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O69873

- GLND_STRCO

UniProt

O69873 - GLND_STRCO

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Protein
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Gene
glnD, SCO5585, SC2E1.02
Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:SCO5585
ORF Names:SC2E1.02
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 835835Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
PRO_0000192770Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi100226.SCO5585.

Structurei

3D structure databases

ProteinModelPortaliO69873.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini432 – 549118HD
Add
BLAST
Domaini651 – 73686ACT 1
Add
BLAST
Domaini765 – 83571ACT 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 316316UridylyltransferaseUniRule annotation
Add
BLAST
Regioni317 – 650334Uridylyl-removingUniRule annotation
Add
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000021840.
KOiK00990.
OMAiLYCLWDM.
OrthoDBiEOG6CCH44.
PhylomeDBiO69873.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O69873-1 [UniParc]FASTAAdd to Basket

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MTDEAEDSGP GGYAAARLRL LTEGARSGPP RRRALAELTD GWLAGLFGAA    50
TEEHTGISLV AVGGYGRGEL SPRSDLDLLL LHDGRDDKAV AALADRLWYP 100
VWDLGIDLDH SVRTPQQARK TAGQDLKVHL GLLDARHLAG DLGLTASLRT 150
AVLADWRNQA PKRLPELRDL CAERAERQGE LQFLLEPDLK EARGGLRDAT 200
ALRAVAASWL ADAPREGLAE ARRRLLDVRD ALHLATGRAT DRLALQEQDQ 250
VAAELGLLDA DALLRQVYEA ARVISYAGDV TWREVGRVLR SRSVRPRLRA 300
MMNGRNGGKP VAERSPLAEG VVEQDGEAVL ARTARPERDP ALPLRAAAAA 350
AQAGLPLSRH AVRRLAATAR PLPTPWPAEA REQLVTLLGS GRPTVQVWEA 400
LEAEGLVTRL LPDWERVRCR PQRNAVHLWT VDRHLIETAV RAAGFTRRVH 450
RPDLLLIAAL LHDIGKGWPG DHSVAGETIA RDVAARIGFD GADTAVLATL 500
VRHHLLLVET ATRRDLDDPA TVRAVAQAVG TEHTLELLHA LTEADALATG 550
PAAWSSWRGS LVADLVKRVS GVLAGEPQPE AESAAPTAEQ ERLAVEAFRT 600
GGPVLALRAQ TEPPADSAPA PSSPSSPSFP SPLSSPSSPS SADGPEPLGV 650
ELLIAVPDQA GVLPAVAGVL AMHRLTVRTA ELRSVPLPDG VEGSVLLLDW 700
RVAAQYGSLP QAARLRADLV RALDGTLDIA ARLAERDAAH PRRRGVEPPP 750
PRVTVAPAAS RLATVIEVRA QDAPGLLFRL GRALEAAGVR VRSAHVSTLG 800
ANAVDAFYVT RGEGTPLPGD EAASVARGLE ESLRT 835
Length:835
Mass (Da):89,047
Last modified:August 1, 1998 - v1
Checksum:i551296D8BE77DE5D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL939124 Genomic DNA. Translation: CAA19377.1.
PIRiT34770.
RefSeqiNP_629719.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAA19377; CAA19377; CAA19377.
GeneIDi1101026.
KEGGisco:SCO5585.
PATRICi23741050. VBIStrCoe124346_5676.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL939124 Genomic DNA. Translation: CAA19377.1 .
PIRi T34770.
RefSeqi NP_629719.1. NC_003888.3.

3D structure databases

ProteinModelPortali O69873.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 100226.SCO5585.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAA19377 ; CAA19377 ; CAA19377 .
GeneIDi 1101026.
KEGGi sco:SCO5585.
PATRICi 23741050. VBIStrCoe124346_5676.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000021840.
KOi K00990.
OMAi LYCLWDM.
OrthoDBi EOG6CCH44.
PhylomeDBi O69873.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.

Entry informationi

Entry nameiGLND_STRCO
AccessioniPrimary (citable) accession number: O69873
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: May 14, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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