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Protein

Hypoxanthine-guanine phosphoribosyltransferase

Gene

hpt

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei126 – 1261Proton acceptorBy similarity
Binding sitei154 – 1541IMPBy similarity
Binding sitei176 – 1761IMP; via carbonyl oxygenBy similarity
Metal bindingi182 – 1821MagnesiumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi122 – 13110IMPBy similarity
Nucleotide bindingi181 – 1822IMPBy similarity

GO - Molecular functioni

  1. guanine phosphoribosyltransferase activity Source: UniProtKB-EC
  2. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  1. IMP salvage Source: UniProtKB-UniPathway
  2. purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00591; UER00648.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HGPRT
Short name:
HGPRTase
Gene namesi
Name:hpt
Synonyms:hprT
Ordered Locus Names:ML0214
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML0214.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 203203Hypoxanthine-guanine phosphoribosyltransferasePRO_0000139608Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272631.ML0214.

Structurei

3D structure databases

ProteinModelPortaliO69537.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0634.
HOGENOMiHOG000236520.
KOiK00760.
OrthoDBiEOG693GNP.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O69537-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVAQSFSAI NPGPTAELYL GDIKSVMLTQ EQIQARIAEL GAQIGHDYRD
60 70 80 90 100
RSPASDQDLL LVTVLKGAVI FVTDLARAIP LPTQFEFMAV SSYGSSMSSS
110 120 130 140 150
GVVRILKDLD RDIHDRNVLI VEDVVDSGLT LSWLLRNLAT RRPQSLQVCT
160 170 180 190 200
LLRKPDALRA NVDITYVGFD IPNDFVVGYG LDYDERYRDL PYIGMLDPRV

YQQ
Length:203
Mass (Da):22,665
Last modified:August 28, 2001 - v2
Checksum:iAC9133B56D09EC7A
GO

Sequence cautioni

The sequence CAA18804.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAC29722.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL023093 Genomic DNA. Translation: CAA18804.1. Different initiation.
AL583917 Genomic DNA. Translation: CAC29722.1. Different initiation.
PIRiF86935.
RefSeqiNP_301279.1. NC_002677.1.

Genome annotation databases

GeneIDi909576.
KEGGimle:ML0214.
PATRICi18050778. VBIMycLep78757_0340.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL023093 Genomic DNA. Translation: CAA18804.1. Different initiation.
AL583917 Genomic DNA. Translation: CAC29722.1. Different initiation.
PIRiF86935.
RefSeqiNP_301279.1. NC_002677.1.

3D structure databases

ProteinModelPortaliO69537.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML0214.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi909576.
KEGGimle:ML0214.
PATRICi18050778. VBIMycLep78757_0340.

Organism-specific databases

LepromaiML0214.

Phylogenomic databases

eggNOGiCOG0634.
HOGENOMiHOG000236520.
KOiK00760.
OrthoDBiEOG693GNP.

Enzyme and pathway databases

UniPathwayiUPA00591; UER00648.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TN.

Entry informationi

Entry nameiHPRT_MYCLE
AccessioniPrimary (citable) accession number: O69537
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 28, 2001
Last sequence update: August 28, 2001
Last modified: March 31, 2015
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.