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O69548

- TOP1_MYCLE

UniProt

O69548 - TOP1_MYCLE

Protein

DNA topoisomerase 1

Gene

topA

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

    Cofactori

    Magnesium. Binds two Mg2+ per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi22 – 221Magnesium 1; catalyticUniRule annotation
    Sitei46 – 461Interaction with DNAUniRule annotation
    Metal bindingi109 – 1091Magnesium 1; catalyticUniRule annotation
    Metal bindingi109 – 1091Magnesium 2UniRule annotation
    Metal bindingi111 – 1111Magnesium 2UniRule annotation
    Sitei165 – 1651Interaction with DNAUniRule annotation
    Sitei166 – 1661Interaction with DNAUniRule annotation
    Sitei169 – 1691Interaction with DNAUniRule annotation
    Sitei174 – 1741Interaction with DNAUniRule annotation
    Sitei181 – 1811Interaction with DNAUniRule annotation
    Active sitei343 – 3431O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
    Sitei345 – 3451Interaction with DNAUniRule annotation
    Sitei545 – 5451Interaction with DNAUniRule annotation

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA topological change Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
    Alternative name(s):
    DNA topoisomerase IUniRule annotation
    Omega-protein
    Relaxing enzyme
    Swivelase
    Untwisting enzyme
    Gene namesi
    Name:topAUniRule annotation
    Ordered Locus Names:ML0200
    ORF Names:MLCB2548.31c
    OrganismiMycobacterium leprae (strain TN)
    Taxonomic identifieri272631 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000806: Chromosome

    Organism-specific databases

    LepromaiML0200.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 947947DNA topoisomerase 1PRO_0000145158Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi272631.ML0200.

    Structurei

    3D structure databases

    ProteinModelPortaliO69548.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini16 – 140125ToprimUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni189 – 1946Interaction with DNAUniRule annotation

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.UniRule annotation
    Contains 1 Toprim domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1754.
    HOGENOMiHOG000004020.
    KOiK03168.
    OMAiANEGYER.
    OrthoDBiEOG6S7XQ9.

    Family and domain databases

    Gene3Di1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPiMF_00952. Topoisom_1_prok.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR005733. TopoI_bac-type.
    IPR028612. Topoisom_1_IA.
    IPR025589. Toprim_C_rpt.
    IPR006171. Toprim_domain.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF13368. Toprim_C_rpt. 3 hits.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 1 hit.
    TIGRFAMsiTIGR01051. topA_bact. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O69548-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKGPRGSRSG RNGSPRRLVI VESPTKARKL SGYLGSAYIV ESSRGHIRDL    50
    PRTAADVPAK FKSEPWARLG VNVDADFEPL YIITPEKKST VTELKGLLQG 100
    VDELYLATDG DREGEAIAWH LMQTLKPRVP VKRMVFHEIT EHAILAAAAH 150
    PRELDIDLVD AQETRRILDR LYGYEVSPVL WKKVAPKLSA GRVQSVATRI 200
    IVQRERDRMA FCSAVYWDIV AKLDASVSDP TAQPLTFVAR LTAVDGLRVA 250
    TGRDFGAMGT LRGDCEVSKI IVLDDLTATT LAAGLRGAQL TVASAEEKLY 300
    TRRPYPPFMT STLQQEAGRK LRFSAERTMS IAQRLYENGY ITYMRTDSTT 350
    LSESAINAAR TQARQLYGEE YVSDSPRQYT RKVKNAQEAH EAIRPAGETF 400
    ATPDAVCNEL DGDEFRIYEL VWRRTVASQM ADARGTTLSL RIEGRAGEQH 450
    VVFSASGRTL TFPGFLKAYV ETVDELAGGE ADDAERRLPH LTSGQLLDVI 500
    DLTPDGHATN PPARYTEASL VKALEELGIG RPSTYSSIIK TVQDRGYVQK 550
    KGSALVPSWV AFAVTGLLEQ HFGRLVDYDF TAAMEDELDE IANGNEQRTN 600
    WLNNFYFGGN HGVSDSIARS GGLKKLVGVN IEGIDAREVN SIKLFDDEHG 650
    RPVYVRVGKT GPYLERLLAG DDGELTPQRA NLNGTLTPDE LTLEVAEELF 700
    ATPHEGRVLG VDPETGHEIV AKDGRYGPYV TEVLPKHDDD YGAADQGTKK 750
    TKKGRRASAS QGPKPRTGSL LRSMDLQTIT LEDALKLLLL PRVVGVDPAS 800
    GEEITAQNGR YGPYLKRGKD SRPLTTEDQM FIITLDEALK IYAEPKRAGR 850
    QSTSPPPLRE LGTDPTSGKP MLIKDGRFGP YVTDGETNAS LRKGDDVVSI 900
    TDERAAELLA DRRARGPVKR PAKKARKVPA KKAARLAPAR GISQSPR 947
    Length:947
    Mass (Da):104,056
    Last modified:August 1, 1998 - v1
    Checksum:i4ACBA00C783D23AF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL023093 Genomic DNA. Translation: CAA18818.1.
    AL583917 Genomic DNA. Translation: CAC29708.1.
    PIRiH86933.
    RefSeqiNP_301268.1. NC_002677.1.
    WP_010907592.1. NC_002677.1.

    Genome annotation databases

    EnsemblBacteriaiCAC29708; CAC29708; CAC29708.
    GeneIDi908599.
    KEGGimle:ML0200.
    PATRICi18050732. VBIMycLep78757_0318.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL023093 Genomic DNA. Translation: CAA18818.1 .
    AL583917 Genomic DNA. Translation: CAC29708.1 .
    PIRi H86933.
    RefSeqi NP_301268.1. NC_002677.1.
    WP_010907592.1. NC_002677.1.

    3D structure databases

    ProteinModelPortali O69548.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272631.ML0200.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAC29708 ; CAC29708 ; CAC29708 .
    GeneIDi 908599.
    KEGGi mle:ML0200.
    PATRICi 18050732. VBIMycLep78757_0318.

    Organism-specific databases

    Lepromai ML0200.

    Phylogenomic databases

    eggNOGi COG1754.
    HOGENOMi HOG000004020.
    KOi K03168.
    OMAi ANEGYER.
    OrthoDBi EOG6S7XQ9.

    Family and domain databases

    Gene3Di 1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPi MF_00952. Topoisom_1_prok.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR005733. TopoI_bac-type.
    IPR028612. Topoisom_1_IA.
    IPR025589. Toprim_C_rpt.
    IPR006171. Toprim_domain.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF13368. Toprim_C_rpt. 3 hits.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 1 hit.
    TIGRFAMsi TIGR01051. topA_bact. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: TN.

    Entry informationi

    Entry nameiTOP1_MYCLE
    AccessioniPrimary (citable) accession number: O69548
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3