Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA topoisomerase 1

Gene

topA

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi22Magnesium 1; catalyticUniRule annotation1
Metal bindingi109Magnesium 1; catalyticUniRule annotation1
Metal bindingi109Magnesium 2UniRule annotation1
Metal bindingi111Magnesium 2UniRule annotation1
Active sitei343O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciMLEP272631:G1GT5-226-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topAUniRule annotation
Ordered Locus Names:ML0200
ORF Names:MLCB2548.31c
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML0200

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001451581 – 947DNA topoisomerase 1Add BLAST947

Proteomic databases

PRIDEiO69548

Interactioni

Subunit structurei

Monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei46Interaction with DNAUniRule annotation1
Sitei165Interaction with DNAUniRule annotation1
Sitei166Interaction with DNAUniRule annotation1
Sitei169Interaction with DNAUniRule annotation1
Sitei174Interaction with DNAUniRule annotation1
Sitei181Interaction with DNAUniRule annotation1
Sitei345Interaction with DNAUniRule annotation1
Sitei545Interaction with DNAUniRule annotation1

Protein-protein interaction databases

STRINGi272631.ML0200

Structurei

3D structure databases

ProteinModelPortaliO69548
SMRiO69548
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 140ToprimUniRule annotationAdd BLAST125

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni189 – 194Interaction with DNAUniRule annotation6

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C73 Bacteria
COG0550 LUCA
COG1754 LUCA
HOGENOMiHOG000004020
KOiK03168
OMAiIDFPGFF
OrthoDBiPOG091H02BN

Family and domain databases

CDDicd00186 TOP1Ac, 1 hit
cd03363 TOPRIM_TopoIA_TopoI, 1 hit
Gene3Di1.10.290.10, 1 hit
1.10.460.10, 2 hits
2.70.20.10, 2 hits
HAMAPiMF_00952 Topoisom_1_prok, 1 hit
InterProiView protein in InterPro
IPR000380 Topo_IA
IPR003601 Topo_IA_2
IPR023406 Topo_IA_AS
IPR013497 Topo_IA_cen
IPR013824 Topo_IA_cen_sub1
IPR013825 Topo_IA_cen_sub2
IPR013826 Topo_IA_cen_sub3
IPR023405 Topo_IA_core_domain
IPR003602 Topo_IA_DNA-bd_dom
IPR005733 TopoI_bac-type
IPR028612 Topoisom_1_IA
IPR025589 Toprim_C_rpt
IPR006171 TOPRIM_domain
IPR034149 TOPRIM_TopoI
PANTHERiPTHR42785 PTHR42785, 1 hit
PfamiView protein in Pfam
PF01131 Topoisom_bac, 1 hit
PF01751 Toprim, 1 hit
PF13368 Toprim_C_rpt, 4 hits
PRINTSiPR00417 PRTPISMRASEI
SMARTiView protein in SMART
SM00437 TOP1Ac, 1 hit
SM00436 TOP1Bc, 1 hit
SM00493 TOPRIM, 1 hit
SUPFAMiSSF56712 SSF56712, 1 hit
TIGRFAMsiTIGR01051 topA_bact, 1 hit
PROSITEiView protein in PROSITE
PS00396 TOPOISOMERASE_I_PROK, 1 hit
PS50880 TOPRIM, 1 hit

Sequencei

Sequence statusi: Complete.

O69548-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGPRGSRSG RNGSPRRLVI VESPTKARKL SGYLGSAYIV ESSRGHIRDL
60 70 80 90 100
PRTAADVPAK FKSEPWARLG VNVDADFEPL YIITPEKKST VTELKGLLQG
110 120 130 140 150
VDELYLATDG DREGEAIAWH LMQTLKPRVP VKRMVFHEIT EHAILAAAAH
160 170 180 190 200
PRELDIDLVD AQETRRILDR LYGYEVSPVL WKKVAPKLSA GRVQSVATRI
210 220 230 240 250
IVQRERDRMA FCSAVYWDIV AKLDASVSDP TAQPLTFVAR LTAVDGLRVA
260 270 280 290 300
TGRDFGAMGT LRGDCEVSKI IVLDDLTATT LAAGLRGAQL TVASAEEKLY
310 320 330 340 350
TRRPYPPFMT STLQQEAGRK LRFSAERTMS IAQRLYENGY ITYMRTDSTT
360 370 380 390 400
LSESAINAAR TQARQLYGEE YVSDSPRQYT RKVKNAQEAH EAIRPAGETF
410 420 430 440 450
ATPDAVCNEL DGDEFRIYEL VWRRTVASQM ADARGTTLSL RIEGRAGEQH
460 470 480 490 500
VVFSASGRTL TFPGFLKAYV ETVDELAGGE ADDAERRLPH LTSGQLLDVI
510 520 530 540 550
DLTPDGHATN PPARYTEASL VKALEELGIG RPSTYSSIIK TVQDRGYVQK
560 570 580 590 600
KGSALVPSWV AFAVTGLLEQ HFGRLVDYDF TAAMEDELDE IANGNEQRTN
610 620 630 640 650
WLNNFYFGGN HGVSDSIARS GGLKKLVGVN IEGIDAREVN SIKLFDDEHG
660 670 680 690 700
RPVYVRVGKT GPYLERLLAG DDGELTPQRA NLNGTLTPDE LTLEVAEELF
710 720 730 740 750
ATPHEGRVLG VDPETGHEIV AKDGRYGPYV TEVLPKHDDD YGAADQGTKK
760 770 780 790 800
TKKGRRASAS QGPKPRTGSL LRSMDLQTIT LEDALKLLLL PRVVGVDPAS
810 820 830 840 850
GEEITAQNGR YGPYLKRGKD SRPLTTEDQM FIITLDEALK IYAEPKRAGR
860 870 880 890 900
QSTSPPPLRE LGTDPTSGKP MLIKDGRFGP YVTDGETNAS LRKGDDVVSI
910 920 930 940
TDERAAELLA DRRARGPVKR PAKKARKVPA KKAARLAPAR GISQSPR
Length:947
Mass (Da):104,056
Last modified:August 1, 1998 - v1
Checksum:i4ACBA00C783D23AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL023093 Genomic DNA Translation: CAA18818.1
AL583917 Genomic DNA Translation: CAC29708.1
PIRiH86933
RefSeqiNP_301268.1, NC_002677.1
WP_010907592.1, NC_002677.1

Genome annotation databases

EnsemblBacteriaiCAC29708; CAC29708; CAC29708
GeneIDi908599
KEGGimle:ML0200
PATRICifig|272631.5.peg.318

Similar proteinsi

Entry informationi

Entry nameiTOP1_MYCLE
AccessioniPrimary (citable) accession number: O69548
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: May 23, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health