Reviewed, UniProtKB/Swiss-Prot O69473 (FAB2_MYCLE)

Names and origin

Protein names

Recommended name:
    3-oxoacyl-[acyl-carrier-protein] synthase 2
    EC=2.3.1.41
Alternative name(s):
    Beta-ketoacyl-ACP synthase 2
      Short name=KAS 2

Gene names

Name:	kasB
Ordered Locus Names:	ML1656
ORF Names:	MLCB1243.19c

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP By similarity.
Catalytic activity	Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein].
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the beta-ketoacyl-ACP synthases family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-oxoacyl-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	3-oxoacyl-[acyl-carrier-protein] synthase 2		PRO_0000180332
Sites
	Active site	173	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O69473-1 [UniParc]. Last modified August 14, 2001. Version 2. Checksum: C015CF9CC5DC7E72	FASTA	420	44,497
	10 20 30 40 50 60 MTTSPELVTG KAFPNVVVTG IAMTTALATD AETTWKLLLD NQSGIRMLDD PFIEEFNLPV 70 80 90 100 110 120 RIGGHLLEEF DHQLTRVELR RMGYLQRMST VLSRRLWENA GSPEVDTNRL MVSIGTGLGS 130 140 150 160 170 180 AEELVFSYDD MRARGMKAVS PLAVQKYMPN GAAAAVGLEH HAKAGVMTPV SACASGSEAI 190 200 210 220 230 240 AHAWQQIVLG EADSAICGGV ETKIEAVPIA GFSQMRIVMS TKNDNPAGAC RPFDRDRDGF 250 260 270 280 290 300 VFGEAGALML IETEDSAKAR SANILARIMG ASITSDGFHM VAPDPNGERA GHAIARAVHL 310 320 330 340 350 360 AGLSPSDIDH VNAHATGTQV GDLAEAKAIN KALCNNRPAV YAPKSALGHS VGAVGAVESI 370 380 390 400 410 420 LTVLALRDQV IPPTLNLVNL DPDIDLDVVA GKPRPGDYRY AVNNSFGFGG HNVAIAFGCY

References

[1]

"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL023635 Genomic DNA. Translation: CAA19200.1. Different initiation. AL583922 Genomic DNA. Translation: CAC30607.1. Different initiation.
PIR	T44710.
RefSeq	NP_302137.1.
3D structure databases
HSSP	HSSP built from PDB template 1E5M based on UniProtKB P73283.
SMR	O69473. Positions 7-420.
ModBase	Search...
Genome annotation databases
GeneID	910002.
GenomeReviews	Gene locus ML1656 in contig AL450380_GR.
KEGG	mle:ML1656.
NMPDR	fig|272631.1.peg.1009.
Organism-specific databases
Leproma	ML1656.
CMR	Search...
Phylogenomic databases
HOGENOM	O69473.
Enzyme and pathway databases
BioCyc	MLEP272631:ML1656-MON.
BRENDA	2.3.1.41. 808.
Family and domain databases
InterPro	IPR000794. Beta-ketoacyl_synthase. IPR018201. Ketoacyl_synth_AS. IPR014031. Ketoacyl_synth_C. IPR014030. Ketoacyl_synth_N. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 2 hits.
PANTHER	PTHR11712. Ketoacyl_synth. 1 hit.
Pfam	PF00109. ketoacyl-synt. 1 hit. PF02801. Ketoacyl-synt_C. 1 hit. [Graphical view]
PROSITE	PS00606. B_KETOACYL_SYNTHASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FAB2_MYCLE

Accession

Primary (citable) accession number: O69473

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 14, 2001
Last sequence update:	August 14, 2001
Last modified:	June 16, 2009
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)