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O69981

- NRDJ_STRCO

UniProt

O69981 - NRDJ_STRCO

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Protein
Vitamin B12-dependent ribonucleotide reductase
Gene
nrdJ, SCO5805, SC4H2.26
Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactori

5'-deoxyadenosylcobalamine (coenzyme B12) Inferred.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei143 – 1431Substrate By similarity
Binding sitei188 – 1881Substrate; via amide nitrogen By similarity
Active sitei364 – 3641Proton acceptor By similarity
Active sitei366 – 3661Cysteine radical intermediate By similarity
Active sitei368 – 3681Proton acceptor By similarity

GO - Molecular functioni

  1. cobalamin binding Source: UniProtKB-KW
  2. cobalt ion binding Source: InterPro
  3. nucleotide binding Source: UniProtKB-KW
  4. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

Cobalamin, Cobalt, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Vitamin B12-dependent ribonucleotide reductase (EC:1.17.4.1)
Alternative name(s):
Ribonucleoside-diphosphate reductase NrdJ
Gene namesi
Name:nrdJ
Ordered Locus Names:SCO5805
ORF Names:SC4H2.26
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 967967Vitamin B12-dependent ribonucleotide reductase
PRO_0000229029Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi160 ↔ 377Redox-active By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiO69981.

Expressioni

Developmental stagei

Expressed during the exponential phase of growth.

Inductioni

Induced by NrdR.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi100226.SCO5805.

Structurei

3D structure databases

ProteinModelPortaliO69981.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni159 – 1602Substrate binding By similarity
Regioni364 – 3685Substrate binding By similarity
Regioni554 – 5585Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0209.
HOGENOMiHOG000021771.
KOiK00525.
OMAiTAFDIKF.
OrthoDBiEOG6H4K5F.
PhylomeDBiO69981.

Family and domain databases

InterProiIPR013678. RNR_2_N.
IPR000788. RNR_lg_C.
IPR013344. RNR_NrdJ/NrdZ.
IPR024434. TSCPD_dom.
[Graphical view]
PfamiPF08471. Ribonuc_red_2_N. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF12637. TSCPD. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02504. NrdJ_Z. 1 hit.

Sequencei

Sequence statusi: Complete.

O69981-1 [UniParc]FASTAAdd to Basket

« Hide

MTETASGPAR SSRAKGTKAG KGLRVERVHT TPGVHPYDEV AWERRDVVMT    50
NWRDGSVNFE QRGVEFPEFW SVNAVNIVTS KYFRGAVGTP QREVSLKQLI 100
DRIVKTYRKA GEDNKYFASP ADAEIFEHEL AYALLHQIFS FNSPVWFNVG 150
TPQPQQVSAC FILSVDDSME SILDWYKEEG MIFKGGSGAG LNLSRIRSSK 200
ELLSSGGNAS GPVSFMRGAD ASAGTIKSGG ATRRAAKMVI LDVDHPDIED 250
FIQTKVKEEE KIRALRDAGF DMDLGGDDIT SVQYQNANNS VRVNDTFMKA 300
VQDGGKFGLT SRMTGEVIEE VDAKALFRKM AEAAWACADP GIQYDDTINA 350
WHTCPESGRI NGSNPCSEYM HLDNTSCNLA SLNLMKFLKD DGKGNQSFDA 400
ERFSKVVELV ITAMDISICF ADFPTQKIGE NTRAFRQLGI GYANLGALLM 450
ATGHAYDSDG GRALAGAITS LMTGTSYRRS AELAAIVGPY DGYARNAKPH 500
LRVMKQHSDE NAKAVRMDDL DTPIWAAATE AWQDVLRLGE KNGFRNSQAS 550
VIAPTGTIGL AMSCDTTGLE PDLALVKFKK LVGGGSMQIV NGTVPQALRR 600
LGYQEEQIEA IVAHIAENGN VIDAPGLKPE HYEVFDCAMG ERSISAMGHV 650
RMMAAIQPWI SGALSKTVNL PESATVEDVE EVYFEAWKMG VKALAIYRDN 700
CKVGQPLSAK TKTVKDTEKA EITEKTEAAI RETVEKVVEY RPVRKRLPKG 750
RPGITTSFTV GGAEGYMTAN SYPDDGLGEV FLKMSKQGST LAGMMDAFSI 800
AVSVGLQYGV PLETYVSKFT NMRFEPAGMT DDPDVRMAQS IVDYIFRRLA 850
LDFLPFETRS ALGIHSAEER QRHLETGSYE PSDDELDVEG LAQSAPRAQE 900
LVAVATPKAE AEAAKPAPQQ AHTSAELVEM QLGIQADAPL CFSCGTKMQR 950
AGSCYICEGC GSTSGCS 967
Length:967
Mass (Da):105,285
Last modified:August 1, 1998 - v1
Checksum:i1A6522C328673A29
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL939125 Genomic DNA. Translation: CAA18341.1.
AM039891 Genomic RNA. Translation: CAJ01782.1.
PIRiT35125.
RefSeqiNP_629929.1. NC_003888.3.
WP_011030467.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAA18341; CAA18341; CAA18341.
GeneIDi1101247.
KEGGisco:SCO5805.
PATRICi23741500. VBIStrCoe124346_5897.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL939125 Genomic DNA. Translation: CAA18341.1 .
AM039891 Genomic RNA. Translation: CAJ01782.1 .
PIRi T35125.
RefSeqi NP_629929.1. NC_003888.3.
WP_011030467.1. NC_003888.3.

3D structure databases

ProteinModelPortali O69981.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 100226.SCO5805.

Proteomic databases

PRIDEi O69981.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAA18341 ; CAA18341 ; CAA18341 .
GeneIDi 1101247.
KEGGi sco:SCO5805.
PATRICi 23741500. VBIStrCoe124346_5897.

Phylogenomic databases

eggNOGi COG0209.
HOGENOMi HOG000021771.
KOi K00525.
OMAi TAFDIKF.
OrthoDBi EOG6H4K5F.
PhylomeDBi O69981.

Family and domain databases

InterProi IPR013678. RNR_2_N.
IPR000788. RNR_lg_C.
IPR013344. RNR_NrdJ/NrdZ.
IPR024434. TSCPD_dom.
[Graphical view ]
Pfami PF08471. Ribonuc_red_2_N. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF12637. TSCPD. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
TIGRFAMsi TIGR02504. NrdJ_Z. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.
  2. "Streptomyces spp. contain class Ia and class II ribonucleotide reductases: expression analysis of the genes in vegetative growth."
    Borovok I., Kreisberg-Zakarin R., Yanko M., Schreiber R., Myslovati M., Aaslund F., Holmgren A., Cohen G., Aharonowitz Y.
    Microbiology 148:391-404(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.
  3. "Alternative oxygen-dependent and oxygen-independent ribonucleotide reductases in Streptomyces: cross-regulation and physiological role in response to oxygen limitation."
    Borovok I., Gorovitz B., Yanku M., Schreiber R., Gust B., Chater K., Aharonowitz Y., Cohen G.
    Mol. Microbiol. 54:1022-1035(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], REGULATION.
    Strain: ATCC BAA-471 / A3(2) / M145.

Entry informationi

Entry nameiNRDJ_STRCO
AccessioniPrimary (citable) accession number: O69981
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: August 1, 1998
Last modified: September 3, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Can grow normally using either of its two RNR systems.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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