Reviewed, UniProtKB/Swiss-Prot O69980 (NRDR_STRCO)

Names and origin

Protein names

Recommended name:
    Transcriptional repressor nrdR

Gene names

Name:	nrdR
Ordered Locus Names:	SCO5804
ORF Names:	SC4H2.25

Organism

Streptomyces coelicolor [Complete proteome] [HAMAP]

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	182 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Negatively regulates transcription of nrdRJ (class II RNR genes) and nrdABS operon (class I RNR genes) by binding to NrdR-boxes which are proximal to or overlap with the promoter regions of class II and class Ia RNR operons, respectively. Ref.3 Ref.4 Ref.5
Cofactor	Binds 1 zinc ion. Ref.5
Domain	The ATP-cone domain may function to recruit ATP/dATP to elicit a conformational change in nrdR that is necessary for binding. HAMAP MF_00440
Disruption phenotype	Cells upregulate expression of nrdRJ and nrdABS trasncription. A mutant in which amino acids 1 to 41 are deleted is unable to bind zinc and also unable to bind the nrdABS and nrdRJ promoters. A mutant in which amino acids 151 to 182 are deleted is able to bind to both promoters to the same extent as the wild-type. Functional zinc-finger and ATP-cone domain are required for binding to the promoter regions of both sets of RNR genes. Ref.3
Miscellaneous	Seems to be allosterically activated by ATP/dATP. HAMAP MF_00440
Sequence similarities	Belongs to the nrdR family. Contains 1 ATP-cone domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Domain	Zinc-finger
Ligand	ATP-binding DNA-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Repressor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW transcription repressor activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 182	182	Transcriptional repressor nrdR HAMAP MF_00440		PRO_0000182365
Regions
	Domain	46 – 136	91	ATP-cone
	Zinc finger	3 – 34	32	HAMAP MF_00440
Experimental info
	Mutagenesis	3	1	C → A: 7-fold reduction in the amount of zinc bound. No binding to nrdABS and nrdRJ promoters. Ref.5
	Mutagenesis	50 – 51	2	KR → NG: Loss of ATP/dATP binding. Weak binding to nrdABS and nrdRJ promoters.

Sequences

	Sequence		Length	Mass (Da)
	O69980-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 4AC39D1114B9567A	FASTA	182	19,719
	10 20 30 40 50 60 MHCPFCRHPD SRVVDSRTTD DGTSIRRRRQ CPDCSRRFTT VETCSLMVVK RSGVTEPFSR 70 80 90 100 110 120 TKVINGVRKA CQGRPVTEDA LAQLGQRVEE AVRATGSAEL TTHDVGLAIL GPLQELDLVA 130 140 150 160 170 180 YLRFASVYRA FDSLEDFEAA IAELRETTGH PGEEDDTGAG SQENDRGPTG AGQVPEPAGA AD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Borovok I. Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.
[3]	"Alternative oxygen-dependent and oxygen-independent ribonucleotide reductases in Streptomyces: cross-regulation and physiological role in response to oxygen limitation." Borovok I., Gorovitz B., Yanku M., Schreiber R., Gust B., Chater K., Aharonowitz Y., Cohen G. Mol. Microbiol. 54:1022-1035(2004) [PubMed: 15522084] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE. Strain: ATCC BAA-471 / A3(2) / M145.
[4]	"Identification of a bacterial regulatory system for ribonucleotide reductases by phylogenetic profiling." Rodionov D.A., Gelfand M.S. Trends Genet. 21:385-389(2005) [PubMed: 15949864] [Abstract] Cited for: FUNCTION.
[5]	"The Streptomyces NrdR transcriptional regulator is a Zn ribbon/ATP cone protein that binds to the promoter regions of class Ia and class II ribonucleotide reductase operons." Grinberg I., Shteinberg T., Gorovitz B., Aharonowitz Y., Cohen G., Borovok I. J. Bacteriol. 188:7635-7644(2006) [PubMed: 16950922] [Abstract] Cited for: FUNCTION, ZINC-FINGER, COFACTOR, PROTEIN REGULATION, MUTAGENESIS OF CYS-3 AND 50-LYS-ARG-51. Strain: ATCC BAA-471 / A3(2) / M145.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM039891 Genomic RNA. Translation: CAJ01781.1. AL939125 Genomic DNA. Translation: CAA18340.1.
PIR	T35124.
RefSeq	NP_629928.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1101246.
KEGG	sco:SCO5804.
NMPDR	fig|100226.1.peg.5753.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG351755.
OMA	FASVYRD.
Enzyme and pathway databases
BioCyc	SCOE100226:SCO5804-MONOMER.
Family and domain databases
HAMAP	MF_00440. NrdR. [Tree]
InterPro	IPR005144. ATP-cone. IPR003796. RNR_NrdR-like. [Graphical view]
Pfam	PF03477. ATP-cone. 1 hit. [Graphical view]
TIGRFAMs	TIGR00244. RNR_NrdR-like. 1 hit.
PROSITE	PS51161. ATP_CONE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NRDR_STRCO

Accession

Primary (citable) accession number: O69980
Secondary accession number(s): Q4QZ21

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	August 1, 1998
Last modified:	February 9, 2010
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)