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O69395

- BLT2_ECOLX

UniProt

O69395 - BLT2_ECOLX

Protein

Beta-lactamase Toho-2

Gene

bla

Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Hydrolyzes beta-lactam antibiotics such as penicillin G, carbenicillin, cephaloridine, cefoxitin, cefotaxime, ceftazidime, and aztreonam. Has especially increased relative hydrolysis rates for cephalothin, cephaloridine, cefotaxime and ceftizoxime.

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

    Enzyme regulationi

    Inhibited 16-fold better by the beta-lactamase inhibitor tazobactam than by clavulanic acid.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei73 – 731Acyl-ester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. beta-lactamase activity Source: UniProtKB-EC

    GO - Biological processi

    1. beta-lactam antibiotic catabolic process Source: InterPro
    2. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase Toho-2 (EC:3.5.2.6)
    Gene namesi
    Name:bla
    Encoded oniPlasmid IncFII pMTY0360 Publication
    OrganismiEscherichia coli
    Taxonomic identifieri562 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2828Sequence AnalysisAdd
    BLAST
    Chaini29 – 289261Beta-lactamase Toho-2PRO_0000016995Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliO69395.
    SMRiO69395. Positions 29-289.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni235 – 2373Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class-A beta-lactamase family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view]
    PRINTSiPR00118. BLACTAMASEA.
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O69395-1 [UniParc]FASTAAdd to Basket

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    MVTKRVQRMM SAAAACIPLL LGSPTLYAQT SAVQQKLAAL EKSSGGRLGV    50
    ALIDTADNTQ VLYRGDERFP MCSTSKVMAA AAVLKQSETQ KQLLNQPVEI 100
    KPADLVNYNP IAEKHVNGTM TLAELSAAAL QYSDNTAMNK LIAQLGGPGG 150
    VTAFARAIGD ETFRLDRTEP TLNTAIPGDP RDTTTARAGA DVASLRWVMR 200
    WAKPSGAVGD VAQRQYDRAA GIRAGLPTSW TVGDKTGSGD YGTTNDIAVI 250
    WPQGRAPLVL VTYFTQPQQN AESRRDVLAS AARIIAEGL 289
    Length:289
    Mass (Da):30,745
    Last modified:August 1, 1998 - v1
    Checksum:iA75723E1793060A6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D89862 Genomic DNA. Translation: BAA28282.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D89862 Genomic DNA. Translation: BAA28282.1 .

    3D structure databases

    ProteinModelPortali O69395.
    SMRi O69395. Positions 29-289.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view ]
    PRINTSi PR00118. BLACTAMASEA.
    SUPFAMi SSF56601. SSF56601. 1 hit.
    PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of the gene encoding Toho-2, a class A beta-lactamase preferentially inhibited by tazobactam."
      Ma L., Ishii Y., Ishiguro M., Matsuzawa H., Yamaguchi K.
      Antimicrob. Agents Chemother. 42:1181-1186(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: TUM1083.

    Entry informationi

    Entry nameiBLT2_ECOLX
    AccessioniPrimary (citable) accession number: O69395
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Plasmid

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3