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Protein

HTH-type transcriptional regulator NmtR

Gene

nmtR

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Represses transcription of ctpJ/nmtA, by binding to its promoter region.1 Publication

Enzyme regulationi

Binding to DNA is inhibited by nickel and, to some extent, cobalt ions.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi91 – 911Nickel
Metal bindingi93 – 931Nickel
Metal bindingi104 – 1041Nickel
Metal bindingi107 – 1071Nickel

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi49 – 7224H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • regulation of fatty acid metabolic process Source: MTBBASE
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Nickel

Enzyme and pathway databases

BioCyciMTBRV:RV3744-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator NmtR
Gene namesi
Name:nmtR
Ordered Locus Names:Rv3744
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv3744.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi51 – 511D → A: Does not affect activity and regulation by metal ions. 1 Publication
Mutagenesisi91 – 911D → A: No change in activity. Lack of regulation by metal ions. 1 Publication
Mutagenesisi93 – 931H → R: No change in activity. Lack of regulation by metal ions. 1 Publication
Mutagenesisi104 – 1041H → R: No change in activity. Lack of regulation by metal ions. 1 Publication
Mutagenesisi107 – 1071H → R: No change in activity. Lack of regulation by metal ions. 1 Publication
Mutagenesisi109 – 1091H → R: No change in activity. Lack of regulation by metal ions. 1 Publication
Mutagenesisi114 – 1141D → A: Does not affect activity and regulation by metal ions. 1 Publication
Mutagenesisi116 – 1161H → R: No change in activity. Lack of regulation by metal ions. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 120120HTH-type transcriptional regulator NmtRPRO_0000419179Add
BLAST

Proteomic databases

PaxDbiO69711.
PRIDEiO69711.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi83332.Rv3744.

Structurei

3D structure databases

ProteinModelPortaliO69711.
SMRiO69711. Positions 2-120.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 10995HTH arsR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH arsR-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG41066UM. Bacteria.
ENOG41124TA. LUCA.
HOGENOMiHOG000144506.
InParanoidiO69711.
OMAiMIHHTRH.
OrthoDBiEOG6Z3KS4.
PhylomeDBiO69711.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR001845. HTH_ArsR_DNA-bd_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01022. HTH_5. 1 hit.
[Graphical view]
PRINTSiPR00778. HTHARSR.
SMARTiSM00418. HTH_ARSR. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS50987. HTH_ARSR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O69711-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGHGVEGRNR PSAPLDSQAA AQVASTLQAL ATPSRLMILT QLRNGPLPVT
60 70 80 90 100
DLAEAIGMEQ SAVSHQLRVL RNLGLVVGDR AGRSIVYSLY DTHVAQLLDE
110 120
AIYHSEHLHL GLSDRHPSAG
Length:120
Mass (Da):12,836
Last modified:July 5, 2004 - v3
Checksum:iEC9D7491B06A61E5
GO

Mass spectrometryi

Molecular mass is 12699.7 Da from positions 2 - 120. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP46571.1.
PIRiA70799.
RefSeqiNP_218261.1. NC_000962.3.
WP_003901716.1. NZ_KK339374.1.

Genome annotation databases

EnsemblBacteriaiCCP46571; CCP46571; Rv3744.
GeneIDi885418.
KEGGimtu:Rv3744.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP46571.1.
PIRiA70799.
RefSeqiNP_218261.1. NC_000962.3.
WP_003901716.1. NZ_KK339374.1.

3D structure databases

ProteinModelPortaliO69711.
SMRiO69711. Positions 2-120.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv3744.

Proteomic databases

PaxDbiO69711.
PRIDEiO69711.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP46571; CCP46571; Rv3744.
GeneIDi885418.
KEGGimtu:Rv3744.

Organism-specific databases

TubercuListiRv3744.

Phylogenomic databases

eggNOGiENOG41066UM. Bacteria.
ENOG41124TA. LUCA.
HOGENOMiHOG000144506.
InParanoidiO69711.
OMAiMIHHTRH.
OrthoDBiEOG6Z3KS4.
PhylomeDBiO69711.

Enzyme and pathway databases

BioCyciMTBRV:RV3744-MONOMER.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR001845. HTH_ArsR_DNA-bd_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01022. HTH_5. 1 hit.
[Graphical view]
PRINTSiPR00778. HTHARSR.
SMARTiSM00418. HTH_ARSR. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS50987. HTH_ARSR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  2. "A nickel-cobalt-sensing ArsR-SmtB family repressor. Contributions of cytosol and effector binding sites to metal selectivity."
    Cavet J.S., Meng W., Pennella M.A., Appelhoff R.J., Giedroc D.P., Robinson N.J.
    J. Biol. Chem. 277:38441-38448(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING, ENZYME REGULATION, GENE NAME, MUTAGENESIS OF ASP-51; ASP-91; HIS-93; HIS-104; HIS-107; HIS-109; ASP-114 AND HIS-116.
    Strain: ATCC 25618 / H37Rv.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 25618 / H37Rv.
  4. "Solution structure of Mycobacterium tuberculosis NmtR in the apo state: insights into Ni(II)-mediated allostery."
    Lee C.W., Chakravorty D.K., Chang F.M., Reyes-Caballero H., Ye Y., Merz K.M. Jr., Giedroc D.P.
    Biochemistry 51:2619-2629(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR, ENZYME REGULATION, SUBUNIT, MASS SPECTROMETRY, NICKEL-BINDING SITES.

Entry informationi

Entry nameiNMTR_MYCTU
AccessioniPrimary (citable) accession number: O69711
Secondary accession number(s): L0TGE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2012
Last sequence update: July 5, 2004
Last modified: December 9, 2015
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.