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Protein

Vitamin B12-dependent ribonucleotide reductase

Gene

nrdJ

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactori

adenosylcob(III)alaminCuratedNote: 5'-deoxyadenosylcobalamine (coenzyme B12).Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei143SubstrateBy similarity1
Binding sitei188Substrate; via amide nitrogenBy similarity1
Active sitei364Proton acceptorBy similarity1
Active sitei366Cysteine radical intermediateBy similarity1
Active sitei368Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

Cobalamin, Cobalt, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Vitamin B12-dependent ribonucleotide reductase (EC:1.17.4.1)
Alternative name(s):
Ribonucleoside-diphosphate reductase NrdJ
Gene namesi
Name:nrdJ
Ordered Locus Names:SCO5805
ORF Names:SC4H2.26
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
Proteomesi
  • UP000001973 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002290291 – 967Vitamin B12-dependent ribonucleotide reductaseAdd BLAST967

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi160 ↔ 377Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiO69981.

Expressioni

Developmental stagei

Expressed during the exponential phase of growth.

Inductioni

Induced by NrdR.

Interactioni

Protein-protein interaction databases

STRINGi100226.SCO5805.

Structurei

3D structure databases

ProteinModelPortaliO69981.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni159 – 160Substrate bindingBy similarity2
Regioni364 – 368Substrate bindingBy similarity5
Regioni554 – 558Substrate bindingBy similarity5

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105BZH. Bacteria.
COG0209. LUCA.
HOGENOMiHOG000021771.
InParanoidiO69981.
KOiK00525.
OMAiMTGVSYA.
OrthoDBiPOG091H0FGZ.
PhylomeDBiO69981.

Family and domain databases

InterProiIPR013678. RNR_2_N.
IPR000788. RNR_lg_C.
IPR013344. RNR_NrdJ/NrdZ.
[Graphical view]
PfamiPF08471. Ribonuc_red_2_N. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02504. NrdJ_Z. 1 hit.

Sequencei

Sequence statusi: Complete.

O69981-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTETASGPAR SSRAKGTKAG KGLRVERVHT TPGVHPYDEV AWERRDVVMT
60 70 80 90 100
NWRDGSVNFE QRGVEFPEFW SVNAVNIVTS KYFRGAVGTP QREVSLKQLI
110 120 130 140 150
DRIVKTYRKA GEDNKYFASP ADAEIFEHEL AYALLHQIFS FNSPVWFNVG
160 170 180 190 200
TPQPQQVSAC FILSVDDSME SILDWYKEEG MIFKGGSGAG LNLSRIRSSK
210 220 230 240 250
ELLSSGGNAS GPVSFMRGAD ASAGTIKSGG ATRRAAKMVI LDVDHPDIED
260 270 280 290 300
FIQTKVKEEE KIRALRDAGF DMDLGGDDIT SVQYQNANNS VRVNDTFMKA
310 320 330 340 350
VQDGGKFGLT SRMTGEVIEE VDAKALFRKM AEAAWACADP GIQYDDTINA
360 370 380 390 400
WHTCPESGRI NGSNPCSEYM HLDNTSCNLA SLNLMKFLKD DGKGNQSFDA
410 420 430 440 450
ERFSKVVELV ITAMDISICF ADFPTQKIGE NTRAFRQLGI GYANLGALLM
460 470 480 490 500
ATGHAYDSDG GRALAGAITS LMTGTSYRRS AELAAIVGPY DGYARNAKPH
510 520 530 540 550
LRVMKQHSDE NAKAVRMDDL DTPIWAAATE AWQDVLRLGE KNGFRNSQAS
560 570 580 590 600
VIAPTGTIGL AMSCDTTGLE PDLALVKFKK LVGGGSMQIV NGTVPQALRR
610 620 630 640 650
LGYQEEQIEA IVAHIAENGN VIDAPGLKPE HYEVFDCAMG ERSISAMGHV
660 670 680 690 700
RMMAAIQPWI SGALSKTVNL PESATVEDVE EVYFEAWKMG VKALAIYRDN
710 720 730 740 750
CKVGQPLSAK TKTVKDTEKA EITEKTEAAI RETVEKVVEY RPVRKRLPKG
760 770 780 790 800
RPGITTSFTV GGAEGYMTAN SYPDDGLGEV FLKMSKQGST LAGMMDAFSI
810 820 830 840 850
AVSVGLQYGV PLETYVSKFT NMRFEPAGMT DDPDVRMAQS IVDYIFRRLA
860 870 880 890 900
LDFLPFETRS ALGIHSAEER QRHLETGSYE PSDDELDVEG LAQSAPRAQE
910 920 930 940 950
LVAVATPKAE AEAAKPAPQQ AHTSAELVEM QLGIQADAPL CFSCGTKMQR
960
AGSCYICEGC GSTSGCS
Length:967
Mass (Da):105,285
Last modified:August 1, 1998 - v1
Checksum:i1A6522C328673A29
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939125 Genomic DNA. Translation: CAA18341.1.
AM039891 Genomic RNA. Translation: CAJ01782.1.
PIRiT35125.
RefSeqiNP_629929.1. NC_003888.3.
WP_011030467.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAA18341; CAA18341; CAA18341.
GeneIDi1101247.
KEGGisco:SCO5805.
PATRICi23741500. VBIStrCoe124346_5897.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939125 Genomic DNA. Translation: CAA18341.1.
AM039891 Genomic RNA. Translation: CAJ01782.1.
PIRiT35125.
RefSeqiNP_629929.1. NC_003888.3.
WP_011030467.1. NC_003888.3.

3D structure databases

ProteinModelPortaliO69981.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi100226.SCO5805.

Proteomic databases

PRIDEiO69981.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAA18341; CAA18341; CAA18341.
GeneIDi1101247.
KEGGisco:SCO5805.
PATRICi23741500. VBIStrCoe124346_5897.

Phylogenomic databases

eggNOGiENOG4105BZH. Bacteria.
COG0209. LUCA.
HOGENOMiHOG000021771.
InParanoidiO69981.
KOiK00525.
OMAiMTGVSYA.
OrthoDBiPOG091H0FGZ.
PhylomeDBiO69981.

Family and domain databases

InterProiIPR013678. RNR_2_N.
IPR000788. RNR_lg_C.
IPR013344. RNR_NrdJ/NrdZ.
[Graphical view]
PfamiPF08471. Ribonuc_red_2_N. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02504. NrdJ_Z. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNRDJ_STRCO
AccessioniPrimary (citable) accession number: O69981
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Can grow normally using either of its two RNR systems.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.