Reviewed, UniProtKB/Swiss-Prot O69773 (AMPC_PROST)

Names and origin

Protein names

Recommended name:
    Beta-lactamase
    EC=3.5.2.6
Alternative name(s):
    Cephalosporinase

Gene names

Name:

ampC

Organism

Providencia stuartii

Taxonomic identifier

588 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Providencia

Protein attributes

Sequence length	384 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This protein is a serine beta-lactamase with a substrate specificity for cephalosporins.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Subcellular location	Periplasm By similarity.
Sequence similarities	Belongs to the class-C beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Cellular component	Periplasm
Domain	Signal
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 384	355	Beta-lactamase		PRO_0000016962
Regions
	Region	337 – 339	3	Substrate binding By similarity
Sites
	Active site	87	1	Acyl-ester intermediate By similarity
	Active site	172	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	O69773-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 8CCCAC7F9B1377E9	FASTA	384	43,366
	10 20 30 40 50 60 MDNSMKNIFR QGRLFIALSL AMTSISAFAL TQQEVDDIIK PLMKQEQIPG MSVAISVNGK 70 80 90 100 110 120 QAIYHYGVQS KQTQIPVSDR TLYEIGSLSK TFTATLATYA QIQGKLDFSQ SVSHYLPELK 130 140 150 160 170 180 GSAFDNVSVM NLATHTSGLS LFVPSDIKTN DQLMAYYQKW LPDNEVGQYR SYSNLGVGLL 190 200 210 220 230 240 GIVTAKQLNM PFSQAMEKLM LPSLGLKHTY IHVPKSQEKY YAQGYNKQNQ PVRLNLEILG 250 260 270 280 290 300 PEAYGLKSNA KDLIRYLEIN MQSIKVAKTW QEAIENTHTG VYLTDSFVQD MMWESYPWPV 310 320 330 340 350 360 SLSQLLQGNR DDMALKPQKV ELIKPAMAPE VRAYYNKTGS SNGFATYAIF IPEEKIAIVM 370 380 LSNKWIPIPQ RITATYQLLE KIER

References

[1]	"Cloning and sequencing of ampC and ampR genes from Providencia stuartii." Koeck J.L., Basmaciogullari S., Parzy D., Barnaud G., Teyssou R., Buisson Y., Philippon A., Arlet G.J. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: VDG 96.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y17315 Genomic DNA. Translation: CAA76739.1.
3D structure databases
SMR	O69773. Positions 32-381.
ModBase	Search...
Protein family/group databases
MEROPS	S12.006.
Enzyme and pathway databases
BRENDA	3.5.2.6. 96137.
Family and domain databases
InterPro	IPR012338. B-lactamase-typ_transpept_fold. IPR001586. Beta_lactam_C_AS. IPR001466. Beta_lactamase-related. [Graphical view]
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PROSITE	PS00336. BETA_LACTAMASE_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPC_PROST

Accession

Primary (citable) accession number: O69773

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	January 19, 2010
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)