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O69548 (TOP1_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene names
Name:topA
Ordered Locus Names:ML0200
ORF Names:MLCB2548.31c
OrganismMycobacterium leprae (strain TN) [Complete proteome] [HAMAP]
Taxonomic identifier272631 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length947 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00952

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00952

Cofactor

Magnesium. Binds two Mg2+ per subunit By similarity. HAMAP-Rule MF_00952

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00952

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 947947DNA topoisomerase 1 HAMAP-Rule MF_00952
PRO_0000145158

Regions

Domain16 – 140125Toprim
Region189 – 1946Interaction with DNA By similarity

Sites

Active site3431O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding221Magnesium 1; catalytic By similarity
Metal binding1091Magnesium 1; catalytic By similarity
Metal binding1091Magnesium 2 By similarity
Metal binding1111Magnesium 2 By similarity
Site461Interaction with DNA By similarity
Site1651Interaction with DNA By similarity
Site1661Interaction with DNA By similarity
Site1691Interaction with DNA By similarity
Site1741Interaction with DNA By similarity
Site1811Interaction with DNA By similarity
Site3451Interaction with DNA By similarity
Site5451Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
O69548 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 4ACBA00C783D23AF

FASTA947104,056
        10         20         30         40         50         60 
MKGPRGSRSG RNGSPRRLVI VESPTKARKL SGYLGSAYIV ESSRGHIRDL PRTAADVPAK 

        70         80         90        100        110        120 
FKSEPWARLG VNVDADFEPL YIITPEKKST VTELKGLLQG VDELYLATDG DREGEAIAWH 

       130        140        150        160        170        180 
LMQTLKPRVP VKRMVFHEIT EHAILAAAAH PRELDIDLVD AQETRRILDR LYGYEVSPVL 

       190        200        210        220        230        240 
WKKVAPKLSA GRVQSVATRI IVQRERDRMA FCSAVYWDIV AKLDASVSDP TAQPLTFVAR 

       250        260        270        280        290        300 
LTAVDGLRVA TGRDFGAMGT LRGDCEVSKI IVLDDLTATT LAAGLRGAQL TVASAEEKLY 

       310        320        330        340        350        360 
TRRPYPPFMT STLQQEAGRK LRFSAERTMS IAQRLYENGY ITYMRTDSTT LSESAINAAR 

       370        380        390        400        410        420 
TQARQLYGEE YVSDSPRQYT RKVKNAQEAH EAIRPAGETF ATPDAVCNEL DGDEFRIYEL 

       430        440        450        460        470        480 
VWRRTVASQM ADARGTTLSL RIEGRAGEQH VVFSASGRTL TFPGFLKAYV ETVDELAGGE 

       490        500        510        520        530        540 
ADDAERRLPH LTSGQLLDVI DLTPDGHATN PPARYTEASL VKALEELGIG RPSTYSSIIK 

       550        560        570        580        590        600 
TVQDRGYVQK KGSALVPSWV AFAVTGLLEQ HFGRLVDYDF TAAMEDELDE IANGNEQRTN 

       610        620        630        640        650        660 
WLNNFYFGGN HGVSDSIARS GGLKKLVGVN IEGIDAREVN SIKLFDDEHG RPVYVRVGKT 

       670        680        690        700        710        720 
GPYLERLLAG DDGELTPQRA NLNGTLTPDE LTLEVAEELF ATPHEGRVLG VDPETGHEIV 

       730        740        750        760        770        780 
AKDGRYGPYV TEVLPKHDDD YGAADQGTKK TKKGRRASAS QGPKPRTGSL LRSMDLQTIT 

       790        800        810        820        830        840 
LEDALKLLLL PRVVGVDPAS GEEITAQNGR YGPYLKRGKD SRPLTTEDQM FIITLDEALK 

       850        860        870        880        890        900 
IYAEPKRAGR QSTSPPPLRE LGTDPTSGKP MLIKDGRFGP YVTDGETNAS LRKGDDVVSI 

       910        920        930        940 
TDERAAELLA DRRARGPVKR PAKKARKVPA KKAARLAPAR GISQSPR 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL023093 Genomic DNA. Translation: CAA18818.1.
AL583917 Genomic DNA. Translation: CAC29708.1.
PIRH86933.
RefSeqNP_301268.1. NC_002677.1.

3D structure databases

ProteinModelPortalO69548.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272631.ML0200.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC29708; CAC29708; CAC29708.
GeneID908599.
KEGGmle:ML0200.
PATRIC18050732. VBIMycLep78757_0318.

Organism-specific databases

LepromaML0200.
CMRSearch...

Phylogenomic databases

eggNOGCOG1754.
HOGENOMHOG000004020.
KOK03168.
OMATIMDRGY.
OrthoDBEOG6S7XQ9.
ProtClustDBPRK07561.

Family and domain databases

Gene3D1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPMF_00952. Topoisom_1_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR025589. Toprim_C_rpt.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF13368. Toprim_C_rpt. 3 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 1 hit.
TIGRFAMsTIGR01051. topA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_MYCLE
AccessionPrimary (citable) accession number: O69548
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: February 19, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families