNA. Translation: AAH89159.1. Sequence problems.
AK013423 mRNA. Translation: BAB28848.1.
U92080 mRNA. Translation: AAB72087.1.

IPI

IPI00339468.
IPI00607914.
IPI00875791.

RefSeq

NP_031868.2. NM_007842.2.

UniGene

Mm.20000.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1UIL	NMR	-	A	163-262	[»]
1WHQ	NMR	-	A	4-89	[»]
2RS6	NMR	-	A	4-89	[»]
2RS7	NMR	-	A	163-262	[»]

ProteinModelPortal

O70133.

SMR

O70133. Positions 4-89, 168-262, 331-1073.

ModBase

Search...

Protein-protein interaction databases

IntAct

O70133. 4 interactions.

PTM databases

PhosphoSite

O70133.

Proteomic databases

PaxDb

O70133.

PRIDE

O70133.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

13211.

KEGG

mmu:13211.

UCSC

uc007czy.1. mouse.

Organism-specific databases

CTD

1660.

MGI

MGI:108177. Dhx9.

Phylogenomic databases

eggNOG

COG1643.

HOGENOM

HOG000247063.

HOVERGEN

HBG039429.

InParanoid

O70133.

KO

K13184.

OrthoDB

EOG4HHP1J.

Gene expression databases

CleanEx

MM_DHX9.

Genevestigator

O70133.

GermOnline

ENSMUSG00000042699. Mus musculus.

Family and domain databases

Gene3D

3.30.160.20. 2 hits.

InterPro

IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR001159. Ds-RNA-bd.
IPR014720. dsRNA-bd-like_dom.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
[Graphical view]

Pfam

PF00270. DEAD. 1 hit.
PF00035. dsrm. 2 hits.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]

SMART

SM00487. DEXDc. 1 hit.
SM00358. DSRM. 2 hits.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]

PROSITE

PS00690. DEAH_ATP_HELICASE. 1 hit.
PS50137. DS_RBD. 2 hits.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

ProtoNet

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Other

ChiTaRS

DHX9. mouse.

EvolutionaryTrace

O70133.

NextBio

283382.

SOURCE

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Entry information

Entry name

DHX9_MOUSE

Accession

Primary (citable) accession number: O70133
Secondary accession number(s): O35931 , O54703, Q5FWY1, Q6R5F7, Q9CSA2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 19, 2005
Last modified:	May 1, 2013
This is version 118 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program