O69469 (RNC_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease 3
EC=3.1.26.3

Alternative name(s):
Ribonuclease III
Short name=RNase III

Gene names

Name:	rnc
Ordered Locus Names:	ML1659
ORF Names:	MLCB1243.15

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Digests double-stranded RNA. Involved in the processing of ribosomal RNA precursors and of some mRNAs By similarity. HAMAP MF_00104
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00104
Subcellular location	Cytoplasm By similarity HAMAP MF_00104.
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 RNase III domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro rRNA catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro ribonuclease III activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	Ribonuclease 3 HAMAP MF_00104		PRO_0000180412
Regions
	Domain	19 – 134	116	RNase III
	Domain	161 – 229	69	DRBM

Sequences

	Sequence		Length	Mass (Da)
	O69469 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 84BC036362C92AFE	FASTA	238	25,241
	10 20 30 40 50 60 MTQPRQALLD AFGVDLPDEL LSLALTHRSY AYEHGGLPTN ERLEFLGDAV LSLTITDELF 70 80 90 100 110 120 HRHPDRSEGD LAKLRASVVN TQALAYVARN LSDGGLGVYL LLGRGETNTG GADKSSILAD 130 140 150 160 170 180 GMESLLGAIY LHHGIEVARQ VILRLFGTLL DAAPTLGAGL DWKTSLQELT AARGMGVPSY 190 200 210 220 230 VVTSTGPDHD KEFTAVVVVM DTEYGSGIGH SKKEAEQKAA SAAWKALDVL GGVGKTSV

References

[1]

"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL023635 Genomic DNA. Translation: CAA19196.1. AL583923 Genomic DNA. Translation: CAC30612.1.
PIR	T44706.
RefSeq	NP_302140.1. NC_002677.1.
3D structure databases
ProteinModelPortal	O69469.
SMR	O69469. Positions 3-155.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000029261; EBMYCP00000028867; EBMYCG00000029256.
GeneID	910019.
GenomeReviews	Gene locus ML1659 in contig AL450380_GR.
KEGG	mle:ML1659.
NMPDR	fig|272631.1.peg.1012.
PATRIC	18056394. VBIMycLep78757_3128.
Organism-specific databases
Leproma	ML1659.
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000017732.
HOGENOM	HBG744556.
OMA	ALTHKSF.
ProtClustDB	PRK00102.
Enzyme and pathway databases
BioCyc	MLEP272631:ML1659-MONOMER.
Family and domain databases
HAMAP	MF_00104. RNase_III. [Tree]
InterPro	IPR001159. Ds-RNA-bd. IPR014720. dsRNA-bd-like. IPR000999. RNase_III. IPR011907. RNase_III_bac. [Graphical view]
Gene3D	G3DSA:3.30.160.20. dsRNA-bd-like. 1 hit. G3DSA:1.10.1520.10. RNase_III. 1 hit.
KO	K03685.
PANTHER	PTHR11207. PTHR11207. 1 hit.
Pfam	PF00035. dsrm. 1 hit. PF00636. Ribonuclease_3. 1 hit. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00535. RIBOc. 1 hit. [Graphical view]
SUPFAM	SSF69065. RNase_III. 1 hit.
TIGRFAMs	TIGR02191. RNaseIII. 1 hit.
PROSITE	PS50137. DS_RBD. 1 hit. PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNC_MYCLE

Accession

Primary (citable) accession number: O69469

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program