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O69981

- NRDJ_STRCO

UniProt

O69981 - NRDJ_STRCO

Protein

Vitamin B12-dependent ribonucleotide reductase

Gene

nrdJ

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen.

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

    Cofactori

    5'-deoxyadenosylcobalamine (coenzyme B12).Curated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei143 – 1431SubstrateBy similarity
    Binding sitei188 – 1881Substrate; via amide nitrogenBy similarity
    Active sitei364 – 3641Proton acceptorBy similarity
    Active sitei366 – 3661Cysteine radical intermediateBy similarity
    Active sitei368 – 3681Proton acceptorBy similarity

    GO - Molecular functioni

    1. cobalamin binding Source: UniProtKB-KW
    2. cobalt ion binding Source: InterPro
    3. nucleotide binding Source: UniProtKB-KW
    4. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA synthesis

    Keywords - Ligandi

    Cobalamin, Cobalt, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vitamin B12-dependent ribonucleotide reductase (EC:1.17.4.1)
    Alternative name(s):
    Ribonucleoside-diphosphate reductase NrdJ
    Gene namesi
    Name:nrdJ
    Ordered Locus Names:SCO5805
    ORF Names:SC4H2.26
    OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
    Taxonomic identifieri100226 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
    ProteomesiUP000001973: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 967967Vitamin B12-dependent ribonucleotide reductasePRO_0000229029Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi160 ↔ 377Redox-activeBy similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PRIDEiO69981.

    Expressioni

    Developmental stagei

    Expressed during the exponential phase of growth.

    Inductioni

    Induced by NrdR.

    Interactioni

    Protein-protein interaction databases

    STRINGi100226.SCO5805.

    Structurei

    3D structure databases

    ProteinModelPortaliO69981.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni159 – 1602Substrate bindingBy similarity
    Regioni364 – 3685Substrate bindingBy similarity
    Regioni554 – 5585Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0209.
    HOGENOMiHOG000021771.
    KOiK00525.
    OMAiTAFDIKF.
    OrthoDBiEOG6H4K5F.
    PhylomeDBiO69981.

    Family and domain databases

    InterProiIPR013678. RNR_2_N.
    IPR000788. RNR_lg_C.
    IPR013344. RNR_NrdJ/NrdZ.
    IPR024434. TSCPD_dom.
    [Graphical view]
    PfamiPF08471. Ribonuc_red_2_N. 1 hit.
    PF02867. Ribonuc_red_lgC. 1 hit.
    PF12637. TSCPD. 1 hit.
    [Graphical view]
    PRINTSiPR01183. RIBORDTASEM1.
    TIGRFAMsiTIGR02504. NrdJ_Z. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O69981-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTETASGPAR SSRAKGTKAG KGLRVERVHT TPGVHPYDEV AWERRDVVMT    50
    NWRDGSVNFE QRGVEFPEFW SVNAVNIVTS KYFRGAVGTP QREVSLKQLI 100
    DRIVKTYRKA GEDNKYFASP ADAEIFEHEL AYALLHQIFS FNSPVWFNVG 150
    TPQPQQVSAC FILSVDDSME SILDWYKEEG MIFKGGSGAG LNLSRIRSSK 200
    ELLSSGGNAS GPVSFMRGAD ASAGTIKSGG ATRRAAKMVI LDVDHPDIED 250
    FIQTKVKEEE KIRALRDAGF DMDLGGDDIT SVQYQNANNS VRVNDTFMKA 300
    VQDGGKFGLT SRMTGEVIEE VDAKALFRKM AEAAWACADP GIQYDDTINA 350
    WHTCPESGRI NGSNPCSEYM HLDNTSCNLA SLNLMKFLKD DGKGNQSFDA 400
    ERFSKVVELV ITAMDISICF ADFPTQKIGE NTRAFRQLGI GYANLGALLM 450
    ATGHAYDSDG GRALAGAITS LMTGTSYRRS AELAAIVGPY DGYARNAKPH 500
    LRVMKQHSDE NAKAVRMDDL DTPIWAAATE AWQDVLRLGE KNGFRNSQAS 550
    VIAPTGTIGL AMSCDTTGLE PDLALVKFKK LVGGGSMQIV NGTVPQALRR 600
    LGYQEEQIEA IVAHIAENGN VIDAPGLKPE HYEVFDCAMG ERSISAMGHV 650
    RMMAAIQPWI SGALSKTVNL PESATVEDVE EVYFEAWKMG VKALAIYRDN 700
    CKVGQPLSAK TKTVKDTEKA EITEKTEAAI RETVEKVVEY RPVRKRLPKG 750
    RPGITTSFTV GGAEGYMTAN SYPDDGLGEV FLKMSKQGST LAGMMDAFSI 800
    AVSVGLQYGV PLETYVSKFT NMRFEPAGMT DDPDVRMAQS IVDYIFRRLA 850
    LDFLPFETRS ALGIHSAEER QRHLETGSYE PSDDELDVEG LAQSAPRAQE 900
    LVAVATPKAE AEAAKPAPQQ AHTSAELVEM QLGIQADAPL CFSCGTKMQR 950
    AGSCYICEGC GSTSGCS 967
    Length:967
    Mass (Da):105,285
    Last modified:August 1, 1998 - v1
    Checksum:i1A6522C328673A29
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL939125 Genomic DNA. Translation: CAA18341.1.
    AM039891 Genomic RNA. Translation: CAJ01782.1.
    PIRiT35125.
    RefSeqiNP_629929.1. NC_003888.3.
    WP_011030467.1. NC_003888.3.

    Genome annotation databases

    EnsemblBacteriaiCAA18341; CAA18341; CAA18341.
    GeneIDi1101247.
    KEGGisco:SCO5805.
    PATRICi23741500. VBIStrCoe124346_5897.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL939125 Genomic DNA. Translation: CAA18341.1 .
    AM039891 Genomic RNA. Translation: CAJ01782.1 .
    PIRi T35125.
    RefSeqi NP_629929.1. NC_003888.3.
    WP_011030467.1. NC_003888.3.

    3D structure databases

    ProteinModelPortali O69981.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 100226.SCO5805.

    Proteomic databases

    PRIDEi O69981.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAA18341 ; CAA18341 ; CAA18341 .
    GeneIDi 1101247.
    KEGGi sco:SCO5805.
    PATRICi 23741500. VBIStrCoe124346_5897.

    Phylogenomic databases

    eggNOGi COG0209.
    HOGENOMi HOG000021771.
    KOi K00525.
    OMAi TAFDIKF.
    OrthoDBi EOG6H4K5F.
    PhylomeDBi O69981.

    Family and domain databases

    InterProi IPR013678. RNR_2_N.
    IPR000788. RNR_lg_C.
    IPR013344. RNR_NrdJ/NrdZ.
    IPR024434. TSCPD_dom.
    [Graphical view ]
    Pfami PF08471. Ribonuc_red_2_N. 1 hit.
    PF02867. Ribonuc_red_lgC. 1 hit.
    PF12637. TSCPD. 1 hit.
    [Graphical view ]
    PRINTSi PR01183. RIBORDTASEM1.
    TIGRFAMsi TIGR02504. NrdJ_Z. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-471 / A3(2) / M145.
    2. "Streptomyces spp. contain class Ia and class II ribonucleotide reductases: expression analysis of the genes in vegetative growth."
      Borovok I., Kreisberg-Zakarin R., Yanko M., Schreiber R., Myslovati M., Aaslund F., Holmgren A., Cohen G., Aharonowitz Y.
      Microbiology 148:391-404(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC BAA-471 / A3(2) / M145.
    3. "Alternative oxygen-dependent and oxygen-independent ribonucleotide reductases in Streptomyces: cross-regulation and physiological role in response to oxygen limitation."
      Borovok I., Gorovitz B., Yanku M., Schreiber R., Gust B., Chater K., Aharonowitz Y., Cohen G.
      Mol. Microbiol. 54:1022-1035(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], REGULATION.
      Strain: ATCC BAA-471 / A3(2) / M145.

    Entry informationi

    Entry nameiNRDJ_STRCO
    AccessioniPrimary (citable) accession number: O69981
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 21, 2006
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Can grow normally using either of its two RNR systems.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3