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O69556 (MURF_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
EC=6.3.2.10
Alternative name(s):
D-alanyl-D-alanine-adding enzyme
UDP-MurNAc-pentapeptide synthetase
Gene names
Name:murF
Ordered Locus Names:ML0910
ORF Names:MLCB268.06c
OrganismMycobacterium leprae [Complete proteome] [HAMAP]
Taxonomic identifier1769 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length517 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein By similarity.

Catalytic activity

ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis.

Subcellular location

Cytoplasm Probable.

Sequence similarities

Belongs to the MurCDEF family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 517517UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
PRO_0000101700

Regions

Nucleotide binding138 – 1447ATP Potential

Sequences

Sequence LengthMass (Da)Tools
O69556 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: B503324330DD1FAD

FASTA51752,874
        10         20         30         40         50         60 
MIDLTVARVT EIVGGALVDI SPEEAAERRV TGSVEFDSRA VGPGGLFLAL PGARSDGHDH 

        70         80         90        100        110        120 
AASAIAAGAV AVLAARPVGV PAIVVAPDPR TGDGGLTGVL EHDADGSGAA VLAALAKLAK 

       130        140        150        160        170        180 
AVAAELVAGG LTIIGITGSS GKTSTKDLVA VVLELLGEVV APPESFNNEL GHPWTVLRAT 

       190        200        210        220        230        240 
RSTDYLILEM SARRPGNIAA LAAIAPPKIG VVLNVGTAHL GEFGSREAIA RTKTELPQAI 

       250        260        270        280        290        300 
MQSGVVILNV DDPAVAAMAD ATVARVIRVS RGSYSHPGSP NSPDVWTGPV SLDELARPRF 

       310        320        330        340        350        360 
TLHTRDPRAG ATEIQLGVYG DHQVANALCA AAVGLECGAS VEEVAVALAA TGPVSRHRMQ 

       370        380        390        400        410        420 
VTTRADGVTV IDDTYNANPD SMRAGLQALA WIAHGGTHDK NQPGSCARRS WAVLGEMAEL 

       430        440        450        460        470        480 
GEDSITEHDR IGRLAVRLDV SRLVVVGGGR SINAMHRGAV MEGSWGLETV NVADPAAALT 

       490        500        510 
LLRAEVRPGD VVLIKASHSV GLGALADVLV GDGAIRP

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AL022602 Genomic DNA. Translation: CAA18672.1.
AL583920 Genomic DNA. Translation: CAC31291.1.
PIRH87022.
RefSeqNP_301693.1. NC_002677.1.

3D structure databases

ProteinModelPortalO69556.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000029340; EBMYCP00000028946; EBMYCG00000029335.
GeneID909915.
GenomeReviewsGene locus ML0910 in contig AL450380_GR.
KEGGmle:ML0910.
NMPDRfig|272631.1.peg.565.
PATRIC18053416. VBIMycLep78757_1653.

Organism-specific databases

LepromaML0910.
CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000015779.
HOGENOMHBG617121.
OMAYAVIEMG.
ProtClustDBCLSK791663.

Enzyme and pathway databases

BioCycMLEP272631:ML0910-MONOMER.

Family and domain databases

InterProIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005863. UDP-N-AcMur-pentapeptide_synth.
[Graphical view]
Gene3DG3DSA:3.90.190.20. Mur_ligase_C. 1 hit.
G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
KOK01929.
PANTHERPTHR23135:SF3. MurF. 1 hit.
PfamPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMSSF53244. Mur_ligase_C. 1 hit.
SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMsTIGR01143. MurF. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMURF_MYCLE
AccessionPrimary (citable) accession number: O69556
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2002
Last sequence update: August 1, 1998
Last modified: January 25, 2012
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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