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O69548

- TOP1_MYCLE

UniProt

O69548 - TOP1_MYCLE

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Protein

DNA topoisomerase 1

Gene
topA, ML0200, MLCB2548.31c
Organism
Mycobacterium leprae (strain TN)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi22 – 221Magnesium 1; catalytic By similarity
Sitei46 – 461Interaction with DNA By similarity
Metal bindingi109 – 1091Magnesium 1; catalytic By similarity
Metal bindingi109 – 1091Magnesium 2 By similarity
Metal bindingi111 – 1111Magnesium 2 By similarity
Sitei165 – 1651Interaction with DNA By similarity
Sitei166 – 1661Interaction with DNA By similarity
Sitei169 – 1691Interaction with DNA By similarity
Sitei174 – 1741Interaction with DNA By similarity
Sitei181 – 1811Interaction with DNA By similarity
Active sitei343 – 3431O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Sitei345 – 3451Interaction with DNA By similarity
Sitei545 – 5451Interaction with DNA By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topA
Ordered Locus Names:ML0200
ORF Names:MLCB2548.31c
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000806: Chromosome

Organism-specific databases

LepromaiML0200.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 947947DNA topoisomerase 1UniRule annotationPRO_0000145158Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi272631.ML0200.

Structurei

3D structure databases

ProteinModelPortaliO69548.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 140125ToprimAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni189 – 1946Interaction with DNA By similarity

Sequence similaritiesi

Contains 1 Toprim domain.

Phylogenomic databases

eggNOGiCOG1754.
HOGENOMiHOG000004020.
KOiK03168.
OMAiANEGYER.
OrthoDBiEOG6S7XQ9.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR025589. Toprim_C_rpt.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF13368. Toprim_C_rpt. 3 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O69548-1 [UniParc]FASTAAdd to Basket

« Hide

MKGPRGSRSG RNGSPRRLVI VESPTKARKL SGYLGSAYIV ESSRGHIRDL    50
PRTAADVPAK FKSEPWARLG VNVDADFEPL YIITPEKKST VTELKGLLQG 100
VDELYLATDG DREGEAIAWH LMQTLKPRVP VKRMVFHEIT EHAILAAAAH 150
PRELDIDLVD AQETRRILDR LYGYEVSPVL WKKVAPKLSA GRVQSVATRI 200
IVQRERDRMA FCSAVYWDIV AKLDASVSDP TAQPLTFVAR LTAVDGLRVA 250
TGRDFGAMGT LRGDCEVSKI IVLDDLTATT LAAGLRGAQL TVASAEEKLY 300
TRRPYPPFMT STLQQEAGRK LRFSAERTMS IAQRLYENGY ITYMRTDSTT 350
LSESAINAAR TQARQLYGEE YVSDSPRQYT RKVKNAQEAH EAIRPAGETF 400
ATPDAVCNEL DGDEFRIYEL VWRRTVASQM ADARGTTLSL RIEGRAGEQH 450
VVFSASGRTL TFPGFLKAYV ETVDELAGGE ADDAERRLPH LTSGQLLDVI 500
DLTPDGHATN PPARYTEASL VKALEELGIG RPSTYSSIIK TVQDRGYVQK 550
KGSALVPSWV AFAVTGLLEQ HFGRLVDYDF TAAMEDELDE IANGNEQRTN 600
WLNNFYFGGN HGVSDSIARS GGLKKLVGVN IEGIDAREVN SIKLFDDEHG 650
RPVYVRVGKT GPYLERLLAG DDGELTPQRA NLNGTLTPDE LTLEVAEELF 700
ATPHEGRVLG VDPETGHEIV AKDGRYGPYV TEVLPKHDDD YGAADQGTKK 750
TKKGRRASAS QGPKPRTGSL LRSMDLQTIT LEDALKLLLL PRVVGVDPAS 800
GEEITAQNGR YGPYLKRGKD SRPLTTEDQM FIITLDEALK IYAEPKRAGR 850
QSTSPPPLRE LGTDPTSGKP MLIKDGRFGP YVTDGETNAS LRKGDDVVSI 900
TDERAAELLA DRRARGPVKR PAKKARKVPA KKAARLAPAR GISQSPR 947
Length:947
Mass (Da):104,056
Last modified:August 1, 1998 - v1
Checksum:i4ACBA00C783D23AF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL023093 Genomic DNA. Translation: CAA18818.1.
AL583917 Genomic DNA. Translation: CAC29708.1.
PIRiH86933.
RefSeqiNP_301268.1. NC_002677.1.
WP_010907592.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC29708; CAC29708; CAC29708.
GeneIDi908599.
KEGGimle:ML0200.
PATRICi18050732. VBIMycLep78757_0318.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL023093 Genomic DNA. Translation: CAA18818.1 .
AL583917 Genomic DNA. Translation: CAC29708.1 .
PIRi H86933.
RefSeqi NP_301268.1. NC_002677.1.
WP_010907592.1. NC_002677.1.

3D structure databases

ProteinModelPortali O69548.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272631.ML0200.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAC29708 ; CAC29708 ; CAC29708 .
GeneIDi 908599.
KEGGi mle:ML0200.
PATRICi 18050732. VBIMycLep78757_0318.

Organism-specific databases

Lepromai ML0200.

Phylogenomic databases

eggNOGi COG1754.
HOGENOMi HOG000004020.
KOi K03168.
OMAi ANEGYER.
OrthoDBi EOG6S7XQ9.

Family and domain databases

Gene3Di 1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR025589. Toprim_C_rpt.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF13368. Toprim_C_rpt. 3 hits.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TN.

Entry informationi

Entry nameiTOP1_MYCLE
AccessioniPrimary (citable) accession number: O69548
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: September 3, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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