Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:SCO5585
ORF Names:SC2E1.02
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 835835Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000192770Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi100226.SCO5585.

Structurei

3D structure databases

ProteinModelPortaliO69873.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini432 – 549118HDUniRule annotationAdd
BLAST
Domaini651 – 73686ACT 1UniRule annotationAdd
BLAST
Domaini765 – 83571ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 316316UridylyltransferaseAdd
BLAST
Regioni317 – 650334Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000021840.
InParanoidiO69873.
KOiK00990.
OMAiELREPWP.
OrthoDBiEOG6CCH44.
PhylomeDBiO69873.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O69873-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDEAEDSGP GGYAAARLRL LTEGARSGPP RRRALAELTD GWLAGLFGAA
60 70 80 90 100
TEEHTGISLV AVGGYGRGEL SPRSDLDLLL LHDGRDDKAV AALADRLWYP
110 120 130 140 150
VWDLGIDLDH SVRTPQQARK TAGQDLKVHL GLLDARHLAG DLGLTASLRT
160 170 180 190 200
AVLADWRNQA PKRLPELRDL CAERAERQGE LQFLLEPDLK EARGGLRDAT
210 220 230 240 250
ALRAVAASWL ADAPREGLAE ARRRLLDVRD ALHLATGRAT DRLALQEQDQ
260 270 280 290 300
VAAELGLLDA DALLRQVYEA ARVISYAGDV TWREVGRVLR SRSVRPRLRA
310 320 330 340 350
MMNGRNGGKP VAERSPLAEG VVEQDGEAVL ARTARPERDP ALPLRAAAAA
360 370 380 390 400
AQAGLPLSRH AVRRLAATAR PLPTPWPAEA REQLVTLLGS GRPTVQVWEA
410 420 430 440 450
LEAEGLVTRL LPDWERVRCR PQRNAVHLWT VDRHLIETAV RAAGFTRRVH
460 470 480 490 500
RPDLLLIAAL LHDIGKGWPG DHSVAGETIA RDVAARIGFD GADTAVLATL
510 520 530 540 550
VRHHLLLVET ATRRDLDDPA TVRAVAQAVG TEHTLELLHA LTEADALATG
560 570 580 590 600
PAAWSSWRGS LVADLVKRVS GVLAGEPQPE AESAAPTAEQ ERLAVEAFRT
610 620 630 640 650
GGPVLALRAQ TEPPADSAPA PSSPSSPSFP SPLSSPSSPS SADGPEPLGV
660 670 680 690 700
ELLIAVPDQA GVLPAVAGVL AMHRLTVRTA ELRSVPLPDG VEGSVLLLDW
710 720 730 740 750
RVAAQYGSLP QAARLRADLV RALDGTLDIA ARLAERDAAH PRRRGVEPPP
760 770 780 790 800
PRVTVAPAAS RLATVIEVRA QDAPGLLFRL GRALEAAGVR VRSAHVSTLG
810 820 830
ANAVDAFYVT RGEGTPLPGD EAASVARGLE ESLRT
Length:835
Mass (Da):89,047
Last modified:July 31, 1998 - v1
Checksum:i551296D8BE77DE5D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939124 Genomic DNA. Translation: CAA19377.1.
PIRiT34770.
RefSeqiNP_629719.1. NC_003888.3.
WP_011030320.1. NC_003888.3.

Genome annotation databases

GeneIDi1101026.
KEGGisco:SCO5585.
PATRICi23741050. VBIStrCoe124346_5676.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939124 Genomic DNA. Translation: CAA19377.1.
PIRiT34770.
RefSeqiNP_629719.1. NC_003888.3.
WP_011030320.1. NC_003888.3.

3D structure databases

ProteinModelPortaliO69873.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi100226.SCO5585.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1101026.
KEGGisco:SCO5585.
PATRICi23741050. VBIStrCoe124346_5676.

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000021840.
InParanoidiO69873.
KOiK00990.
OMAiELREPWP.
OrthoDBiEOG6CCH44.
PhylomeDBiO69873.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.

Entry informationi

Entry nameiGLND_STRCO
AccessioniPrimary (citable) accession number: O69873
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2000
Last sequence update: July 31, 1998
Last modified: March 31, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.