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O69548

- TOP1_MYCLE

UniProt

O69548 - TOP1_MYCLE

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Protein

DNA topoisomerase 1

Gene

topA

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi22 – 221Magnesium 1; catalyticUniRule annotation
Sitei46 – 461Interaction with DNAUniRule annotation
Metal bindingi109 – 1091Magnesium 1; catalyticUniRule annotation
Metal bindingi109 – 1091Magnesium 2UniRule annotation
Metal bindingi111 – 1111Magnesium 2UniRule annotation
Sitei165 – 1651Interaction with DNAUniRule annotation
Sitei166 – 1661Interaction with DNAUniRule annotation
Sitei169 – 1691Interaction with DNAUniRule annotation
Sitei174 – 1741Interaction with DNAUniRule annotation
Sitei181 – 1811Interaction with DNAUniRule annotation
Active sitei343 – 3431O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
Sitei345 – 3451Interaction with DNAUniRule annotation
Sitei545 – 5451Interaction with DNAUniRule annotation

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topAUniRule annotation
Ordered Locus Names:ML0200
ORF Names:MLCB2548.31c
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000806: Chromosome

Organism-specific databases

LepromaiML0200.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 947947DNA topoisomerase 1PRO_0000145158Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272631.ML0200.

Structurei

3D structure databases

ProteinModelPortaliO69548.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 140125ToprimUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni189 – 1946Interaction with DNAUniRule annotation

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1754.
HOGENOMiHOG000004020.
KOiK03168.
OMAiANEGYER.
OrthoDBiEOG6S7XQ9.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR025589. Toprim_C_rpt.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF13368. Toprim_C_rpt. 3 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O69548-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKGPRGSRSG RNGSPRRLVI VESPTKARKL SGYLGSAYIV ESSRGHIRDL
60 70 80 90 100
PRTAADVPAK FKSEPWARLG VNVDADFEPL YIITPEKKST VTELKGLLQG
110 120 130 140 150
VDELYLATDG DREGEAIAWH LMQTLKPRVP VKRMVFHEIT EHAILAAAAH
160 170 180 190 200
PRELDIDLVD AQETRRILDR LYGYEVSPVL WKKVAPKLSA GRVQSVATRI
210 220 230 240 250
IVQRERDRMA FCSAVYWDIV AKLDASVSDP TAQPLTFVAR LTAVDGLRVA
260 270 280 290 300
TGRDFGAMGT LRGDCEVSKI IVLDDLTATT LAAGLRGAQL TVASAEEKLY
310 320 330 340 350
TRRPYPPFMT STLQQEAGRK LRFSAERTMS IAQRLYENGY ITYMRTDSTT
360 370 380 390 400
LSESAINAAR TQARQLYGEE YVSDSPRQYT RKVKNAQEAH EAIRPAGETF
410 420 430 440 450
ATPDAVCNEL DGDEFRIYEL VWRRTVASQM ADARGTTLSL RIEGRAGEQH
460 470 480 490 500
VVFSASGRTL TFPGFLKAYV ETVDELAGGE ADDAERRLPH LTSGQLLDVI
510 520 530 540 550
DLTPDGHATN PPARYTEASL VKALEELGIG RPSTYSSIIK TVQDRGYVQK
560 570 580 590 600
KGSALVPSWV AFAVTGLLEQ HFGRLVDYDF TAAMEDELDE IANGNEQRTN
610 620 630 640 650
WLNNFYFGGN HGVSDSIARS GGLKKLVGVN IEGIDAREVN SIKLFDDEHG
660 670 680 690 700
RPVYVRVGKT GPYLERLLAG DDGELTPQRA NLNGTLTPDE LTLEVAEELF
710 720 730 740 750
ATPHEGRVLG VDPETGHEIV AKDGRYGPYV TEVLPKHDDD YGAADQGTKK
760 770 780 790 800
TKKGRRASAS QGPKPRTGSL LRSMDLQTIT LEDALKLLLL PRVVGVDPAS
810 820 830 840 850
GEEITAQNGR YGPYLKRGKD SRPLTTEDQM FIITLDEALK IYAEPKRAGR
860 870 880 890 900
QSTSPPPLRE LGTDPTSGKP MLIKDGRFGP YVTDGETNAS LRKGDDVVSI
910 920 930 940
TDERAAELLA DRRARGPVKR PAKKARKVPA KKAARLAPAR GISQSPR
Length:947
Mass (Da):104,056
Last modified:August 1, 1998 - v1
Checksum:i4ACBA00C783D23AF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL023093 Genomic DNA. Translation: CAA18818.1.
AL583917 Genomic DNA. Translation: CAC29708.1.
PIRiH86933.
RefSeqiNP_301268.1. NC_002677.1.
WP_010907592.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC29708; CAC29708; CAC29708.
GeneIDi908599.
KEGGimle:ML0200.
PATRICi18050732. VBIMycLep78757_0318.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL023093 Genomic DNA. Translation: CAA18818.1 .
AL583917 Genomic DNA. Translation: CAC29708.1 .
PIRi H86933.
RefSeqi NP_301268.1. NC_002677.1.
WP_010907592.1. NC_002677.1.

3D structure databases

ProteinModelPortali O69548.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272631.ML0200.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAC29708 ; CAC29708 ; CAC29708 .
GeneIDi 908599.
KEGGi mle:ML0200.
PATRICi 18050732. VBIMycLep78757_0318.

Organism-specific databases

Lepromai ML0200.

Phylogenomic databases

eggNOGi COG1754.
HOGENOMi HOG000004020.
KOi K03168.
OMAi ANEGYER.
OrthoDBi EOG6S7XQ9.

Family and domain databases

Gene3Di 1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR025589. Toprim_C_rpt.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF13368. Toprim_C_rpt. 3 hits.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TN.

Entry informationi

Entry nameiTOP1_MYCLE
AccessioniPrimary (citable) accession number: O69548
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: October 29, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3