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Protein

UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase

Gene

murF

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein.UniRule annotation

Catalytic activityi

ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine.UniRule annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi138 – 1447ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligaseUniRule annotation (EC:6.3.2.10UniRule annotation)
Alternative name(s):
D-alanyl-D-alanine-adding enzymeUniRule annotation
UDP-MurNAc-pentapeptide synthetase
Gene namesi
Name:murFUniRule annotation
Ordered Locus Names:ML0910
ORF Names:MLCB268.06c
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML0910.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 517517UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligasePRO_0000101700Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272631.ML0910.

Structurei

3D structure databases

ProteinModelPortaliO69556.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MurCDEF family. MurF subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107EES. Bacteria.
COG0770. LUCA.
HOGENOMiHOG000268120.
KOiK01929.
OMAiDYAVIEM.
OrthoDBiPOG091H00FE.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.1390.10. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_02019. MurF. 1 hit.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005863. UDP-N-AcMur_synth.
[Graphical view]
PANTHERiPTHR23135:SF3. PTHR23135:SF3. 1 hit.
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
SSF63418. SSF63418. 1 hit.
TIGRFAMsiTIGR01143. murF. 1 hit.

Sequencei

Sequence statusi: Complete.

O69556-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIDLTVARVT EIVGGALVDI SPEEAAERRV TGSVEFDSRA VGPGGLFLAL
60 70 80 90 100
PGARSDGHDH AASAIAAGAV AVLAARPVGV PAIVVAPDPR TGDGGLTGVL
110 120 130 140 150
EHDADGSGAA VLAALAKLAK AVAAELVAGG LTIIGITGSS GKTSTKDLVA
160 170 180 190 200
VVLELLGEVV APPESFNNEL GHPWTVLRAT RSTDYLILEM SARRPGNIAA
210 220 230 240 250
LAAIAPPKIG VVLNVGTAHL GEFGSREAIA RTKTELPQAI MQSGVVILNV
260 270 280 290 300
DDPAVAAMAD ATVARVIRVS RGSYSHPGSP NSPDVWTGPV SLDELARPRF
310 320 330 340 350
TLHTRDPRAG ATEIQLGVYG DHQVANALCA AAVGLECGAS VEEVAVALAA
360 370 380 390 400
TGPVSRHRMQ VTTRADGVTV IDDTYNANPD SMRAGLQALA WIAHGGTHDK
410 420 430 440 450
NQPGSCARRS WAVLGEMAEL GEDSITEHDR IGRLAVRLDV SRLVVVGGGR
460 470 480 490 500
SINAMHRGAV MEGSWGLETV NVADPAAALT LLRAEVRPGD VVLIKASHSV
510
GLGALADVLV GDGAIRP
Length:517
Mass (Da):52,874
Last modified:August 1, 1998 - v1
Checksum:iB503324330DD1FAD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL022602 Genomic DNA. Translation: CAA18672.1.
AL583920 Genomic DNA. Translation: CAC31291.1.
PIRiH87022.
RefSeqiNP_301693.1. NC_002677.1.
WP_010908017.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC31291; CAC31291; CAC31291.
GeneIDi909915.
KEGGimle:ML0910.
PATRICi18053416. VBIMycLep78757_1653.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL022602 Genomic DNA. Translation: CAA18672.1.
AL583920 Genomic DNA. Translation: CAC31291.1.
PIRiH87022.
RefSeqiNP_301693.1. NC_002677.1.
WP_010908017.1. NC_002677.1.

3D structure databases

ProteinModelPortaliO69556.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML0910.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC31291; CAC31291; CAC31291.
GeneIDi909915.
KEGGimle:ML0910.
PATRICi18053416. VBIMycLep78757_1653.

Organism-specific databases

LepromaiML0910.

Phylogenomic databases

eggNOGiENOG4107EES. Bacteria.
COG0770. LUCA.
HOGENOMiHOG000268120.
KOiK01929.
OMAiDYAVIEM.
OrthoDBiPOG091H00FE.

Enzyme and pathway databases

UniPathwayiUPA00219.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.1390.10. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_02019. MurF. 1 hit.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005863. UDP-N-AcMur_synth.
[Graphical view]
PANTHERiPTHR23135:SF3. PTHR23135:SF3. 1 hit.
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
SSF63418. SSF63418. 1 hit.
TIGRFAMsiTIGR01143. murF. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMURF_MYCLE
AccessioniPrimary (citable) accession number: O69556
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2002
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.