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O69981

- NRDJ_STRCO

UniProt

O69981 - NRDJ_STRCO

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Protein

Vitamin B12-dependent ribonucleotide reductase

Gene

nrdJ

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactori

adenosylcob(III)alaminCuratedNote: 5'-deoxyadenosylcobalamine (coenzyme B12).Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei143 – 1431SubstrateBy similarity
Binding sitei188 – 1881Substrate; via amide nitrogenBy similarity
Active sitei364 – 3641Proton acceptorBy similarity
Active sitei366 – 3661Cysteine radical intermediateBy similarity
Active sitei368 – 3681Proton acceptorBy similarity

GO - Molecular functioni

  1. cobalamin binding Source: UniProtKB-KW
  2. cobalt ion binding Source: InterPro
  3. nucleotide binding Source: UniProtKB-KW
  4. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

Cobalamin, Cobalt, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Vitamin B12-dependent ribonucleotide reductase (EC:1.17.4.1)
Alternative name(s):
Ribonucleoside-diphosphate reductase NrdJ
Gene namesi
Name:nrdJ
Ordered Locus Names:SCO5805
ORF Names:SC4H2.26
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 967967Vitamin B12-dependent ribonucleotide reductasePRO_0000229029Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi160 ↔ 377Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiO69981.

Expressioni

Developmental stagei

Expressed during the exponential phase of growth.

Inductioni

Induced by NrdR.

Interactioni

Protein-protein interaction databases

STRINGi100226.SCO5805.

Structurei

3D structure databases

ProteinModelPortaliO69981.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni159 – 1602Substrate bindingBy similarity
Regioni364 – 3685Substrate bindingBy similarity
Regioni554 – 5585Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0209.
HOGENOMiHOG000021771.
InParanoidiO69981.
KOiK00525.
OMAiTAFDIKF.
OrthoDBiEOG6H4K5F.
PhylomeDBiO69981.

Family and domain databases

InterProiIPR013678. RNR_2_N.
IPR000788. RNR_lg_C.
IPR013344. RNR_NrdJ/NrdZ.
IPR024434. TSCPD_dom.
[Graphical view]
PfamiPF08471. Ribonuc_red_2_N. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF12637. TSCPD. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02504. NrdJ_Z. 1 hit.

Sequencei

Sequence statusi: Complete.

O69981-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTETASGPAR SSRAKGTKAG KGLRVERVHT TPGVHPYDEV AWERRDVVMT
60 70 80 90 100
NWRDGSVNFE QRGVEFPEFW SVNAVNIVTS KYFRGAVGTP QREVSLKQLI
110 120 130 140 150
DRIVKTYRKA GEDNKYFASP ADAEIFEHEL AYALLHQIFS FNSPVWFNVG
160 170 180 190 200
TPQPQQVSAC FILSVDDSME SILDWYKEEG MIFKGGSGAG LNLSRIRSSK
210 220 230 240 250
ELLSSGGNAS GPVSFMRGAD ASAGTIKSGG ATRRAAKMVI LDVDHPDIED
260 270 280 290 300
FIQTKVKEEE KIRALRDAGF DMDLGGDDIT SVQYQNANNS VRVNDTFMKA
310 320 330 340 350
VQDGGKFGLT SRMTGEVIEE VDAKALFRKM AEAAWACADP GIQYDDTINA
360 370 380 390 400
WHTCPESGRI NGSNPCSEYM HLDNTSCNLA SLNLMKFLKD DGKGNQSFDA
410 420 430 440 450
ERFSKVVELV ITAMDISICF ADFPTQKIGE NTRAFRQLGI GYANLGALLM
460 470 480 490 500
ATGHAYDSDG GRALAGAITS LMTGTSYRRS AELAAIVGPY DGYARNAKPH
510 520 530 540 550
LRVMKQHSDE NAKAVRMDDL DTPIWAAATE AWQDVLRLGE KNGFRNSQAS
560 570 580 590 600
VIAPTGTIGL AMSCDTTGLE PDLALVKFKK LVGGGSMQIV NGTVPQALRR
610 620 630 640 650
LGYQEEQIEA IVAHIAENGN VIDAPGLKPE HYEVFDCAMG ERSISAMGHV
660 670 680 690 700
RMMAAIQPWI SGALSKTVNL PESATVEDVE EVYFEAWKMG VKALAIYRDN
710 720 730 740 750
CKVGQPLSAK TKTVKDTEKA EITEKTEAAI RETVEKVVEY RPVRKRLPKG
760 770 780 790 800
RPGITTSFTV GGAEGYMTAN SYPDDGLGEV FLKMSKQGST LAGMMDAFSI
810 820 830 840 850
AVSVGLQYGV PLETYVSKFT NMRFEPAGMT DDPDVRMAQS IVDYIFRRLA
860 870 880 890 900
LDFLPFETRS ALGIHSAEER QRHLETGSYE PSDDELDVEG LAQSAPRAQE
910 920 930 940 950
LVAVATPKAE AEAAKPAPQQ AHTSAELVEM QLGIQADAPL CFSCGTKMQR
960
AGSCYICEGC GSTSGCS
Length:967
Mass (Da):105,285
Last modified:August 1, 1998 - v1
Checksum:i1A6522C328673A29
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939125 Genomic DNA. Translation: CAA18341.1.
AM039891 Genomic RNA. Translation: CAJ01782.1.
PIRiT35125.
RefSeqiNP_629929.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAA18341; CAA18341; CAA18341.
GeneIDi1101247.
KEGGisco:SCO5805.
PATRICi23741500. VBIStrCoe124346_5897.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939125 Genomic DNA. Translation: CAA18341.1 .
AM039891 Genomic RNA. Translation: CAJ01782.1 .
PIRi T35125.
RefSeqi NP_629929.1. NC_003888.3.

3D structure databases

ProteinModelPortali O69981.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 100226.SCO5805.

Proteomic databases

PRIDEi O69981.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAA18341 ; CAA18341 ; CAA18341 .
GeneIDi 1101247.
KEGGi sco:SCO5805.
PATRICi 23741500. VBIStrCoe124346_5897.

Phylogenomic databases

eggNOGi COG0209.
HOGENOMi HOG000021771.
InParanoidi O69981.
KOi K00525.
OMAi TAFDIKF.
OrthoDBi EOG6H4K5F.
PhylomeDBi O69981.

Family and domain databases

InterProi IPR013678. RNR_2_N.
IPR000788. RNR_lg_C.
IPR013344. RNR_NrdJ/NrdZ.
IPR024434. TSCPD_dom.
[Graphical view ]
Pfami PF08471. Ribonuc_red_2_N. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF12637. TSCPD. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
TIGRFAMsi TIGR02504. NrdJ_Z. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.
  2. "Streptomyces spp. contain class Ia and class II ribonucleotide reductases: expression analysis of the genes in vegetative growth."
    Borovok I., Kreisberg-Zakarin R., Yanko M., Schreiber R., Myslovati M., Aaslund F., Holmgren A., Cohen G., Aharonowitz Y.
    Microbiology 148:391-404(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.
  3. "Alternative oxygen-dependent and oxygen-independent ribonucleotide reductases in Streptomyces: cross-regulation and physiological role in response to oxygen limitation."
    Borovok I., Gorovitz B., Yanku M., Schreiber R., Gust B., Chater K., Aharonowitz Y., Cohen G.
    Mol. Microbiol. 54:1022-1035(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], REGULATION.
    Strain: ATCC BAA-471 / A3(2) / M145.

Entry informationi

Entry nameiNRDJ_STRCO
AccessioniPrimary (citable) accession number: O69981
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: August 1, 1998
Last modified: November 26, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Can grow normally using either of its two RNR systems.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3