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O69600 (ALF_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fructose-bisphosphate aldolase

Short name=FBP aldolase
Short name=FBPA
EC=4.1.2.13
Alternative name(s):
Fructose-1,6-bisphosphate aldolase
Gene names
Name:fba
Ordered Locus Names:ML0286
ORF Names:MLCB4.29c
OrganismMycobacterium leprae (strain TN) [Complete proteome] [HAMAP]
Taxonomic identifier272631 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length345 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis By similarity.

Catalytic activity

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactor

Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution By similarity.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.

Sequence similarities

Belongs to the class II fructose-bisphosphate aldolase family.

Ontologies

Keywords
   Biological processGlycolysis
   LigandMetal-binding
Zinc
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglycolytic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionfructose-bisphosphate aldolase activity

Inferred from electronic annotation. Source: UniProtKB-EC

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 345345Fructose-bisphosphate aldolase
PRO_0000178721

Regions

Region253 – 2553Dihydroxyacetone phosphate binding By similarity
Region274 – 2774Dihydroxyacetone phosphate binding By similarity

Sites

Active site951Proton donor By similarity
Metal binding961Zinc 1; catalytic By similarity
Metal binding1311Zinc 2 By similarity
Metal binding1611Zinc 2 By similarity
Metal binding2121Zinc 1; catalytic By similarity
Metal binding2521Zinc 1; catalytic By similarity
Binding site531Glyceraldehyde 3-phosphate By similarity
Binding site2131Dihydroxyacetone phosphate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
O69600 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 29FE8474C41D00C7

FASTA34536,741
        10         20         30         40         50         60 
MPIATPEIYA EMLRRAKENS YAFPAINCTS SETVNAAIKG FADAGSDGII QFSTGGAEFA 

        70         80         90        100        110        120 
SGLGVKDMVT GAVALAKFTH TIAAKYPINV ALHTDHCPKD KLDSYVRPLL AISARRVATG 

       130        140        150        160        170        180 
KDPLFGSHMW DGSAIPIDEN LAIAQDLLKD AAAAKIILEV EIGVVGGEED GVAGEINEKL 

       190        200        210        220        230        240 
YTTPKDFVKT IDALGAGEHG KYLLAATFGN VHGVYKPGNV KLRPDILAEG QKVAAAKLSQ 

       250        260        270        280        290        300 
SEGSKPFDFV FHGGSGSEKS EIEEALRYGV VKMNVDTDTQ YAFTRPVSGH MFTNYDGVLK 

       310        320        330        340 
VDGDVGNKKV YDPRSYLKKA EASMTERVLE ACNDLRCAGK SVAAS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL023514 Genomic DNA. Translation: CAA18950.1.
AL583918 Genomic DNA. Translation: CAC29794.1.
PIRF86944.
RefSeqNP_301326.1. NC_002677.1.

3D structure databases

ProteinModelPortalO69600.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272631.ML0286.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC29794; CAC29794; CAC29794.
GeneID908824.
KEGGmle:ML0286.
PATRIC18051002. VBIMycLep78757_0450.

Organism-specific databases

LepromaML0286.
CMRSearch...

Phylogenomic databases

eggNOGCOG0191.
HOGENOMHOG000227794.
KOK01624.
OMAHNSLDFV.
OrthoDBEOG69GZPB.

Enzyme and pathway databases

UniPathwayUPA00109; UER00183.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR006411. Fruct_bisP_bact.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view]
PfamPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsTIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameALF_MYCLE
AccessionPrimary (citable) accession number: O69600
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: June 11, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways