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Protein

DNA topoisomerase 1

Gene

topA

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi22Magnesium 1; catalyticUniRule annotation1
Metal bindingi109Magnesium 1; catalyticUniRule annotation1
Metal bindingi109Magnesium 2UniRule annotation1
Metal bindingi111Magnesium 2UniRule annotation1
Active sitei343O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topAUniRule annotation
Ordered Locus Names:ML0200
ORF Names:MLCB2548.31c
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML0200.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001451581 – 947DNA topoisomerase 1Add BLAST947

Interactioni

Subunit structurei

Monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei46Interaction with DNAUniRule annotation1
Sitei165Interaction with DNAUniRule annotation1
Sitei166Interaction with DNAUniRule annotation1
Sitei169Interaction with DNAUniRule annotation1
Sitei174Interaction with DNAUniRule annotation1
Sitei181Interaction with DNAUniRule annotation1
Sitei345Interaction with DNAUniRule annotation1
Sitei545Interaction with DNAUniRule annotation1

Protein-protein interaction databases

STRINGi272631.ML0200.

Structurei

3D structure databases

ProteinModelPortaliO69548.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 140ToprimUniRule annotationAdd BLAST125

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni189 – 194Interaction with DNAUniRule annotation6

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C73. Bacteria.
COG0550. LUCA.
COG1754. LUCA.
HOGENOMiHOG000004020.
KOiK03168.
OMAiVEACIGH.
OrthoDBiPOG091H02BN.

Family and domain databases

CDDicd00186. TOP1Ac. 1 hit.
Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR025589. Toprim_C_rpt.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 2 hits.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF13368. Toprim_C_rpt. 4 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O69548-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGPRGSRSG RNGSPRRLVI VESPTKARKL SGYLGSAYIV ESSRGHIRDL
60 70 80 90 100
PRTAADVPAK FKSEPWARLG VNVDADFEPL YIITPEKKST VTELKGLLQG
110 120 130 140 150
VDELYLATDG DREGEAIAWH LMQTLKPRVP VKRMVFHEIT EHAILAAAAH
160 170 180 190 200
PRELDIDLVD AQETRRILDR LYGYEVSPVL WKKVAPKLSA GRVQSVATRI
210 220 230 240 250
IVQRERDRMA FCSAVYWDIV AKLDASVSDP TAQPLTFVAR LTAVDGLRVA
260 270 280 290 300
TGRDFGAMGT LRGDCEVSKI IVLDDLTATT LAAGLRGAQL TVASAEEKLY
310 320 330 340 350
TRRPYPPFMT STLQQEAGRK LRFSAERTMS IAQRLYENGY ITYMRTDSTT
360 370 380 390 400
LSESAINAAR TQARQLYGEE YVSDSPRQYT RKVKNAQEAH EAIRPAGETF
410 420 430 440 450
ATPDAVCNEL DGDEFRIYEL VWRRTVASQM ADARGTTLSL RIEGRAGEQH
460 470 480 490 500
VVFSASGRTL TFPGFLKAYV ETVDELAGGE ADDAERRLPH LTSGQLLDVI
510 520 530 540 550
DLTPDGHATN PPARYTEASL VKALEELGIG RPSTYSSIIK TVQDRGYVQK
560 570 580 590 600
KGSALVPSWV AFAVTGLLEQ HFGRLVDYDF TAAMEDELDE IANGNEQRTN
610 620 630 640 650
WLNNFYFGGN HGVSDSIARS GGLKKLVGVN IEGIDAREVN SIKLFDDEHG
660 670 680 690 700
RPVYVRVGKT GPYLERLLAG DDGELTPQRA NLNGTLTPDE LTLEVAEELF
710 720 730 740 750
ATPHEGRVLG VDPETGHEIV AKDGRYGPYV TEVLPKHDDD YGAADQGTKK
760 770 780 790 800
TKKGRRASAS QGPKPRTGSL LRSMDLQTIT LEDALKLLLL PRVVGVDPAS
810 820 830 840 850
GEEITAQNGR YGPYLKRGKD SRPLTTEDQM FIITLDEALK IYAEPKRAGR
860 870 880 890 900
QSTSPPPLRE LGTDPTSGKP MLIKDGRFGP YVTDGETNAS LRKGDDVVSI
910 920 930 940
TDERAAELLA DRRARGPVKR PAKKARKVPA KKAARLAPAR GISQSPR
Length:947
Mass (Da):104,056
Last modified:August 1, 1998 - v1
Checksum:i4ACBA00C783D23AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL023093 Genomic DNA. Translation: CAA18818.1.
AL583917 Genomic DNA. Translation: CAC29708.1.
PIRiH86933.
RefSeqiNP_301268.1. NC_002677.1.
WP_010907592.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC29708; CAC29708; CAC29708.
GeneIDi908599.
KEGGimle:ML0200.
PATRICi18050732. VBIMycLep78757_0318.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL023093 Genomic DNA. Translation: CAA18818.1.
AL583917 Genomic DNA. Translation: CAC29708.1.
PIRiH86933.
RefSeqiNP_301268.1. NC_002677.1.
WP_010907592.1. NC_002677.1.

3D structure databases

ProteinModelPortaliO69548.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML0200.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC29708; CAC29708; CAC29708.
GeneIDi908599.
KEGGimle:ML0200.
PATRICi18050732. VBIMycLep78757_0318.

Organism-specific databases

LepromaiML0200.

Phylogenomic databases

eggNOGiENOG4105C73. Bacteria.
COG0550. LUCA.
COG1754. LUCA.
HOGENOMiHOG000004020.
KOiK03168.
OMAiVEACIGH.
OrthoDBiPOG091H02BN.

Family and domain databases

CDDicd00186. TOP1Ac. 1 hit.
Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR025589. Toprim_C_rpt.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 2 hits.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF13368. Toprim_C_rpt. 4 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTOP1_MYCLE
AccessioniPrimary (citable) accession number: O69548
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.