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Protein

Probable sensor histidine kinase TcrY

Gene

tcrY

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system TcrY/TcrX. Activates TcrX by phosphorylation.2 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Cofactori

a divalent metal cation1 Publication

Kineticsi

  1. KM=10 mM for ATP1 Publication

    GO - Molecular functioni

    • ATPase activity Source: MTBBASE
    • ATP binding Source: UniProtKB-KW
    • calcium ion binding Source: MTBBASE
    • magnesium ion binding Source: MTBBASE
    • phosphorelay sensor kinase activity Source: InterPro
    • protein homodimerization activity Source: MTBBASE

    GO - Biological processi

    • protein autophosphorylation Source: MTBBASE
    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Two-component regulatory system

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMTBRV:RV3764C-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable sensor histidine kinase TcrY (EC:2.7.13.3)
    Gene namesi
    Name:tcrY
    Ordered Locus Names:Rv3764c
    OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
    Taxonomic identifieri83332 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    Proteomesi
    • UP000001584 Componenti: Chromosome

    Organism-specific databases

    TubercuListiRv3764c.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 153153ExtracellularSequence analysisAdd
    BLAST
    Transmembranei154 – 17421HelicalSequence analysisAdd
    BLAST
    Topological domaini175 – 475301CytoplasmicSequence analysisAdd
    BLAST

    GO - Cellular componenti

    • cytosol Source: MTBBASE
    • integral component of membrane Source: UniProtKB-KW
    • plasma membrane Source: MTBBASE
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Cells lacking TcrX and TcrY show an increase in virulence in mouse model of infection, with significantly shorter survival times.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 475475Probable sensor histidine kinase TcrYPRO_0000412200Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei256 – 2561Phosphohistidine; by autocatalysisPROSITE-ProRule annotation

    Post-translational modificationi

    Autophosphorylated.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiO69729.
    PRIDEiO69729.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    GO - Molecular functioni

    • protein homodimerization activity Source: MTBBASE

    Protein-protein interaction databases

    STRINGi83332.Rv3764c.

    Structurei

    3D structure databases

    ProteinModelPortaliO69729.
    SMRiO69729. Positions 181-464.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini176 – 23863HAMPPROSITE-ProRule annotationAdd
    BLAST
    Domaini253 – 466214Histidine kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 HAMP domain.PROSITE-ProRule annotation
    Contains 1 histidine kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG4105BZU. Bacteria.
    ENOG410XNMH. LUCA.
    HOGENOMiHOG000223177.
    InParanoidiO69729.
    KOiK02484.
    OMAiNRVESET.
    OrthoDBiEOG6G4VQG.
    PhylomeDBiO69729.

    Family and domain databases

    Gene3Di1.10.287.130. 1 hit.
    3.30.565.10. 1 hit.
    InterProiIPR003660. HAMP_dom.
    IPR003594. HATPase_C.
    IPR005467. His_kinase_dom.
    IPR003661. HisK_dim/P.
    IPR004358. Sig_transdc_His_kin-like_C.
    [Graphical view]
    PfamiPF00672. HAMP. 1 hit.
    PF02518. HATPase_c. 1 hit.
    PF00512. HisKA. 1 hit.
    [Graphical view]
    PRINTSiPR00344. BCTRLSENSOR.
    SMARTiSM00304. HAMP. 1 hit.
    SM00387. HATPase_c. 1 hit.
    SM00388. HisKA. 1 hit.
    [Graphical view]
    SUPFAMiSSF47384. SSF47384. 1 hit.
    SSF55874. SSF55874. 1 hit.
    PROSITEiPS50885. HAMP. 1 hit.
    PS50109. HIS_KIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O69729-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MGITAATEMA LRRHLVAQLD NQLGGTSYRS VLMYPEKMPR PPWRHETHNY
    60 70 80 90 100
    IRSGPGPRFL DAPGQPAGMV AAVVSDGTTV AAGYLTGSGS RAALTSTGRS
    110 120 130 140 150
    QLERIAGSRT PLTLDLDGLG RYRVLAAPSR NGHDVIVTGL SMGNVDATML
    160 170 180 190 200
    QMLIIFGIVT VIALVAATTA GIVIIKRALA PLRRVAQTAS EVVDLPLDRG
    210 220 230 240 250
    EVKLPVRVPE PDANPSTEVG QLGSALNRML DHIAAALSAR QASETCVRQF
    260 270 280 290 300
    VADASHELRT PLAAIRGYTE LTQRIGDDPE AVAHAMSRVA SETERITRLV
    310 320 330 340 350
    EDLLLLARLD SGRPLERGPV DMSRLAVDAV SDAHVAGPDH QWALDLPPEP
    360 370 380 390 400
    VVIPGDAARL HQVVTNLLAN ARVHTGPGTI VTTRLSTGPT HVVLQVIDNG
    410 420 430 440 450
    PGIPAALQSE VFERFARGDT SRSRQAGSTG LGLAIVSAVV KAHNGTITVS
    460 470
    SSPGYTEFAV RLPLDGWQPL ESSPR
    Length:475
    Mass (Da):50,344
    Last modified:August 1, 1998 - v1
    Checksum:i5CE7FC3E7D3A7BAF
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL123456 Genomic DNA. Translation: CCP46591.1.
    PIRiE70801.
    RefSeqiNP_218281.1. NC_000962.3.
    WP_010886181.1. NC_000962.3.

    Genome annotation databases

    EnsemblBacteriaiCCP46591; CCP46591; Rv3764c.
    GeneIDi886094.
    KEGGimtu:Rv3764c.
    mtv:RVBD_3764c.
    PATRICi18156977. VBIMycTub87468_4193.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL123456 Genomic DNA. Translation: CCP46591.1.
    PIRiE70801.
    RefSeqiNP_218281.1. NC_000962.3.
    WP_010886181.1. NC_000962.3.

    3D structure databases

    ProteinModelPortaliO69729.
    SMRiO69729. Positions 181-464.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi83332.Rv3764c.

    Proteomic databases

    PaxDbiO69729.
    PRIDEiO69729.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCCP46591; CCP46591; Rv3764c.
    GeneIDi886094.
    KEGGimtu:Rv3764c.
    mtv:RVBD_3764c.
    PATRICi18156977. VBIMycTub87468_4193.

    Organism-specific databases

    TubercuListiRv3764c.

    Phylogenomic databases

    eggNOGiENOG4105BZU. Bacteria.
    ENOG410XNMH. LUCA.
    HOGENOMiHOG000223177.
    InParanoidiO69729.
    KOiK02484.
    OMAiNRVESET.
    OrthoDBiEOG6G4VQG.
    PhylomeDBiO69729.

    Enzyme and pathway databases

    BioCyciMTBRV:RV3764C-MONOMER.

    Family and domain databases

    Gene3Di1.10.287.130. 1 hit.
    3.30.565.10. 1 hit.
    InterProiIPR003660. HAMP_dom.
    IPR003594. HATPase_C.
    IPR005467. His_kinase_dom.
    IPR003661. HisK_dim/P.
    IPR004358. Sig_transdc_His_kin-like_C.
    [Graphical view]
    PfamiPF00672. HAMP. 1 hit.
    PF02518. HATPase_c. 1 hit.
    PF00512. HisKA. 1 hit.
    [Graphical view]
    PRINTSiPR00344. BCTRLSENSOR.
    SMARTiSM00304. HAMP. 1 hit.
    SM00387. HATPase_c. 1 hit.
    SM00388. HisKA. 1 hit.
    [Graphical view]
    SUPFAMiSSF47384. SSF47384. 1 hit.
    SSF55874. SSF55874. 1 hit.
    PROSITEiPS50885. HAMP. 1 hit.
    PS50109. HIS_KIN. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25618 / H37Rv.
    2. "Deletion of two-component regulatory systems increases the virulence of Mycobacterium tuberculosis."
      Parish T., Smith D.A., Kendall S., Casali N., Bancroft G.J., Stoker N.G.
      Infect. Immun. 71:1134-1140(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, GENE NAME.
      Strain: ATCC 25618 / H37Rv.
    3. "Interaction analysis of TcrX/Y two component system from Mycobacterium tuberculosis."
      Bhattacharya M., Biswas A., Das A.K.
      Biochimie 92:263-272(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, AUTOPHOSPHORYLATION.
      Strain: ATCC 25618 / H37Rv.
    4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ATCC 25618 / H37Rv.

    Entry informationi

    Entry nameiTCRY_MYCTU
    AccessioniPrimary (citable) accession number: O69729
    Secondary accession number(s): L0TGH0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 27, 2011
    Last sequence update: August 1, 1998
    Last modified: November 11, 2015
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
      Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.