Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O70127

- ABCBB_RAT

UniProt

O70127 - ABCBB_RAT

Protein

Bile salt export pump

Gene

Abcb11

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi455 – 4628ATP 1PROSITE-ProRule annotation
    Nucleotide bindingi1113 – 11208ATP 2PROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATPase activity, coupled to transmembrane movement of substances Source: RefGenome
    2. ATP binding Source: UniProtKB-KW
    3. canalicular bile acid transmembrane transporter activity Source: RGD
    4. drug transmembrane transporter activity Source: RGD
    5. protein binding Source: IntAct

    GO - Biological processi

    1. canalicular bile acid transport Source: RGD
    2. drug export Source: RGD
    3. drug transmembrane transport Source: GOC
    4. response to drug Source: RGD
    5. response to estrogen Source: RGD
    6. response to oxidative stress Source: RGD

    Keywords - Biological processi

    Transport

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bile salt export pump
    Alternative name(s):
    ATP-binding cassette sub-family B member 11
    Sister of P-glycoprotein
    Gene namesi
    Name:Abcb11
    Synonyms:Bsep, Spgp
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi619930. Abcb11.

    Subcellular locationi

    GO - Cellular componenti

    1. apical plasma membrane Source: RGD
    2. Golgi apparatus Source: RGD
    3. Golgi membrane Source: RGD
    4. integral component of membrane Source: RefGenome
    5. plasma membrane Source: RGD

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13211321Bile salt export pumpPRO_0000093299Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi109 – 1091N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi116 – 1161N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi122 – 1221N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi125 – 1251N-linked (GlcNAc...)Sequence Analysis
    Modified residuei692 – 6921PhosphoserineBy similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PRIDEiO70127.

    PTM databases

    PhosphoSiteiO70127.

    Expressioni

    Tissue specificityi

    Expressed predominantly, if not exclusively in the liver, where it was further localized to the canalicular microvilli and to subcanalicular vesicles of the hepatocytes by in situ.

    Gene expression databases

    GenevestigatoriO70127.

    Interactioni

    Subunit structurei

    Interacts with HAX1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Hax1Q7TSE95EBI-930036,EBI-930005

    Protein-protein interaction databases

    BioGridi249756. 1 interaction.
    IntActiO70127. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliO70127.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 6262CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini84 – 14764ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini169 – 21547CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini237 – 2404ExtracellularSequence Analysis
    Topological domaini262 – 31958CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini341 – 35313ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini375 – 755381CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini777 – 79418ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini816 – 86954CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini912 – 97968CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini1001 – 101111ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1033 – 1321289CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei63 – 8321HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei148 – 16821HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei216 – 23621HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei241 – 26121HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei320 – 34021HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei354 – 37421HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei756 – 77621HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei795 – 81521HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei870 – 89021HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei891 – 91121HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei980 – 100021HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei1012 – 103221HelicalPROSITE-ProRule annotationAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini62 – 385324ABC transmembrane type-1 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini420 – 656237ABC transporter 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini755 – 1043289ABC transmembrane type-1 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini1078 – 1316239ABC transporter 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni651 – 67424Interaction with HAX1Add
    BLAST

    Domaini

    Multifunctional polypeptide with two homologous halves, each containing a hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.

    Sequence similaritiesi

    Contains 2 ABC transmembrane type-1 domains.PROSITE-ProRule annotation
    Contains 2 ABC transporter domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOVERGENiHBG080809.
    KOiK05664.
    PhylomeDBiO70127.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR011527. ABC1_TM_dom.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR001140. ABC_transptr_TM_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00664. ABC_membrane. 2 hits.
    PF00005. ABC_tran. 2 hits.
    [Graphical view]
    SMARTiSM00382. AAA. 2 hits.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    SSF90123. SSF90123. 3 hits.
    PROSITEiPS50929. ABC_TM1F. 2 hits.
    PS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O70127-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDSVILRSV KKFGEENHAF ESDGSHNNDK KSRLQDKMKE GDIRVGFFEL     50
    FRFSSSKDIW LMLMGGVCAL LHGMAQPGIL IIFGIMTDIF IKYDIERQEL 100
    EIPGKACVNN TIVWINSSFH QNMTNGTVCG LVDIESEMIK FSGIYAGVGM 150
    TVLILGYFQI RLWVITGARQ IRRMRKIYFR RIMRMEIGWF DCTSVGELNS 200
    RFADDIEKIN DAIADQLAHF LQRMSTAMCG LLLGFYRGWK LTLVILAVSP 250
    LIGIGAAVIG LSIAKFTELE LKAYAKAGSI ADEVLSSIRT VAAFGGENKE 300
    VERYEKNLVF AQRWGIWKGM VMGFFTGYMW CLIFFCYALA FWYGSTLVLD 350
    EEEYTPGTLV QIFLCVILAA MNIGHASSCL EIFSTGCSAA TNIFQTIDRQ 400
    PVIDCMSGDG YKLDRIKGEI EFHNVTFHYP SRPDVKILDN LSMVIKPGET 450
    TALVGSSGAG KSTALQLIQR FYDPCEGMVT LDGHDIRSLN IRWLRDQIGI 500
    VEQEPVLFST TIAENIRFGR EDATMEDIVQ AAKDANAYNF IMALPQQFDT 550
    LVGEGGGQMS GGQKQRVAIA RALIRNPKIL LLDMATSALD NESEARVQEA 600
    LNKIQHGHTI ISVAHRLSTV RAADVIIGFE HGVAVERGTH EELLERKGVY 650
    FMLVTLQSQG DNAHKETSIM GKDATEGGTL ERTFSRGSYR DSLRASIRQR 700
    SKSQLSLLTH DPPLAVADHK SSYKDSKDND VLVEEVEPAP VRRILKYNIP 750
    EWHYILVGSL SAAINGAVTP IYSLLFSQLL GTFSLLDKEQ QRSEIHSMCL 800
    FFVILGCVSI FTQFLQGYTF AKSGELLTKR LRKFGFKAML GQDIGWFDDL 850
    RNNPGVLTTR LATDASQVQG ATGSQVGMMV NSFTNIIAAL LIAFFFSWKL 900
    SLIITIFFPF LALSGAVQTK MLTGFASQDK QALEKAGQIT SEALSNIRTV 950
    AGIGVEGRFI KAFEVELQTS YKTAVRKANI YGLCFAFSQG IAFLANSAAY 1000
    RYGGYLIAYE GLGFSHVFRV VSSVALSATA VGRTFSYTPS YAKAKISAAR 1050
    FFQLLDRKPP INVYSEAGEK WDNFQGKIDF IDCKFTYPSR PDIQVLNGLS 1100
    VSVNPGQTLA FVGSSGCGKS TSIQLLERFY DPDQGTVMID GHDSKKVNIQ 1150
    FLRSNIGIVS QEPVLFDCSI MDNIKYGDNT KEISVERAIA AAKQAQLHDF 1200
    VMSLPEKYET NVGIQGSQLS RGEKQRIAIA RAIVRDPKIL LLDEATSALD 1250
    TESEKTVQTA LDKAREGRTC IVIAHRLSTI QNSDIIAVVS QGVVIEKGTH 1300
    EKLMAQKGAY YKLVITGAPI S 1321
    Length:1,321
    Mass (Da):146,258
    Last modified:August 1, 1998 - v1
    Checksum:i5443F4EF7B9FB1F6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U69487 mRNA. Translation: AAC40084.1.
    PIRiT42842.
    RefSeqiNP_113948.1. NM_031760.1.
    UniGeneiRn.14539.

    Genome annotation databases

    GeneIDi83569.
    KEGGirno:83569.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U69487 mRNA. Translation: AAC40084.1 .
    PIRi T42842.
    RefSeqi NP_113948.1. NM_031760.1.
    UniGenei Rn.14539.

    3D structure databases

    ProteinModelPortali O70127.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 249756. 1 interaction.
    IntActi O70127. 2 interactions.

    Chemistry

    BindingDBi O70127.
    ChEMBLi CHEMBL2073674.

    PTM databases

    PhosphoSitei O70127.

    Proteomic databases

    PRIDEi O70127.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 83569.
    KEGGi rno:83569.

    Organism-specific databases

    CTDi 8647.
    RGDi 619930. Abcb11.

    Phylogenomic databases

    HOVERGENi HBG080809.
    KOi K05664.
    PhylomeDBi O70127.

    Miscellaneous databases

    NextBioi 616061.
    PROi O70127.

    Gene expression databases

    Genevestigatori O70127.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR011527. ABC1_TM_dom.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR001140. ABC_transptr_TM_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00664. ABC_membrane. 2 hits.
    PF00005. ABC_tran. 2 hits.
    [Graphical view ]
    SMARTi SM00382. AAA. 2 hits.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    SSF90123. SSF90123. 3 hits.
    PROSITEi PS50929. ABC_TM1F. 2 hits.
    PS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The sister of P-glycoprotein represents the canalicular bile salt export pump of mammalian liver."
      Gerloff T., Stieger B., Hagenbuch B., Madon J., Landmann L., Roth J., Hofmann A.F., Meier P.J.
      J. Biol. Chem. 273:10046-10050(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Liver.
    2. "Identification of HAX-1 as a protein that binds bile salt export protein and regulates its abundance in the apical membrane of Madin-Darby canine kidney cells."
      Ortiz D.F., Moseley J., Calderon G., Swift A.L., Li S., Arias I.M.
      J. Biol. Chem. 279:32761-32770(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HAX1.

    Entry informationi

    Entry nameiABCBB_RAT
    AccessioniPrimary (citable) accession number: O70127
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 24, 2001
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3