O69782 (DAPAL_RHIML) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized DAPA-like lyase
EC=4.-.-.-

Gene names

Name:

dapAL

Encoded on

Plasmid pRmeGR4b

Organism

Rhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti)

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Upon expression in E.coli complements a dapA deletion mutation, but this may not be its physiological function.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Potential.
Induction	Expressed in vegetatively growing cells and in nodules. Ref.1
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Plasmid
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	lyase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Uncharacterized DAPA-like lyase		PRO_0000103142
Sites
	Active site	49	1	Charge relay system By similarity
	Active site	137	1	Proton donor By similarity
	Active site	165	1	Schiff-base intermediate with substrate By similarity
	Site	137	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	O69782 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 5B2F50DDF5625FB5	FASTA	300	32,297
	10 20 30 40 50 60 MALRTDWNGV FPAMVTPFRE NGSFDEASFK ALIELYISEG VKGVVVTGST GEWYSMSDAE 70 80 90 100 110 120 RATVWEVAVE ASSGRITVIA GTSAVGTREA LALTRTAKAV GVDGCMLLPP GGIFAARNEV 130 140 150 160 170 180 VNYFHTLAGV GLPIMVYNNP PRTGVNMDAD MVAEIAKSEE IVSFKDINRY LYAASEIIYR 190 200 210 220 230 240 VCDKLAVFTG LEPYASSVLP RGAVGVVSTI SNICAANMVS YYNAVISGDS ATAYKTQKLI 250 260 270 280 290 300 DQLYHFLPTL GAPAFVSVKA AMKLLGRPGG EIRLPHLPAN EALIGKLREE LRRLKMMTLN

References

[1]

"Characterization of the Sinorhizobium meliloti genes encoding a functional dihydrodipicolinate synthase (dapA) and dihydrodipicolinate reductase (dapB)." Garcia-Rodriguez F.M., Zekri S., Toro N. Arch. Microbiol. 173:438-444(2000) [PubMed: 10896225] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, EXPRESSION IN E.COLI. Strain: GR4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ222715 Genomic DNA. Translation: CAA10957.1.
3D structure databases
ProteinModelPortal	O69782.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR002220. Dihydrodipicolinate_synth-like. IPR020625. Dihydrodipicolinate_synth_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PIRSF	PIRSF001365. DHDPS. 1 hit.
PRINTS	PR00146. DHPICSNTHASE.
PROSITE	PS00665. DHDPS_1. False negative. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPAL_RHIML

Accession

Primary (citable) accession number: O69782

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program