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Protein

Lon protease

Gene

lon

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner.UniRule annotation

Catalytic activityi

Hydrolysis of proteins in presence of ATP.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei697 – 6971UniRule annotation
Active sitei740 – 7401UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi364 – 3718ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-1046-MONOMER.

Protein family/group databases

MEROPSiS16.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Lon proteaseUniRule annotation (EC:3.4.21.53UniRule annotation)
Alternative name(s):
ATP-dependent protease LaUniRule annotation
Gene namesi
Name:lonUniRule annotation
Ordered Locus Names:Cj1073c
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000000799 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 791791Lon proteasePRO_0000076128Add
BLAST

Proteomic databases

PaxDbiO69300.

Expressioni

Inductioni

By heat shock.UniRule annotation1 Publication

Interactioni

Subunit structurei

Homohexamer. Organized in a ring with a central cavity.UniRule annotation

Protein-protein interaction databases

IntActiO69300. 1 interaction.
STRINGi192222.Cj1073c.

Structurei

3D structure databases

ProteinModelPortaliO69300.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 201189Lon N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini600 – 791192Lon proteolyticPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S16 family.UniRule annotation
Contains 1 Lon N-terminal domain.PROSITE-ProRule annotation
Contains 1 Lon proteolytic domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105C6P. Bacteria.
COG0466. LUCA.
HOGENOMiHOG000261410.
KOiK01338.
OMAiEGTFMPG.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01973. lon_bact. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR027543. Lon_bac.
IPR004815. Lon_bac/euk-typ.
IPR027065. Lon_Prtase.
IPR003111. LON_substr-bd_dom.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR008268. Peptidase_S16_AS.
IPR015947. PUA-like_domain.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10046. PTHR10046. 1 hit.
PfamiPF00004. AAA. 1 hit.
PF05362. Lon_C. 1 hit.
PF02190. LON_substr_bdg. 1 hit.
[Graphical view]
PIRSFiPIRSF001174. Lon_proteas. 1 hit.
SMARTiSM00382. AAA. 1 hit.
SM00464. LON. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00763. lon. 1 hit.
PROSITEiPS51787. LON_N. 1 hit.
PS51786. LON_PROTEOLYTIC. 1 hit.
PS01046. LON_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O69300-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQIEEIQNYP ANLPVLVEDE LFLYPFMITP IFINDSSNMK ALDLAIKNDS
60 70 80 90 100
MLFVAPSKLE NGRNFDEIYN CGVIGTIMRK VPLPDGRVKI LFQGYAKGKI
110 120 130 140 150
IEQISNKPLE AKIELIKEDF LEGTKKEALL EVLKEKVKNL ANISHYFSPD
160 170 180 190 200
LLRTIEEGFD ASRICDLILN TVRIKKQVAY EFFVLTDLEQ KLVKLIDLIA
210 220 230 240 250
QEIEANKIQK EIKNKVHSRI DKVNKEYFLK EQLRQIQKEL GSDTQKEDEV
260 270 280 290 300
REYQKRLELK KKFMHEDAYK EIKKQIEKFE RIHQDNSEAS MIQTYIETAL
310 320 330 340 350
DIPFEKISKK KLDIKEVSKQ LNHDHYALNK PKERIEEYFA VRELLEKRKI
360 370 380 390 400
AEKDGAKVIL CLYGPPGVGK TSLANSVSKA LKRELIRIAL GGLEDVNELR
410 420 430 440 450
GHRRTYIGAM PGRITQGLIE AKQINPVIVL DEIDKLNRSF RGDPSAVLLE
460 470 480 490 500
ILDPEQNSKF RDYYLNFNID LSKVIFIATA NDISNIPAPL RDRMEFIELS
510 520 530 540 550
SYTPSEKFHI MKKYLIPDEL KKHGLKSNEL SIDDETIELI ISDYTRESGV
560 570 580 590 600
RNLRRKVAEL CRKSAKKLLL ENIKKVIINT KNLNEFLDKK VFEIEKNNGE
610 620 630 640 650
NQVGQVNGLA WTSVGGDVLK VEAVKIKGKG ELTLTGSLGD VMKESARIAF
660 670 680 690 700
SMIKVLIDEG KIKIPKKIII DPKVNVYDSY NIHIHVPDGA TPKDGPSAGI
710 720 730 740 750
TISTAIASIF SDKKVKADVA MTGEIDLKGK VLPIGGLKEK LIAAYKADIK
760 770 780 790
TALIPRKNYE RDLKDIPSEV RDNMEIIAVD TFSDVLEYTL V
Length:791
Mass (Da):90,100
Last modified:December 1, 2000 - v2
Checksum:i970A3C6E84BBE600
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti302 – 3021I → V in CAA76672 (PubMed:9742705).Curated
Sequence conflicti531 – 5311S → F in CAA76672 (PubMed:9742705).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y17166 Genomic DNA. Translation: CAA76672.1.
AL111168 Genomic DNA. Translation: CAL35190.1.
PIRiD81310.
RefSeqiWP_002868636.1. NC_002163.1.
YP_002344466.1. NC_002163.1.

Genome annotation databases

EnsemblBacteriaiCAL35190; CAL35190; Cj1073c.
GeneIDi905364.
KEGGicje:Cj1073c.
PATRICi20059104. VBICamJej33762_1055.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y17166 Genomic DNA. Translation: CAA76672.1.
AL111168 Genomic DNA. Translation: CAL35190.1.
PIRiD81310.
RefSeqiWP_002868636.1. NC_002163.1.
YP_002344466.1. NC_002163.1.

3D structure databases

ProteinModelPortaliO69300.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO69300. 1 interaction.
STRINGi192222.Cj1073c.

Protein family/group databases

MEROPSiS16.001.

Proteomic databases

PaxDbiO69300.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL35190; CAL35190; Cj1073c.
GeneIDi905364.
KEGGicje:Cj1073c.
PATRICi20059104. VBICamJej33762_1055.

Phylogenomic databases

eggNOGiENOG4105C6P. Bacteria.
COG0466. LUCA.
HOGENOMiHOG000261410.
KOiK01338.
OMAiEGTFMPG.

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-1046-MONOMER.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01973. lon_bact. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR027543. Lon_bac.
IPR004815. Lon_bac/euk-typ.
IPR027065. Lon_Prtase.
IPR003111. LON_substr-bd_dom.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR008268. Peptidase_S16_AS.
IPR015947. PUA-like_domain.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10046. PTHR10046. 1 hit.
PfamiPF00004. AAA. 1 hit.
PF05362. Lon_C. 1 hit.
PF02190. LON_substr_bdg. 1 hit.
[Graphical view]
PIRSFiPIRSF001174. Lon_proteas. 1 hit.
SMARTiSM00382. AAA. 1 hit.
SM00464. LON. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00763. lon. 1 hit.
PROSITEiPS51787. LON_N. 1 hit.
PS51786. LON_PROTEOLYTIC. 1 hit.
PS01046. LON_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLON_CAMJE
AccessioniPrimary (citable) accession number: O69300
Secondary accession number(s): Q0P9I1, Q9PNM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 1, 2000
Last modified: September 7, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.