Reviewed, UniProtKB/Swiss-Prot O69300 (LON_CAMJE)

Names and origin

Protein names

Recommended name:
    ATP-dependent protease La
    EC=3.4.21.53

Gene names

Name:	lon
Ordered Locus Names:	Cj1073c

Organism

Campylobacter jejuni [Complete proteome] [HAMAP]

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	791 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Degrades short-lived regulatory and abnormal proteins in presence of ATP. Hydrolyzes two ATPs for each peptide bond cleaved in the protein substrate By similarity.
Catalytic activity	Hydrolysis of proteins in presence of ATP.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase S16 family. Contains 1 Lon domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent peptidase activity Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 791	791	ATP-dependent protease La		PRO_0000076128
Regions
	Domain	12 – 201	190	Lon
	Nucleotide binding	364 – 371	8	ATP Potential
Sites
	Active site	697	1	By similarity
	Active site	740	1	By similarity
Experimental info
	Sequence conflict	302	1	I → V in CAA76672. Ref.1
	Sequence conflict	531	1	S → F in CAA76672. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O69300-1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 970A3C6E84BBE600	FASTA	791	90,100
	10 20 30 40 50 60 MQIEEIQNYP ANLPVLVEDE LFLYPFMITP IFINDSSNMK ALDLAIKNDS MLFVAPSKLE 70 80 90 100 110 120 NGRNFDEIYN CGVIGTIMRK VPLPDGRVKI LFQGYAKGKI IEQISNKPLE AKIELIKEDF 130 140 150 160 170 180 LEGTKKEALL EVLKEKVKNL ANISHYFSPD LLRTIEEGFD ASRICDLILN TVRIKKQVAY 190 200 210 220 230 240 EFFVLTDLEQ KLVKLIDLIA QEIEANKIQK EIKNKVHSRI DKVNKEYFLK EQLRQIQKEL 250 260 270 280 290 300 GSDTQKEDEV REYQKRLELK KKFMHEDAYK EIKKQIEKFE RIHQDNSEAS MIQTYIETAL 310 320 330 340 350 360 DIPFEKISKK KLDIKEVSKQ LNHDHYALNK PKERIEEYFA VRELLEKRKI AEKDGAKVIL 370 380 390 400 410 420 CLYGPPGVGK TSLANSVSKA LKRELIRIAL GGLEDVNELR GHRRTYIGAM PGRITQGLIE 430 440 450 460 470 480 AKQINPVIVL DEIDKLNRSF RGDPSAVLLE ILDPEQNSKF RDYYLNFNID LSKVIFIATA 490 500 510 520 530 540 NDISNIPAPL RDRMEFIELS SYTPSEKFHI MKKYLIPDEL KKHGLKSNEL SIDDETIELI 550 560 570 580 590 600 ISDYTRESGV RNLRRKVAEL CRKSAKKLLL ENIKKVIINT KNLNEFLDKK VFEIEKNNGE 610 620 630 640 650 660 NQVGQVNGLA WTSVGGDVLK VEAVKIKGKG ELTLTGSLGD VMKESARIAF SMIKVLIDEG 670 680 690 700 710 720 KIKIPKKIII DPKVNVYDSY NIHIHVPDGA TPKDGPSAGI TISTAIASIF SDKKVKADVA 730 740 750 760 770 780 MTGEIDLKGK VLPIGGLKEK LIAAYKADIK TALIPRKNYE RDLKDIPSEV RDNMEIIAVD 790 TFSDVLEYTL V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of the Campylobacter jejuni lon gene detected by RNA arbitrarily primed PCR." Thies F.L., Hartung H.-P., Giegerich G. FEMS Microbiol. Lett. 165:329-334(1998) [PubMed: 9742705] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y17166 Genomic DNA. Translation: CAA76672.1. AL111168 Genomic DNA. Translation: CAL35190.1.
PIR	D81310.
RefSeq	YP_002344466.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O69300. 1 interaction.
Protein family/group databases
MEROPS	S16.001.
Genome annotation databases
GeneID	905364.
GenomeReviews	Gene locus Cj1073c in contig AL111168_GR.
KEGG	cje:Cj1073c.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O69300.
OMA	O69300. IVNGLAW.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ1073C-MON.
BRENDA	3.4.21.53. 255835.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR008269. Pept_S16_C. IPR004815. Pept_S16_lon. IPR003111. Pept_S16_N. IPR008268. Peptidase_S16_AS. IPR001984. Peptidase_S16_C. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF02190. LON. 1 hit. PF05362. Lon_C. 1 hit. [Graphical view]
PRINTS	PR00830. ENDOLAPTASE.
SMART	SM00382. AAA. 1 hit. SM00464. LON. 1 hit. [Graphical view]
TIGRFAMs	TIGR00763. lon. 1 hit.
PROSITE	PS01046. LON_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LON_CAMJE

Accession

Primary (citable) accession number: O69300
Secondary accession number(s): Q0P9I1, Q9PNM1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	December 1, 2000
Last modified:	June 16, 2009
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)