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Protein

tRNA(Ile)-lysidine synthase

Gene

tilS

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.UniRule annotation

Catalytic activityi

(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H2O.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi33 – 386ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(Ile)-lysidine synthaseUniRule annotation (EC:6.3.4.19UniRule annotation)
Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthaseUniRule annotation
tRNA(Ile)-lysidine synthetaseUniRule annotation
Gene namesi
Name:tilSUniRule annotation
Ordered Locus Names:ML0213
ORF Names:MLCB2548.18c
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML0213.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 323323tRNA(Ile)-lysidine synthasePRO_0000181723Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272631.ML0213.

Structurei

3D structure databases

ProteinModelPortaliO69538.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.

Sequence similaritiesi

Belongs to the tRNA(Ile)-lysidine synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107RD1. Bacteria.
COG0037. LUCA.
HOGENOMiHOG000236508.
KOiK04075.
OMAiPWCVALS.
OrthoDBiPOG091H02FP.

Family and domain databases

CDDicd01992. PP-ATPase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01161. tRNA_Ile_lys_synt. 1 hit.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR011063. TilS/TtcA_N.
IPR012094. tRNA_Ile_lys_synt.
IPR012795. tRNA_Ile_lys_synt_N.
IPR015262. tRNA_Ile_lys_synt_subst-bd.
[Graphical view]
PANTHERiPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamiPF01171. ATP_bind_3. 1 hit.
PF09179. TilS. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02432. lysidine_TilS_N. 1 hit.

Sequencei

Sequence statusi: Complete.

O69538-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRQGAVGQL RRTVEEFAGT YCDIDDQWCV ALSGGSDSLA LTAVAAQLRS
60 70 80 90 100
TTAFIVDHGL QSDSAAVAET ARKQAISLGC VDAQVLCVQI DPPFGPKGGL
110 120 130 140 150
EAAARAARYA VLGANRNGPV LLAHTLDDQA ETVLLGLGRG SGARSIAGMR
160 170 180 190 200
PHDPPWCRPL LGVRRSVPRA ACHELGLNAW QDPHNTDCRY TRTRLRLEVL
210 220 230 240 250
PLLEDVLGGG VVEALARTAT ALREDTELID TFAAQALPNI CAGSGLHARA
260 270 280 290 300
LAALPDAVRR RVIRGWLLNG GATGLTDKQI RGVDTLVTAW RGQGGVAVGS
310 320
TLRGQRLIAS RYDGVLTLHC APV
Length:323
Mass (Da):34,113
Last modified:August 1, 1998 - v1
Checksum:iCA5E345BC863F936
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL023093 Genomic DNA. Translation: CAA18805.1.
AL583917 Genomic DNA. Translation: CAC29721.1.
PIRiE86935.
RefSeqiNP_301278.1. NC_002677.1.
WP_010907602.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC29721; CAC29721; CAC29721.
GeneIDi909579.
KEGGimle:ML0213.
PATRICi18050776. VBIMycLep78757_0339.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL023093 Genomic DNA. Translation: CAA18805.1.
AL583917 Genomic DNA. Translation: CAC29721.1.
PIRiE86935.
RefSeqiNP_301278.1. NC_002677.1.
WP_010907602.1. NC_002677.1.

3D structure databases

ProteinModelPortaliO69538.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML0213.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC29721; CAC29721; CAC29721.
GeneIDi909579.
KEGGimle:ML0213.
PATRICi18050776. VBIMycLep78757_0339.

Organism-specific databases

LepromaiML0213.

Phylogenomic databases

eggNOGiENOG4107RD1. Bacteria.
COG0037. LUCA.
HOGENOMiHOG000236508.
KOiK04075.
OMAiPWCVALS.
OrthoDBiPOG091H02FP.

Family and domain databases

CDDicd01992. PP-ATPase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01161. tRNA_Ile_lys_synt. 1 hit.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR011063. TilS/TtcA_N.
IPR012094. tRNA_Ile_lys_synt.
IPR012795. tRNA_Ile_lys_synt_N.
IPR015262. tRNA_Ile_lys_synt_subst-bd.
[Graphical view]
PANTHERiPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamiPF01171. ATP_bind_3. 1 hit.
PF09179. TilS. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02432. lysidine_TilS_N. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTILS_MYCLE
AccessioniPrimary (citable) accession number: O69538
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.