O69395 (BLT2_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-lactamase Toho-2
EC=3.5.2.6

Gene names

Name:

bla

Encoded on

Plasmid IncFII pMTY036

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Hydrolyzes beta-lactam antibiotics such as penicillin G, carbenicillin, cephaloridine, cefoxitin, cefotaxime, ceftazidime, and aztreonam. Has especially increased relative hydrolysis rates for cephalothin, cephaloridine, cefotaxime and ceftizoxime.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Enzyme regulation	Inhibited 16-fold better by the beta-lactamase inhibitor tazobactam than by clavulanic acid.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 289	261	Beta-lactamase Toho-2		PRO_0000016995
Regions
	Region	235 – 237	3	Substrate binding By similarity
Sites
	Active site	73	1	Acyl-ester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O69395 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: A75723E1793060A6	FASTA	289	30,745
	10 20 30 40 50 60 MVTKRVQRMM SAAAACIPLL LGSPTLYAQT SAVQQKLAAL EKSSGGRLGV ALIDTADNTQ 70 80 90 100 110 120 VLYRGDERFP MCSTSKVMAA AAVLKQSETQ KQLLNQPVEI KPADLVNYNP IAEKHVNGTM 130 140 150 160 170 180 TLAELSAAAL QYSDNTAMNK LIAQLGGPGG VTAFARAIGD ETFRLDRTEP TLNTAIPGDP 190 200 210 220 230 240 RDTTTARAGA DVASLRWVMR WAKPSGAVGD VAQRQYDRAA GIRAGLPTSW TVGDKTGSGD 250 260 270 280 YGTTNDIAVI WPQGRAPLVL VTYFTQPQQN AESRRDVLAS AARIIAEGL

References

[1]

"Cloning and sequencing of the gene encoding Toho-2, a class A beta-lactamase preferentially inhibited by tazobactam." Ma L., Ishii Y., Ishiguro M., Matsuzawa H., Yamaguchi K. Antimicrob. Agents Chemother. 42:1181-1186(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: TUM1083.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D89862 Genomic DNA. Translation: BAA28282.1.
3D structure databases
ProteinModelPortal	O69395.
SMR	O69395. Positions 29-289.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR000871. Beta-lactam_class-A/D. IPR023650. Beta-lactam_class-A_AS. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLT2_ECOLX

Accession

Primary (citable) accession number: O69395

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	April 3, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program