Reviewed, UniProtKB/Swiss-Prot O69557 (MURE_MYCLE)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
    EC=6.3.2.13
Alternative name(s):
    UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
    Meso-diaminopimelate-adding enzyme
    Meso-A2pm-adding enzyme
    UDP-N-acetylmuramyl-tripeptide synthetase
    UDP-MurNAc-tripeptide synthetase

Gene names

Name:	murE
Ordered Locus Names:	ML0909
ORF Names:	MLCB268.07c

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	530 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP MF_00208
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00208
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity.
Sequence similarities	Belongs to the murCDEF family. MurE subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 530	530	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP MF_00208		PRO_0000101912
Regions
	Nucleotide binding	139 – 145	7	ATP Potential
	Region	181 – 182	2	UDP-MurNAc-L-Ala-D-Glu binding By similarity
	Region	434 – 437	4	Meso-diaminopimelate binding By similarity
	Motif	434 – 437	4	Meso-diaminopimelate recognition motif HAMAP MF_00208
Sites
	Binding site	52	1	UDP-MurNAc-L-Ala-D-Glu; via carbonyl oxygen By similarity
	Binding site	208	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	216	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	410	1	Meso-diaminopimelate By similarity
	Binding site	488	1	Meso-diaminopimelate; via carbonyl oxygen By similarity
	Binding site	492	1	Meso-diaminopimelate By similarity
Amino acid modifications
	Modified residue	248	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O69557-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: D042925C10E1F2FF	FASTA	530	54,808
	10 20 30 40 50 60 MTEVVAVPVR LRPSATAGVR LPELVAQVGA VLADGPGQAA TVPDIPVTGV TLRAQEVLSG 70 80 90 100 110 120 DLFAALAGAS THGARYAGVA LERGAVAVLT DVAGVAELTA QASSVPILIH PEPRSVLGGL 130 140 150 160 170 180 AAAAYGHPSN RMTVVGITGT SGKTTTTYMV EAGLRAGGRV VGLVGTIGIR IDGADIPSFL 190 200 210 220 230 240 TTPEAPALQA MLAAMVERGV ETVVMEVSSH ALSLGRVEGT QFAVAGFTNL SRDHLDFHPD 250 260 270 280 290 300 MEEYFEAKAV LFDPHSLLRA RTVVVCIDDD AGRATAARAG DAITVSALGQ PASWRATDIR 310 320 330 340 350 360 SPGVGAQEFT AVDPAGVQHR VGIRLPGRYN VANCLVALAI LDVVGVSPEQ ASLGFRDIRI 370 380 390 400 410 420 PGRLERIDCG QDFLALVDYA HKPGALHSVL TALLQPDHRL AVVFGAGGER DPGKRAPMGE 430 440 450 460 470 480 IAAELADLVV VTDDNPRGED PAAIRRDILT GTVAAGGAAQ VVEIGDRRAA IQYAVAWAGP 490 500 510 520 530 DDVVLVAGKG HETGQRGAAE TCPFDDRVEL ARALQVRDAR LLPAPGRACQ

References

[1]

"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL022602 Genomic DNA. Translation: CAA18673.1. AL583920 Genomic DNA. Translation: CAC31290.1.
PIR	G87022.
RefSeq	NP_301692.1.
3D structure databases
HSSP	HSSP built from PDB template 1E8C based on UniProtKB P22188.
ModBase	Search...
Genome annotation databases
GeneID	909910.
GenomeReviews	Gene locus ML0909 in contig AL450380_GR.
KEGG	mle:ML0909.
NMPDR	fig|272631.1.peg.564.
Organism-specific databases
Leproma	ML0909.
CMR	Search...
Phylogenomic databases
HOGENOM	O69557.
OMA	FPVIVDY.
Enzyme and pathway databases
BioCyc	MLEP272631:ML0909-MON.
BRENDA	6.3.2.13. 808.
Family and domain databases
HAMAP	MF_00208. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01085. murE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURE_MYCLE

Accession

Primary (citable) accession number: O69557

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)