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Protein

Uncharacterized DapA-like lyase

Gene

dapAL

Organism
Rhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Upon expression in E.coli complements a dapA deletion mutation, but this may not be its physiological function.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei49Charge relay systemBy similarity1
Active sitei137Proton donorBy similarity1
Sitei137Involved in proton transfer during cleavageBy similarity1
Active sitei165Schiff-base intermediate with substrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Schiff base

Names & Taxonomyi

Protein namesi
Recommended name:
Uncharacterized DapA-like lyase (EC:4.-.-.-)
Gene namesi
Name:dapAL
Encoded oniPlasmid pRmeGR4b0 Publication
OrganismiRhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri382 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001031421 – 300Uncharacterized DapA-like lyaseAdd BLAST300

Expressioni

Inductioni

Expressed in vegetatively growing cells and in nodules.1 Publication

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO69782.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DapA family.Curated

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002220. DapA-like.
IPR020625. Schiff_base-form_aldolases_AS.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.
SMARTiSM01130. DHDPS. 1 hit.
[Graphical view]
PROSITEiPS00666. DHDPS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O69782-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALRTDWNGV FPAMVTPFRE NGSFDEASFK ALIELYISEG VKGVVVTGST
60 70 80 90 100
GEWYSMSDAE RATVWEVAVE ASSGRITVIA GTSAVGTREA LALTRTAKAV
110 120 130 140 150
GVDGCMLLPP GGIFAARNEV VNYFHTLAGV GLPIMVYNNP PRTGVNMDAD
160 170 180 190 200
MVAEIAKSEE IVSFKDINRY LYAASEIIYR VCDKLAVFTG LEPYASSVLP
210 220 230 240 250
RGAVGVVSTI SNICAANMVS YYNAVISGDS ATAYKTQKLI DQLYHFLPTL
260 270 280 290 300
GAPAFVSVKA AMKLLGRPGG EIRLPHLPAN EALIGKLREE LRRLKMMTLN
Length:300
Mass (Da):32,297
Last modified:August 1, 1998 - v1
Checksum:i5B2F50DDF5625FB5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ222715 Genomic DNA. Translation: CAA10957.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ222715 Genomic DNA. Translation: CAA10957.1.

3D structure databases

ProteinModelPortaliO69782.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002220. DapA-like.
IPR020625. Schiff_base-form_aldolases_AS.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.
SMARTiSM01130. DHDPS. 1 hit.
[Graphical view]
PROSITEiPS00666. DHDPS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDAPAL_RHIML
AccessioniPrimary (citable) accession number: O69782
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: July 6, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.